Insect antennae play important roles in finding mates and in locating food source and oviposition sites. Riptortus pedestris is an important pest of soybean and sweet persimmon in Korea. The male R. pedestris adult produce the aggregation pheromone attracting the conspecific nymphs and both sexes of adults. The pheromone was known as a cue for food finding, but the 1st instar nymph can develop to the 2nd instar without food. This phenomenon may suggest that the 1st instar nymph may have different sensilla system from other instars. Thus, we investigated the morphology and distribution of antennal sensilla and antennal response to the aggregation pheromone (AG) of each nymphal stage using Scanning Electron Microscope (SEM) and Electroantennography (EAG). As expected, first instar nymph did not have sensilla trichodea 3 (T3) and chaetica 3 (Ch3) which existed in other instar nymphs. The antennae of the 1st instar nymph did not responded to AG, with no difference from control. For further elucidation of the functions of sensilla T3 and CH3, single sensillum recording to AG will be done.

1990년대 후반 IMO 해양사고 조사 결과(A.21/884-9)에 따르면 해양사고 중 75% 이상이 Human Elements와 인과관계를 가지는 것으로 보고된다. 또한 2010 마닐라 회의에서 결의된 문서, STCW Code 개정안에 따르면 안전항해 당직 유지를 위하여 항해사의 선교자원관리(BRM, Bridge Resource Management) 지식 및 자격에 관한 요건에 관한 내용을 강화하고 있다. 본 연구는 인적 요인에 의한 해양사고

1'0 determine Lhe ll1echanism of cell c1eath incluced by iron chelators. we explored the pathways of the three structurally relatecl ll1 itogen-activatecl protein(MAP) kinase subfami li esduring iron cbelator- inclucecl apoptosis ancl differentiation of oral precancerous ancl cancel‘ cells. The iron chelator c1 eferoxamine(DFO) exertecl potent timeancl c1ose-c1epenclent inhi bitory effects on the growth of IHOK and HN4 cells The major mechanism of growth inhibition following DFO treatment was fOllncl to be apoptosis incluction. as assessecl by annexin V-FITC staining. cell cycle analysis‘ DNA lacldering, a ncl Hoechst staining. We report that DF'O s trongly activates the p38 MAP kinase and extracell ular signal- regu lated kinase(ERK). but c10es not activate the c-Jun N-terminal kin ase/ stl않s-activaLecl protein kinase(JNK/8APK) . Of the three MAP kinase blockers usecl‘ the selective p38 MAP kinase inhibitor 8ß203580 ancl ERK inhibitor PD98059 protected oral premaIignant ancl malignant cells againsL iron chelator- nclllced cell death. which incl icates that the p38 MAP kinase serves as a major mecliator 01' apoptos is induced by this iron chelator DFO also evoked the release of cytochrome c from mitochondria, and incluced the activation of caspase-3 ancl caspase-8 in oral cancer cells, which suggests that apoptosis occurs via the mi tochoncl ri on - mecl iaLed pathway. DFO enhanced the expression of Bax in IHOK ancl HN4 cells. consistent witll thei r p53 status Moreover. DFO downregulatecl the expression 01' Bcl-2 in oral cancer cells. which suggests that DFO- incluced apoptos is 01' oraJ cancer and precancerous cells may be mediatecl by an increase in the ratio of pro-apoptotic to anti-apoptotic proteins. ln terestingJy, trcatmcnt 01' IHOK ancl HN4 cel ls with 8B203580 abolishecl cytochrome c release‘ as wel l as the activation of caspase-3 and caspase-8. DFO suppressecl the expression of epithelial di ffe rentiation markers, such as involucrin, t ransglutaminase II. CK6. and CK19. ancl this suppression was blockecl by p38 ancl ERK MAP kinase ll1hlbltors The oral premalignant(IHOK) ancl malignant cell s(I-lN4) showed differential responses to DFO with respect to the expression of cel l cycle regulatory proteins. cell growth. ancl apoptosis. Coll ectively. the current study reveals that p38 MAP kinase plays an ill1 portant role in iron chela tor-mecliatecl cel l cleath and in the suppression of differentiation of oral premalignantandmalignanLcell s.by activating a c10wnstream apoptotic cascade that executes the ceIl c1eath pathway

뻐ny studìes have shown the anti-proli ferative effects of irondeprivation on cancer cell s‘ but the effects 01' iron-chelators on oral cancer have not been clearly elucidated , To investigate the effects of an iron chelato r, desferrioxamine( 01"O).on the growth of ilIllTIortali zed human o1'al ke ratinocytes(IHOK), primary oraJ cancer cel ls(HN4)‘ metastatic oral cancer cell s(HN12) , and human skin keratìnocytes(HaCaT) in the MTr assay, three-dimensional(3D) raft cul tmes, Western blott ing, cell cycJ e analysis‘ nuclear staining‘ and cytochrome c expression for apoptosis s ig naling pathway were used OFO inhibited the growth of immortalized IHOK and HaCaT and mal ignant HN4 and HN12 keratinocytes in a time- and dose-dependent manner according to the MTT assay, The 3D organotypic cu l tu re also revealed that OF'O-treated cells showed less epithelial maturation, less surface keratinizati on‘ and de creased epithelia l thickness, The major mechanìsm of growth inhìbition with the micromolar 0 1"0 treatment was by the induction of apoptosis‘ which was supported by nuclear OAPI staining, ONA fragmentation analysis, and J10w cytometric analysis for sub-Gl phase ar rest and Annexin V-1"ITC stainìng, Furthermore‘ Bax expression in creased together with p53 and p21WAF1!CIPl, whìle the Bcl-2 expression decreased in the immortalized and malig nant keratinocytes treated with 01"0 , Time-dependent cytochrome c from mitochondria was observed in D1"O-treated [l-IOK and 0 1'머 cancel‘ ceJJ s, and was accompanied by the activation of caspase-3 in IHOK cells. These resu lts demonstrate that 0 1"0 has growth inhibitory effects on immortalized and malignant oral keratinocyLes Lhrough the induction of apoptosis and suggest that further evaluation of OFO as a potcntial thcrapcutic agent for human oral precancerous and cancerous lesions is warranted

Background : This study was conducted to developed the propagation method by cutting for mass cultivation of Vitex roundifolia. We were pitched the cutting two times and treated plant growth regulators to enhance the rooting percentage. Vitex roundifolia is live in beach sandy soil south of Hwanghae-Do and Gangwon-Do. Vitex roundifolia have been used to bath foam. It is good for aromatic plant. It has 0.8% essential oil content in leaf and flower. Major components of essential oil were alpha-Pinene, Sabinene, beta-Pinen, 1,8-cineole, d-Limone. Despite the superior usability, it had not yet been made by the artificial cultivation Methods and Results : We were pitched the cutting of a first-year branch on June 5, which was greenwood cutting and July 17, which was semi-hardwood cutting at Kwangseung-ri beach, Gochanggun, Jeonbuk. The length of cutting was 10cm. It had 3～4 nodes, we stuck a cutting remain 2 nodes above ground on ordinary raise seedling soil. Rooting percentage was measured at 60 days after stuck a cutting. Rooting percentage was higher greenwoody cutting(95%) than semi-hardwood cutting(57.6%). In green wood cutting, there was no significance with plant growth, but chemical injury was occurred in IBA 5,000ppm. In semi-hardwood cutting, there was significance with plant growth regulators. The rooting percentages of all the treat were higher than control(no treatment). Rooting percentage was the highest in NAA 5,000ppm treated. Conclusion : Greenwood cutting method was more proper to propagation for Vitex roundifolia than semi-hardwood cutting. The optimum time to cutting for Vitex roundifolia propagation was the early in June. If miss a time to propagation Rooting percent was elevated by plant growth regulator.

‘전미’는 2009년에 농촌진흥청 바이오에너지작물센터에서 전분용 고구마로 개발되었다. 이 품종은 ‘진홍미’를 모본으로 하고, 99IT55-2를 부본으로 하여 선발되었다. 2001부터 2003 년까지 계통선발시험과 2004년부터 2005년까지 2년 동안 생 산력검정시험을 수행하였고, 2006, 2008, 2009년 3년간 무안 등 전국 6개 도에서 지역적응시험을 수행하였다. ‘전미’의 엽 형은 심장형이고, 줄기 및 잎자루색은 녹색이다. 고구마 모양 은 방추형으로 표피색은 자색, 육색은 담황색이다. 선충 및 덩 굴쪼김병 저항성은 ‘중강’이며, 검은썩음병과 연부병도 ‘중강’ 이다. 전분가는 25.6%로 ‘율미’보다 높고, 전분수량은 6.8 t/ha 으로 ‘율미’ 보다 26% 높다. 총당 함량은 ‘전미’가 3.59 g/100 g, dry weight으로 ‘율미’에 비하여 20%가 높다. 당 종류별로 는 sucrose가 2.53 g/100g, dry weight으로 가장 많이 함유되 어 있다. ‘전미’의 조기재배 수량은 3개년 평균 26.1 t/ha으로 ‘율미’에 비하여 147%로 수량이 증수되었고, 만기 및 적기재 배는 26.2 t/ha으로 ‘율미’ 대비 139%로 수량이 높다. 조기재 배의 경우 주당 상품괴근수는 ‘전미’가 3.0개/주로 ‘율미’와 동일하였고, 상품괴근평균중은 151 g/개로 ‘율미’보다 12% 높다. 또한 만기 및 적기재배의 경우 주당 상품괴근수는 평균 3.0개/주로 ‘율미’ 2.3개/주개보다 많고, 상품괴근평균중은 178 g/개로 ‘율미’ 보다 높다.

본 연구는 국내 수집 잡초성벼에 존재하는 잎도열병 저항성 관련 유전자를 탐색하고, 이 저항성유전자와 연관된 분자마커를 탐색하는 것이다. 도열병에 감수성인 자포니카 품종인 낙동벼와 도열병에 강한 잡초성벼인 강화앵미11을 교잡하여 120개 RILs를 육성하여, 도열병 균주반응과 잎도열병 밭못자리검정을 통한 저항성 유전자 탐색에 이용하였다. 1. 총 45개 도열병 균주를 이용하여 양친들을 검정한 결과, 잡초성벼 강화앵미11은 25개 균주에 대하여 저항성 반응

‘청해진미’는 질소 소비료 적응 고품질 품종을 육성할 목적으로 1995년 하계에 국립식량과학원 답작과 벼품종개발연구팀에서 소비료 적응 특성을 가진 삼지연을 모본으로 병해충저항성 계통 SR14694-57-4-2-1-3-2-2와 양조특성이 우수한 이리 402호를 부본으로 3원 교잡하여 계통육종법에 따라 우량계통을 육성하였다. 선발된 우량계통에 대해 2005~2006년 2년간 생산력 검정시험 결과, 중만생이고 도정 특성과 쌀 외관 및 밥맛이 양호한 SR216