본 연구는 사료 내 총NDF 수준과 조사료에서 기인된 NDF 수준(fNDF)이 착유우에서의 영양소 섭취량과 유생산 성에 미치는 영향을 평가하기 위하여 실시하였다. 분만우 36두를 대상으로 6개의 처리구를 나누어 2×3 요인실험으로 설계를 하였고, 각 처리구별 사료는 건물기준 NDF 38%에 1) fNDF 18%, 2) fNDF 15%, 3) fNDF 12% 와 건물기준 NDF 34%에 4) fNDF 18%, 5) fNDF 15%, 6) fNDF 12%로 구분하여 분만 후 3주부터 20주까지 급여하였으며 결과의 처리는 분만 4주부터 영양소 섭취량 및 산유성적을 분석하 였다. 사료 내 총 NDF 함량과 fNDF 함량이 감소함에 따라 건물섭취량, NEl 섭취량 그리고 비구조탄수화물 섭취량이 유의적으로(p<0.01) 증가하였고, 통계적인 유의성은 없었으 나 유생산량 또한 다소 증가하는 경향이었으며 유지방율은 감소하였다(p<0.01). 건물섭취량의 증가에 따른 NEl 섭취 량의 증가가 유발되었고, NDF 섭취량은 감소하였음에도 불구하고 NDF 함량이 낮은 처리구와 높은 처리구간의 비 교에서 유지방율에 대한 유의적인 차이를 나타내지는 않았 다. 총 NDF와 조사료유래 NDF의 사료 내 함량변화는 유 단백질율과 무지유고형분 함량에는 영향을 미치지 않았지 만, 유단백질 함량과 무지유고형분 생산량에 있어서는 유 량 증가에 의한 영향으로 증가하는 경향을 나타내었다. 조 사료유래 NDF 섭취량과 유지방율 간의 상관관계는 저수준 NDF 함량에서 고수준 NDF 함량보다 높게 나타났으며 유 량과 영양소 섭취량간의 상관관계 또한 저수준의 NDF 처 리구에서 고수준 처리구보다 높게 나타났다.

The objective of this study was to analyze the accuracy of estrus detection of heat detector and analysis of estrus behavior (mounting and mounted), and the evaluation of conditions required for improving reproductive efficiency in Holstein dairy cows fitted with a estrous detector. The heat detection system consists of estrous detector based on wireless sensor and an electric bulletin board displayed estrus behavior data. When cow mounting other cows, the accuracy of estrus behavior displayed an electric bulletin board were 87.5% (mounting other cows only), 100% (mounting other cows but not standing), 80.0% (mounting other cows with standing for 1∼4 seconds), 90.0% (mounting other cows but not standing for 1∼4 seconds), 80% (mounting other cows with standing for more than 5 seconds) and 90.0% (mounting other cows but not standing for more than 5 seconds). When cow mounted other cows, the accuracy of estrus behavior displayed an electric bulletin board were 100% (mounted other cows but not standing), 100% (mounted other cows with standing for 1∼4 seconds), 100% (mounted other cows but not standing for 1∼4 seconds) and 100% (mounted other cows with standing for more than 5 seconds). Circadian distribution of first observed in estrus were 59.1% (am 8∼pm 6) and 40.9% (pm 6∼am 8). Distribution for the number of estrus behavior were 40.9% (less than 3 times), 36.4% (4∼6 times) and 22.7% (more than 4 times). The conception rates relative to interval from first estrus behavior to insemination for estrus periods were 23.1% (less than 11 hours) and 55.6% (12∼20 hours).

The aim of this study was to evaluate the changes of protein patterns in granulosa cells and corpus luteum in ovaries during the estrus cycle in cows. The estrus cycle was devided into five steps of follicular, ovulatory, early-luteal, mid-luteal and late-luteal phases. In results, 61 spots of total 85 spots were repeated on follicular phase and 51 spots of total 114 spots were repeated on ovulatory phase. The 40 spots of total 129 spots were repeated on early-luteal phase and 49 spots of total 104 spots were repeated on mid-luteal phase. Also 41 spots of total 60 spots were repeated on late-luteal phase. On the other hands, the 16 spots were indicated difference in follicular phase and ovulation phase had a difference 10 spots. It was showed difference No. 103 spot in ovulation phase, No. 135 spot in early-luteal phase and No. 175 and 176 spots in mid-luteal phase. Also, the 11 spots were expressed specifically in mid-luteal phase and No. 178 and 179 spots were difference of expression in late-luteal phase. We confirmed that there were 7 spots for ovulation, 4 spots for luteinization and 2 spots for luteolysis. Spot No. 89～93 in ovulation phase were transferrin, and spot No.94～98 were HSP60. Spot No. 103 was Dusty PK, spot No. 135 was OGDC- E2, and spot No. 175 and 176 were Rab GDI beta from luteinization. Spot No. 178 and 179 in luteolysis were vimentin. This results suggest that will be help to basic data about infertility.

This study aimed to improve the reproductive efficiency of dairy herds by comparison and analyzing estrous appearance rate, conception and non-conception rate according to the stage of lactation using the lactation and reproductive records of average (less than 10,000 liters milk in 305 days) and high yielding (more than 10,000 liters milk in 305 days) Holstein cows (n=102). Milk production and reproduction data were collected between January 2010 and December 2012 from Holstein cows kept in the commercial dairy farms. Average (n=32) and high yielding (n=24) Holstein cows used to analyze the relationship between milk yield and reproductive performance. Our results showed that estrous appearance rate according to the stage of lactation was 25.0% (30∼59d), 40.6% (60∼ 89d), 25% (90∼110d) and 9.4% (>111d) in average yielding cows and 16.7% (30∼59d), 20.8% (60∼89d), 12.5% (90 ∼110d) and 50.0% (>111d) in high yielding cows, respectively. Conception rate according to the stage of lactation was 87.5% (30∼59d), 61.5% (60∼ 89d), 75.0% (90∼110d) and 66.7% (>111d) in average yielding cows and 25.0% (30∼59d), 0% (60∼89d), 33.3% (90∼ 110d) and 50.0% (>111d) in high yielding cows, respectively. Days between parturition and conception was 23.7% (<149d), 0% (150∼209d) and 0% (>210 d) in average yielding cows and 69.0% (<149 d), 77.8% (150∼209d) and 38.9% (>210d) in high yielding cows, respectively. Conception rate from 110 days postpartum in high yielding cows was 41.7% (110∼150d), 50.0% (151∼180d) and 50.0% (>181d). Body condition score (BCS) in 120 days postpartum was 2.64±0.1 in average yielding cows and 2.28±0.1 in high yielding cows, respectively.

An experiment was conducted to study effects of abomasal infusions of casein, glucose or starch on milk production and blood metabolites in dairy cows. Four lactating cows (559±41.9kg) fitted with 100 mm ruminal cannulas were used in a 4 × 4 Latin square experiment. The cows were given access ad libitum to grass silage and received 6.2 kg dry matter (DM) of a mixture of rapeseed meal (5.3kg/d DM) and barley grain (0.9kg/d DM) as a basal diet (CON), the basal diet plus intra-abomasal infusions of 270 g sodium caseinate (CAS), 300 g of glucose (GLU) or 243 g of starch (STA) starting at 09:00 h each day. Abomasal infusion of different nutrients did not affect (p>0.05) grass silage intake and rumen fermentation parameters (rumen pH, ammonia N and volatile fatty acids). As unexpected, milk yield and composition data did not differ between CON and the treated groups (p>0.05). Most of plasma amino acids were not significantly (p>0.05) affected by the abomasal infusions of CAS, GLU or STA except for histidine, tryptophan and glycine. Although histidine, known as the first limiting amino acid in dairy cows fed grass silage based diets, was significantly (p=0.003) increased by CAS, we failed to result in a clear increase in milk yield and protein yield. This discrepancy between plasma AA profiles and milk production could be presumably explained by relatively high concentration of total amino acids in the present study (2,039μmol/l).

The objective of this study was to investigate the effects of abnormal ovarian cycles after superovulation treatment of Holstein Donor Cows. CIDRs were inserted into the vaginas of twenty two head of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with PGF2α and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. The cows were considered to have resumed ovarian cyclicity on the day of ovulation if followed by regular ovarian cycles. Seventy two point seven percentage of the cows(16/22) had normal resumption of ovarian cyclicity(resumption within 40 days after superovulation), and 27.3%(6/22) had delayed resumption(resumption did not occur until＞40 days after superovulation). Delayed resumption Type Ⅱ(first ovulation did not occur until ≥40 days after superovulation, i.e. delayed first ovulation 13.6%) were the most common types of delayed resumptions. The mean numbers of total ova from < 10 and 10≤ of corpora lutea(CL) was 7.8±1.8 and 12.7±2.7, respectively. The number of transferable embryos differed between < 10 and 10≤ CL was 5.4±1.3 and 8.1±3.4, respectively. Four point five percentage of the cows(1/22) did not resumption their ovarian cyclicity until 60 days after superovulation treatment. Diverse researches on the superovulation treatment method that is suitable for high-producing Holstein donor cows would contribute to preventing ovarian cyclicity disorder, as well as to the early multiplication of cows with superior genes by increasing the utilization value of donor cows.

This study was carried out to investigate the influence of artificial insemination (AI) failure in 1,619 Korean native cows at Gangwon East area, Korea. The average AI failure rate was 37.02% in the cows and the highest rate was 40.85% in Yangyang-city. Based on the parity in the cows, the AI failure rate was 49.14% and 29.91% in the first and fifth parity cows, respectively. Whereas cows until fifth parity were decreased in AI failure, cows with sixth or more parity showed an upturning AI failure trends with the increasing of parity number. AI failure rate incidence according to the rump fat thickness measured by ultrasound was 28.9% and 33.4% at 5 mm to 10 mm and over than 15 mm, respectively. There was a positive correlation (0.2186) between AI failure rate of mother and that of their offspring cows. That is, offspring of dams with high AI failure rate showed also higher AI failure than those of dams having lower AI failure rate. In conclusion, these results indicate that the AI failure rate was closely related to the rump fat thickness, parity number, and conception rate of mother cows. In addition, these results might strengthen the basis to improve the reproductive performance in Korean native cows.

본 실험은 조사료와 농후사료비율(조:농비)에 따라 low forage (LF)구 및 high forage (HF)구로 나누어 Holstein 착유소(초산) 10두를 각 5두씩 배치하여 실시하였다. LF구는조:농비가 37:63로 BIRG 사일리지와 혼합건초를 포함한조사료의 급여량을 줄인 반면 농후사료를 다량 급여하였다.HF구는 조:농비가 62:38로 농후사료 급여량을 줄인 반면BIRG 사일리지와 혼합건초를 포함한 조사료를 다량 급여하였다. 평균 조사료섭취량은 LF구 7.1및 HF구 13.0 kg으로 HF구에서, 평균 농후사료 섭취량은 LF구 12.3 및 HF구7.9 kg으로 LF구에서 유의적으로 높았다(p<0.05). 총 사료섭취량은 LF구 19.4 및 HF구 20.9 kg으로 HF구에서 높은경향을 보였으나 유의적인 차이는 없었다(p>0.05). CP,TDN 및 NEL 섭취량은 두 처리구간에 유의적인 차이는없었다(p>0.05). 실제 산유량은 LF구 26 및 HF구 23.9 kg/d으로 처리 간에 유의적인 차이는 없었으나(p>0.05) LF구에서 높은 경향을 보였다. 4% FCM은 LF구 22.8 및 HF구22.3 kg/d 으로 유의적인 차이는 없었다(p>0.05). HF구에서유지방 함량은 높고 MUN 함량은 낮았다. 우유의 C14:0,C16:0 및 C16:1n7 함량은 HF구가 LF구 보다 유의적으로높았으나(p<0.05), 그 외의 우유지방산은 두 처리구간에 차이는 없었다. 이상의 결과로부터 본 연구에서와 같이 급여사료 중 BIRG 사일리지와 수입건초와 같은 조사료를 적절하게 배합하여 다량급여 하여도 착유소(초산우)에 필요한영양소 충족이 가능하여 산유량의 감소가 없는 것으로 사료된다.

This research was investigated the relationship between the number of the transferable embryos and estrus expression rate, BCS (Body Condition Score), which affect the nutritional state of the cow, in Holstein donor cows. CIDRs were inserted into the vaginas of twenty two head of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. When BCS was 2.5, the total number of collected ova was 7.3 + 1.9, which is significantly lower (p<0.05) than the numbers 15.4 + 2.8 and 15.4 + 2.1 that were obtained when BCSs were 2.75 and 3.0, respectively. Whereas the numbers of transferable embryos were 5.2 + 1.4 when BCS was 2.5, which was smaller than the numbers 6.0 + 2.1 and 8.5 + 1.8 that were obtained when BCSs were 2.75 and 3.0, respectively; however, the differences were not significant. As for estrus induction rate, the cow groups whose BCSs were 2.75 and 3.0 showed 100.0% and 95.0%, respectively. Whereas the cow group whose BCS was 2.5 showed 57.1%, and the differences were significant (p< 0.05). As for estrous expression rate, the cow groups whose BCSs were 2.5, 2.75 and 3.0 showed 100.0%, 100.0% and 85.7%, respectively; however, the differences were not significant. According to the result of this research, it is considered that the total number of collected ova and the number of transferable embryos will be affected by the nutritional state before and after in vivo embryo production and superovulation treatment, and that although the mechanism is not clear, poor stockbreeding management and nutritional level would cause the decrease of ovum recovery rate and the number of transferable embryos in high-producing cows. On the other hand, diverse researches on the superovulation treatment method that is suitable for high-producing Holstein donor cows would contribute to preventing ovarian cyclicity disorder, as well as to the early multiplication of cows with superior genes by increasing the utilization value of donor cows.

This research was investigated the relationship, in high-producing Holstein donor cows, between the number of the transferable embryos and the blood serum concentrations of Blood Urea Nitrogen (BUN), glucose and cholesterol, which affect the nutritional state of cows. CIDRs were inserted into the vaginas of twenty two heads of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. The total numbers of ova collected from 3 experimental groups whose blood BUN concentrations were <10 mg/dl, 11~18 mg/dl and mg/dl were 8.9, 12.5 and 19.0, respectively; whereas the numbers of transferable embryos were 5.8 + 1.9, 7.9 + 2.8 and 5.2 + 1.4, respectively. When glucose concentration was <60 mg/dl, the total number of collected ova was 9.9, which was smaller than when the concentration was 60~70 mg/dl or mg/dl. When glucose concentration was 60~70 mg/dl, the number of transferable embryos was 7.1 + 2.4, which was slightly larger than the numbers 6.4 + 2.1 and 6.1 + 1.7 that were obtained when the concentrations were <60 mg/dl and mg/dl, respectively ; however, the differences were not significant (p>0.05). When cholesterol concentrations were <150 mg/dl, 150~200 mg/dl and mg/dl, the total numbers of collected ova were 11.2, 11.3 and 8.6, respectively. Whereas the numbers of transferable embryos were 7.1 + 2.1, 7.3 + 1.9 and 5.6 + 1.3, respectively ; however, the differences were again not significant (p>0.05). The result of this research showed no significant difference in ovum recovery rate and the number of transferable embryos according to major metabolite concentrations in high-producing Holstein donor cows. However, it is considered that the failure of maintaining proper nutritional status would cause the fall in in vivo embryo productivity.

Bovine brucellosis causes abortion and infertility. The authors conducted this study in order to determine pathological lesions of Korean native cows and fetuses who received experimental vaccination with Brucella abortus RB51 and were challenged with Brucella abortus 2308. Gross and histopathological lesions in endometrium and placenta were observed in cows of the vaccinated group. Twenty-five percent of pregnant cattle in the vaccinated group showed endometritis and placentitis, which was three times lower, compared with the non-vaccinated group. The pathological lesions in the uterus and placenta in both groups were consistent with previous reports. Therefore, vaccination in heifers using Brucella abortus RB51 may not provide adequate protection against infection with Brucella abortus virulent strain.

This study was operated to establish induction using ultrasonography by estimating the relation of follicle size and estrus manifestation. Clinical estrus symptoms were observed 97.4% in cows and 87.5% in heifers when overall 55 cows were induced to estrus in a single dose of after verifying CL through ultrasonography, which means estrus hours among those 52 cows showing the clinical estrus symptoms were estimated 2.39 days on cows and for 2.37 days on heifers which showed no differences (p>0.05). The estrus manifestation hours according to the follicle size in cows didn't have any significance each other (p>0.05), though estrus hours was 54 hours (the shortest) with follicle size bigger than 10 mm and were made up within 69 hours. The estrus manifestation hours according to the follicle size in heifers didn't have any significance each other (p>0.05) and took around 42 hours (the shortest) with follicle size of 5mm (the smallest) and were made up within 66 hours. Follicles after injection were ovulated and assigned to many phases as follows; Group 1 (growing phase) - continuously growing into ovulation, Group 2 (growing and static phase) - delaying in growth after the growth of follicles, Group 3 (static and growing phase) - growing after growth delay, Group 4 (regressing and new growing phase) - the follicle is closed and a new follicle grows. In addition, the process of follicle development and estrus hours had no significance each other (p>0.05), though estrus manifestation hours in Group 1 and 2 was relatively short, and in Group 3 and 4 for a relatively long time. In the result of all above, the estrus manifestation hours after injection has no differences accoring to the follicle size in cows and heifers. Therefore, High pregnancy rate is obtained when practicing artificial insemination within 3 days in estrus or TAI in 72 to 80 hours after adminitrating .

This study was carried out to develop the useful inducing method of estrus for Korean native cows. Under the condition of estrus induction by administering for the cows in which corpus luteum (CL) in ovaries was detected by ultrasonography, ovarian responses and the changes of progesterone () concentration against compared with conception rate were observed in cows and heifers. In inducing estrus administering . to the cows which has corpus luteum in ovaries, ovarian reponses, the changes of progesterone concentration, and conception rate were identified and compared. The results attained from the studies were as follows. Significant decreases of CL in size over time after administration were detected in both cow and heifer groups (p<0.001), but not different between groups in the CL regression rate (p>0.05). In addition, the percentage changes relative to the plasma concentration on day 1 after treatment were decreased to below 1ng/ml. The growth rate of follicle was observed as 31% on day 1 and 42% on day 2 in cows, and 34% on day 1 and 97% on day 2 in heifers, resulting that growth of heifers are faster than that of cows (p<0.05). The conception rate after treatment were 60.5% and 64.2% in cows and heifers, respectively. It also indicated that the conception rate after estrus observation with injection was as high as 66.6% while that with timed-artificial insemination (TAI) regardless of the estrus observation was 56%, which means the pregnancy rate of artificial insemination after estrus observation was higher than that of TAI (p<0.05). In the result of all above, there were significant decreases in CL size and the plasma concentration by days but rapid growth in follicles, which has no differences in cows and heifers. The conception rate was commonly high after estrus observation and more than 50% under TAI.

A study on estrus synchronized dairy cows using progesterone intravaginal device was done to classify each cow's reproductive status from calving to synchronization and to evaluate the reproductive performance according to ovarian and uterine status, and calving season. From calving to estrus synchronization, silent heat or error of estrus detection among ovarian status and endometritis among uterine disorders were exposed in the most distribution (75.4% and 48.3%, respectively). The pregnancy rate of cows with inactive ovaries was lower than those in the follicular and luteal phase. And according to the uterine status before estrus synchronization, the pregnancy rate was similar in three groups; normal, endometritis, and pyometra (70.9, 69.1 and 100%, respectively). The interval from calving to conception was shorter (p<0.05) in cows calved during autumn than in cows calved during spring and winter.

In ruminants, Interferon-τ (IFN-τ) has the role of recognizing pregnancy signals produced by the embryo and it may have an important role during the luteolysis. Therefore, the purpose of the present study was to investigate the effect of IFN-τ on prostaglandin synthesis, cyclooxygenase-2 (COX-2) gene expression in vitro and secretion of progesterone (P4) in vivo. The epithelial and stromal cells isolated from bovine endometrium were cultured with different doses of IFN-τ (0, 0.02, 0.2 and 2 μg/ml). Human chorionic gonadotropin (hCG, 1.5 IU/ml) was used as a positive control. Prostaglandin E2 and F2α levels in the culture media were analyzed by enzyme immunoassays, and total RNA was extracted from the cells for RT-PCR. P4 concentrations in blood samples were assayed by chemiluminescent immunoassay system. In epithelial cells, COX-2 gene expression was increased in the presence of IFN-τ (p<0.05), but it was not significantly different in all groups of stromal cells except 2 μg/ml IFN-τ group (p<0.05). Although IFN-τ did not affect PGE2 and PGF2α production in epithelial cells, it decreased PGE2 and PGF2α production significantly in stromal cells (p<0.05). In vivo experiment, the P4 concentrations in blood sample was significantly increased after injection of 1 μg/ml IFN-τ. These results indicate that PG production was mediated by COX-2 expression in the stromal cells but it did not affect in the epithelial cells, and suggest that treatment of IFN-τ was to improve the implantation environment of uterine by maintenance of high P4 concentration. * This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ907008)” Rural Development Administration, Republic of Korea.

Our objective in this study is to assess the safety of docosahexaenoic acid (DHA) fortified milk of dairy cows fed feeds containing protected fish oil treated with formaldehyde by analyzing formaldehyde concentration in commercial milk and DHA fortified milk of dairy cows fed formaldehyde treated feed. There are 3 milk samples in this study: Commercial milk (CM), DHA fortified milk for Kid (DHA-K) and DHA fortified milk for Baby (DHA-B). We confirm the fresh quality of these three samples by physicochemical tests. In fat content result, three groups are significantly different at the p < 0.05 by Duncan's multiple range test, but fat content of group DHA-K is about half the level of the other two groups. Protein content of group DHA-K is 1% higher than other two groups. According to the analysis result of DHA content of DHA fortified milk, DHA content of DHA-B is two-fold higher than DHA-K. Similar pattern was seen in the intake based on age. According to HPLC analysis result of formaldehyde concentration in milk, commercial milk and DHA fortified milk are between 0.013 ppm and 0.057 ppm which is formaldehyde standard level in fresh milk settled in WHO (World Health Organization). Three groups have no significantly differences at the p < 0.05 by Duncan’s multiple range test. For this reason, it can be concluded that there is no transition of formaldehyde from dairy cows fed formaldehyde treated feeds to its produced milk. Safety about formaldehyde of DHA fortified milk of dairy cows fed formaldehyde treated feeds is considered similar to commercial milk.

본 시험은 포도부산물의 첨가가 젖소의 산유량, 유성분 및 혈액 성상에 미치는 영향을 조사하기 위하여 실시하였다. 비유중기 젖소 8두(평균 월령: 89.8, 평균 유기: 164.4, 평균 산차: 2.8산)를 공시하였으며, 포도부산물을 첨가하지 않은 대조구와 1일 1 kg을 첨가한 시험구로 두 처리구를 설정하여 3주간 급여하였다. 유지방, 유단백, 유당 및 총 고형물 함량은 처리간에 차이가 없었다. 그러나 산유량은 포도부산물 을 첨가한 처리구가 23.13 kg/d으로 대조구의 21.16 kg/d에 비해 높은 반면(p<0.05), 체세포 수는 대조구가 포도부산물 처리구에 비해 높았다(p<0.05). 혈중 glucose, alanine aminotransferase, total protein 및 blood urea nitrogen 함량은 처리구간 차이가 없었으나, AST와 총콜레스테롤 함량은 대조구 (123.75 IU/L, 200.82 mg/dl)가 포도부산물 처리구(95.34 IU/L, 180.63 mg/dl)에 비해 높았다(p<0.05). 이상의 결과에서, 다양한 생리활성을 가진 포도부산물은 젖소를 위한 기능성 사료로 이용 가능성이 높을 것으로 사료된다.