In a previous study, we fractionated crude polysaccharide (AME-CP) with macrophage-stimulating activity from a hot-water extract (AME) of Astragalus membranaceus. AME-CP contained glucose (Glc) as a main component sugar, suggesting that it might be rich in starch-like compounds (SLC). To enhance the immunostimulating activity of AME-CP by pruning SLC rarely known to contribute to activity, hydrolysate (AME-SH) was prepared by digesting with starch-related enzymes, including α-amylase and amyloglucosidase. AME-SH was found to contain substances with molecular weights ranging from 3.9 to 84.4 kDa. These substances were primarily composed of galactose, galacturonic acid, Glc, arabinose, rhamnose, and mannose. AME-SH significantly enhanced the production of macrophage-stimulating factors, including nitric oxide (NO), interleukin (IL)-6, and IL-12, in RAW 264.7 cells compared to AME-CP. Treatment of splenocytes isolated from C3H/HeN mice with AME-SH not only promoted IL-6 secretion, but also induced mitogenic activity. In addition, AME-SH promoted the secretion of hematopoietic growth factors including IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) in Peyer's patch (PP) cells and stimulated bone marrow cell proliferation through these PP cells. In conclusion, hydrolysate (AME-SH) digested from AME-CP with starch-related enzymes could be used as a potential immunostimulant.

This study presents an analysis of bycatch data concerning Pacific white-sided dolphins (Lagenorhynchus obliquidens) along the Korean coast from 2016 to 2021. A total of 503 bycatches were examined, encompassing data on year, month, body length, sex, latitude (N), longitude (E), and fishing gear. Bycatch was the most frequent in waters with a longitude of less than 130°E, particularly in the southern coastal region at a latitude of 35.5°N, with a higher likelihood of bycatch in lower latitudes. Since 2017, a decreasing trend in bycatches has been observed. The sex distribution of bycaught dolphins showed a predominance of males (40%), followed by females (31%), with an unclassified category at 29%, though no statistically significant differences were found (p > 0.05). Seasonal analysis indicated that bycatch predominantly occurred during the winter months, with significant monthly variations (p < 0.01). Pacific white-sided dolphins were primarily ensnared in gill nets and, to a lesser extent, in stationary nets. Statistical analysis by gear type revealed a significant preference for gill nets (p < 0.001). Considering body length composition in relation to latitude, it is suggested that Pacific white-sided dolphins may utilize the southern waters of the East Sea as a potential nursery ground, though this was not confirmed at a significant level, emphasizing the need for further in-depth monitoring and ecological investigations. Given that there are approximately 27 different types of gill nets associated with the majority of bycatches, more detailed research is warranted to divide these gear types into finer subcategories for estimating bycatch relationships, ultimately leading to the development of effective conservation and management strategies.

The United States enforces the seafood import regulations so-called the Marine Mammal Protection Act (MMPA), and by 2023, all exports of aquatic products and processed fish products by fisheries which have not obtained an “Comparability Finding” from the National Oceanic and Atmospheric Administration will be completely banned. Therefore, to respond to the US MMPA, it is critical to identify technologies and methods used in worldwide for reducing bycatch of marine mammals. In particular, marine mammals are frequently caught in five fisheries (trawl, gill net, trap, stow net and set net) in Korea, which is facing a great challenge. This study presented bycatch reduction methods by five fisheries, classified the methods by country, and suggested appropriate reduction methods which can be applied in Korea.

This study investigated the species composition and bycatch status of fishes by fyke net between June and November 2020 in Asanho lake and Chungjuho lake. During the survey period, the catches in Asanho lake were identified as 2,931 individuals of 16 species from a total of six families and in Chungjuho lake were identified as 1,645 individuals of 14 species from a total of eight families. The dominant species in Asanho lake was Erythroculter erythropterus which accounted for 59.6% of the the total number of individuals and 40.5% of the total biomass caught. The dominant species in Chungjuho lake was Lepomis macrochirus which accounted for 48.2% of the the total number of individuals and Erythroculter erythropterus which accounted for 60.2% of the total biomass caught. The bycatch rates of non-commercial species in Asanho lake and Chungjuho lake were 77.0% and 82.6%, respectively. As a result of analyzing the frequency distribution of the total length of Carassius auratus and Siniperca scherzeri, which were the dominant species among commercial species, the bycatch rate of small individuals was 87.1%, and 42.7%, respectively.

Maca roots (Lepidium meyenii) are an important medicinal herb that have long been used by the Andes-indigenous peoples and South Americans. In Korea, recently, it has attracted attention as a health food material because of nutritional values and physiological activities. The purpose of this study was to investigate the industrial applicability of maca (red and golden varieties; R&G) as immunostimulating materials. In the macrophage stimulating assay using RAW 264.7 cells at 125~500 μg/mL of non-cytotoxicity doses, G-HW showed the most potent production of TNF-α, IL-6 and nitric oxide compared to red maca or the other extracts. The general component analysis results showed that all extracts comprised more than 90% neutral sugars with small amounts of uronic acid and protein. Meanwhile, component sugar analysis showed the difference in the content of uronic acids of cold- and hot-water extract. Additionally, the further fractionation of G-HW into crude polysaccharide (G-CP) greatly enhanced the macrophage stimulating activity, and G-CP contained macromolecules over 144 kDa, comprised mainly of glucose and galacturonic acid (51.0 and 34.9%). In conclusion, crude polysaccharide from maca showed industrial applicability as immunostimulating material, and especially golden maca showed higher macrophage stimulating activity than red maca.

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.

The species composition and abundance variations of fishery resources in the adjacent marine ranching area, Tongyeong, Korea, were investigated by shrimp trawl, gill net, and longline during the period of July, September, and October in 2016. During the study period, the total catch were 8,522.9 kg with 34 species from the shrimp trawl, 32 species from the gill net, and nine species from the longline. The dominant species were different by gear, which were Hypodytes rubripinnis and Parapercis sexfasciata in the shrimp trawl, Platycephalus indicus and Raja kenojei in the gill net, and Conger myriaster and Scomber Japonicus in the longline. In terms of spatial distribution, Yongchodo showed the highest total catch in number as well as of in weight while Jukdo showed the lowest total catch in number and Bijindo showed the lowest total catch in weight. The amount of demersal fish resources in the survey area estimated as 301 ha, was 99,396 individuals which was converted to be 8,552.9 kg. The amount of demersal fish resources by gear were of trawling area, which area is 127 ha, were 76,251 and 3,489.5 kg, 74 ha in the gill net and longline survey area was 16,213 and 3,457.3 kg, and the other 100 ha area was 6,932 and 1,606.1 kg. In this study, the minimum resources for demersal fish is 61,687 and 4,265.2 kg, and the maximum is 149,439 and 14,197.9 kg.

This study was conducted to analyze fishing capacity and bycatch by mesh size and entrance size of spring net pot conductedby water tank and field experiment. The water tank experiments were conducted by using traps with mesh size of 22 mm and entrance size of 120 mm and 140 mm, respectively in the water tank of NIFS. The field experiment was conducted using 5 kinds of spring net pot with mesh sizes of 20 mm, 22 mm, 35 mm and entrance size of 120 mm, 130 mm, 140 mm, 360 mm by coastal trap fishery vessel operating around the area of Geoje island. In the result of water tank experiments, the catch of conger eel was 1.5 times higher when using trap with entrance size of 140 mm than that of 120 mm. In the field experiment, when using same mesh size, the larger the entrance size, the higher amount of conger eel catch, bycatch and number of bycatch species. When using the same entrance size, the larger the mesh size, the lower amount of conger eel catch and number of bycatch species, whereas the amount of bycatch showed increasing trend.

Species composition and bycatch rate of the catches by dredge with bycatch reduction device to reduce bycatch were estimated around Yeongil Bay, Korea at June, 2015. Total catches were collected 31 species as Chordata 10 species, mollusca 8 species, arthropoda 6 species, echinodermata 5 species, sipunculida 1 species, annelida 1 species during the experiment at the Yeongil Bay. The dominant species were Scapharca broughtonii, Callithaca adamsi, and Atrina pectinata and they reached 79.9% of the total catch. The catch rate of Scapharca broughtonii and Callithaca adamsi was 69.6% and 10.4% respectively. The rate of bycatch was 20.1%, consisted with Echinodemata 13.0%, Chordata 3.6%. The catch of ordinary dredge was 27 species 1,307 number 85,052g and that of dredge with escapement device was 21 species 1,690 number 91,648g. In conclusion, the dredge gear with bycatch reduction device has higher catch rate of target species and lower bycatch rate to non–target species than ordinary one.

The 1/5 scale–down model of the Bycatch Reduction Device (BRD) from an Argentinean demersal trawl was tested in a circulating water channel. The BRD is designed to help small Hake (merluza, merluccius hubbsi) to escape from a trawl. It is settled in front of a trawl codend, and is equipped with selection grids that help small fish to escape from the gear and guiding panels that help fish to meet with the grids. Bars of the grids are wires covered by the PVC and other parts of the BRD are made of net. When the velocity was less than 0.65 m/sec (2.81 Kont when translated to real towing speed) which is slow speed compared with real towing speed, position between an upper guiding panel and an upper selection grid were good to help small fish to escape. When the velocity was more than 0.8 m/sec (3.41 Knot when translated to real towing speed) which is similar to and faster than real towing speed, it was considered that small fish may have difficulties in escaping because the gap was not enough between an upper guiding panel and an upper selection grid. The lower selection grid was sat on the bottom of the tank without an angle due to the weight that it carries. Improvements were proposed to position the panels and the grids better.

Experiment was set up to analyze bycatch and discards situation including snow crap Chionoecetes opilio of whelk trap. Four types of trap were used: drum type trap with PE net; drum type trap with PBS net; cylinder type trap with PE net; and cylinder type net with PBS net. Three funnels were attached in drum type trap and two funnels were used in cylinder type trap. A fleet of traps was consisted with one hundred traps. 25 traps of each type were set on a line in repeated sequence. Field experiments were conducted with 6 fishing operations in the Uljin waters, East Sea in July 2014. Catch of target whelks were 173,261 g and catch rate was 48.7% of total catch, while bycatch were 182,571 g, 51.3% of tatal catch. The catch rate of bycatch was 2.6% higher than that of target catch. Bycatch weight of snow crap was the highest as 142,987 g and formed about 40.2% of total catch, followed giant octopus, Enteroctopus dofleini, 31,762 g (8.9%). Bycatch rate of cylinder type trap was 2.3 times higher than that of drum type trap. Discard rate (discard/(discard+landing)) was 43.6%. Discard rate was the highest at cylinder type trap with PBS net as 63.1%, followed cylinder type trap with PE net as 47.9%, drum type trap with PE net as 33.4%, the lowest at drum type trap with PBS net as 22.1%.

본 연구는 산지형 근린공원 시설지의 생태경관적 식재계 획으로 서울시 초안산근린공원에 기조성된 시설지 3개소에 대하여 시설지와 유역권의 지형, 수계, 현존식생 등 입지조 건을 조사하였고 공간기능과 식재개념, 배식구조를 분석하 여 공간별 불일치하는 지역과 수목 생육상태가 불량한 지역 을 파악하였다. 부적합한 공간을 대상으로 개선방향, 식재 개념, 입지유형별 식재구조 모델을 설정하여 주변 산림과 조화로운 경관향상과 생태적으로 안정된 생태경관적 식재 계획을 제안하였다. 도봉문화정보센터 동측 시설지(대상지 Ⅰ)는 능선부에 조성된 13,060㎡의 시설지로 0°~20°의 평지 및 완경사지가 86.7%이었으며 음지에 해당하는 북, 서향이 62.0%로 일조 량이 부족하였다. 유역권 내 현존식생은 아까시나무가 46.7%이었으며 우점식재종은 산딸나무, 상수리나무, 벚나 무, 은행나무, 소나무로 유역권 내 현존식생과 우점식재종 이 대부분 불일치하였다. 수계 영향, 일조량, 지형 등의 영향 으로 생육이 불량한 식재지역은 39.5%이었다. 정밀조사구 는 9개소를 설정하였으며 조사결과 평균녹피율은 50.60%, 평균녹지용적계수는 1.21m³/m²이었다. 대상지 Ⅰ은 대부 분 경관공간(47.9%)임에도 특별한 주제 없이 교목층 단층 구조(74.7%)로 식재되어 있었고, 공간기능과 식재개념, 배 식구조가 불일치하는 지역은 3,595㎡(식재면적의 35.9%) 로 분석되었다. 창동 주공4단지 동측 시설지(대상지 Ⅱ)는 계곡부와 사 면부에 조성된 8,468㎡ 면적의 시설지로 0°～20°의 평지 및 완경사지가 97.3%이었으며 음지에 해당하는 북, 서향이 54.2%로 일조량이 부족한 것으로 파악되었다. 유역권 내 현존식생은 아까시나무림 40%, 상수리나무림은 11.3%, 신 갈나무림이 1.0%이었으며 우점식재종은 스트로브잣나무, 살구나무, 단풍나무, 벚나무로 유역권 내 현존식생과 대상 지 내 우점식재종이 불일치하였다. 수계, 지형 등의 영향으 로 수목생육이 불량한 지역이 64.7%이었다. 정밀조사구 7 개소 조사결과 평균녹피율은 38.90%, 평균녹지용적계수는 0.7m³/m²이었다. 대상지 Ⅱ는 녹음공간(10.0%)보다는 경관공간(51.2%) 과 완충공간(21.8%)이 필요한 지역이었으나 특별한 주제 없이 교목층 위주의 단층구조(80.6%)로 식재되어 있었고 공간기능과 식재개념, 배식구조가 불일치하는 지역은 3,876m²(식재면적의 55.1%)로 분석되었다. 창골어린이공원 시설지(대상지 Ⅲ)는 계곡부와 사면부에 조성된 5,917㎡ 면적의 시설지로 0°～20°의 평지 및 완경 사지가 83.7%이었으며 음지에 해당하는 북, 서향이 85%, 평지 14.2%로 일조량이 매우 부족한 지역이었다. 유역권 내 현존식생은 아까시나무림이 53%이었으며 우점식재종 은 잣나무, 아까시나무, 느티나무, 은행나무로 현존식생과 대상지 내 우점식재종이 불일치 하였다. 수계, 일조량 부족 등으로 인하여 생육이 불량한 지역이 25.1%이었다. 정밀조 사구는 9개소를 설정하였으며 조사결과 평균녹피율은 123.88%, 평균녹지용적계수는 3.53m³/m²이었다. 대상지 Ⅲ은 산림주연부와 인접하여 완충기능(38.1%)과 경관기능(29.0%)이 중요한 지역이었으나 대부분 교목층 위 주의 단층구조(99.8%)로 식재되어 있었고 공간기능과 식재 개념, 배식구조가 불일치하는 지역은 2,973m²(식재면적의 64.2%)로 분석되었다. 시설지 식재계획은 개선방향과 공간기능에 따라 적정 식 재개념을 설정하고 대상지와 유사한 입지조건을 가진 능선 부, 계곡부, 사면부 등 입지 유형별 식재구조 모델을 도입하 였다. 경관공간은 각 수목의 경관적 특성을 고려하여 다층 구조 식재로 다양한 경관을 연출하도록 하였고 완충공간을 공간 내부측 경관을 고려하여 지하고가 낮고 지엽이 치밀한 낙엽활엽수의 다층구조 식재를 제안하였다. 산림주연부 인 접 완충지역은 자생종을 선정하여 공원 내부에서부터 초본 층-관목층-아교목층-교목층 순으로 배식하여 산림주연부가 단절되지 않도록 계획하였다. 본 연구는 서울시 도봉구에 위치한 산지형 공원인 초안산 근린공원 시설지를 대상으로 유역권과 대상지의 입지조건 을 분석하였고 공간기능과 식재개념, 배식구조를 조사하여 공간별 불일치하는 지역과 수목 생육상태가 불량한 지역을 입지유형별 식재구조 모델을 설정하여 주변 산림과 조화롭 고 생태적으로 안정된 식재계획을 제안하였다.

As a basic study to improve hooking ability of long line fishing gear, which is widely used around Jeju-do coast, the researcher performed hooking experiment of parrot fish by manufacturing and installing 7 kinds of model long line fishing gears, whose thickness of branch line are different and 8 kinds of model long line fishing gear, whose length of branch line are different, in indoor circular aquarium, which is installed for the model experiment of thickness and length of branch line that are various by fishing implement and improper. The hooking rate depending on thickness and length of branch line was calculated and the effect of thickness and length of branch line on hooking rate was analyzed. Its results are as follows. When branch line was thin and long, high hooking rate appeared. In the scope of value setting, the relationship between thickness (Bt) of branch line and total hooking rate (Thr) can be shown as following formula as. In the scope of value setting, the relationship between length (Bt) of branch line and total hooking rate (Bt) can be shown as Thr=-20.83Bt+26.04. Through Pearson correlation analysis, the coefficient of correlation between thickness of branch line and hooking rate was -0.718. Therefore it showed significance in 0.01 significance level. Through Pearson correlation analysis, the coefficient of correlation between length of branch line and hooking rate was 0.431. Therefore it showed significance in 0.01 significance level.

Experimental fishing was carried out to compare the catches of main species and bycatch according to the codend mesh size (raschel, 15mm, 25mm, 35mm, 45mm) of stow net on anchor in the coastal waters of Seocheon after manufacturing five kinds of fishing gears and commercial fishing boat (7.93 tons) was used for it. Anchovy (Engraulis japonicus), southern rough shrimp (Trachysalambria curvirostris), beka squid (Loligo beka), cuttle fish (Sepia esculenta), swimming crab (Portunus trituberculatus) were dominant on the five kinds of experimental fishing gears. Southern rough shrimp was caught from all kinds of fishing gears and catches of anchovy was highest in the raschel codend, southern rough shrimp for 15mm, 25mm, 35mm codend and beka squid for 45mm codend. Bycatch proportion against total catches was 623,376g (28.7%) for 2,171,844g in raschel, 11,932g (1.7%) for 704,451g in 15mm, 3,616g (1.6%) for 221,432g in 25mm, 9,276g (4.9%) for 191,052g in 35mm and 2,724g (5.3%) for 51,431g in 45mm.

This investigation was carried out to research on the actual states for bycatch and discards of catches caught by funnel net from December, 2007 to June, 2010. Fishing grounds were Manheung-dong and Ocheon-dong in the coastal waters of Yeosu. The number of the investigation was 21 in Manheung-dong and 7 in Ocheon-dong fishing ground. In Manheung-dong, the species of catches were 26 of fish, 6 of cephalopod and 5 of crustacean. In Ocheon-dong, the species of catches were 25 of fish, 6 of cephalopod and 4 of crustacean. In the two all fishing grounds, the largest amount of species was revealed as gray mullet. Among these catches, the number of non marketable species that were classified and discarded ones, were 9 in Manheung-dong and 7 in Ocheon-dong. Also, among the marketable species which were small entity or too tiny catches to commercialize and not fresh ones were discarded. The proportion that they dominate in the whole catches was 37.1% in the number of catches and 5.4% in the weight of catches in Manheung-dong, and 6.9% in the number of catches and 0.3% in the weight of catches in Ocheon-dong. In case of Manheung-dong, the monthly discarding proportion was the highest with 59.4% in the number of catches in may and 17.6% in the weight of catches in November, and in case of Ocheon-dong, the both of them were the highest in February, with 28.0% in the number of catches and 5.1% in the weight of catches.

Test fishing was carried out using six kinds of different mesh sizes (20, 22, 24, 28, 35, 40mm) for springnet-pot to study bycatches according to the mesh size and catches survey was done for another one (mesh size : 22mm, entrance round : 350mm) in Geo-je & Tong-young waters of Korea. On the first sea experiment, it was thought that suitable mesh size of spring-net-pot catching conger-eel over 35cm with decreasing the catches of conger-eel (Conger myriaster) below 35cm was 24mm. On the second sea experiment, commercial catches were crabs (Charybdis bimaculata), octopus minor (Octopus variabilis) and others including conger-eel, and catches proportion was 60% of total catches weights. There was no big difference for the monthly catches. Self-consumption catches were 9 species including conger-eel below 35cm holding 50% of catches in the side of weights. There were 40% of bycatches for the catches weights and 63% for catches numbers in the 22mm mesh size of spring-net-pot having entrance round over 140mm. It showed that 50% of catches weights were discarded.

For an effective management of fisheries resources, we need fisheries informations necessary for the establishment of reasonable fishing effort and TAC distribution. To study the present state of by-catch and discard of gill-net fishery in the East Sea, we carried out fishing research using trammel nets in the coastal sea of Susan-port (Yangyang), Jangho-port (Samcheok) and Hupo-port (Uljin). As a result, a total of catch of Susan was 259,172g and that of Hopo was 577,638g. Thus Catch of Susan was 1.49 times more than that of Hupo considering the number of used fishing gear. And catch of Janho was 7.4 times more than that of Susan considering the number of experiment. Two methods of by-catch analysis in this study were used, one method is standardizing of listed species catching possible in fishing license, another method is regarding catch species under 1% of total catch as by-catch. As a result of by-catch analysis of two method, by-catch rate of Susan was 6.55% and 5.87% respectively, that of Hupo was 44.70% and 5.24% and that of Jangho was 0.96% and 2.31%. Discard rate of fish in Susan, Hupo and Jangho were 1.59%, 3.42%, 2.23% respectively.