One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures〔10% (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.〕which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into LN2. One-step dilution in straw was done in 25℃ water for 1 min, by placing vertically in the state of plugged- end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

UV light irradiation is known to give beneficial effects on fresh produce preservation. A container system equipped with UV-LED was fabricated for storing cherry tomatoes under computer-controlled conditions of intermittent on-off cycles (1 hour on/1 hour off). Wavelength (365 and 405 nm) and physical location of the LED (2 and 5 cm above fruit) were studied as variables affecting the respiration, ethylene production and quality preservation of the fruits at 10 and 20℃. 365 nm wavelength gave much higher radiation intensity than 405 nm, and intensity on surface decreased in inverse proportion to square of distance from LED. When compared to non-irradiated control, UV-LED irradiation decreased the respiration by 5-10% at 10℃ while there was no obvious effect at 20℃. Ethylene production was reduced when the fruits were placed at 5 cm distance, while there was no significant difference from control at 2 cm location. The reduction of ethylene production at 5 cm was more pronounced at 20℃. UV-LED irradiation was shown to have delayed increase or lower concentration in carotenoids compared to control treatment. Any negative effect of UV-LED irradiation on ascorbic acid content and firmness was not observed.

Cheonsu is a new japonica rice cultivar tolerant to drought. This cultivar was developed from the cross Ilmibyeo// Juanbyeo/Salshare, where the parent Ilmibyeo is a japonica cultivar with high eating quality, Juanbyeo is a japonica for direct-seeding cultivation, and Salshare is a japonica weedy rice from Korea. The preliminary and replicated yield trail on Cheonsu was carried out at Yeungnam University in 2001, 2002 and 2003, and the local adaptability test was carried out by the National Seed Management Office (NSMO), Korea, in 2004 and 2005. This cultivar was registered to NSMO, Korea, as variety Cheonsu in 2006. Cheonsu has about 122 days growth duration from transplanting to harvesting. This cultivar has stature with 81cm of culm length. The milled rice yield of Cheonsu is about 5.10 MT/ha under standard fertilizer level of the ordinary transplanting cultivation. Amylose content of this cultivar is 18.3%.

Sanho is a new japonica rice cultivar with brown pericarp color (Fig. 1). This cultivar was developed from the cross Ilmibyeo//Ilmibyeo/Pyeongtaekaengmi 4, where the recurrent parent Ilmibyeo is a japonica cultivar with high eating quality, and the donor