Sangju, a city in the North Gyeongsang Province, is well known as “Three Whites”, representing its three main agricultural products: rice, silkworm, and dried persimmons. Therefore, development of a variety of dishes or products that can promote the consumption of dried persimmons is an urgent requirement for this region. This study was designed to provide fundamental information for revitalizing community-based specialized agriculture through scientific inquiry into medicinal foods and to develop a medicinal food menu based on the results of previous research and pre-survey. The pre-survey results and incompatibility revealed that palatability influenced people’s decision to consume dried persimmons far more than pharmacological functionality. Therefore, palatability played an important part in the development of dried persimmon puddings with acceptable texture. These dishes are in high demand as they suit the tastes of variety of consumers. The inclusion of various additives lowered the sugar content in the developed foods and made the products alkalescent (pH > 7), thereby improving functionality. Unique color and flavor additives were also important criteria that determined the selection attributes of the developed medicinal foods. In addition, the additives had a positive effect on the fragrance and texture of the foods. In conclusion, the results suggested that use of additives provides significant advantages in promoting the consumption of Sangju-dried persimmons by improving both functionality and palatability.

This study aimed to explore how to lead locals to join rural downtown regeneration projects on the basis of the downtown of Obuse Town located in Nagano Prefecture in Japan. What well depicts the outcome of Obuse Town Project joined by residents are revitalized downtown districts and open gardens. Actors that facilitated residents’ participation are administration, "a-la Obuse Co. Ltd. and "Machizukuri Institude of TUS and Obuse Town". "a-la- Obuse Co. Ltd. " is a middle support organization in the form of community business and "Machizukuri Institute of TUS and Obuse Town" is a professional research institute set by Tokyo University of Science and Obuse Town. This study presented activities, types and roles of residents’ participation in the rural town activation project that started in 2015 by researching the activities of residents, shop owners and students with regard to Obuse Town Project, administrative actions for their participation and activities of middle-level supportive institutions, which are exports' research groups.

A pepper bZIP transcription factor gene, CabZIP2, was isolated from pepper leaves infected with an a virulent strain of Xanthomonas campestris pv. vesicatoria (Xcv). Transient expression analysis of the CabZIP2-GFP fusion protein in Nicotiana benthamiana revealed that the CabZIP2 protein is localized in the cytoplasm as well as the nucleus. The acidic domain in the N-terminal region of CabZIP2 that is fused to the GAL4 DNA-binding domain is required to activate the transcription of reporter genes in yeast. Transcription of CabZIP2 is induced in pepper plants inoculated with virulent or avirulent strains of Xcv. The CabZIP2 gene is also induced by defense-related hormones such as salicylic acid, methyl jasmonate, and ethylene. To elucidate the in vivo function of the CabZIP2 gene in plant defense, virus-induced gene silencing (VIGS) in pepper and overexpression in Arabidopsis were used. CabZIP2-silenced pepper plants were susceptible to infection by the virulent strain of Xcv, which was accompanied by reduced expression of defense-related genes such as CaBPR1 and CaAMP1. CabZIP2 overexpression (OX) in transgenic Arabidopsis plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000. Together, these results suggest that CabZIP2 is involved in bacterial disease resistance.

이 연구는 학교 현장에서 쓰레기 줄이기 활동과 관련하여 환경심리학적 강화물이 환경태도 와 실천의식 및 환경 친화적 행동에 어떻게 영향을 미치는지, 그리고 강화물 중 환경 친화적 행동을 증진하는데 가장 효과적인 것은 무엇이며, 환경심리학적 접근이 환경문제를 해결하는데 어떻게 기여할 것인지를 제시하는 것이다. 이 연구를 위해 경기도 소재 J초등학교 6학년 2개 반 70명을 대상으로 실험 수업과 설문 조사를 실시하였고, 그 연구 결과는 다음과 같다. 첫째, 실험 수업 대상 학생들에게 사전⋅사후 검사를 통해 환경 친화적 행동의 변화를 조사한 결과 환경심리학적 강화접근법은 환경태도와 실천의식 및 환경 친화적 행동을 증진시키는데 효과적 인 것으로 나타났다. 둘째, 환경 친화적 행동을 증진시키데 사용한 강화물의 경우 즐거움이 가 장 효과적이었고, 다음으로 쿠폰 혜택, 명예, 현금 순으로 나타났다. 셋째, 환경규범 활성화와 정적⋅부적 강화접근 및 환경 교육은 환경 친화적 행동의 발생빈도를 증가시키는데 기여함으 로써 환경 보호 기능이 가능하다.

본 연구는 햇순나물의 이용성 증진 및 소비 촉진을 위하여 햇순나물을 이용하여 4종의 햇순요리를 개발하고 이들의 관능적 특성 및 영양성을 평가하였다. 햇순요리에 대한 관능평가를 실시한 결과, 4종의 햇순요리의 전반적인 기호도가 3.0 이상으로 만족 수준이었으며, 그 중 '엄나무순닭고기냉채'의 기호도는 5점 만점에 4.48점으로 가장 높은 기호를 보였다. 엄나무 순을 이용한 '엄나무순닭고기냉채'는 단백질, 비타민 A, 비타민 E, 비타민 B6, 엽산의 함량이 권장량보다 높았으며, 비타민 C 및 아연의 함량은 권장량보다 낮았으나 권장량의 50% 이상은 충족하였다. 두릅의 새순을 이용한 '두릅곶감무침'은 곶감과 함께 무쳐 두릅의 쓴맛과 떫은맛을 약화시켰으며, 비타민 E, 비타민 C, 비타민 B6, 엽산 및 철의 함량이 권장량보다 약 1.2~1.7배 정도 높았다. 오가피 순을 이용한 '오가피순꽁치조림'은 무와 오가피순을 첨가하여 조리함으로써 꽁치의 비린내를 줄여 기호도를 높였다. '오가피순꽁치조림'은 단백질 함량이 권장량의 1.5배, 비타민 A, E, C 등 각종 비타민의 함량이 권장량의 1.4~3.6배 함유되어 있었다. 참죽순을 이용한 '참죽만두'는 고기를 넣지 않고 두부와 각종 채소를 넣어 만들었으며, 비타민 및 무기질이 풍부하고 다양한 영양소가 함유되어 있었다. '참죽만두'는 햇순나물 요리 중 가장 열량이 높았으며, 비타민 A, 비타민 E, 비타민 C, 티아민, 인과 철의 함량이 권장량보다 높았다.

We investigated the optimal conditions for keratinase production by feather-degrading Pseudomonas geniculata H10 using one variable at a time (OVT) method. The optimal medium composition and cultural condition for keratinase production were determined to be glucose 0.15% (w/v), beef extract 0.08% (w/v), KH2PO4 0.12% (w/v), K2HPO4 0.02% (w/v), NaCl 0.07% (w/v), MgSO4․7H2O 0.03%, MgCl2․6H2O 0.04% along with initial pH 10 at 200 rpm and 25℃, respectively. The production yield of keratinase was 31.6 U/ml in an optimal condition, showing 4.6-fold higher than that in basal medium. The strain H10 also showed plant growth promoting activities. This strain had ammonification activity and produced indoleacetic acid (IAA), siderophore and a variety of hydrolytic enzymes such as protease, lipase and chitinase. Therefore, this study showed that P. geniculata H10 could be not only used to upgrade the nutritional value of feather wastes but also useful in situ biodegradation of feather wastes. Moreover, it is also a potential candidate for the development of biofertilizing agent applicable to crop plant soil.

The aim of the research was to analyze programs, outcomes, promotion process, and operation methods of neighbourhood making to provide basic information for progressive improvement directions in the future. Buk-gu in Gwanju city, samdeok-dong in Daegu city and dongpirang in Tongyeong city were selected for a comparative analysis. The main bodies of neighbourhood making for promoting a local amenity were analysed for this research. The results indicated that a voluntary and continuous participation of residents was expected in the case of neighbourhood making led by the residents. However, a steady budget support from the outside was necessary for the continuous management and improvement of neighbourhood organization. For this, a voluntary agreement was required through the consultative group and committee formation. The member of civil organization has a limitation in the ability to develop and maintain continuos activities in the case of neighbourhood making led by a civil organization. This research found that operation methods including the constant checking, the search for new ideas through assessment of the resident's participation, local festivals for a community formation and real satisfactions of residents' desires were needed in the neighbourhood making led by a civil organization. A participation rate in neighbourhood making was low in the case of neighbourhood making led by administrative bodies. This result indicated that the long-term strategics for space renewal and community revitalization were important. Various easy ways of residents' participation to reflect their interests and operation systems were also needed. Finally, current local issues, active residents' participation and residents' opinions would be included in the process of neighbour making in the future.

탈모방지 및 발모효과를 가지는 소재를 개발하기 위해 한의학에서 전통적으로 사용되는 23가지 한방소재를 선정하여 한방복합처방단을 개발하였다. 한방복합처방단을 구성하는 한방소재들은 예로부터 전통적으로 발모 및 탈모방지, 흰머리방지, 염증 치료 및 혈액순환개선 효과를 가진 것으로 알려져 있는 당귀, 보골지, 측백엽, 한련초, 구기자, 복분자, 상백피, 숙지황, 여정실, 적하수오, 흑지마, 고삼, 백지, 익모초, 단삼, 도인, 몰약, 감국, 유향, 인삼, 천궁,합환피, 현호색 등이다. 또한, 한방복합처방단의 발모효과를 확인하기 위해 in vitro와 in vivo 평가모델을 이용하여 모발성장 및 촉진에 미치는 영향을 실험하였다. In vitro 상에서는 모유두세포, 각질형성세포 및 섬유아세포의 증식을 확인하였다. 또한, 흰머리방지 효과와 관련하여 멜라노마 세포에서의 멜라닌 합성능력을 확인하였다. 한방복합처방단의 in vitro 상에서의 육모효과는 C57BL/6 마우스를 이용한 in vivo 상에서도 확인하였다. 연구 결과 한방복합처방단은 50 μg/mL의 농도에서 모유두 세포의 증식을 175 %까지, 섬유아세포인 NIH3T3 세포의 증식은 120 %까지 증가시켰으며, 20 μg/mL의 농도에서 각질형성세포인 HaCaT 세포의 증식을 133 %까지 증가시켰다. 멜라닌 합성의 경우, 50 μg/mL의 농도에서 154 %까지 증가시켰다. 또한, C57BL/6 마우스를 이용한 육모효과에 있어서는 한방복합처방단 처리 4주 후 98 % 이상의 육모효과를 나타내는 것을 확인하였다. 이상의 결과로 볼 때 본 연구에서 개발한 한방복합처방단은 모발의 성장 촉진에 유용하게 활용될 수 있는 처방으로 사료된다.

This study was conducted to isolate and characterize a novel feather-degrading bacterium producing keratinase activity. A strain K9 was isolated from soil at poultry farm and identified as Xanthomonas sp. K9 by phenotypic characters and 16S rRNA gene analysis. The cultural conditions for the keratinase production were 0.3% fructose, 0.1% gelatin, 0.04% K2HPO4, 0.06% KH2PO4, 0.05% NaCl and 0.01% FeSO4 with an initial pH 8.0 at 30℃ and 200 rpm. In an optimized medium containing 0.1% chicken feather, production yield of keratinase was approximately 8-fold higher than the yield in basal medium. The strain K9 effectively degraded chicken feather meal (67%) and duck feather (54%), whereas human nail and human hair showed relatively low degradation rates (13-22%). Total free amino acid concentration in the cell-free supernatant was about 25.799 mg/l. Feather hydrolysate produced by the strain K9 stimulated growth of red pepper, indicating Xanthomonas sp. K9 could be not only used to increase the nutritional value of chicken feather but also a potential candidate for the development of natural fertilizer applicable to crop plant soil.

본 연구는 여성 노인들의 운동실천 제약요인과 촉진요인이 연령 단계에 따라 차이가 있는지 확인하고 운동실천 촉진요인과 다차원적 관련이 있는 제약요인이 무엇인지 알아보기 위한 목적으로 수행되었다. 이를 위해 65세 이상 140명의 여성 노인들을 대상으로 심리측정적 타당성 절차를 거친 평정 척도를 통하여 반응을 분석하였다. 다변량분산분석(MANOVA) 결과 여성 노인들의 운동실천 제약요인은 연령 단계에 따라 시간부족과 건강질병 요인에서 차이가 나타났으며, 운동실천 촉진요인은 차이가 없는 것으로 나타났다. 또한 운동실천 제약요인과 촉진요인 간 다차원적 관계를 검증하기 위하여 중다회귀분석(Multiple Regression Analysis)과 정준상관분석(Canonical Correlation Analysis)을 실시한 결과, 운동실천 촉진요인에 관련이 있는 제약요인은 부상우려 요인으로 나타났다. 정준함수식은 1개가 도출되었으며 부상에 대한 공포나 걱정을 의미하는 부상우려가 높을 경우 운동혜택, 운동유발, 운동시간, 사교활동의 운동촉진 요인이 감소하는 것으로 함수 설명력은 13.3%를 나타내고 있다. 이러한 결과를 통하여 부상우려의 제약요인이 여성 노인들의 운동실천 촉진을 가장 방해하는 요인으로 판단할 수 있으며, 여성 노인의 운동실천 촉진에 전반적인 부적 영향을 미치고 있다고 해석할 수 있다.

본 연구는 홍삼 추출기를 이용하여 기능별 사포닌의 함량 및 품질의 최적화 조건을 설정하기 위하여 실시하였다. 홍삼 추출액의 총 사포닌 함량은 75℃ 에서 18시간 추출한 용액이 64.6 mg / 100 ml로 최고 함량을 나타냈고 추출 온도와 시간이 증가할수록 감소하였다. Rb2 와 Re 함량은 75℃ 에서는 12시간 추출한 홍삼액이 11.8 mg / 100 ml로 가장 많았고 추출시간과 추출온도가 증가할수록 급격히 감소하였다. Prosapogenin 함량은 85℃ 에서 24시간 추출한 홍삼액이 34.9 mg / 100 ml로 가장 많았고, 75℃ 와 85℃ 에서는 추출 시간이 길어짐에 따라 증가하는 경향이었으나 95℃ 에서는 감소하는 경향이었다. 홍삼 추출액의 총 당은 95℃ 에서 추출시간을 길게 할수록 함량이 증가하였고, 당도는 95℃ 에서 24시간 추출한 용액이 4.0%로 가장 높았다. 탁도는 95℃ 에서 추출시간이 길수록 증가하였으며, pH 및 Hue 값은 추출시간이 길어질수록 낮아졌다. 따라서 홍삼추출액을 제조할 때 1차 추출에서는 온도를 낮게하여 사포닌의 함량을 높이고, 2차 추출에서는 온도를 높게하여 맛을 증진시켜 혼합하는 방법이 좋을 것으로 생각된다.

The purpose of this study is to examine the aspects and the characteristics of conflict among people in agricultural villages shown in the course of conducting a rural community development project, and to suggest a theoretical basis for the solution of the conflict. various theories on rural community development projects and conflict, and relevant sociopsychological theories were perused to lay a theoretical foundation for the study, and 5 actual cases of conflicts in agricultural experience villages were analyzed in the light of the grounded theory which is one of suitable methods of qualitative research. And comparative and comprehensive analysis of conflicts of different farm villages was done. Based on the result of analysis of conflict among residents on agricultural experience village, this paper suggests some ways for solution of the conflict.

The promotion of walking and bicycling is recently a hot topic in the urban planning and design field. Many planners have already examined the many components of the land use-transportation connection and built environment-physical activity link. A rapidly growing area of urban form research is to measure the level of walkability in urban environments. With this background, this research conducted a preliminary study to develop the evaluation indicators of pedestrian environments. Based on the literature reviews on walking or pedestrian environments, we proposed the seventeen indicators related with pedestrian facilities, road attributes and walking environment. We also performed a questionary survey to evaluate the satisfaction of their neighborhood pedestrian environments for 302 randomly selected adults living in the City of Changwon, Gyeongsangnam-do. Finally, this research provided the valid model to evaluate the effects of physical environmental factors on the walking satisfaction using factor analysis and multiple regression analysis.

The purpose of this study was to show the effect of psychological-horticultural therapy for promoting ecmotional intelligence. We carried out the program which was designed for promoting emotional intelligence and subjects were 11 children at K welfare center in Jungrang-gu. Children were tested before and after the program and the program applied for 8 weeks. Emotional intelligence was measured before and after treatment. We used the quantitative and qualitative analysis method and data were analyzed by wilcoxon t-test using SPSS 12.0. The score of emotional intelligence were increased slightly after the program, but it was not significant. In qualitative viewpoint, children were changed in perception and expression of emotion and empathy and they experienced.