As the size of the gaming market continues to grow, game engines like Unity and Unreal are constantly being developed and updated for multi-platform support. In particular, Unity divides the Render Pipeline to be used. Among them, the Universal Render Pipeline (URP) has the advantage of supporting various platforms while improving performance by reducing draw calls and batches at the same quality settings and reducing the load on the CPU and GPU. However, only limited global illumination is supported in the real-time 3D rendering area within URP, such as Baked GI and Enlighten GI, and Enlighten GI will be deprecated after 2024LST, so real-time global illumination is not available in URP, which may reduce the realism and immersion of global illumination in games or applications that use URP. The main objective of this research is to incorporate precomputed spherical harmonics into URP to enable dynamic GI updates in real-time. The approach proposed in this research is to compute the coefficients of spherical harmonics offline and then run them within the URP rendering pipeline, where work exists to effectively simulate dynamic lighting conditions. To demonstrate this, experiments were conducted by changing the rotation and color of the lights, and a comparative analysis was performed to demonstrate the effectiveness of the theory proposed in this work.

최근 AI 기술은 하루가 다르게 빠르게 발전하고 있고, AI기술은 각 분야에서 다양하게 사용되어지고 있다. 본 논문은 예술분야에서 AI기술의 활용으로 COVID-19 상황에서 인간관계, 개인적인 이유로 지친 마음을 위 로해주는 힐링 게임을 제작하였다. 제작한 힐링게임에서는 주로 Self-help-therapy의 효과를 얻을 수 있어, 치 료자의 도움없이 이용자가 힐링게임을 통하여 일상적 이용과정에서 치유적 효과를 얻을 수 있는 것을 기대 하고 있다. 게임 리뷰 데이터를 통계 분석하여 힐링게임으로 대중들이 요구하는 부분을 수용하여 힐링게임 이 제작되었으며, 사용자는 게임 시작 전 간단한 스토리라인과 AI와 상호작용할 수 있는 간단한 대화를 통 화여 Self-help-therapy 효과를 얻을 수 있었다.

How do people aware of the role of the zoo, and the zoo had previously had their effects on the image, visit the zoo to determine the factors that influence the need for research on what is felt. The purpose of this study is intended for students and parents before and after the program Zoos intimacy and excitement in any situation that action says about the attributes, intimacy (emotional interest) is saying about the differences appear. Experience program before and after the study to participants interested in the cause, but did not significantly differ Jean. Education, safety, sanitation, etc. are not taken into account the situation of the experient live antipathy to the conclusion that it came out.

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures〔10% (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.〕which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into LN2. One-step dilution in straw was done in 25℃ water for 1 min, by placing vertically in the state of plugged- end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

This study was to investigate the effect of flavonoid treatment on in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos, and their pregnancy and delivery rate after embryo transfer into recipient. In experiment 1, to optimize the flavonoid concentration, parthenogenetic day 2 (≥ 2-cell) embryos were cultured in 0 (control), 1, 10 and 20 μM flavonoid for 6 days. In the results, in vitro development rate was the highest in 10 μM flavonoid group (57.1%) among treatment groups (control, 49.5%; 1 μM, 54.2%; 20 μM, 37.5%), and numbers of total and ICM cells were significantly (p<0.05) higher in 10 μM flavonoid group than other groups. We found that 10 μM flavonoid treatment can significantly (p<0.05) decrease the apoptotic index and derive high expression of anti-oxidant, anti-apoptotic, cell growth and development marker genes such as Mn-SOD, Survivin, Bax inhibitor, Glut-5, In-tau, compared to control group. In experiment 2, to produce the cloned Jeju Black Cattle, beef quality index grade 1 bull somatic cells were transferred into enucleated bovine MII oocytes and reconstructed embryos were cultured in 10 μM flavonoid added medium. When the in vitro produced day 7 or 8 SCNT blastocysts were transferred into a number of recipients, 10 μM flavonoid treatment group presented higher pregnancy rate (10.2%, 6/59) than control group (5.9%, 2/34). Total three cloned Jeju Black calves were born. Also, two cloned calves in 10 μM flavonoid group were born and both were all healthy at present, while the one cloned calf born in control group was dead one month after birth. In addition, when the result of short tandem repeat marker analysis of each cloned calf was investigated, microsatellite loci of 11 numbers matched genotype between donor cell and cloned calf tissue. These results demonstrated that the flavonoid addition in culture medium may have beneficial effects on in vitro and in vivo developmental capacity of SCNT embryos and pregnancy rate.

Background : As a part of ongoing research to elucidate and characterize anti-cancer nutraceuticals, extracts from many kinds of medicinal plants were tested for their ability to cytotoxicity on cancer cells so far. Datura stramonium is one of the plants known to contain various alkaloids such as hyoscyamine, scopolamine, atropine etc. Traditionally, it has been used as an analgesic, antispasmodic, and central nervous stimulant. Leaves are also known to be effective against asthma, cough, and chronic bronchitis. In this study, cytotoxicity of extracts from D. stramonium on human cancer cell lines A549 (lung), and HepG2 (liver) were evaluated and compared. Methods and Results : The extract was diluted with DMSO in the form of 10, 50, 250, 1,000 μg/ml for final concentration series respectively. The cell viabilities were examined by MTT assay. On HepG2 cell line, extracts of D. stramonium showed dramatic dose-dependent cytotoxicity on 10, 50, 250, 1,000 ㎍/㎖ concentrations series as 88.16%, 78.55%, 55.57%, 23.06% cell viability respectively. On A549 cell line, likewise, same concentration series showed a dose-dependent cytotoxic effect as 96.49%, 96.12%, 68.54%, 20.26% cell viability respectively. On A549 cell line, there was no difference in effect between 10 ㎍/㎖ and 50 ㎍/㎖. Conclusion : Above results, high concentrations of extracts are effective on two cancer cell lines. These results are expected to be used on further studies about the anticancer activity of D. stramonium as basic data. In order to confirm the anticancer effect of D. stramonium, it is anticipated that additional tests will be required to confirm the apoptosis assay and related protein expression.

Background : In ancient, roots of Rumex crispus, called wooi-daehwang, were used for various symptoms and diseases like cough, phlegm, bronchitis and hepatitis, caused inflammatory. As a part of ongoing research to elucidate and characterize anti-inflammatory nutraceuticals, solvent-partitioned fractions from R. crispus root were tested for their ability to suppress inflammation. In this study, NO synthesis inhibitory activity of solvent-partitioned fractions from R. crispus root on LPS-stimulated RAW264.7 mouse macrophages was evaluated. Methods and Results : The EtOH extracts were suspended in water. The aqueous layer was further partitioned in diethylether, ethylacetate and n-butanol, sequentially. RAW264.7 cells were seeded onto 96-well plates, and cells were allowed to adhere for 6 h and then were pretreated with the R. crispus root extracts for 24 h. Cellular nitric oxide (NO) production was stimulated by adding lipopolysaccharide (LPS). Absorbance was measured at 520 ㎚ by microplate reader. NO synthesis inhibitory activity potential of these fractions was evaluated by assessing NO production by LPS-stimulated RAW264.7 cells in the presence and absence of the solvent-partitioned R. crispus root fractions. NO synthesis inhibitory activity of diethylether fraction diluted in 50 ㎍/㎖, 25 ㎍/㎖, 12.5 ㎍/㎖, 6.25 ㎍/㎖, 3.125 ㎍/㎖ was 79.2%, 70.9%, 59.5%, 16.1%, and 11.8%, respectively. And NO synthesis inhibitory activity range of another fractions, EtOAc, n-BuOH and aqueous layer, were 0 - 30.2%, 0 - 20.1% and 3.8 - 22.4%, respectively. Conclusion : From the above results, it showed that diethylether fraction have strong NO synthesis inhibitory activity, it was suggested that R. crispus root have NO synthesis inhibitory effects. R. crispus root possesses anthraquinones, such as chrysophanol, parietin, and anthrones etc. According to previous studies, R. crispus semen extract has analgesic and hepatoprotective effect as anti-inflammatory, and extract of R. napalensis has cyclooxygenase (COX)-2, COX-1 inhibitory and free radical scavenging effect. Our present study has shown that R. crispus root extracts anti-inflammatory effects probably by suppressing iNOS expressions, and resulting in the inhibition of NO synthesis.

Crohn’s disease is a chronic inflammatory bowel disease, involving gastrointestinal tract and extra-intestinal organs. IgA nephropathy is a rare extra-intestinal manifestation of Crohn’s disease. We describe a case of 21-year old Korean man who was diagnosed with Crohn’s disease 5 years after IgA nephropathy was suspected. At the age of 16, he had gross hematuria and 2 years after he was diagnosed with IgA nephropathy. Three years later, he complained of abdominal pain and diarrhea. He was diagnosed with Crohn’s disease by colonoscopy and histologic exams. There has been increasing evidences of common pathophysiology between the two diseases.

Background : This study was carried out to investigate the cytotoxicity in 9 extracts from 8 medicinal plants, such as leaf extract of Lonicera maackii (Llm), leaf extract of Platycarya strobilacea (Lps), flower extract of Fagopyrum dibortryis (Fdf), stem extract of Physostegia virginiana (Spv), root extract of Allium senescence (Ras), aerial part extract of Allium schoenoprasum (Aas), aerial part extract of Artemisia japonica var. manshurica (Aaj), stem extract of Caryopteris incana (Sci), and leaf extract of Caryopteris incana (Lci), on human cancer cell lines. Methods and Results : Dried plant extracts were granted from National Institute of Horticultural and Herbal Sciences. The extracts of each plant were dissolved in DMSO and stored in deep freeze at –20℃. The cell viabilities were examined by MTT assay. On SK-OV-3 cell line, Lps, Aas, Sci ans Lci showed dose-dependent cytotoxic effect. On A549 cell line, almost samples show dose-dependent cytotoxic effect, but especially Aaj showed relatively high cytotoxic effect. In case of HCT-15 cell line, Llm and Aas showed relatively high cytotoxic effect. Conclusion : These results suggested that Lonicera maackii, Platycarya strobilacea, Fagopyrum dibortryis, Physostegia virginiana, Allium senescence, Allium schoenoprasum, Artemisia japonica var. manshurica, and Caryopteris incana can be utilized as potential sources of anticancer agent due to their cytotoxicity.

Background : As a part of ongoing research to elucidate and characterize anti-inflammatory nutraceuticals, six kinds of plant extracts (aerial part of Nepeta cataria, leaves of Lonicera maackii, leaves of Platycarya strobilacea, flower of Fagopyrum dibotrys, flowers and fruits of Solanum nigrum, stem of Physostegia virginiana) were tested for their ability to suppress inflammation. The anti-inflammatory has been studied in lipopolysaccharide (LPS)-stimulated RAW264.7 cells which cells synthesized nitric oxide (NO) from L-arginine by nitric oxide synthase (NOS). In this study, NO synthesis inhibitory activity of six kinds of plant extracts on LPS-stimulated RAW 264.7 mouse macrophages was evaluated. Methods and Results : Six kinds of plant extracts were parceled out from RDA (Rural Development Administration). RAW 264.7 cells (1.5×105 cells/well) were seeded onto 96-well plates with DMEM media containing 10% FBS and 1% antibiotics. The cells were pretreated with the extracts and LPS-stimulated cells for 24 h. Cellular NO production was stimulated by adding 1 μg/mL of LPS. After incubation, Griess reagent was used to determine NO production. Absorbance was measured at 520 nm by microplate reader. NO synthesis inhibitory activity potential of these extracts was evaluated by assessing NO production by LPS-stimulated RAW 264.7 cells in the presence. As a result, inhibition rate of NO production was about 40% of L. maackii, 33% of F. dibotrys, 23% of P. strobilacea and 17% of P. virginiana. Meanwhile, there was no significant results in aerial part of N. cataria and flowers and fruits of S. nigrum. Conclusion : From the above results, we be able to confirm that leaves of L. maackii and flower of F. dibotrys appeared dose-dependent NO synthesis inhibitory activity and leaves of P. strobilacea appeared NO synthesis inhibitory activity in low-concentration. As screening NO synthesis inhibition of six extracts, they may be a good candidate for delaying the progression of human inflammatory diseases and warrants further studies.

Idiopathic spontaneous intraperitoneal hemorrhage is a rare, often fatal condition; however, the patients present with nonspecific symptoms. Even after a comprehensive radiological examination and surgical exploration, the origin of hemorrhage often remains unclear. A 74-year-old woman with sudden abdominal pain, nausea, and vomiting was admitted to our hospital. She had no history of trauma. Computed tomography showed a huge hematoma along the small intestinal mesentery without evidence of visceral organ abnormality causing bleeding. The hematoma was completely removed by surgery and no origin of hemorrhage was detected. Herein, we report on a case of idiopathic spontaneous intraperitoneal hemorrhage.

분리 대두단백질과 옥수수 전분을 혼합한 복합 필름의 막 특성을 이용하여 메추리알을 코팅하고 저장성 향상 및 품질 유지를 증대시키고자 하였으며 복합 필름을 복합조리식품(샌드위치)에 적용하여 식빵의 품질을 유지하기 위한 저장 실험을 하였다. 메추리알에 필름 용액을 코팅시킨 후 저장 중 Haugh unit yolk index, 중량변화를 측정하였다. 로 조절된 incubator에서 20일간 저장된 대조구와 복합 필름으로 코팅된 메추리알의 Haugh u