The purpose of this study is to execute the investigation of injurious factors causing muscle-skeletal diseases at an automotive glass manufacturing company and, on the basis of the results, to enhance productivity by the improvement of working environment. By using an survey and an ergonomic evaluation method, symptoms of muscle-skeletal diseases of workers and working postures at each process were analyzed, and quantitative evaluation of muscle-skeletal disease risk against each process was performed. Based on the result of the evaluation, to enhance the working environment, improvement of worktable, working space, tools, and outfit was suggested, and induction of mechanical system was also suggested. Suggested improvement plan was applied to the workplace step by step and it is confirmed that improvement plan not only removes the injurious factors of muscle-skeletal diseases but also is effective to enhance productivity.

In order to enhance the quality of the glass bottle and to preserve the contents long, corrosion of the bottle surface is often required. The corrosion operation consists of dangerous and harmful tasks using strong acid such as HF. Thus, the corrosion operation should be automatized to improve the worker's safety. The first step of the automation is to develop the bottle gripping system that automatically grips and fixes the bottle during corrosion operations. The gripping system consists of holders, holder plates, air cylinders, and bottle aligning system. This paper shows the gripping system development procedure.

The purpose of this study is to execute the investigation of injurious factors causing muscle-skeletal diseases at an automotive glass manufacturing company and, on the basis of the results, to enhance productivity by the improvement of working environment. By using an survey and an ergonomic evaluation method, symptoms of muscle-skeletal diseases of workers and working postures at each process were analyzed, and quantitative evaluation of muscle-skeletal disease risk against each process was performed. Based on the result of the evaluation, to enhance the working environment, improvement of worktable, working space, tools, and outfit was suggested, and induction of mechanical system was also suggested. Suggested improvement plan was applied to the workplace step by step and it is confirmed that improvement plan not only removes the injurious factors of muscle-skeletal diseases but also is effective to enhance productivity.

The German Werkbund, which was founded in 1907, played an important role in the history of modern architecture. Its exhibition 1914 in Cologne is estimated as a meaningful event in the development of modern architecture. Especially two examples, among which were built at that time, are worthy of notice. The one is the 'Modelfactory' by Walter Gropius and the other 'Glashaus' by Bruno Taut. Generally in the Textbook on the history of modern architecture, the Taut's Glashaus is rarely mentioned or described as a early example of some expressionistic architecture, while the Modelfactory by Gropius is regarded as an essential workpiece in the early stage of modern movement. the time of searching alternative not only from rationalistic modernism but also postmodernism and today in the time of plurality, Taut's Glashaus could bring us more interesting and meaningful aspects in architectural design. Through investigating the background in the planning stage and analysing the composition of space, construction, circulation etc. it is to try to understand the building as really as what it was. Furthermore, historical meanings of the building in the modern architecture is reinterpreted in the following aspects; what should be reflected in architecture and how could 'Zeitgeist' be architecturally adopted?

망사시험관 및 유리시험관에서 톱다리개미허리노린재의 약충발육, 성충수명 및 산란수를 비교하였다. 약충기간은 유리시험관(20일)보다 망사시험관(약 21일)에서 약 1일 길었는데, 이러한 차이는 주로 5령충의 발육기간에 기인되었다. 우화율은 유리시험관()보다 망사시험관()에서 높았다. 성충수명은 유리시험관보다 망사시험관에서 암컷이 약 4.1배, 수컷이 약 6배 길었다. 산란전기간은 유리시험관보다 망사시험관에서 약 1일 길었으며, 산란수는 유리시험관보다 망사시험관에서 약 3.5배 많았다. 산란지속기간은 망사 시험관에서 약 30일, 유리시험관에서 약 8일 이었다. 따라서 톱다리개미허리 노린재의 발육특성을 정확하게 구명하기 위해선 공기유통이 원활한 망사시험관을 사용하는 것이 적합할 것으로 판단되었다.

본 연구는 돼지 미성숙 난포란의 유리화 동결액에 노출시 독성과 삼투압 스트레스가 가장 적은 유리화 동결액을 조사하기 위하여 실시하였으며, 그 결과를 요약하면 다음과 같다. 미성숙 난포란을 EFS(40% ethylene glycol +18% ficoll +0.3 M sucrose) 용액, ES(5.5 M ethylene glycol +1.0 M sucrose) 용액, GE(10% glycerol +20% ethylene glycol) 용액에 각각 노출하고 대조구로 아무 처리하지 않은 난포란을 체외성숙하여 관찰한 체외성숙율은 ES 용액과 대조구 에서 EFS 용액과 GE 용액보다 유의적으로 높았으며(P<0.05), 유리화 동결액의 독성에 의한 난포란의 퇴화율은 대조구, ES 용액, EFS 용액, GE 용액 순으로 유의적으로 낮은 퇴화율을 나타내었다(P<0.05). 미성숙 난포란을 EFS 용액, ES용액, GE용액에 각각 노출하고 대조구로 아무 처리하지 않은 난포란을 체외성숙, 체외수정하여 관찰한 정상 수정율은 대조구가 세 종류의 유리화 동결액보다 유의적으로 높았고(P<0.05), 유리화 동결액 간에는 유의적인 차이가 없었다. 다정자 침입율과 난자당 침입한 평균 정자수는 모든 처리구간에 유의적인 차이가 없었다. 미성숙 난포란을 EFS 용액, ES 용액, GE 용액에 각각 노출하고 대조구로 아무 처리하지 않은 난포란을 체외성숙, 체외수정, 체외 배양하여 관찰한 수정 3 일째의 분할율은 ES 용액과 대조구가 EFS 용액과 GE 용액보다 유의적으로 높았으며(P<0.05), 수정 7일째의 배반포 형성율은 모든 처리구간에 유의적인 차이가 없었다. 배양기간 동안 나타난 퇴화율은 대조구가 세 가지 유리화 동결액에 비해 유의적으로 낮았고(P<0.05), ES 용액은 EFS 용액과 GE 용액보다 유의적으로 낮은 퇴화율을 나타내었다(P<0.05).

본 연구는 배지 구성물질로서 발포 유리질 소재(CGF)와 탄화 밤나무 목재입자(CCW)를 이용하여 배지를 재발하고 원예재배용 배지의 국산화를 위해 수행되었다. 토마토 종자는 발포 유리질 소재, 탄화 목재입자, 입상암면(GR), 그리고 피트모스를 혼합한 4종의 배지를 만들어 플러그 트레이에 충진하여 종자를 200구 플러그 트레이에 4반복으로 파종한 후 fog 번식상에서 3일간 발아시켰다. 발아 후 양지붕형 유리온실에 난괴법으로 배치하여 저면관수로 양액을 공급하여 육묘하였다. 상업적으로 이용되는 공정육묘 배지(토실이)를 대조구로 이용하였다. 플러그묘의 생육은 파종 후 31일째 측정하였다. 모든 처리에서 유사한 생육결과를 나타냈지만 특히 CCW(67%)+peatmoss(33%) 혼합배지에서 초장, 뿌리등급, 생체중, 그리고 배지의 기상과 가비중이 다른 처리에서보다 유의성 있게 좋았다. 모든 배지의 pH는 6.3∼7.4의 범위로 측정되었다. CGF와 입상암면의 혼합비율이 증가할수록 배지의 pH는 증가하였다. 재배 후 배지의 EC는 대조구에서 가장 높았으나 독성증상은 관찰되지 않았다. 엽수와 엽록소 함량은 처리간 유의성이 없었다.

본 연구는 돼지 배아의 유리화 동결 시 CB 처리군과 CB 비처리군의 영향을 비교하였다. CB의 농도별, 처리 시간별로 처리한 뒤 유리화 동결 융해 후 정상적인 형태 회수율과 생존률을 각각 조사하였다. 1. CB를 전 처리한 군과 비처리군의 정상적인 형태율이 각각 84.2%, 81.9%로 유의한 차이가 없었으나, 융해 후 72시간 째 생존률의 결과에서는 각각 60.5%, 32.8%로 유의성(p<0.1)이 검증되었다. 2. CB 농도별 생존률에 미치는 영

This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.

본 실험은 백두산 자생 참돌꽃의 발아조건과 종자, 유식물 및 뿌리유래 캘러스의 유리 아미노산 조성과 함량을 조사하여 다음과 같은 결과를 얻었다. 뿌리로부터 캘러스 유도는 2,4-D 및 NAA가 첨가된 MS배지에서 유도되었다. 캘러스 형성율은 2,4-D 2.0 mg L-1에 kinetin 1.0 mg L-1 혼용 처리구가 NAA 2.0 mg L-1에 kinetin 1.0 mg L-1 혼용 처리구보다 캘러스 형성율이 높았

Human embryonic stem (hES) cell lines have been derived from human blastocysts and are expected to have far-reaching applications in regenerative medicine. The objective of this study is to improve freezing method with less cryo-injuries and best survival rates in hES cells by comparing various vitrification conditions. For the vitrifications, ES cells are exposed to the 4 different cryoprotectants, ethylene glycol (EG), 1,2-propanediol (PROH), EG with dime-thylsulfoxide (DMSO) and EG with PROH. We compared to types of vehicles, such as open pulled straw (OPS) or electron microscopic cooper grids (EM grids). Thawed hES cells were dipped into sequentially holding media with 0.2 M sucrose for 1 min, 0.1 M sucrose for 5 min and holding media for 5 min twice and plated onto a fresh feeder layer. Survival rates of vitrified hES cells were assessed by counting of undifferentiated colonies. It shows high survival rates of hES cells frozen with EG and DMSO (60.8％), or EG and PROH(65.8％) on EM grids better than those of OPS, compared to those frozen with EG alone (2.4％) or PROH alone (0％) alone. The hES cells vitrified with EM grid showed relatively constant colony forming efficiency and survival rates, compared to those of unverified hES cells. The vitrified hES cells retained the normal morphology, alkaline phosphates activity, and the expression of SSEA-3 and 4. Through RT-PCR analysis showed Oct-4 gene expression was down-regulated and embryonic germ layer markers were up-regulated in the vitrified hES cells during spontaneous differentiation. These results show that vitrification method by using EM grid supplemented with EG and PROH in hES cells may be most efficient at present to minimize cyto-toxicity and cellular damage derived by ice crystal formation and furthermore may be employed for clinical application.

초 록 지금까지 밝혀지지 않은 애기유리나방의 발생소장을 성페로몬트랩을 이용하여 1998년, 2001년, 2002년 3년 동안 김해와 진주의 단감원에서 조사하였다. 김해지역에서는 연 2회의 뚜렷한 발생성기를 보였다. 제1세대는 5월 중순부터 7월 상순까지, 제2세대는 7월 하순부터 9월 하순까지 발생하였는데, 발생최성기는 각각 6월 상순과 8월 상ㆍ중순이었다.