본 연구는 자일로올리고당의 과학적이고 체계적인 표준 화된 시험법을 마련하여 다양한 제형의 제품에 적용하고 자 하였다. 최적화된 시험법을 마련하기 위해 초음파 처 리 시간, 산 가수분해 시간 및 농도를 검토하여 전처리 방 법을 비교 평가하였으며, HPLC-UVD를 이용하여 시료 중 의 자일로올리고당을 분석하였다. 분석 시 1-phenyl-3- methyl-5-pyrazoline (PMP)으로 유도체화하고, photo diode array (PDA)가 장착된 high performance liquid chromatography (HPLC) (Nanospace SI-2)를 사용하였으며, 칼럼은 Cadenza C18 (4.6 × 250 mm, 3 μm)이었으며, 이동상은 pH를 6.0으 로 맞춘 20 mM 인산완충용액과 아세토니트릴을 78:22 비 율로 혼합하여 사용하였고, 0.5 mL/min 유속으로 254 nm 로 하여 분석하였다. 건강기능식품 등 시험법 마련 표준 절차에 관한 가이드라인에 따라 밸리데이션을 수행하고, 표준화된 시험법을 이용하여 유통 중인 건강기능식품 대 상 품목에 대해 시험법 적용 여부를 확인하였다. 표준화 된 시험법은 자일로올리고당을 함유한 건강기능식품 품질 관리에 대한 신뢰성을 더 높일 것으로 본다.

본 연구는 그린커피빈추출물이 「건강기능식품의 기준 및 규격」에 추가로 등재될 경우를 대비하여 표준화된 클 로로겐산 시험법을 설정하고, 카페인이 동시 분석되도록 최적화하는 연구를 진행하였다. 최적화된 시험법을 마련 하기 위해 기기분석 및 전처리 조건을 비교·분석하여 클 로로겐산과 카페인을 30% 메탄올 추출하여 인산용액과 인산 함유 아세토니트릴으로 액체크로마토그래프를 통해 330 nm, 280 nm에서 분석하도록 시험법을 설정하였다. 시 험법 밸리데이션 결과, 직선성 정량범위 내에서 상관계수 (R2) 0.999 이상의 유의수준을 보였고, 클로로겐산과 카페 인 검출한계는 0.5와 0.2 μg/mL, 정량한계는 1.4와 0.4 μg/ mL로 나타났다. 정밀도와 정확도 결과는 AOAC 밸리데 이션 가이드라인를 통해 적합함을 확인하였고, 클로로겐 산 및 카페인 동시분석법을 최종적으로 마련하였다. 또한, 시제품과 유통제품을 통해 제형별 적용성 검토하여 클 로로겐산과 카페인을 동시에 정량 가능한 시험법임을 재확인하였다. 최적화된 시험법은 클로로겐산을 함유한 건강기능식품 품질관리에 대한 신뢰성을 더 높일 것으 로 본다.

Tube, an intracellular protein of the Toll-pathway, forms a complex with Pelle and MyD88, and regulates a signal transduction to activate NF-κB in Drosophila. To understand the antimicrobial function of TmTube, the induction patterns of TmTube were investigated at 3, 6, 9, 12, and 24 h-post injection of pathogens into 10th to 12th instar larvae. In addition, we investigated the effects of TmTube RNAi on larval mortality and tissue specific AMP expression in response to microbial challenge. Our results will provide a basic information to elucidate the immunological function of TmTube

Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of glycosidic bonds in crystalline polysaccharides including chitin and cellulose. The recent discovery of LPMO family proteins in many insect species suggests that they presumably play a role in chitin degradation in the cuticle/exoskeleton, tracheae and peritrophic matrix during insect development. Insect LPMOs belong to auxiliary activity family 15 (AA15/LPMO15) and have been classified into at least four groups based on phylogenetic analysis. In this study, we identified and investigated the physiological functions of group I LPMO15 (MaLPMO15-1 and PhLPMO15-1) in two longhorn beetle species, Monochamus alternatus and Psacothea hilaris. In both species, depletion of LPMO15-1 transcripts by RNAi resulted in a lethal pupal-adult molting defect. The insects were unable to shed their old pupal cuticle and died entrapped in their exuviae. Furthermore, TEM analysis revealed a failure of degradation of the chitinous procuticle layer of their old cuticle, retaining intact horizontal laminae and vertical pore canals containing perpendicularly oriented chitin fibers (pore canal fiber, PCF) in their core. These results indicate that MaLPMO15-1 and PhLPMO15-1 are required for turnover of the chitinous old cuticle, which is critical for insect molting.

The genus Argiope are known to decorate their webs with various patterns. The purpose of these web decorations, referred to as stabilimenta, is still a subject of debate. They are believed to serve either as lures to attract prey insects or as a means of providing protection to the spider by enhancing the web's visibility to deter potential predators. In this study, we observed spider orientation with respect to the direction of the sun in a controlled laboratory environment. To assess the orientation preference of the spider, the frame was repositioned daily by reversing its direction. Over a period of 21 days, the orientation of the spider was meticulously recorded, distinguishing between the illuminated side (open side) and the shaded side (closed side). field emission scanning electron microscopy (FESEM) visualization were used to investigate the microstructural characterization of stabilimenta in both juvenile and adult spiders. Our results represent that the decorative structure of stabilimentum shows diverse microstructural features according to the maturation level of the spider. In addition, it has been confirmed that spiders tend to prefer the open side direction when stabilimentum is present.

In the Gucheon stream of Deogyusan National Park, a survey was conducted at three sites (six sites in total) in 2018 and 2021. A total of benthic macroinvertebrates of 107 taxa 46 families 13 orders in 6 classes of 5 phyla were found. According to the analysis of the appearance by year, a total of 87 species were surveyed in 2018, and a total of 89 species were surveyed in 2021. As a results to the functional feeding group analysis, Gathering-Collector (GC) accounted for a high species share (2018: 29.89%, 2021: 25.84%). In the functional habitat group analysis, Clinger (CL) accounted for a high species share (2018: 56.32%, 2021: 57.30%). Average annual community indices analysis, DI 0.38(±0.05), H′ 3.48(±0.19), R′ 3.23(±0.41), and J′ 0.85(±0.04) in 2018, and DI 0.55(±0.09), H′ 2.98(±0.31), R′ 2.91(±0.65), and J′ 0.70(±0.05) in 2021. As a results of the benthic macroinvertebrates index (BMI) analysis, indicating that all sites were classified as "very good," with average BMI values of 95.24(±2.84) in 2018 and 96.99(±1.13) in 2021.

최근 해사산업 분야에서는 4차 산업혁명의 대명사로서 자율운항선박 관련 자율운항기술 및 원격운항기술 등의 개발이 활발히 진행되어 자율운항선박에 대한 본격적인 실증이 현실화되고 있다. 이러한 자율운항선박이 현실화가 된다 면 이러한 선박이 국제해사협약 체계 내에서 아무 문제없이 운항이 가능해야 할 것이며, 이를 위한 정책적, 법제적 준비가 제대로 이루어져야 할 것이다. 이 를 위해 자율운항선박이 운용되기 위하여 반드시 고려되어야 하는 인적요인의 역할 변화에 대한 법제적 및 기능적 검토가 필요하다. 먼저 국제협약 및 국내법에서는 인적요인에 대한 여러 규정을 두고 있으며 선박의 해상에서의 안전을 확보하기 위하여 자격 있는 선원의 승선 및 통제를 전제로 하여 선박과 인적요인을 분리하고 있지 않다. 다만, 자율운항선박의 경 우 기국이 재량에 따라 판단하여 선원이 승선하지 않아도 안전하고 효율적으로 선박을 운항할 수 있다고 판단한다면 반드시 선원의 승선이 필요한 것은 아니 라고 볼 것이다. 특히, 선박의 안전운항을 위하여 그동안 선장 및 해기사가 수 행해 온 역할들이 누락없이 축소된 최소 선원, 원격운항자 및/또는 첨단기술로 적절히 이전된다면 그러한 시대변화를 뒷받침할 수 있을 것으로 보인다. 따라서 이 논문에서는 IMO에서 논의가 되고 있는 원격운항자의 역할과 책임 과 관련하여 기존 해기사의 기능 중 원격운항자가 이어 받아 수행할 수 있는 기능을 식별하고 그러한 기능에서 요구되는 해기능력을 확인하여 제시하고자 하였으며, 향후 원격운항자의 요구능력으로 참조할 수 있을 것이다.

The purpose of this study was to develop the Functional golf inner-wear by preventing the injuries and enhancing the performance of the Golf swing by checking the influence of the wearing of the functional golf inner-wear considering golf characteristics on the Swing trajectory and Shot distance. Functional inner-wear effective for golf swing was manufactured using the sports taping method. Changes in driver and iron swing before and after wearing the functional golf inner-wear manufactured in this way were measured using trackman equipment. Measurement variables were limited to Club Speed, Attack Angle, Club Path, Ball Speed, Smash Factor, and Priority. Before and after wearing functional golf inner-wear, there were statistically significant differences in driver club speed, iron club speed, driver etch angle, iron club pass, driver ball speed, driver smash factor, iron smash factor, driver carry, iron carry, and right shoulder joint proprioceptive sensory ability. As a result, functional golf inner-wear is effective for ball speed, impact, and carry by increasing club speed and efficient swing. Future research will focus on the development of functional golf that can improve the swing ability in a short game that plays an important role in the golf game through various sports taping grafting technique, textile, special material, film, Research on functional golf inner-wear.

In Korea, the function of Jeong(亭), Jeongja(亭子), and Nujeong(樓亭) architecture is essentially rest. However, in China, where the name Jeong was first coined and used, Jeong was not only used as a place of rest, but also for a variety of functions depending on the time and region, such as building units for guard posts, policing, and mail delivery. But why is it that in Korea, Jeong is primarily perceived and used as place of rest? Starting from this question, this article examines the historical process of the emergence of Jeong and its changing functions, focusing on the official history of China, and then examines the perception of Jeong in Korea through its connection to the dominant function of Jeong in China during the period of Jeong's emergence in Korean literature. This will help us to fundamentally understand the differences between Jeong architecture in Korea and Jeong architecture in China, and to further clarify the historical significance of the types of Jeongja and Nujeong architecture in Korea.

The global pet market is continuously growing as the number of single-person households increases along with the preponderance of the nuclear family, so pet-related industries are steadily growing. In addition, the market related to the health of companion animals is also increasing significantly. Regarding the health of companion animals, it is reported that the incidence of adult diseases, like humans, is growing mainly due to activities in indoor environments and the lack of exercise due to the lack of time for companion animals. This paper studies the antioxidant properties of Jerusalem Artichoke extract, which can be used as a raw material for functional foods related to adult diseases in companion animals by using substances extracted from Jerusalem Artichokes grown by the first author. Samples to be tested were extracted from hot water extraction and ethanol extraction methods according to conditions. To confirm the antioxidant properties, absorbance analysis, thin-layer chromatography (TLC) analysis, polyphenol measurement, flavonoid measurement, and radical scavenging ability measurement by DPPH were performed to determine whether the raw materials applied in this paper could be used. It was confirmed that Jerusalem Artichoke extract is a suitable additive raw material for functional pet food.

By developing molds and facilities to horizontally mold the functional part of the dry-cast concrete block, We intend to develop molds and a series of facilities to horizontally mold the functional part of the dry-cast concrete block to increase production per cycle while maintaining existing production methods and major facilities. In order to do so, CAE analysis is first required to develop molds and facilities for horizontally molding the functional part of the drycast concrete block in the horizontal direction. The procedure will be carried out by reviewing the validity of boundary conditions and physical properties, 3D modeling, grid generation, construction of analysis models, model validity, analysis according to frequency changes, and analysis according to physical properties. First, through the comparison of two-point support, three-point support, and two-point and three-point support in the constraint conditions, We would like to compare it with the actual molded product in the horizontal direction. But first of all, it is considered two-point support in the constraint conditions in this paper.

Background: Hemiparesis not only affects the affected side but also exerts an impact on the unaffected side. Stroke patients endure muscular weakness attributable to hemiparesis, resulting in asymmetry of muscular strength between the paralyzed and non-paralyzed sides. Objectives: To investigate the correlation between lower limb muscle strength asymmetry and functional factors in patients with stroke. Design: A cross-sectional study. Methods: A total of 58 stroke patients participated in this study. After measuring the muscle strength of both lower limbs using manual muscle testing, the asymmetry was calculated. Functional factors such as berg balance scale (BBS), timed up and go (TUG), 10-metre walk (10MW), and modified barthel index (MBI) were measured. Results: The BBS showed positive correlations with hip, knee, and ankle strength asymmetry (P<.05). The TUG showed negative correlations with hip, knee, and ankle strength asymmetry (P<.05). The 10MW showed negative correlations with hip, knee, and ankle strength asymmetry (P<.05). The MBI showed positive correlations only with hip strength asymmetry (P<.05). Conclusion: We were found that there is a more pronounced lower limb muscle strength asymmetry in the lower extremity of stroke patients, which is associated with BBS, TUG, and 10MW.

Background: Chronic back pain not only causes physical discomfort and decreased functionality but also affects emotional well-being, resulting in feelings of anxiety, depression, and other psychological issues. Studies have indicated that cognitive functional therapy and motor control exercises can alleviate chronic low back pain and associated psychological issues. Objectives: To investigated the effect of chronic low back pain on the cognitive functional therapy (CFT) and motor control exercises (MCE). Design: Quaxi-experimental study. Methods: Forty patients with chronic low back pain were randomly assigned and divided into 20 cognitive functional therapy group with motor control exercises group (CFTMG) and 20 patients in motor control exercises group (MCEG). After applying CFT with motor control exercises and motor control exercises for 8 weeks, changes in numeric pain rating scale (NPRS), Oswestry disability index (ODI) and Fear-avoidance beliefs questionnaire (FABQ) were observed. Results: In the CFTMG, statistically significant changes were observed in NPRS, ODI, and FABQ before and after the intervention. In the MCEG, statistically significant changes were observed in VAS and ODI before and after the intervention. Conclusion: The application of CFTM and MCE in patients suffering from chronic low back pain has demonstrated effectiveness in alleviating pain, enhancing back function, and reducing kinesiophobia associated with the condition.

Abstract Purpose : This study analyzed physical properties using 1,6-Hexanediol diacrylate (HDDA) as an additive to improve functionality of hydrogel lens such as durability. It was checked whether HDDA can be applied as a crosslinking agent in place of ethylene glycol dimethacrylate (EGDMA). Methods : Polymerization used thermal polymerization and photopolymerization methods, and HDDA was added to each polymerization method at a ratio of 1∼20%. Optical and physical characteristics of the manufactured lens were evaluated by measuring spectral transmittance, refractive index, water content, tensile strength, contact angle, and AFM. Results : Regardless of the polymerization method, the tensile strength ranged from 0.232~0.408 kgf/mm2 in thermal polymerization and from 0.146~0.429 kgf/mm2 depending on the addition ratio of HDDA. In addition, as a result of using HDDA instead of EGDMA, it was confirmed that the physical characteristics of the lens were similar to that of EGDMA, and in the case of tensile strength, HDDA was much improved. Conclusion : HDDA has been shown to be effective in improving the functionality of hydrogel lens and to improve stability and durability. In addition, it is believed that it can be used in various ways as an ophthalmic material as well as a photopolymerization crosslinking agent. Key words : Crosslinking agent, Tensile strength, 1,6-Hexanediol diacrylate, Hydrogel lens

The purpose of this study was to investigate nutritional and functional ingredients and antioxidant activity after fermenting rice bran using Lactobacillus brevis for 24, 36, and 48 hours. The results of the analysis of the nutritional ingredients revealed that there was no significant difference in the carbohydrate, crude protein, crude fat and ash content regardless of the fermentation process and fermentation time. The amount of dietary fiber was significantly different between the unfermented and fermented rice bran and was observed to be the highest after a 48-hour fermentation. The -oryzanol, gamma-aminobutyric acid (GABA) and total phenolic contents were significantly higher in the fermented rice bran compared to the unfermented rice bran (p<0.05) and the GABA and total phenolic contents increased significantly as the fermentation time increased (p<0.05). The 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6- sulfonic acid (ABTS), superoxide and hydroxyl radical scavenging activities used to measure antioxidant effects significantly increased as the fermentation time increased (p<0.05). From these results, it was confirmed that the antioxidant effect and functional components, namely -oryzanol, GABA, and the total phenolic content of rice bran improved with fermentation. Based on these results, fermented rice bran could be presented as a functional material for use in high value-added industries.

사회적 기업은 효율과 경쟁 중심의 자본주의 시장경제 속에서 발생하는 양극 화, 고령화 사회, 환경문제 등 다양한 사회 문제를 해결하기 위하여 등장하였 다. 특히, 세계적인 금융위기가 지속되면서 전통적인 사회적 기업인 협동조 합이 대안 모델로 주목받고 있다. 하지만, 이러한 협동조합이 자본주의 시장 경제 속에서 경쟁력을 갖춘 사회적 기업으로 지속가능할 것인가에 대해서는 다소 의문이 제기되고 있다. 그 이유는 협동조합이 사업체임과 동시에 사회 적 목적을 실현하는 단체로서의 이중성을 갖고 있으며, 주식회사와 다른 고 유한 조직적 특성이 있음에도 불구하고 이러한 특성이 제대로 반영되지 못하 고 있기 때문이라고 한다. 이에 이러한 특성을 반영하고 그 기능과 역할을 제대로 수행할 새로운 형태 의 사회적 기업을 요청하게 되었다. 따라서 본 논문은 사회적 목적과 가치를 실현하며 정부의 지원 없이도 독자적으로 지속 가능한 운영을 할 수 있는 새 로운 형태의 사회적 기업으로 미국의 L3C, 인증 B콥 기업, BC를 검토하고자 하였다. 이러한 제도는 유럽과 달리 국가 내지 정부 중심이 아닌 기업 중심으 로 기본적으로 영리추구형 사회적 기업이며, 독자적 형태의 존립 기반과 정 부의 지배를 받지 않는 자율적인 운영을 하는 기업이기 때문이다. 생각건대, 사회적 기업으로서 기능과 역할을 제대로 수행하기 위해서는 유럽식의 사회 적 기업 모델은 한계가 있다고 생각된다. 따라서 위에서 요청하는 역할과 기 능을 실질적이고 지속적으로 수행할 수 있는 미국의 새로운 형태인 사회적 기업에 대한 검토를 하고, 이를 도입할 경우 현행 법제도상 어떤 문제점을 검 토할 것인지에 대하여 살펴보았다.

This study aimed to prepare kombucha, a fermented tea beverage, containing Dendropanax morbiferus (DM) leaves and roots, and analyze its antioxidant and intracellular activities. We compared the pH change, total acidity, radical scavenging activity, and oxygen radical absorbance capacity (ORAC) of kombucha fermented with black tea alone and that with added DM leaves or roots during fermentation. Using RAW 264.7, we evaluated the effects of kombucha containing different DM parts on nitric oxide (NO) production and inflammation-related cytokine content in cells. Kombucha containing ethanol extracts of DM leaves (BTK-E-DML) and roots (BTK-E-DMR) showed higher radical scavenging activity and ORAC 3 d after fermentation than that prepared from black tea alone (BTK-Ori). In an in vitro experiment using RAW 264.7, samples were treated with 8 mg/mL kombucha considering cytotoxicity; the lipopolysaccharide (LPS)-induced NO content significantly reduced after BTK-E-DML and BTK-EDMR treatments compared with that after BTK-Ori treatment. Additionally, the levels of interleukin-6 and tumor necrosis factor-alpha, which were LPS-stimulated inflammatory cytokines, significantly decreased in cells treated with BTK-E-DML and BTK-E-DMR 15 d after fermentation compared with those treated with BTK-Ori. In conclusion, these results demonstrate that kombucha fermented with the leaves and roots of DM increases antioxidant activity and can significantly regulate inflammatory responses at the cellular level.

The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 μg/g. Lutein was detected at levels between 70.97 and 597.97 μg/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.