본 연구는 식물공장에서 미나리(Oenanthe javanica DC.) 를 초장별(20cm, 30cm, 40cm, 50cm)로 재배할 경우 기능성 물질과 영양학적 가치가 유지되는지를 평가하기 위해 수행되 었다. 미나리는 항산화 효과, 항염증, 간 보호 등 다양한 생리 활성을 갖춘 기능성 채소로, 기존 재배 방식의 한계를 극복하 고 연중 안정적인 생산이 가능한 식물공장 재배 가능성을 확 인하는 것이 본 연구의 목적이다. 연구 결과, 초장 30cm에서 칼륨(K)과 칼슘(Ca) 함량이 가장 높았으며, 비타민 C 함량 또 한 1,165.38mg·kg-1으로 가장 높았다. 총 폴리페놀 함량은 초 장 20-40cm에서 유사한 수준을 유지하였으며, 초장 50cm 에서 감소하는 경향을 보였다. 기능성 물질인 Quercetin, Apigenin, Isorhamnetin 함량은 초장에 따른 변동 폭이 크지 않아, 단기 재배된 초장이 짧은 미나리도 충분한 기능성 물질 을 함유하고 있음을 확인하였다. 식이섬유 함량 또한 모든 초 장에서 일정 수준(300g·kg-1 이상)을 유지하여 건강 기능성 채소로 활용 가능성이 높았다. 본 연구 결과를 종합하면, 식물 공장에서 초장 30cm로 재배된 미나리는 높은 영양 가치와 기 능성을 유지할 수 있으며, 기존 노지재배 방식과 비교해 연중 균일한 생산이 가능하여 산업적 활용 가치가 클 것으로 판단 된다.
본 연구는 식물공장형 육묘시스템에서 광도 조절에 따른 수 박 접목묘의 생육 및 품질을 평가하기 위해 수행되었다. 수박 접수는 고온기 품종 ‘부라보꿀(BK)’, 저온기 품종 ‘산타꿀 (SK)’을 사용하였고, 대목으로 고온기 품종 ‘불로장생(BS)’, 저온기 품종 ‘동장군(DG)’을 사용하였다. 광도는 125 ± 25, 225 ± 25 및 325 ± 25μmol·m-2·s-1 3처리로 조정하여 실험을 진행하였다. 초장은 고온기 접목묘가 125 ± 25μmol·m-2·s-1, 저온기 접목묘가 225 ± 25μmol·m-2·s-1에서 가장 높았고, 줄 기 직경과 마디수는 고온기 접목묘, 저온기 접목묘 모두 225 ± 25μmol·m-2·s-1에서 가장 높았다. 근장과 엽면적은 고온기 접 목묘에서 325 ± 25μmol·m-2·s-1, 저온기 접목묘는 225 ± 25 μmol·m-2·s-1에서 가장 높았다. 또한 지상부 및 지하부 생체중 과 건물중은 225 ± 25μmol·m-2·s-1 처리에서 가장 높게 나타 났다. 충실도는 두 품종 모두 광도가 증가함에 따라 높아졌으 며, 특히 고온기 접목묘에서 그 효과가 두드러졌다. 엽면적비 (LAR)는 고온기 접목묘에서 광도 증가와 함께 유의하게 증가 하였으나, 저온기 접목묘에서 유의한 차이가 없었다. 종합적 으로 생육 특성은 225 ± 25μmol·m-2·s-1에서 높았으나, 묘소 질을 고려했을 때 325 ± 25μmol·m-2·s-1에서 더욱 강건한 묘 가 형성된 것으로 판단된다.
This study aims to propose new grading standards that can be applied to AI-based automatic sorting machines, reflecting current distribution and consumption trends. The current domestic grading standards for onions in South Korea are based on the “Agricultural and Fishery Products Quality Control Act”. They classify onions based on criteria such as uniformity, shape, color, and the presence of foreign matter. Onion grading standards are divided into four categories based on bulb diameter and weight. However, in the actual domestic market, onions are distributed according to a five-grade classification based on bulb diameter. Therefore, this study classified onions into eight grades, reflecting current distribution and consumption trends in the domestic market. These grades are applicable to AI-based automatic sorting machines. Marketable onions were classified into A1 (extra large) to A5 (extra small) based on the diameter of a single bulb. Onions used for non-marketable purposes (processing) were classified as grade B. Additionally, grade C and grade D were designated for processing and disposal, respectively. By establishing quality grading classifications that align with current distribution and consumption market trends as well as the operational characteristics of AI-based automatic sorting machines, we can expect improvements in work efficiency and reductions in distribution costs. Following this study, it will be necessary to establish comprehensive quality grading standards that include both external criteria (such as bulb weight and size) and internal criteria (such as detection of internal decay and disease occurrence).
거저리는 식품으로 사용되기 때문에 유충기가 오래 지속되면 더 좋다. 반면에 거저리의 개체수 유지를 위 해 성장을 가속화하기 위해서는 유충이 빨리 성충이 되면 더 좋다. 이 연구에서는 개체군 밀도가 거저리의 발달 시 간에 미치는 영향을 구명하였다. 이를 위해 상단 7 cm, 하단 5 cm, 높이 3 cm 크기의 용기를 사용했다. 거저리는 용기 당 1, 2, 5, 10, 20마리의 밀도로 용기에서 서식하였다. 용기에 밀기울 1 g을 넣고 거저리의 먹이 여부에 따라 라벨을 붙였다. 실험은 세 번 반복되었다. 모든 실험에서 개체군 밀도가 높을수록 유충에서 번데기로의 변환 시간이 짧았 지만 번데기에서 성충으로 변환되는 시간은 크게 다르지 않았다. 또한 먹이가 있는 그룹에서 번데기로의 변환 시간 이 단축되었지만, 성충으로 변환되는 시간에는 차이가 없었다. 이 연구 결과는 유충기를 연장하기 위해 더 낮은 밀 도가 필요하고 더 빠른 속도로 성충이 필요하다면 밀도가 더 높아야 한다는 것을 보여주었다. 결론적으로 거저리의 발달 시간은 개체수 밀도에 의해 제어할 수 있을 것이다.
High-entropy alloys (HEAs) have been reported to have better properties than conventional materials; however, they are more expensive due to the high cost of their main components. Therefore, research is needed to reduce manufacturing costs. In this study, CoCrFeMnNi HEAs were prepared using metal injection molding (MIM), which is a powder metallurgy process that involves less material waste than machining process. Although the MIM-processed samples were in the face-centered cubic (FCC) phase, porosity remained after sintering at 1200°C, 1250°C, and 1275°C. In this study, the hot isostatic pressing (HIP) process, which considers both temperature (1150°C) and pressure (150 MPa), was adopted to improve the quality of the MIM samples. Although the hardness of the HIP-treated samples decreased slightly and the Mn composition was significantly reduced, the process effectively eliminated many pores that remained after the 1275°C MIM process. The HIP process can improve the quality of the alloy.
This study aimed to determine the optimal planting density for sesame cultivation in a double cropping system after harvesting onion and garlic in the Muan region. It compared the growth, yield, disease susceptibility, and labor hours for two varieties of sesame (Landrace and Geonbaek sesame) at different planting densities. Plant height in the Landrace sesame showed no significant variation with different planting densities, whereas Geonbaek sesame increased in height with higher planting densities. Both cultivars increased the number of viable branches at lower densities. The stem diameter was thicker in Landrace sesame with decreasing planting density, while in the Geonbaek sesame, there was no significant difference in stem diameter regardless of planting density. The number of capsules per plant for the Landrace sesame increased with decreasing density, whereas Geonbaek sesame showed no significant difference. Yield for both cultivars was higher at greater densities. However, the late direct sowing time and shorter cultivation period significantly reduced the yield of sesame cultivated in a double cropping system compared to that of sesame grown in a single cropping system, due to the late direct sowing time and shorter cultivation period. There was no significant difference in disease occurrence based on planting density. Furthermore, although labor hours did not vary with planting density when comparing the labor hours required for cultivating the Landrace sesame and Geonbaek sesame, the latter needed less labor time. This study reveals the optimum planting distance and density for sesame cultivation as a double cropping after harvesting onion and garlic, providing invaluable data for establishing sesame double cropping cultivation techniques.
Despite advancements in therapeutic approaches, radiotherapy and cisplatin-based chemotherapy remain primary noninvasive treatments for patients with oral squamous cell carcinoma (OSCC). Moreover, the 5-year survival rate for patients with OSCC has remained almost unchanged for several decades, and many side effects of chemotherapy still exist. In this study, three-dimensional (3D) models of OSCC were established using fibroblasts, and the efficacy of various biological inhibitors was evaluated. A culture of epithelial cells with two types of fibroblasts (hTERT-hNOFs and cancer-associated fibroblasts) within a type I collagen matrix resulted in the formation of a continuous layer of tightly packed cells compared to models without fibroblasts. Furthermore, the effects of biological chemicals, including Y27632, latrunculin A, and verteporfin, on these models were investigated. The stratified formation of the epithelial layer and invasion in OSCC 3D-culture models were effectively inhibited by verteporfin, whereas invasion was weakly inhibited by Y27632 and latrunculin. Collectively, the developed OSCC 3D-culture models established with fibroblasts demonstrated the potential for drug screening, with verteporfin showing promising efficacy.
본 연구는 딸기의 삽목 육묘 시 묘소질을 향상시키고 품질을 증진시키기 위한 지베렐린 효과를 검증하기 위해 수행되었다. 품종은 ‘설향’을 대상으로 하였고, 삽목 시 삽수의 기부를 30, 60분간 GA3 50, 100, 150mg·L-1에 침지 처리하고 증류수 침 지를 대조구로 두었다. 정식 시 묘소질 조사 결과 엽수와 SPAD 값은 지베렐린 침지시간이 길수록 증가하였고, 초장, 엽병장, 엽장, 엽폭, 엽면적은 침지시간과 농도 간의 상호작용에 따라 유의하게 값이 변하였다. 관부직경은 같은 농도라 할지라도 침지시간에 따른 차이가 나타났으며 30분, 100mg·L-1 처리를 제외하고 모두 직경이 8.0mm 이상으로 우량한 묘가 생성되 었으며, T/R률은 30분, 50mg·L-1 처리에서 유의하게 작아 식 물체 활력이 가장 우수했다. 양자수율은 농도가 150mg·L-1일 때 저조하였고, 지베렐린 처리 농도가 높을수록 양자수율이 감소하는 경향을 나타냈다. NPQ는 대조구 대비 30분, 150mg·L-1 처리에서 유의하게 작아 묘 스트레스 감소에 효과 가 있었다. 항산화물 함량은 30분, 50mg·L-1 처리와 60분, 150mg·L-1 처리에서 높게 나타났고, 정식 후 생육조사 결과 화아분화 정도에 미치는 지베렐린의 부정적 영향은 없었다. 따라서, ‘설향’ 딸기의 삽목 육묘 시 지베렐린 처리는 정식묘 양성에 효과가 있는 것을 확인할 수 있었으며, 이때 대조구 대 비 생육 증진 및 품질 향상을 위해서는 삽수 기부를 50mg·L-1 의 지베렐린에 30분 침지처리하는 것이 보다 적정한 것으로 판단되었다.
This study was conducted to investigate the growth characteristics of domestic 'Sulhyang' strawberry (Fragaria × ananassa Duch.) seedlings and to analyze their relationships in order to develop a growth prediction model. Fresh weight, dry weight, and leaf area were measured to validate the newly developed growth model. The relative growth rate (RGR) of ‘Sulhyang’ seedlings’ dry weight was an average of 0.026 g·g-1·d-1, and it increased to 0.066 g·g-1·d-1 on the 49th day after transplanting (DAT). The relationship between DAT and RGR was represented as RGR (dry weight)(g·g- 1·d-1) = 0.0392/(1 + exp(–(DAT – 34.9940)/5.8662)). The crop growth rate (CGR) was an average of 0.060 g·m-2·d-1, and it increased to 0.211 g·m-2·d-1 on the 70th DAT. The relationship between DAT and CGR was calculated as CGR (dry weight)(g·m-2·d-1) = 0.1293/(1 + exp(–(DAT – 49.3917)/6.0928)). The relationship between shoot fresh weight (y) and shoot dry weight (x) per plant showed a linear relationship of y = 4.3189x + 0.7812 (R2 = 0.9976). Fresh weight, dry weight, and leaf area with respect to DAT and cumulative temperature increased exponentially, and sigmoid curve models were developed based on these data. The model with the highest coefficient of determination was found for the relationship between shoot dry weight (y) and cumulative temperature (x), represented as y = 14.2285/(1 + exp(–(x – 1590.1295)/377.8112)) (R2 = 0.9715). The results of this study can be utilized as valuable information for establishing a systematic management system for seedling production using strawberry cutting propagation methods. For the development of a more precise growth prediction model in the future, it is necessary to analyze and apply a wider range of growth indicators and meteorological factors related to strawberry seedlings.
This research was conducted to analyze the relationship between the induction time of runners and the growing degree days for domestic strawberry cultivars, 'Sulhyang' and 'Arihyang'. Runners were induced at 15-day intervals over five periods, starting from late April to early June, and the study compared the number of runner formations and temperature changes for each period while it also investigated the plantlet quality on the transplanting date. Based on back calculations from the transplanting date of September 18th, 'Sulhyang' required induction from late April to early May to achieve 70-day-old plantlets and from late April to mid-May for 60-day-old plantlets. On the other hand, 'Arihyang' needed induction from late April for 70-day-old plantlets and from late April to early May for 60-day-old plantlets. To secure about 20 daughter plants, the growing degree days needed to be around 1,000°C, and a delay in runner induction led to a delay in formation of daughter plants. The relationship between growing degree days and runner formation was expressed as the following quadratic equations: for 'Sulhyang,' y = 9E-06x2 + 0.0124x – 2.4806 (R2 = 0.9696), and for 'Arihyang,' y = 1E-06x2 + 0.0192x – 2.9274 (R2 = 0.9431), and these equations showed high correlations. The crown diameter on the transplanting date was thickest in mid-May for 'Sulhyang', and it tended to increase with delayed runner induction for 'Arihyang'. In conclusion, this study confirmed that the number of daughter plants and growth characteristics varied depending on strawberry cultivars and the timing of runner inducement time. The relationship equations derived through measurements of growing degree days can be used as the fundamental data for reasonable production of daughter plants in the future.
This study was conducted to identify the optimal root zone temperature for paprika cultivation, with an aim to increase the heating and cooling energy efficiency and prepare for extreme weather conditions. The greenhouse air temperature was maintained at 20oC and 25oC during the daytime (12 hours) and at 18oC during the nighttime (12 hours). The plant height did not show any significant differences between the treatment with air temperature and root zone temperature. The root length was highest under an air temperature of 25oC with root zone temperatures of 25oC and 30oC, and it was the lowest at 15oC. The leaf number was the highest when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The leaf area increased with higher root zone temperatures, but considering the compactness of the seedlings, a root zone temperature of 25oC was found to be the most effective. The fresh and dry weight of the shoot increased with higher root zone temperatures at an air temperature of 25oC, while the fresh and dry weight of the roots tended to be higher when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The compactness was most effective when the root zone temperature was adjusted to 20oC and 25oCC across all air temperatures. Based on the above results, adjusting the root zone temperature to 25oC at an air temperature of 25oC was found to be effective for the early growth of Paprika. The results of this study suggest that not only can growth be promoted through the regulation of root zone temperature, but it also contribute to the establishment of root zone temperature control technology, which can prevent an excessive drop and rise in the root zone temperature.
This study compared the physicochemical properties of soybean curd residue and black soybean curd residue produced by hot air-drying and freeze-drying. Regardless of drying method, the crude protein, crude ash, crude fiber contents, pH, L, a, b color values and water soluble index were higher in soybean curd residue, whereas total polyphenol contents and antioxidant activity were higher in black soybean curd residue. Significant differences in water absorption index, oil absorption capacity and emulsion activity were observed between soybean curd residue and black soybean curd residue in freeze-drying. On the other hand, the emulsion stability was not significant difference in both hot-air drying and freezedrying. The crude protein and crude fiber contents of soybean curd residue were not significant difference between hot-air drying and freeze-drying. Freeze-drying resulted in higher crude ash contents, pH, water absorption index, water soluble index, oil absorption capacity, emulsion activity and emulsion stability than hot-air drying. Hot-air drying have caused significantly higher water contents, water activity, total polyphenol contents and antioxidant activity in soybean curd residue than freeze-drying. In conclusion, soybean type and drying methods affect the physicochemical and quality characteristics of soybean curd residue, which could be important factors in the manufacture of processed foods.
This study was conducted to determine the optimal dipping time and concentration of gibberellin for improving the growth and quality of domestic cultivar 'Seolhyang' strawberry when using runner plants. Strawberry runner plants were collected on November 10th and soaked in GA3 concentrations of 50, 100, and 150 mg·L-1 for 30 and 60 minutes, respectively. After 75 days of planting, the growth results showed that in the 30-minute, 50 mg·L-1 treatment, the crown diameter was thicker and the T/R ratio was lower, indicating better plant vitality. Runner length increased with lower gibberellin concentrations, particularly promoting vegetative growth. Photosynthetic efficiency was more influenced by gibberellin concentration than dipping time, and using concentrations above a certain threshold acted as a stress factor for runner plants, leading to decreased photosynthetic efficiency. For enhancing seedling growth, soaking with 50 mg·L-1 of gibberellin for 30 minutes was found to be optimal. This study verified the effects of gibberellin treatment on strawberry runner plants to improve plant growth and quality, providing useful basic data for using gibberellin.
주변 국가인 태국, 캄보디아, 베트남, 라오스 등에서 벼멸구(Nilaparvata lugens)와 흰등멸구(Sogatella furcifera)를 채집하던 중, 벼멸구와 형태가 아주 유사한 이삭멸구(N. muiri)와 벼멸구붙이(N. bakeri), 그리고 흰등 멸구와 형태가 아주 유사한 흰등멸구붙이(S. kolophon), 피멸구(S. vibix) 그리고 애멸구(Laodelphax striatellus)가 동시에 채집이 되는 등 형태적 차이점이 거의 없어 전문가도 쉽게 구분하지 못하는 문제가 있음이 확인되었다. 따라서 형태가 유사한 상기 멸구류의 종 동정을 확실히 할 수 있는 PCR용 프라이머의 개발을 위해 벼멸구 및 흰등멸구의 미토콘드리아 내 COI 영역을 특이적으로 검출할 수 있는 프라이머 세트를 제작하고 종 동정 효과를 확인하였다.
The growth characteristics according to temperature conditions were investigated in the breeding room of the Sericulture & Entomology Experiment Station for Oriental garden crickets(Teleogryllus emma Ohmachi et Matsuura) and Modeagali-crickests(Loxoblemmus doenitzi Stein) collected from the lawns of Byeonsan-myeon, Buan-gun, Jeollabuk-do. The temperature conditions were 6 treatments from 15°C to 40°C at 5°C intervals using a multi-incubator, and the development period and mortality rate by age were compared using the 28°C breeding room conditions as a control. For T. emma, the higher the temperature, the shorter the total development period was, and the same trend was observed for the growth period by age stage. However, at low temperatures of 15°C and 20°C, all died after the third instar, and at high temperatures above 35°C, all died during development. At 25°C and 30°C, they developed normally, and the mortality rate was about 25%. The L. doenizi had the same tendency as the T. emma, with the higher the temperature, the shorter the development period. The total development period was 57.7 days at 25°C, which was similar to the T. emma's 55.9 days, and the mortality rate was the lowest at 44% at 25°C.
In 2022, research for native prokaryotic species in Korea reported 10 unrecorded bacterial strains affiliated to phyla Actinomycetota, Bacillota, and Pseudomonadota. The strains formed monophyletic clades with the most closely related species (with ≥98.7% sequence similarity) in the 16S rRNA gene sequencing. Among them, four species of the phylum Actinomycetota, two species of the phylum Bacillota, and four species of the phylum Pseudomonadota have not been reported in Korea, suggesting unrecorded species in Korea. Information on strains such as Gram staining reaction, colony and cell morphology, biochemical characteristics, and isolation sources were provided in the species description.
High-entropy alloys (HEAs) are attracting attention because of their excellent properties and functions; however, they are relatively expensive compared with commercial alloys. Therefore, various efforts have been made to reduce the cost of raw materials. In this study, MIM is attempted using coarse equiatomic CoCrFeMnNi HEA powders. The mixing ratio (powder:binder) for HEA feedstock preparation is explored using torque rheometer. The block-shaped green parts are fabricated through a metal injection molding process using feedstock. The thermal debinding conditions are explored by thermogravimetric analysis, and solvent and thermal debinding are performed. It is densified under various sintering conditions considering the melting point of the HEA. The final product, which contains a small amount of non-FCC phase, is manufactured at a sintering temperature of 1250oC.