1997년 11월부터 1998년 6월까지 총 4회에 걸쳐 군산인근 해역을 대상으로 Vibrio 속의 분포와 특성을 조사하였고, 분리동정된 Vibrio 속 3종(V. anguillarum, V. vulnificus, V, metschnikovii)을 대상으로 해수에서 수온 변화에 따른 생존을 관찰하였다. 조사 기간 중 총 해양 종속영양 세균의 분포는 평판도말법으로는 1.2±0.6×103~2.0±1.5×104

아세트산, lauric acid, monolaurine 그리고 이의 조합에 의한 침지법을 이용하여 15% 저장 동안 광어의 V. cholerae의 세균수 변화에 대한 영향을 조사하였다. 대조구는 증류수에 3분 침지후 실험에 사용하였다. 모든 처리구에서 처리직후 V. cholerae의 세균수는 유의적 (P<0.05)으로 감소하였다. 저장 2일 이후 lauric acid 및 monolaurine의 처리구는 V. cholerae의 수를 감소하는데 있어서 초산의 처리구와 유의적 차이를 나타내었다. 1.0%의 초산에 3분 침지후 0.5% lauric acid로 3분 침지한 처리구는 0.5%의 초산에 3분 침지후 0.5% aluric acid로 3분 침지한 처리구 보다 V. cholerae의 세균수를 유의적 (P<0.05)으로 감소하였다. 0.5%∼1.0%의 초산침지후 0.05% monolaurine으로 3분 침지한 처리구는 저장 3일 이후 V. cholerae에 대한 세균수 감소효과가 나타나지 않았다.

V. cholerae non-O1 49균주와 V. cholerae O1, V. mimicus, V. vulnificus와 V. parahaemolyticus의 균체지방산(fatty acid methyl ester; FAME)을 gas liquid chromatogrphy로 분석하였다. 이들 분석자료를 통계학적으로 처리하여 Vibrio 종과 V. cholerae의 혈청형별 유연성을 비교 검토하였다. 검출된 지방산은 모두 41종이었고 분포량이 많은 것은 16:0, 16:1 cis 9, 18:1 trans 9/16/cis 11과 15:0 iso 2 OH/16:1 cis 9 였다. 검출된 지방산 중에서도 35종은 V. cholerae를 동정하는데 주요한 인자로 작용되었다. 지방산분포를 UPGMA(비가중수리분석)으로 dendrogram을 작성한 결과 V. cholerae non-O1은 V cholerae O1보다 V mimieus가 더 높은 유사도를 나타내었다. 특히 hydoxy acid는 V. chozerae의 아종단위를 동정하는데 중요하였다. V. choEeore non-O1중에서 O2, O5, O8, O10, O14, O27, O37, O39, O45와 O69의 총 10 종류 혈청형을 대상으로 지방산 조성에 의 한 유사성을 검토한 결과 유사도가 92% 이상 수준에서 7개의 아종를 형성하여 혈청형과 지방산 조성간에는 유의할 만한 상관관계가 있었다. 따라서 V. cholerne non-O1의 동정 및 역학적인 조사시 지방산 분석은 유용하게 활용될 수 있음을 알 수 있었다.

유기산 처리법을 이용하여 15℃ 저장 동안 생굴의 V. vulnificus strain 29307의 세균수 변화에 대한 영향을 조사하였다. 각각 3분 동안 0.5% 초산, 0.5% 유산, 또는 0.5% 구연산으로 침지한 생굴은 저장 4일 후부터 V. vulnificus가 검출되지 않았다. 2% 초산 함유한 3% 알지닉산 처리구는 저장 2일 후부터 V. vulnificus가 검출되지 않았으며, 수돗물로 처리한 대조구는 저장 4일동안 V. vulnificus가 분리되었다. 본 연구의 결과를 토대로 알지닉산과 초산의 조합은 각 유기산 처리구보다 V. vulnificus에 대한 항균력을 증진하였다.

Vibrio mimicus is a closely related species with V. cholerae, and has been reported to be associated with gastrointestinal infections. Although extraintestinal infections of these vibrios have also been reported in Japan and Southeast Asia. But little research papers on V. mimicus was reported in Korea. Therefore, we tried to isolate V. mimicus from the environmental sea water from April to July in Pusan, Korea. Among the isolated strains, we selected the strongest hemolytic strain and then named V. mimicus SM-9. In this paper, we checked the antibiotic susceptibility and psychrotrophic characteristics of the isolated strain. Hemolytic activity of the hemolysin produced by the isolated strain was also measured. V. mimicus was not detected from the sea water samples in April and May, but its detection rate was relatively high in June and July in Pusan, Korea. The bacteriological characteristics of V. mimicus SM-9 were Gram-negative rods, motile, oxidase positive, Voges-Proskauer negative and sucrose negative. In 23 kinds of antibiotics susceptibility test, V. mimicus SM-9 showed susceptibility to the most of antibiotics submitted while it was resistive against lincomycin, oxacillin, rifampin and vancomycin. Hemolytic activity of the hemolysin produced by V. mimicus SM-9 was highest in stationary phase of the growth curve in BHI broth at 37℃ and its activity was reached 18 HU per ml of culture supernatant. For checking the psychrotrophic property of V. mimicus SM-9, the decreasing rate of the strain in phosphate buffer solution and yellowtail flesh homogenate was examined during the storage at 4, 0, -4 and -20℃. The decreasing rates of the selected strain stored in phosphate buffer solution were greater than those in fish homogenate. Decreasing rates of V. mimicus SM-9 stored in phosphate buffer solution were not significantly different by the storage temperatures. The viable cell counts of the strain were decreased as 5 log cycles after 120 hours at all the tested temperatures. While decreasing numbers of the strain in fish homogenates were 2-4 log cycles after 120 hours. The decreasing pattern of the strain numbers were very slow after 200 hours at all the stored temperatures.

Vibrio vulnificus, a normal inhabitant in estuaries, is of great concern because it is a potent human pathogen causing septicemia, wound infection and gastrointestinal disease in susceptible hosts. To elucidate the distribution and environmental factors of V. vulnificus in Kum river, sampling was undertaken in five station from March, 26, 1993 to February, 22, 1994. Samples of 54 and 49 were collected from seawater and bottom deposit. The total detection rate of V. vulnificus was 11.7%. The detection rates of V. vulniftcus in the seawater and the bottom deposit were 9.3% and 14.3% respectively. V. vulnificus was mainly detected in estuary water when temperature was above 23℃ and salinity was below 15‰. We suppose that water temperature, salinity, pH and COD affect growth of V. vulnificus.

Vibrio vulnificus에서 lipopolysaccharide(LPS)를 추출하여 지방산 조성을 분석한 후 이 결과를 Escherichia coli LPS와 Salmonella typhimurium LPS의 것들과 비교하였다. Vibrio vulnificus LPS의 주 지방산은 myristic acid(C14:0, 41.37%)이었고 Escherichia coli LPS는 lauric acid(C12:0, 37.03%), Salmonella typhimurium LPS는 capric acid(C10:0, 48.60%)로 서로 달랐으나 이 세가지 지방산이 각 LPS의 주성분이었다(70%이상).

The objective of this research was to evaluate the toxicity of the industial xenobiotic Aroclor 1248 (A) and natural origin substances~elemental sulfur (S80) and oleic acid (OA) and their binary mixtures to V. fischeri bioluminescence during the prolonged exposure time (up to 60 min). The bioluminescence quenching test was used to determine the toxic effects. Full factorial experiment design and multiple regression analysis and the comparison of binary mixture effect with the sum of effects of individual chemicals were used for the evaluation of combined effects of toxicants. The analysis of general trend of mixture toxicity to bioluminescence showed that mixture toxic effects were reversible up to 60 min. Data analysis revealed different joint effects, which were depended on mixture composition. S80 enhanced toxic effect of A and acted additively with synergistic interaction. Hydrophobic OA in mixture with A acted antagonistically and in mixture with sulfur caused an additive effect with antagonistic component of interaction. It was concluded that low concentrations of natural toxic substances present in environmental samples as mixtures of chemicals can define the toxicodynamic character of industrial xenobiotics.

The role of iron as a possible pathogenic factor in the infection of V. vulnificus was examined in this paper. The effects of iron and CCl_4 on the growth of V. vulnificus in human and rabbit sera were also done. Injection of iron to mice resulted in a lowering of the 50% lethal dose and in a reduction in the time of death postinfection. Serum iron levels were also elevated by damaged livers with infections of CCl_4. The inoculum size required to kill these mice was directly correlated with serum iron levels. Iron appeared to be the limiting factor in the ability of this organism to survive or grow in mammalian sera. These results, both in vitro and in vivo, provided strong evidence that iron may play a major role in the pathogenesis of V. vulnificus.

This project studies marine Vibrio vulnificus in oysters in the marine environment and attempts to correlate this bacteria`s presence within various environmental parameters; we design this study to determine how different storage temperatures affect the survival of V. vulnificus in oysters and whether V. vulnificus is able to persist in oysters after exposure to UV light-disinfected seawater. Experimental depuration systems consist of aquaria containing temperature-controlled seawater treated with UV light and 0.2 ㎛ pore size filtration. Results showed that depuration at temperatures higher than 25℃ caused V. vulnificus counts to increase in oysters. Throughout the process, depuration water contained high concentrations of V. vulnificus, indicating that the disinfection properties of UV radiation and 0.2 ㎛ pore size filtration were less than the release of V. vulnificus into seawater. In contrast, when depuration seawater was maintained at 10℃, the numbers of V. vulnifcus were very little and multiplication in oysters was inhibited.

This study was conducted to investigate the effects of environmental factors such as temperature, salinity, pH, and UV light on the survival of life-threatening Vibrio vulnificus. In the temperature range of 15 to 25℃, the numbers of V. vulnificus increased during the 6-day incubation, but outside this range, the survival of V. vulnificus was poor. Incubation between 1 and 10℃ showed that V. vulnificus survived poorly below 10℃. At salinities between 5 and 25ppt, the numbers of V. vulnificus increased or remained unchanged for 6-day. At salinities above this range, the numbers of V. vulnificus decreased. The optimal pH range was 6.5 to 8.0 and outside this range, the survival ratio of V. vulnificus was small. At 15 and 25℃, UV radiation was bactericidal for cultures of V. vulnificus. The counts of V. vulnificus were reduced approximately 10,000-fold after 2h of UV light treatment at both temperatures. Above results showed that environmental factors were effective on the survival of V. vulnificus in the environment.