The objective of this study is to evaluate the antioxidant components, elastase inhibition activities, and melanin synthesis rates of Korean traditional Actinidia (Actinidia arguta) fruits and leaves depending on the ethanol extraction concentrations. The total polyphenol content was the highest in the 50% ethanol extract of both fruits and leaves, with values of 634.1 mg GAE/100 g and 3,985.2 mg GAE/100 g, respectively. The total flavonoid content was the highest in the fruit 90% extract and leaf 50% extract at 191.9 mg/100 g and 2655.6 mg/100 g, respectively. The vitamin C content was the highest in the 50% extract of leaves at 2990.3 mg/100 g. Elastase inhibition was the highest at 56.9% in the leaf 50% extract at a concentration of 1,000 μg/mL. Melanin synthesis inhibition showed the highest melanin synthesis inhibitory effect among the extracts, as the leaf 50% extract showed an inhibitory rate of 65% or more. Therefore, the antioxidant components, elastase inhibition activities, and melanin synthesis inhibitory rate were better in leaves than in fruits. The leaf 50% extract was particularly the best among the extracts. Korean traditional Actinidia leaves can be considered as potential sources for new functional materials.

The annual consumption of kiwifruit in Korea is about 50,000 tons, and the gold-fleshed cultivar has increased to about 30% among cultivated ones, but the green-fleshed kiwifruit is still the most at about 60%. The green-fleshed kiwifruit, which was introduced in the late 1970s, is relatively sour and tasteless for consumers compared to the gold-fleshed one. This acts as an obstacle to the increase in domestic kiwifruit consumption. Therefore, to promote kiwifruit consumption by breeding green-fleshed kiwifruits that are not sour and are delicious. ‘Garmrok’, bred in 2013, is a green-fleshed variety that was officially registered as No. 6038 according to the Plant Variety Protection Act by the Commissioner of the Korea Seed and Variety Service in 2016, and it is being distributed through contracts with the Rural Development Administration (RDA). Field crossing was conducted in 2003 at the Namhae branch of the National Institute of Horticultural and Herbal Science, and the maternal parent was germplasm collection IT233199 (Actinidia deliciosa) introduced in China, and the paternal parent was germplasm collection IT233198 (A. deliciosa) also introduced in China. The 2003-3-152 line with high soluble solid content (SSC) and good taste was first selected in 2009, and it was finally selected in 2013 by a detailed characteristic observation and evaluation through trunk-graft proliferation. It is a variety with less sour taste and higher sweetness compared to the existing dominant green-fleshed cultivar ‘Hayward’. It weighs 80 to 120 g, has a SSC of 14 to 18 °Brix, and has a low acid content of 0.3 to 0.7%. The full blooming is May 15-20, and the harvest season is 160-170 days after anthesis, which is expected to relieve the risk of frost damage during the harvest season.

참다래 열매로부터 분리한 균주의 형태학적 특성과 DNA를 분리하여 효모의 동정에 사용되는 특이적 프라이머로 PCR 증폭을 한 후 생성된 PCR 산물의 염기서열 분석 결과, S. cerevisiae로 동정하였고, 동정된 균주는 S. cerevisiae HKFR18로 명명하였다. HKFR18을 적용하여 병행복발효, 단행복발효 및 단발효를 실시한 결과, 병행 복발효 산업에서 사용되는 상업용 효모 S. cerevisiae La parisienne 대비 발효제로 개량누룩 사용 시험구의 경우, 동등 수준의 알코올 생성능을 나타내었고, 신맛의 특성과 강도에 차이를 나타낼 수 있는 유기산 조성의 차이가 확인되 었으며, 입국 사용 시험구의 경우에는 알코올 생성능, 유기산 조성 및 향기성분 조성에서도 특이적인 차이는 관찰 되지 않았다. 단행복발효 산업현장에서 사용되고 있는 상업용 균주인 S. cerevisiae US-05 시험구 대비 HKFR18 시험구에서 동등 수준의 알코올 생성능이 확인되었고, 총 유기산 함량은 동등 수준이지만 조성에서는 차이가 확인 되었으며, 향기성분 조성도 유의적인 차이를 나타내는 성분들이 검출되었다. 단발효 산업현장에서 사용되고 있는 상업용 균주인 S. cerevisiae Fermivin 시험구 대비 HKFR18 시험구에서 동등 수준의 알코올 생성능과 총 유기산 함량은 동등 수준이지만 조성에서는 상대적으로 acetic acid 함량이 낮은 특성, 검출된 향기성분 대부분 높은 함량을 나타내었다. 이와 같이 국내 자생 S. cerevisiae 균주인 S. cerevisiae HKFR18이 주류 산업 현장에서 적용 되고 있는 상업용 S. cerevisiae 균주의 대체 가능한 주류 양조 가능성이 확인되었으므로 HKFR18과 상업적으로 이용되고 있는 S. cerevisiae 균주와의 관능품질 차별화 및 계통군 분류에 대한 심화 연구를 통하여 국내 자생 생물자원의 활용 가능성을 제고할 수 있는 단서를 제공할 수 있 을 것으로 생각된다.

본 연구는 헤이워드 다래나무의 비정상 생장 관련 문제점을 파악하고자, 건전 개체와 비건전 개체 간의 물리·해부학적 특성 및 Calcium Oxalate 형성 특성을 비교분석 하였다. 두 집단 간 밀도와 함수율 등의 물리적 요인과 연륜폭, 도관직경, 도관비율, 방사조직 높이와 너비 등의 해부학적 특성 요인을 비교분석해 본 결과 모든 요인에서 유의적인 차이가 나타났다. 특히 Calcium oxalate 결정은 주로 수피 부분에 여러 개가 다발형태로 다수가 존재하였으나, 도관 주위에서는 출현율이 낮았고, Pith와 목질부에서는 거의 나타나지 않았다. 이러한 Calcium oxalate 결정체는 건전개체(9.0 ×106/g개) 보다 고사가 진행 중인 비건전개체(10.4×106/g)에서 유의적으로 출현빈도가 높게 나타났음을 알 수 있었다.

Yield and fruit size are under the control of many interacting factors and crop failures can be caused by climatic extremes and poor flowering. Nevertheless, this study has shown that mulching, through the creation of improved soil properties and reduced environmental stress, has the potential to substantially increase A. arguta fruit size and yield. The mulched site performed better than the site without mulch in reducing soil temperature, increasing soil moisture and controlling available phosphorus. Moreover, mulching improved both yield and fruit size. In light of this, we hope that the results of our research will provide useful information for the cultivation of A. arguta in South Korea. However, it also seems necessary to conduct research on the use of different types of mulch and on the economic profitability of mulch in the future.

Soil properties are important environmental conditions affecting fruit quality and yield in new cultivars of Actinidia arguta. The yield of three A. arguta cultivars (cv. Autumn Sense, cv. Chungsan, and cv. Greenball) from Wonju city, South Korea was investigated from 2017 to 2019 and the relationships to soil properties are discussed. The yield of cv. Autumn Sense, cv. Chungsan, and cv. Greenball fruits ranged from 6.8 to 24.5, 14.0 to 29.0, and 10.5 to 38.5 kg/vine. cv. Autumn Sense had the highest soil organic matter content and soil C/N ratio over the three-year period. The yield of A. arguta fruits was positively correlated with soil C/N ratio and could be described by a power model (y = axb), suggesting that soil C/N ratio plays an important role in limiting the bioavailability and bioaccumulation of organic matter in A. arguta fruits. In addition, C/N ratio in the soil was influenced by the soil available phosphate. However, the threshold of the C/N ratio for A. arguta fruit differed according to cultivar, and especially the lowest threshold was observed in cv. Chungsan. Therefore, the application of an appropriate C/N ratio depending on the cultivar is required for improved fruit yield. Our results present the soil properties required to increase the yield of the new A. arguta cultivars in South Korea.

Environmental conditions are important in increasing the fruit sugar content and productivity of the new cultivar Autumn Sense of Actinidia arguta. We analyzed various soil properties at experimental sites in South Korea. A Pearson’s correlation analysis was performed between the soil properties and sugar content or productivity of Autumn Sense. Further, a decision tree was used to determine the optimal soil conditions. The difference in the fruit size, sugar content, and productivity of Autumn Sense across sites was significant, confirming the effects of soil properties. The decision tree analysis showed that a soil C/N ratio of over 11.49 predicted a sugar content of more than 7°Bx at harvest time, and soil electrical capacity below 131.83 μS/cm predicted productivity more than 50 kg/vine at harvest time. Our results present the soil conditions required to increase the sugar content or productivity of Autumn Sense, a new A. arguta cultivar in South Korea.

In this study, we tried to find out the most appropriate pre-processing method and to verify the feasibility of developing a low-price sensing system for predicting the hardy kiwis sugar content based on VNIRS and subsequent spectral analysis. A total of 495 hardy kiwi samples were collected from three farms in Muju, Jeollabukdo, South Korea. The samples were scanned with a spectrophotometer in the range of 730-2300 nm with 1 nm spectral sampling interval. The measured data were arbitrarily separated into calibration and validation data for sugar content prediction. Partial least squares (PLS) regression was performed using various combinations of pre-processing methods. When the latent variable (LV) was 8 with the pre-processing combination of standard normal variate (SNV) and orthogonal signal correction (OSC), the highest R2 values of calibration and validation were 0.78 and 0.84, respectively. The possibility of predicting the sugar content of hardy kiwi was also examined at spectral sampling intervals of 6 and 10 nm in the narrower spectral range from 730 nm to 1200 nm for a low-price optical sensing system. The prediction performance had promising results with R2 values of 0.84 and 0.80 for 6 and 10 nm, respectively. Future studies will aim to develop a low-price optical sensing system with a combination of optical components such as photodiodes, light-emitting diodes (LEDs) and/or lamps, and to locate a more reliable prediction model by including meteorological data, soil data, and different varieties of hardy kiwi plants.

본 연구는 과육색이 노란 국내 육성 키위 ‘Jecy Gold’ 품종의 수확 적기 판정과 후숙 후 당도 예측 방법을 개발하고자 수행하였다. 과실 생육 단계 중 과실 비대기는 BBCH 70~78(DAA 10~110), 과실 성숙 단계는 BBCH 80(DDA 140), 당도가 급격히 증가하는 시기는 BBCH 83(DDA 160), 과육이 부드러워지는 시기는 BBCH 87(DAA 190), 과실이 노화되는 시기는 BBCH 90(DDA 210)이었다. 이러한 결과를 토대로 BBCH 83(DAA 160일)에서 수확하는 것이 가장 바람직할 것으로 판단되었다. 후숙 전 과실 건물률과 후숙 후 당도가 정의 상관관계를 보였다. 후숙 전 건물률을 이용하여 후숙 후 당도를 예측하기 위하여 회귀분석을 수행한 결과, 후숙 후 당도(y)=1.1841ⅹ건물율(x)-3.5244(r²=0.9338)으로 나타났으며, 실측 값과 예측 값을 이용하여 회귀분석을 수행한 결과, 상관계수 R² 값이 0.86으로 높게 나타났다. 따라서 수확 시기의 건물률을 이용하면 후숙 후 당도를 추정할 수 있을 것으로 판단된다.

Actinidia arguta (Actinidiaceae), which is commonly referred to as hardy kiwifruit, has been reported to possess anti-inflammatory, anti-allergic and antioxidative properties. The protective effect of the leaves and stems of A. arguta against amyloid β protein (Aβ) (25-35)-induced cultured neuronal cell death and memory impairment was investigated in the current study. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 24 h induced significant neuronal death as assessed by a 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. However, A. arguta (10 and 50 μg/ ml) prevented Aβ (25-35)-induced apoptotic neuronal death in cultured cortical neurons. A. arguta also inhibited the 100 μM H2O2-induced decrease of the MTT reduction rate in cultured neurons. Memory impairment was produced by intracerebroventricular microinjection of 15 nmol Aβ (25- 35) and examined using the passive avoidance test in ICR mice. Chronic treatments with A. arguta (50 and 100 mg/ kg, 14 days, p.o.) significantly prevented memory impairment induced by Aβ (25-35), and A. arguta inhibited the Aβ (25-35)-induced increase of cholinesterase activity in the brains of memory impaired mice. These results suggest that A. arguta might be able to inhibit Aβ (25-35)-induced neuronal death and memory impairment via antioxidative and anti-cholinesterase effects and that A. arguta could have a therapeutic role for preventing the progression of neurodegeneration in Alzheimer’s disease.

참다래(Actinidia chinensis)의 연화과 발생은 재배 환경과 기후의 영향을 받기 때문에 경작년에 따라 발생 정도에 차이가 있다. 수확전 키토산 살포는 황육계 및 적육계 참다래 과실의 경도를 증가시키는 것 으로 확인되었지만 수확후 침지처리는 유의한 결과를 보이지 않았다. 수확전 키토산 살포는 과실 성숙을 균일하게 만들어 주어 적육계 참다래의 수확기 연화과 발생율을 감소시켰다. 과실내부 조직의 CO2 농도 는 대조구에 비하여 45% 증가하였는데 이는 과피에 키토산 피막이 형성되었기 때문이었다. 수확전 처리 한 키토산에 의하여 과실 건물중과 전분함량이 증가하였고 가용성고형물과 산함량이 높아져 과실 성숙이 늦어졌으며 저장 중에도 과실 경도 및 전분 감소가 지연되었다. 수확전 키토산 처리는 적육계 참다래의 과피 반점 형성을 감소시켜주었다. 결론적으로 수확전 키토산 살포는 참다래 과실의 과피장해 억제, 경 도 저하 지연을 통하여 저장성을 증진시키는데 기여하였다.

Bee pollen is rich in various nutrients and called as natural complete food. Bee pollens of acorn (Quercus acutissima) and actinidia (Actinidia arguta) are the most collected in Korea. But stiff pollen wall hinders dissolution of polysaccharides and lowers extraction efficiency. Thus, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized actinidia pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of actinidia pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in actinidia pollen germinated with A. mellea. The total polyphenol content of the lyophilized actinidia pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized actinidia pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized actinidia pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of actinidia pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from actinidia pollen inoculated with A. mellea.

제주지역 참다래 과원의 영양진단을 통해 참다래 수체 내 영양상태를 파악하여 시비기 준을 설정하기 위해 제주지역의 참다래 과원 110 곳의 토양과 참다래 잎을 조사하였다. 참다래 수체의 영양진단을 위한 엽채취 시기는 엽내 성분 함량이 가장 안정된 시기인 7 월 중순~8월초가 가장 적당하였다. 참다래 엽내 무기성분 평균함량은 질소 2.74, 인산 0.20, 칼륨 1.91, 칼슘 1.91, 마그네슘 0.51% ,철 147.0, 망간 160.1, 아연 54.0mg/kg 이었다. 엽내 무기성분 함량의 예상 임계치를 설정하여 정상 범위를 구한 결과, 질소는 2.5~3.0, 인산은 0.16~ 0.24, 칼륨은 1.80~2.44, 칼슘은 1.95~2.63, 마그네슘은 0.39~0.63%였다. 제주지역 참다래원 토양의 이화학성은 pH 5.46, 유기물 9.69%, 유효 인산 220.7 mg/kg, 치환성 칼륨 1.1, 칼슘 4.1, 마그네슘 1.48 c㏖/kg, 양이온 치환용 량 16.18 c㏖c/kg이었다.

The present study was undertaken to evaluate the antagonistic effects of water extracts of Chinese gooseberry (Actinidia chinensis P.) root on the natriuretic peptide receptor system in the rat kidney. By using in vitro receptor autoradiography, specific 125I-atrial natriuretic peptide (125I-ANP) binding sites were localized in glomerulus, inner medulla, intrarenal artery, vasa recta bundle, and renal pelvis of Sprague Dawley rat kidney. These specific bindings were competitively displaced by water extracts of Chinese gooseberry root in a dose dependent manner. Also, productions of 3',5'-cyclic guanosine monophosphate (cGMP) by activation of particulate guanylyl cyclase (GC) were stimulated by ANP in the glomerular and inner medullary membranes from rat kidney. These cGMP productions were inhibited by water extracts of Chinese gooseberry root in a dose dependent manner. The inhibitory effect of water extracts of Chinese gooseberry root on activation of GC was more potent rather than on 125I-ANP bindings to these renal structures. From affinity cross-linking study, the water extracts of Chinese gooseberry root inhibited the 125I-ANP labeling of GC-coupled natriuretic peptide receptor from renal glomerulus. Intravenous infusion of ANP elicits a potent diuresis, and urinary sodium and chloride excretions in New Zealand White rabbit. The pre-treatment of intravenous water extracts of Chinese gooseberry root infusion decreased competitively various renal effects of ANP with out changes in systemic hemodynamics. These results indicate that the water extracts of Chinese gooseberry root specifically inhibits the GC-coupled natriuretic peptide receptor subtypes (NPR-A), and suggest that the water extracts of Chinese gooseberry root may contain an antagonistic molecule regulating the biological functions of ANP system in the kidney and other organs.

본 연구의 목적은 토종다래의 용매별 추출물에 따른 약리활성에 대한 검증 및 효능 평가로서 토종다래의 항산화, 항염증에 대한 효과를 확인하였다. 염증 반응은 자극이 가해지면 histamine, serotonin, prostaglandin과 같은 혈관 활성물질에 의해 혈관 투과성이 증대되어 염증을 유발하고 cytokine, free radical, lysosomal enzyme 등 다양한 매개 인자가 관여한다. 자극에 의한 macrophage cell의 염증반응은 tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-1β(IL-1β)와 같은 pro-inflammatory cytokine의 발현이 유도되고, inducible nitric oxide synthase(i-NOS)와cyclooxygenase-2(COX-2)에 영향을 받는 유전자의 발현을 자극하게 되어 nitric oxide(NO) 등의 염증 인자가 생성된다. 이에 따라 토종다래 추출물의 항염증에 대한 연구를 위해 이에 영향을 주는 인자인 i-NOS, COX-2의 단백질 발현억제 작용을 확인하였다. 그 결과, HKE > HKA > HKW 순서로 높은 효능을 확인 할 수 있었다. 가장 효과가 좋은 HKE 처리군에서 다양한 염증성 인자의 mRNA 발현량을 확인하였다. 측정 결과, HKE(2,000 μg/mL)는 i–NOS, COX-2, IL-1β, IL-6, TNF-α mRNA 발현이 각각 93.2%, 27.9%, 96.4%, 89.4%, 73.9% 억제되는 효과를 확인할 수 있었다. 또한, HKE의 nuclear factor kappa B(NF-κB) 단백질 발현에 농도의존적으로 유의미한 결과를 확인하였으며, 이에 토종다래의 항염증효과는 LPS에 의한 TLR4의 자극에서 NF-κB 경로의 완화로 나타는 것임을 검증하였다. 결론적으로 토종다래는 70% ethanol 추출물(HKE)의 항염증 효과가 가장 높았으며, HKE는 대식세포에서 NF-κB 염증관련 경로의 억제로 세포 내 mRNA 및 단백질 수준에서의 염증인자들의 생성을 저해하여 항염증 효과가 명백히 확인되었다. 향후 본 연구팀은 토종다래의 항염증과 관련된 유효성분의 분리정제 및 구조분석을 진행할 예정이다.

The purpose of this study was to evaluate the effect of superheated steam drying on physicochemical and microbial characteristics of Korean traditional actinidia (Actinidia arguta) leaves. Actinidia leaves were dried at steam temperature of 350℃ and oven temperature of 150℃ for 40-200 sec. Moisture content and water activity decreased with increasing the drying time, while color values including L, a, and b values and total color difference (ΔE) increased as drying time increased. The relationship between moisture content and water activity showed an exponential fit with high correlation vlaue (R2=0.9909). Total phenolics and flavonoids content and antioxidant activity such as DPPH radical scavenging activity, ABTS radical scavenging activity, and FRAP assay of dried actinidia leaves increased with increasing the drying time up to 160 sec, but dramatically decreased at drying of 200 sec. The numbers of total areobic bacteria of leaves was not detected at drying time over 120 sec and coliform of all the samples was not detected. As a results, the superheated steam was an very effective drying method of increase to the nutritional and sanitary quality of dried Korean traditional actinidia leaves.

Background : Alzheimer`s disease (AD) is characterized by neuronal loss and extracellular senile plaque, whose major constituent is β-amyloid (Aβ), a 39-43 amino acid peptide derived from amyloid precursor protein. In cultures, Aβ directly induce neuronal cell death and can include excessive generation of free radicals and peroxidative injury to proteins, lipids, and other macromolecules. Actinidia arguta, generally called hardy kiwifruit, has been reported to possess anti-inflammatory, anti-allergic and antioxidative properties. The present study aims to investigate the neuroprotective effect of the leaves and stems of A. arguta using in vitro cultured neurons and in vivo experimental animals. Methods and Results : Primary cortical neuronal cultures were prepared using Sprague-Dawley (SD) rat fetuses on embryonic days 15. Neurotoxicity experiments were performed on neurons after 3-4 days in culture. Cultured neurons were treated with 10 μM Aβ (25-35) for 24 h to produce neurotoxicity. In addition, cultured neurons were treated with H2O2 (100 μM) for 15 min and then incubated for 12 h in H2O2-free medium. Viability of cultured neurons was measured by a colorimetric MTT assay. Hoechst 33342 staining of neurons was carried out to examine Aβ (25-35)-induced apoptotic neuronal death. A. arguta over the concentration of 10 to 50 ㎍/㎖ prevented Aβ (25-35) (10 μM)-induced apoptotic neuronal death, and inhibited H2O2-induced decrease of MTT reduction rate. These results suggest that oxidative stress is implicated in Aβ (25-35)-induced neuronal apoptotic death. Memory impairment was produced by intracerebroventricular (i.c.v) microinjection of 15 nmol Aß (25-35) and examined using passive avoidance test in ICR mice. Chronic treatments with A. arguta (14 days, p.o.) protected memory impairment induced by Aß (25-35). Conclusion : The present study suggests that A. arguta has a therapeutic role for preventing the progression of neurodegenerative disease such as AD.

상온(20~25℃)에서 후숙(0일~30일)된 참다래의 품질 및 영양성분 함량분석을 수행하였고 최적 후숙 기간을 구명하 기 위해 항산화활성, 단백질 분해능과 관능평가를 수행하 였다. 기간별 후숙된 참다래의 품질은 경도와 산도가 20일 이후부터 급격히 감소되는 경향을 보였고 식이섬유, 특히 수용성 식이섬유는 20일 이후에 함량이 크게 증가 하였으 며 유리당과 유기산 함량도 대체적으로 20일 이후에 뛰게 각 증가와 감소하는 경향을 보였다. 총페놀과 총플라보노 이드 함량은 20일 이후에 함량이 유의하게 높았고 아질산 염 소거능도 이와 유사하게 증가하는 경향을 보였다. 또한 DPPH radical 소거능을 통한 항산화 효과와 tyrosinase 억제 능을 이용한 미백효과는 기간이 길어질수록 감소하였는데 이는 비타민 C 함량 패턴과 유사하였다. 참다래에 함유된 단백질 분해효소 actinidin을 SDS-PAGE 분석을 통해 확인 하였고 함량과 이의 효능을 조사한 결과 후숙 기간이 경과 됨에 따라 actinidin의 함량 과 단백질 분해능도 점차 증가하 였으며 15~20일 이후에 급격히 높아지는 것을 확인 하였다. 후숙 기간별 참다래의 관능평가도 수행 하였는데, 20일 째 가 선호도가 가장 높았으며 흥미롭게도 30일 째에는 오히 려 선호도가 떨어지는 경향을 보였다. 현재까지 저온 저장 된 참다래의 상온 보관시 최적 후숙 기간에 관한 연구는 미흡한 실정으로 본 연구 결과는 소비자에게 저장 보관된 참다래의소비및 섭취에있어서도움이 될것으로생각된다.