This study was performed to investigate the antidiabetic study effect in ethanol extract of Ailanthicortex(A.C) radici in Streptozotocin(STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45㎎/㎏ dissolved in citrate buffer. The ethanol extract of A.C radici was orally administrated once a day for 7 days at a dose of 500㎎/㎏. The content of serum glucose, triglyceride(T.G), total cholesterol were significantly decreased in treated group compared to the those of STZ-control group. The content of hepatic glycogen and activity of glucokinase(GK) were significantly increased in treated group compared to the those of STZ-control group. but activity of glucose-6-phosphate dehydrogenase(G-6-PDH) was significantly increased. These results indicated that ethanol extract of A.C would have antidiabetic effect in STZ-induced diabetic rats.

혈액 속의 글루코우즈의 농도를 측정하기 위해 포스파젠 고분자로 만들어진 진단막을 제조하였다. 혈액 속에 존재할 가능성이 있는 장애성분들이 글루코우즈의 농도측정에 미치는 영향을 조사하였다. 그러한 물질들 중, ascorbic acid (AA)는 기준 플라즈마 용액보다 3~9% 정도 높은 K/S 결과치를 나타내었고, tolbutamide (TA)는 기준용액보다 11~13% 정 도 낮은 K/S 결과치를 나타내었고, triglycerides와 bovine serum albumin (BSA)은 기준용액 보다 20~25% 정도 낮은 K/S 결과치를 나타내었다. 그러나 위의 물질들을 제외하고는 대부분의 장애성분들이 포스파젠 고분자 진단막을 이용한 혈당측정에 큰 영향을 미치지 않는다는 것을 보여 주었다.

To investigate the effects of coated liposome from Discorea rhizoma extract (DRE) in streptozotocin (STZ)-induced, we evaluated changes in body weight, fasting blood glucose, blood insulin and blood lipid concentrations in mice. Mice were divided into four groups: (DC), diabetic DRE at 10 ㎎/㎏ (DRE-1), diabetic DRE at 50 ㎎/㎏ (DRE-2), and diabetic DRE at 250 ㎎/㎏ (DRE-3). Mice had free access to water and diet (10 weeks). The DC group showed higher blood cholesterol than the DRE-1, DRE-2, DRE-3 groups. In glucose tolerance test, the DRE-1, DRE-2, and DRE-3 groups increased after 30 minutes in decremental glycemic response area under the curve. Fasting blood glucose levels in the DRE groups significantly decreased through 4 weeks. Plasma total cholesterol, triglyceride and LDL-cholesterol concentrations were also lower in the DRE groups. On the other hand, the DRE-1, DRE-2 and DRE-3 groups showed higher HDL-cholesterol and insulin levels than the DC group. Moreover, blood glucose and lipid levels significantly decreased in streptozotocin (STZ)-induced diabetic mice treated with DRE. These results indicate that DRE may reduce elevated blood glucose levels and serum lipid concentrations in hypoglycemic and diabetic mice, suggesting its usefulness as a functional food.

당뇨병 환자의 혈당치 측정을 위하여 포스파젠 진단막을 제조하였다. 활성화된 포스파젠 진단막을 이용해 혈액 속의 글루코우즈의 농도를 변화시켜가며 여러 온도와 습도에서 680 nm에서의 최종흡광도를 측정하였다. 측정 온도와 습도는 시간에 따른 흡광도 변화량(K/S)의 최종 결과치에 크게 영향을 주지 않았다. 여러 가지 온도에서 활성화된 포스파젠 진단막을 3일, 1주, 3주, 5주간 보관한 후 보관온도가 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 그리고 상대습도 80%에서도 포스파젠 진단막의 안정성이 확인되었다.

본 실험은 문헌상으로 혈당강하효과가 알려져 있는 인동초를 에탄올로 추출하여 그 추출물을 streptozotocin(STZ)으로 당뇨를 유발시킨 흰쥐에게 투여 후 혈당강하작용을 확인하였다. 또한, 그 유효 성분의 추적을 위하여 여러 용매(hexane, chloroform, ethylacetate, butanol, water)로 계통분획하여 그 추출물의 항 당뇨효능을 분석한 결과 hexane, water층의 분획물이 당뇨유발 흰쥐에서 glycogen 함량증가와 glucose-6-phosphatase(G-6-Pase), glucose-6-phosphate dedehydrogenase (G-6-PDH)등의 당대사효소 활성에 효과가 있었다.

After a mixed carbohydrate diet, inhibition of α-amylase and α-glucosidase involved in the digestion and absorption of carbohydrates can significantly decrease the postprandial increase of blood glucose level. In the course of screening these useful enzyme inhibitors, we selected five kinds of bean, using an in-vitro enzyme inhibition assay method. To evaluate the effect of germination process on the functionality of the bean, we investigated the inhibitory activities of the water extracts of non-germinated bean and germinated bean against α-amylase and α-glucosidase, relevant to postprandial hyperglycemia. We also investigated the oxygen radical absorbance capacity(ORAC), total phenolics content, and postprandial blood glucose lowering effect in rats(Sprague-Dawley rat model). Most germinated beans showed significantly higher α-glucosidase inhibitory activity, compared with non-germinated beans. Among germinated beans, Glycine max had the highest α-glucosidase inhibitory activity(53.3%). The water extract of germinated Phaseolus vulgaris L. had the highest α-amylase inhibitory activity(95.1%), followed by Glycine max(58.7%), and Glycine max L. Merr(54.1%). Furthermore, the five germinated beans also showed high antioxidant activities in ORAC assay. Results suggested that the germination process may improve and enhance the anti-hyperglycemia potential and antioxidant activity of the bean.

The inhibitory activities of a water extract of Sanghwang mushroom(Phellinus linteusau)(SWE) against α-glucosidases were evaluated in this study. Inhibiting these enzymes involved in the absorption of disaccharides significantly decreases the postprandial increase in blood glucose level after a mixed carbohydrate diet. Oxygen radical absorbance capacity and 1, 1-diphenyl-2-picryl hydrazyl scavenging activities of the SWE were evaluated to investigate the antioxidant activity of the SWE associated with complications of long-term diabetes. Furthermore, the postprandial blood glucose lowering effect of SWE was compared to a known type 2 diabetes drug(Acarbose®) in a Sprague-Dawley rat model. SWE significantly reduced the blood glucose increase after sucrose loading. These results suggest that SWE, which has high α-glucosidase inhibitory activity and high antioxidant activities, has the potential to contribute to a useful dietary strategy for controlling postprandial hyperglycemia.

당뇨병 환자의 혈당치 측정을 위하여 메톡시에톡시기와 트라이플루오르에톡시기가 함께 치환된 포스파젠 고분자를 기초로 한 새로운 형태의 진단막을 제조하였다. 플라즈마와 혈액속의 글루코우즈의 농도를 변화시켜가며 활성화 된 폴리포스파젠 진단막을 가지고 680 nm에서의 최종흡광도를 측정하였다. 시간에 따른 흡광도 변화량(K/S)의 최종 결과치가 글루코우즈의 농도가 증가함에 따라 직선적으로 증가하였다. 친수성기와 소수성기의 치환율이 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 친수성기의 치환율이 증가함에 따라 글루코우즈의 농도와 K/S와의 기울기 값(Dose-Response Slope : DRS)이 점차 증가하였다. 그러나 친수성기의 치환율이 30% 이상일 경우에는 DRS가 급격히 증가하여 정확한 글루코우즈의 농도 측정이 어려웠다.

This study was conducted to investigate the effect of oral administering kefir on blood glucose levels in diabetic mice. Kefir(10 ㎖/㎏body weight) was orally administered to diabetic mice twice daily for 45 days. Diabetic mice fed saline or 10% skim milk were used as a control. No significant difference in feed intake and weight gain were observed among the treatments. Compared to the saline-fed or skim milk-fed group, blood glucose in the kefir-fed group decreased significantly at 45 days(p<0.05). At 45 days, serum triglyceride and non-esterified fatty acid concentrations in diabetic mice fed kefir decreased significantly compared to those of the saline and skim milk groups(p<0.05). Both high density lipoprotein and low density lipoprotein concentrations were not significantly different among the three treatment groups. In conclusion, the symptoms of diabetic mice improved when kefir was fed for >45 days.

The present study was performed to determine beneficial effects of donkey meat extract supplementation (15 and 30 mg/kg B.W.) on streptozotocin (STZ) induced diabetic rat. Rump muscle of donkey was extracted by boiling in water for 3 hrs and supplemented to STZ induced diabetic rats for 6 weeks. Feed and water intake of STZ induced diabetic rats were higher than those of normal rats, while no significant difference was found in DME treated rats as compared to the rats in control group. STZ-induced diabetic rats did not improved their body weight as compared to that of rats in the control group. However, blood glucose level of 30 mg DME supplemented rats was found to be significantly reduced after 4 weeks of supplementation (p<0.05) when compared to the control animals. Administration of DME did not show any improving effect on serum lipids (HDL-cholesterol, total cholesterol, and triglyceride). No significant changes were noticed in terms of weight of all the visceral organs of animals fed on DME except liver. It is therefore, concluded that supplementation of DME with dose of 30 mg/B.W. resulted in decreased blood glucose levels, however, blood lipid profile of diabetic rats, having more than 400 mg/dl glucose level, did not change.

The objective of this study was to assess improvements caused by silk sericin extract in blood glucose and lipid concentrations in the sera of streptozotocin(STZ, 55㎎/㎏B.W., I.P. injection)-induced diabetic rats fed on experimental diets for 5 weeks. Serum concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, triglyceride(TG) and phospholipids(PL) were remarkably higher in the diabetic group(group BSW) and STZ(I.P.)-plus-silk sericin extract group(group ESS) than in the control group(group CG, basal diet-plus-water). However, the serum concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, TG and PL were lower in the ESS group than in the BSW group, whereas the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration in the ESS group were higher than in the BSW group. The activities of alkaline phosphatase(ALP) and aminotransferase(AST, ALT) in serum were lower in the ESS group than in the diabetic BSW group. The results shown above suggested that silk sericin extract effectively improves blood glucose and lipid concentrations in the sera of STZ-induced diabetic rats.

당뇨병 환자의 혈당치 측정을 위하여 폴리우레탄으로 만들어진 진단막을 제조하였다. 혈액 속의 글루코우즈의 농도를 변화시켜가며 폴리우레탄 진단막을 가지고 680 nm에서의 최종흡광도를 측정하였다. 시간에 따른 흡광도 변화량(K/S)의 최종 결과치가 글루코우즈의 농도가 증가함에 따라 직선적으로 증가하였다. 헤마토크릿이 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 낮은 헤마토크릿에서는 글루코우즈의 농도와 K/S와의 기울기 값(Dose-Response Slope : DRS)이 플라즈마와 비교해 큰 차이를 보이지 않았다. 그러나 높은 헤마토크릿(40% 이상)에서는 상당히 감소함을 알 수 있었다.

This study was conducted to examine the hypoglycemic effects of complexcity extract(DB 55) from five oriental medicinal plants on the intake of food, body weight, blood glucose level, insulin content, serum GOP, GPT, BUN and hematocrit value in streptozotocin(STZ)-induced diabetic rats. Genera1 nutritional composition was also assessed. Thirty-five male Sprague-Dawley rats were assigned to five groups: normal control group(NC), STZ-diabetic control group (STZ-control), STZ-diabetic DB-1 group(DB 55-1), STZ-diabetic DB-2 group(DB 55-2) and STZ-diabetic DB-3 group(DB 55-3). The animals were fed an experimental diet for 12 weeks. Body weight gain of the STZ-diabetic group was significantly lower but diet intake was significantly higher than the NC group. Blood glucose levels of the DB-1, DB-2 and DB-3 rats were significantly lower than the STZ-control animals. Insulin level of STZ-control rats was lower than the NC serum GOT, GPT and BUN levels were significantly higher in the NC group, but hematocrit value was not significant as compare to the STZ-diabetic group. Blood glucose level was lower observed for the DB 55-1, DB 55-2 and DB 55-3 group. The results will be useful in oriental diet therapy and in the developing functional food resources.

Carnosine is a dipeptide (β-alanyl-L-histidine) found in mammalian brain, eye, olfactory bulb and skeletal muscle at high concentrations. Its biological functions include antioxidant and anti-glycation activities. The objectives of this study were to investigate anti-diabetic effects of carnosine as determined by blood glucose levels in glucose tolerance test (GTT) and insulin tolerance test (ITT), insulin level and serum biochemical and lipid levels in male C57BL/6J db/db mice. There were five experimental groups including normal (C57BL/6J), control (vehicle), and three groups of carnosine at doses of 6, 30, and 150 mg/kg b.w. Carnosine was orally administered to the diabetic mice everyday for 8 weeks. There was no significant difference in body weight changes in carnosine-treated groups compared to the control. The treatments of carnosine significantly decreased the blood glucose level in the diabetic mice compared with the control (p < 0.05) after 5 weeks. The treatments of carnosine also significantly decreased the blood glucose levels in GTT and ITT and glycosylated hemoglobin (HbA1c), compared with the control (p < 0.05). Carnosine at the dose of 6 mg/kg significantly decreased the serum insulin level compared to the control (p < 0.05). Carnosine significantly increased total proteins but significantly decreased lactate dehydrogenase and blood urea nitrogen compared with the control (p < 0.05). Carnosine also significantly decreased glucose, LDL, and triglyceride in the serum of diabetic mice compared to the control (p < 0.05). These results suggest that carnosine has a hypoglycermic effect resulting from reduction of glucose and lipid levels and that high carnosine-containing diets or drugs may give a benefit for controlling diabetes mellitus in humans.