산화적 스트레스는 활성산소의 과도한 생성으로 인해 발생하며, 이는 만성퇴행성질환의 원인으로 알려져 있다. 본 연구에서는 식용 덖음 꽃차 8종 추출물의 폴리페놀 및 플라보노이드 함량, radical 소거 활성 측정을 통해 이들의 항산화 활성을 확인하고자 하였다. 8종의 식용 꽃차 에탄올 추출물 중에서 생강나무(Lindera obtusiloba, LO), 산수유(Cornus officinalis, CO) 및 카모마일(Matrecaria chamomilla) 꽃차 추출물의 총 폴리페놀 함량은 각각 20.21, 13.39, 12.39 mg gallic acid equivalent/g의 수치를 나타내었으며, 총 플라보노이드 함량은 LO, CO, 및 매화(Prunus mume) 꽃차 순으로 높은 수치를 나타내었다. 8종의 식용 꽃차 에탄올 추출물은 10, 25, 50, 100 μg/mL의 농도에서 2,2-diphenyl-1-picrylhydrazyl (DPPH) 및 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) 라디칼 소거 활성을 농도 의존적으로 증가시켰다. 특히, LO와 CO 꽃차 추출물은 다른 꽃차 추출물에 비해 우수한 DPPH 라디칼 소거 활성을 보였다. 또한 100 μg/mL 농도에서 CO, LO, 및 목련(Magnolia kobus) 꽃차 추출물의 ABTS+ 라디칼 소거 활성이 70% 이상의 수치를 나타내어 우수한 ABTS+ radical 소거 활성을 나타내었다. 본 연구 결과를 통해 식용 덖음 꽃차는 radical 소거 활성 및 폴리페놀과 플라보노이드를 함유함에 따라 항산화 활성을 나타냄을 알 수 있었으며, 특히 다른 꽃차 추출물에 비해 LO와 CO의 폴리페놀과 플라보노이드 함량 및 radical 소거능이 우수하여 항산화 기능성 소재로 활용될 수 있을 것으로 사료된다.

국립원예특작과학원에서는 밝은 화색과 안정적인 화형의 생 육이 우수한 빨간색 스탠다드 장미 품종을 육성하기 위해 진한 적색 스탠다드 장미 품종 ‘엔드리스러브(Endless Love)’를 모 본으로, 꽃잎수가 많고 안정적으로 가시가 적은 밝은 노란색 ‘페니레인(Penny Lane)’ 품종을 부본으로 인공교배하였다. 37 개의 교배실생을 양성해 1, 2, 3차에 걸친 특성검정 및 현장실증 을 통해 꽃이 크고 화형이 안정적이며, 재배안정성 및 생산성, 절화특성이 우수한 ‘원교 D1-390’을 최종 선발하였다. 2023년 ‘루비레드(Ruby Red)’로 명명하여 국립종자원에 품종보호출원·등록되었다. ‘루비레드’ 품종은 밝은 적색(R53C)을 가졌으 며, 꽃잎수가 32.8매, 화폭과 화고는 각각 10.9, 5.9cm로 대조 품종보다 크다. 절화장은 평균 71.7cm, 절화수명은 약 16.7일, 수량은 연간 168대/m2로 대조품종인 ‘레드스퀘어(Red Square)’ 대비 절화장이 길고 절화수명도 2배 이상 길며, 수확량도 1.4배 우수하다. 2023년 국내 육성 장미 품종 서울식물원 관람객 대상 공동평가회에서 스탠다드 장미 중 우수한 평가를 받았으며, 현 장 실증 결과 농가별로 균일하고 우수한 수량과 절화품질을 보 였다. 절화용 장미 ‘루비레드’ 품종은 밝은 적색과 우수한 화형 을 가지는 품종으로 해외 대체 품종으로 국내에서 많이 재배될 것으로 기대된다.

Sweetpotato (Ipomoea batatas L.) is a globally important food crop that is susceptible to infestation with the root-knot nematode, Meloidogyne incognita, which causes substantial crop losses. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to root-knot nematode (RKN) infection. As a result of previous study, RKN infection was confirmed in the RKN-susceptible sweetpotato cultivar Yulmi. Transcriptome analysis confirmed that among the genes that respond in this process, there are many genes related to ethylene biosynthesis. Therefore, in this study, we focused on the ACC oxidase (ACO) gene, the final enzyme of ethylene biosynthesis, and analyzed the expression patterns under various abiotic stress conditions. Using transcriptome data from our previous study, various expression changes in the four ACO genes used in this study were confirmed during RKN infection. The expression of G25011|TU41034 decreased during RKN infection compared to the untreated control, while the expression of G31097|TU51009, G28360|TU46486, and G15447|TU25395 genes increased in the early stages of RKN infection. Expressions of four ACO genes in leaves of sweetpoato were investigated under abiotic stress conditions such as wounding, high salinity, dehydration, and low temperature stress treatment. Expression of the G25011|TU41034 was significantly increased under abiotic stress conditions except low temperature. G31097|TU51009 was hardly expressed under abiotic stress conditions. Although the expression pattern of G28360|TU46486 and G15447|TU25395 was slightly different depending on the type of abiotic stress, an overall increase in expression was observed. It is expected that this study will be used as basic data on how ethylene biosynthesis responds not only to nematode infection but also to various abiotic stress conditions and will be helpful in functional studies of various crops.

The clearwing moth, Synanthedon bicingulata (Staudinger, 1887), is a pest that infests various species of cherry trees. However, genetic information regarding the genus Synanthedon including S. bicingulata, is limited. In this study, we sequenced a complete mitochondrial genome (mitogenome) of the species. The 16,255 bp of S. bicingulata mitogenome differs from the typical gene arrangement formed in Lepidoptera: trnQ-trnS2-trnM-trnI arrangement between the A+T-rich region and the ND2 junction. Moreover, the genome has untranslated repetitive sequences in the intergenic space between lrRNA and trnV, as well as the CGA start codon in COI and the TTG start codon in ATP8. Similar observations are noted in species belonging to the tribe Synanthedonini within the genus Synanthedon.

Since rice is the main food in Korea, there are no regulations on corn milling yet. Corn is known as one of the world's top three food crops along with wheat and rice, and it is known that 3.5 billion people worldwide use corn for food. In addition, corn mills are not developed or sold in Korea, but the use of corn mills is increasing significantly in many countries in Southeast Asia. In the Philippines, as Korea's rice mill import increases, Korea's KAMICO (Korea Agricultural Machinery Industry Cooperative) and domestic company A agreed to develop a corn mill jointly with PHilMech, an organization affiliated with the Philippine Ministry of Agriculture. However, research on corn milling was very insignificant, so the development was carried out based on the technology of Korea's rice mill. Rice milling is performed by peeling off the skin of rice and producing brown or white rice, so it is carried out by removing the skin and cutting the skin. On the other hand, in the corn mill, the skin of the corn is peeled, pulverized and selected to produce main products suitable for edible use. Therefore, in order to develop a corn mill, processes such as peeling, transfer, grinding, sorting, and by-product separation are required, and suitable parts must be developed. In addition, the performance must be gradually improved through experiments in which corn is repeatedly milled. The Philippines produces 7.98 million tons/year of corn, which is about 100 times that of Korea, and is mostly consumed as a staple food. This is about 10% of the total crop production in the Philippines. In addition, the main cultivation complexes of corn are the mountainous regions of Tarlac or Pangasinan, and the produced corn is 72.4% of the so-called yellow corn called Arabel and Sarangani, and the remaining 27.6% are known as white corn. In this study, it was intended to produce grains of 2.5 mm or less suitable for food for yellow corn and to develop a corn mill for 200 kg per hour. Detailed conditions for development are stipulated as more than 55% of the main product recovery rate, more than 31% of the by-product recovery rate, less than 5% of the raw material loss rate, and more than 80% of the embryo dislocation rate. In this study, to achieve this, the overall process of the corn mill was developed, and the optimal conditions for the corn mill were obtained through the development of parts and empirical tests to improve performance. In addition, it was intended to achieve the development goal by evaluating and analyzing the performance of each part so that it did not conflict.

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

우리나라 정원의 식물 이용 현황을 알아보고자 전국 36개 정원을 대상으로 정원식물을 조사하였다. 정원은 6가지 유형 으로 구분하였다. 그 결과, 36개소 정원에는 정원식물 712종 류가 식재되고 있었다. 교목, 관목, 초본의 비중은 평균 1:2:7 이었다. 개별 정원(10×10㎡)에서 교목은 평균 2.5종으로 12.6%, 관목은 4.1종 20.7%, 초본은 13.2종 66.7%를 차지했다. 식재 빈도 상위 5%에 40여 종이 포함되었으며, 맥문동, 일본조팝나 무, 수국, 회양목, 단풍나무, 옥잠화, 에키네시아, 산철쭉이 대 표적이었다. 양지형 정원에는 관목이 상대적으로 적고 초본이 많았으며 다른 유형보다 20~40% 이상 다양했다. 그늘형은 초 본보다 교목이 많았으며, 건조형 및 습지형과 마찬가지로 식 재 다양성이 20% 감소했다. 옥상정원의 도시형 정원은 초본 이 전체의 80~90%를 차지하고, 해안형 정원에는 목본과 초본 이 1:1로 목본 중심의 식재 특성을 보였다. 결과적으로 정원 유형에 따라 식재 다양성과 구성 비율이 명확한 차이를 나타 냈다.

A pregnancy diagnosis is an important standard for control of livestock’s reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows (“open” cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy (“open” cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.

In this study, the effects of heat-moisture treatment (boiling or steaming for 45 min) prior to hot air drying (70 o C for 8 h) on water activity (Aw), moisture (MC), Brix, color, and texture of dried sweet potato slices were investigated to identify the best pretreatment condition for producing dried sweet potato with the best eating quality. Dried sweet potato slices pretreated by boiling (BL) showed significantly lower Aw, MC, and hardness while Brix was significantly higher than with steaming (ST) treatment. There were no significant differences for the color, springiness, cohesiveness, gumminess, and brittleness indexes. At 8 h drying, the Aw, MC, Brix, and hardness of the BL and ST samples were 0.81 and 0.82, 19.71% and 25.53%, 53.80% and 49.40%, and 20.49 kg/cm 2 and 31.98 kg/ cm 2 , respectively. This information will be useful for manufacturers in the production of dried sweet potato slices. These findings provide evidence of the feasibility of heat-moisture treatments in the production of dried sweet potato.

본 연구는 제비동자꽃의 대량번식을 위한 효과적인 발아 조건을 선발하고자 수행되었다. 제비동자꽃 종자는 저온습윤 처리(5°C, 0, 4, 8, 12, 16주), GA3 처리(0, 500, 1000mg･L-1), 프라이밍 처리(0.1M, 0.2M의 NH4NO3, K2HPO4, KCl, KNO3)를 하였다. 모든 종자는 25°C, 12시간 광조건(40μmol･m-2 ･s-1)에 서 발아 실험이 진행되었으며, GA3 처리구는 비닐온실(평균 23.4°C)에서도 비교실험이 진행되었다. 저온습윤 8주 처리구 에서 20.4%로 가장 높은 발아율을 나타냈으며, 12주 이상의 저온습윤 처리는 오히려 제비동자꽃의 발아율을 감소시켰다. GA3 0, 500, 1000mg･L-1 처리된 종자는 25°C에서 각각 3.3, 44.0, 62.0%, 비닐온실에서 각각 5.3, 16.7, 42% 발아하였다. 제비동자꽃 종자는 GA3 농도가 높아질수록 발아율이 증가하였다. 또한 제비동자꽃 종자는 0.2M KNO3-프라이밍 처리구에서 가장 높은 발아율(54.7%)을 나타냈으며, 평균발아소요일수는 처리 간 유의성을 보이지 않았다. 이상의 결과로 효과적인 제비종자꽃 종자 번식을 위한 전처리 조건으로 24시간 GA3 1000mg･L-1 침지 처리 또는 0.2M-KNO3 프라이밍 처리를 추천한다.

This article reports on a classroom-based study that examined the effects of direct focused and unfocused written corrective feedback (WCF) on the accuracy development of the past hypothetical conditional (e.g., If I had had money, I would have bought a new computer.) and the indefinite article (a/an). The study employed a pretest-posttest-delayed posttest design with two feedback treatment sessions. Six intermediate classes, a total of 108 college freshmen at a university in Korea and two native English speaking instructors, participated in the study. The classes were assigned into a focused WCF group, an unfocused WCF group, and a control group. A text-reconstruction task was used as a writing material and a test instrument. The results revealed that both focused and unfocused WCF groups outperformed the control group in the accuracy gains of both target forms. When the relative effects of two types of feedback were considered, there was no difference in the contribution focused and unfocused WCF made for the accuracy improvement of the hypothetical conditional. However, focused WCF was more beneficial than unfocused WCF for the development of the indefinite article.

83세 여자 환자가 신장결석으로 입원하여 금식, 세프트리악손 투여 및 경피적 신쇄석술 후 퇴원하였고, 이후 명치 통증이 발생하여 응급실로 내원하였다. 혈액검사 및 복부 전산화 단층촬영에서 담석 및 췌장염 소견을 확인하였고, 담석은 이전에 보이지 않던 소견이었다. 이에 세프트리악손 유발 담석에 의한 급성 췌장염으로 진단하였고 보존적 치료 및 담낭절제술 후 퇴원한 예를 경험하여 보고하는 바이다.

In this study, commercial activated carbons (ACs) were upgraded by different activation methods, and the gases generated during the activations were defined and quantified. The chemical activation commonly applied for upgrading ACs uses complex reactions, involving pyrolysis, physical, and chemical reactions. The ACs based on wood materials were characterized by elemental analysis, N2 physisorption, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and temperature-programmed desorption mass spectrometry. The patterns and composition of the generated gases were analyzed by gas chromatography and X-ray diffraction; high-resolution scanning electron microscopy was also used to characterize the activated carbon. The AC was mostly decomposed to CO2 by pyrolysis and physical activation, while CO was mainly detected during chemical activation from the K2CO3 produced by the reactions between CO2 and K2O. The detected amounts of generated gases were differed at various KOH ratios and residence times. The highest surface area obtained in this study was 2000 m2/g at the optimum ratio of AC and KOH (1:2).

This study investigated the effects of negotiation in the process of providing written corrective feedback (WCF) by comparing three different patterns: non-negotiation, minimal negotiation, and extended negotiation. The data were collected from three intermediate academic English classes at a university in Korea. Each class was exposed to different degrees of negotiation while they received WCF for two target forms, the past verb forms and the English articles, in their written production (text re-construction tasks). Their accuracy improvement was measured by text-reconstruction writing tasks and error correction tests. The study found that the use of negotiation affected accuracy improvement of the target forms; however, its effectiveness varied depending on the target forms and measurements. For the past tense, negotiation, regardless of its amount, resulted in more accuracy improvement than non-negotiation in both text re-construction tasks and error correction tests. However, for the English articles, negotiation operated differently: extended negotiation significantly brought about more accuracy development in both measurements, but the difference between minimal negotiation and non-negotiation was only observed in the error correction test, not in the text re-construction task.

목적: 이 연구는 안경과 선글라스에서 테와 렌즈의 형태와 색상에 대한 한국인과 중국인 소비자 집단의 선호도 차이를 조사하기 위하여 실시되었다. 방법: 한국에 유학 중인 중국인 학생 150명과 한국인 대학생 150명을 대상으로 설문조사를 실시하고 그 결과를 분석하였다. 안경광학 관련 전공자는 설문 대상에서 배제되었다. 중국인 학생들에게는 중국어 간체자로 번역된 설문지를 제공하였으며, 제공된 설문지는 한국 대학 석사과정에 유학 중인 중국인 원어민 학생이 한국어 설문지를 바탕으로 번역한 것이다. 결과: 중국인 소비자 집단은 안경보다 선글라스에서 글로벌 유명 브랜드를 더 선호하였으며, 한국산 안경 제품을 상대적으로 선호하는 것으로 나타났다. 또한, 무테 또는 반무테, 금색테, 투톤컬러 렌즈 항목에서 한국인 소비자 집단보다 높은 선호도를 나타냈다. 결론: 이 연구의 결과는 한국 안경 제조․유통 업체의 중국 시장 진출이나 중국인 고객 비율이 높은 안경원 의 판매 전략 수립에 기여할 수 있을 것으로 기대된다.