Few studies have reported how temperature influences growth and development of succulents, including anthocyanin production, which could fetch better prices in the market, and understanding the factors influencing such pigments would benefit farmers. The present study investigated the effect of temperature (10°C, 20°C, and 30°C) on the growth, development, and anthocyanin concentrations in Echeveria agavoides and E. marcus. In E. agavoides, similar growth performance was observed at 10°C and 20°C based on plant height and diameter. However, subjecting the species to a high temperature of 30°C resulted in a decrease in plant height. In E. marcus, optimal growth performance was observed at 20°C. Different temperatures did not significantly affect succulent quality and color hues. Only L* values were significantly different among the Hunter’s Lab values. Similar results were observed following anthocyanin and image analyses, both of which were not significantly affected by temperature. However, an intense red pigment was observed at 20°C compared with the green pigment observed at 10°C and 30°C based on the image analysis. The results suggest that temperature influences growth, development, and anthocyanin content of Echeveria succulents, and 20°C could be the optimal temperature for the cultivation of the species.

Shading is a key element in the control of light intensity and is usually used during the summer when sunlight is at its most intense. Succulents are ornamental crops that have beextremely popular because of their leaf structure and other characteristics. A study was conducted to determine the effects of shading on the growth and development as well as the anthocyanin content of two Echeveria species: Echeveria agavoides and E. marcus. Three levels of shading were used, namely no shading/full light treatment, partially shaded conditions, and well-shaded conditions, which were achieved by placement inside controlled growing chambers for 4 weeks. The results revealed the bolting of growth in both height and diameter for both species when grown under shaded and partially shaded conditions, which were abnormal compared withthe typical development. CIELAB color results also showed thata* was significantly affected by shading levels, exhibiting a higher positive value for succulents under full light or no shading. These results were consistent with the anthocyanin analysis, where the highest contents were identifiedinthe species grown under full light. The image analysis also confirmed a higher percentage area based on the smart segmentation for red pigments compared withthat for green pigments.

This study was conducted to determine the effects of light intensity on the growth and development as well as the anthocyanin content of two Echeveria species, namely Echeveria agavoides and E. marcus. Three light intensity levels (high, 150 μmol･m-2s-1; intermediate, 75 μmol･m-2s-1; and low, 35 μmol･m-2s-1) served as the treatments, which were replicated four times. The results revealed that the tallest and largest plants were those under low light conditions. It was observed that there was a decline as the light intensity increased, which is attributed to the coping mechanisms of plants to search for light sources, which has a similar effect to bolting or an increase in the node-to-node distance. CIELAB color values of L* and a* for both species were significantly affected by the light intensity, indicating changes in the lightness of hue and green-to-red color pigmentation in plants. These results were strongly reflected in those of the anthocyanin content analysis, where a direct increase in the concentration was observed with increasing light intensity. The results of the anthocyanin analysis were also supported by the histogram, smart segmentation images, as well as the ratio of red and green pigments found in the images. Thus, a high light intensity should be used to increase the quality and provide conducive growing conditions for both succulent species.

LC-DAD-ESI/MS를 이용하여 국내 자색벼 품종에 대해 개별 안토시아닌 조성 및 함량을 평가한 결과는 다음과 같다. 1. 자색벼 품종에서 분리된 모든 개별 안토시아닌의 화학구조는 MS fragment 패턴을 확인하여 cyanidin을 base로 한 unknown 화합물 1종을 포함, cyanidin 3,5-diglucoside, cyanidin 3-glucoside, peonidin 3-glucoside의 총 4가지 개별성분이 분리 및 동정되었다. 2. Cyanidin 3-glucoside 및 peonidin 3-glucoside이 주요성분으로 cyanidin 3-glucoside의 경우 90% 이상의 가장 높은 함량 비중을 나타냈으며, 개별성분별 평균 함량은 cyanidin 3-glucoside > peonidin 3-glucoside > cyanidin 3,5-diglucoside > unknown(cyanidin based)의 순으로 나타났다. 3. 흑진주벼의 총 안토시아닌 함량은 408 mg/100 g으로 흑남벼보다 약 6배 정도 높은 함량을 나타내었다.

The purpose of this study was to obtain basic data for using Aronia as a functional food material. The composition of anthocyanin was characterized and quantitated by LC-MS/MS, HPLC, and UV-VIS spectrophotometer techniques, respectively. The anthocyanin content was analyzed by temperature, time, pH, and the addition of citric acid. The UV-VIS spectrophotometer used for analysis of anthocyanin is less accurate than the LC-MS/MS method used in recent years. In the past, cyanidin-3-Glucoside was reported to be a major anthocyanin that contains Aronia. However, LC-MS/MS analysis in this study confirmed cyanidin-3-galactoside to be the major compound. The anthocyanin content of the Aronia powder began to decrease sharply at a temperature of 65 ℃ or higher when heated for 24 hours. In an aqueous solution of Aronia, the anthocyanin content was reduced by 50% at 65 ℃ for 10 hours and decreased by 85% at 85 ℃ within 10 hours. Above pH 8, the anthocyanin content was reduced by more than 50%. The results of this study will provide useful information to maintain anthocyanin content in the manufacturing process of Aronia. It could also be used to ensure the stability of anthocyanins in similar species of berries.

In sustainable viticulture, green manure represents a safe and non-polluting way to bring large quantities of organic matter into the soil. The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. This study was conducted to evaluate the effects of green manure fertilization on yield and quality of the Campbell Early grape variety in the organic vineyard. Hairy vetch was the more productive green manure crop, yielding higher dry materials. Average yields of grape were significantly greater in hairy vetch + rye (13.02 ton ha-1) than nature weed (11.65 ton ha-1), respectively. The concentrations of total as well as individual anthocyanins were consistently higher with hairy vetch treatment compared with rye and nature weed, thus making the green manure cultivation is an environmentally friendly cultivation to increase the yield and anthocyanin contents in organic grape.

This study was carried out to determine environmental factors affecting the anthocyanin content of colorfleshed potato (Solanum tuberosum L.) tubers. After planting of two color-fleshed potato cultivars of ‘Hongyoung’ and ‘Jayoung’ in different 14 locations, their soil chemical properties and meteorological data were evaluated, and anthocyanin contents of tubers were analyzed after harvest, additionally their relationship among them was analyzed through correlation analysis. In comparison with two cultivars, purple-fleshed ‘Jayoung’ potatoes showed higher anthocyanin content than red-fleshed ‘Hongyoung’ in almost locations. When locations were divided to three categories (highland, sub-highland and lowland) according to altitude, in general, highland-grown tubers had the higher content of anthocyanin compared to those grown in lowland. An analysis of the results of chemical components of soil showed that anthocyanin content of color-fleshed potato tubers was negatively correlated with the pH of soil. In addition, mean temperature and minimum temperature from 80 to 100 days after planting most significantly affected on the accumulation of anthocyanin in color-fleshed potato tubers, that is, higher content of anthocyanin was observed in tubers produced in locations with lower mean temperature and minimum temperature from 80 to 100 days after planting. This information can be useful to producers and industries in selection of proper fields for the production of color-fleshed potato tubers having high quality in Korea.

신품종 ‘보석찰’쌀보리는 안토시아닌 함량이 높은 자색 찰성 쌀보리 신품종으로 국립식량과학원에서 도복과 내한성이 강하고 찰성으로 립백도와 정맥율이 높으며 취반특성이 우수한 특성을 지닌 ‘찰쌀보리’와 ‘함안군북찰쌀보리’를 인공교배한 후 계통육종법으로 육성하였다. 전작 및 답리작 지역적응시험을 실시한 결과 답리작 적응성이 높으며, 도복 등 내재해성에 강한 특성과 안토시아닌 함량이 높은 자색영을 가진 계통임이 입증되었다. 출수기는 전작에서 새찰쌀보리보다 1일 빨랐으며, 답리작은 새찰쌀보리와 같았고, 성숙기는 전작과 답리작에서 새찰쌀보리와 같았다. 간장은 87 cm, 수장 5.0 cm, m2당 수수는 601개로 새찰쌀보리보다 많았고, 천립중은 29.0 g으로 소립종이었다. 단백질 함량은 표준품종인 새찰쌀보리와 비슷하였다. 안토시아닌 함량은 180.1 ㎍/g이었으며, β-glucan과 아밀로스는 새찰쌀보리와 비슷하였으며, 흡수율과 퍼짐성은 다소 높았다. 수량성은 전작에서 3.44 MT/ha, 답리작에서 3.56 MT/ha이었다.

딸기 유전자원 중 원예적 특성이 우수하여 육종 소재로 활 용 가능성이 높은 19품종을 대상으로 완숙된 과실을 부위별 (과피 및 과실 전체)로 안토시아닌 함량을 분석하고 색차계를 이용하여 비파괴적인 방법으로 안토시아닌 고함유 계통을 선 발할 수 있는지에 대하여 검토하였다. 그 결과 딸기의 과피는 22.04～87.16 mg · 100 g -1 FW, 과실 전체는 1.98～30.16 mg · 100 g -1 FW의 범위에 분포하여 측정 부위와 유전자원에 따라 안토시아닌 함량의 변이가 큼을 알 수 있었다. 과실 전 체를 기준으로 하였을 때, 조사된 유전자원 중 ‘Sachinoka’, ‘Tamar’, ‘Amaou’, ‘Summerberry’ 순으로 안토시아닌 함량 이 높게 나타났다. 과피와 과실 전체 부위의 안토시아닌 함량 간의 상관계수가 0.867로 높아 과피를 기준으로 하여 과실 전 체의 안토시아닌 함량을 추정할 수 있을 것으로 생각된다. 또 한 과실 전체의 안토시아닌 함량과 측정된 색차계의 L*값 (-0.791) 및 b*값(-0.772)에서 높은 역의 상관관계를 보였다. 따라서 향후 딸기 육종 프로그램에서 안토시아닌 고함유 계 통을 신속하게 선발할 때, 색차계를 보조적인 방법으로 활용 할 수 있을 것으로 판단된다.

Dandelion has been widely utilized for medicinal and edible purposes. This research was conducted to evaluate the effect of supplemental LED (light-emitting diode) light on germination, growth characteristics and anthocyanin content of dandelion (Taraxacum officinale) seedling using LED blue (460 nm), red (660 nm, R), blue + red (B:R=6:4) and fluorescent lamp light treatment. By LED illumination to T. officinale seed germination speed was delayed, and germination rate was the highest in the fluorescent light. The growth characteristics (plant height, number of leaves, root length and fresh weight) were greatly influenced by supplemental LED light compared with control treatment, and the growth promotion was the most effective in the red LED illumination. After 60 days of red and mixed LED light treatments, anthocyanin content of dandelion plants was significantly changed. The anthocyanin content was increased by 12~19 mg/100 g under the red LED and the mixed light conditions compared with the control and the blue LED. Results indicate that illumination with red and mix LEDs, compared with other light treatments, is beneficial for promotion of growth and anthocyanin content in dandelion.

A New Six-rowed Naked Barley Cultivar “Ganghocheong” with High anthocyanin content and green aleurone the cross between Iri26 and Sagukgwa67 by the National Institute of Crop Science, RDA in 2009. An elite line HB14190-B-61-3-2-3-3-5-1 was selected in 2004 and designated as ‘Iksan 92’. It showed good agronomic performance in the regional yield trials (RYT) from 2007 to 2009 and was released with the name of “Ganghocheong” possessing high anthocyanin content and green lemma non-waxy endosperm. The average heading and maturing dates of “Ganghocheong” were April 20 and May 29 in paddy field, which were two days earlier and same than those of the check cultivar ‘Saessalbori’ respectively. The new cultivar, “Ganghocheong”, had 81cm of culm length and 6.0cm of spike length. It showed 702 spikes per m2, 60 grains per spike, 30.0 g of 1,000-grain weight, and 794 g of test weight. “Ganghocheong” showed better resistance to BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saessalbori’. Its average yield of the pearled grain in the regional yield trial was 4.02 MT/ha in paddy field, which were 1% lower than that of the check cultivar, respectively. Anthocyanin content was 122.1㎍/g that was higher 2 times than that of zasoojeongchalssalbori. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.

이 연구는 기능성 사료용 보리의 생산 기술을 개발하기 위한 기초자료를 얻고자 식물체 전체에 안토시아닌이 함유된 겉보리인 보안찰보리 품종을 이용하여 추비 및 수확시기별 생육과 사료가치 및 안토시아닌 함량의 변화를 검토한바 그 결과는 다음과 같다. 1. 초장은 추비가 빠를수록 유의하게 컸고, 수확시기에 따른 유의성이 없었으며, 건물률은 추비시기가 빠를수록, 수확시기가 늦을수록 높았다. 2. 단백질 함량은 추비와 수확시기가 늦을수록 높았으나, NDF, ADF 및 TDN 함량은 추비시기와 수확시기에 따른 유의성이 없었다. 3. 총 안토시아닌 함량은 추비시기가 빠를수록 증가하였으며, 조성별로는 cyanidin-3-g1ucoside(C3G)와 delphinidin 에서 유의성이 있었다. 4. C3G와 delphinidin은 출수 후 25일, pelargonidin-3-glucoside (P3G), cyanidin 및 pelargonidin은 출수 후 30 일부터 검출되었으며, 수확시기에 늦어짐에 따라 유의하게 증가하였다.

Recently, barley is gaining renewed interest for food due to nutritional and functional characteristics. and many breeder developed new barley varieties containing nutritional and functional components. Anthocyanin have been well characterized for its bioactive properties, anti-oxidant, anti-cancer, anti-proliferative and anti-inflammatory properties. Some colored varieties with purple lemma that is a kind of anthocyanin were developed. We have to find out the harvesting time containing maximum anthocyanin content. In this study, we analyzed anthocyanin content at intervals of 4-6days after heading date. We analyzed Delphinidin-3-glucoside(Del3g), Cyanidin-3-glucoside (Cya3g), Pelargonidin-3-glucoside(Pel3g), Peonidin(Peo3g), Malvidin-3-glucoside(Mal3g) using the HPLC with Xterra C18 column and UV Detector. Anthocyanin was extracted with 80% MeOH solution containing 1% HCl for 24hr. Mobil phase composition of HPLC was 45% Acetonitrile solution with 10% Formic acid. Anthocyanin content was increased rapidly from 30day after heading date to 35day. At 35-37day, anthocyanin content was maximum. Thereafter, it was decreased rapidly to 40day.

We investigated outer appearance of brown rice(BR) and its anthocyanin content of Josangheugchal a colored rice variety as affected by different transplanting date(from May 15 to June 24, 10 day interval, 5 times). Thickness of BR was mainly 1.6～2.0mm which occupied 96.7% ~ 99.5% under different transplanting date. As transplanting time was late, mode of thickness of BR was clearly decreased : 2.0mm on May 15 and May 25, 1.9mm on June 4 and June 14, and 1.8mm on June 24. There was statistically significant difference in length, width and 1000 grain weight of BR according to both transplanting date and thickness of BR. There was highly significant positive corelation between thickness and length of BR and between thickness and 1000 grain weight of BR. Anthocyanin content linearly increased from 119.7mg/100g on May 15 to 384.4mg/100g on June 24 as transplanting time was late, and was highest in 1.8~1.9mm thickness of BR. Coefficient of variation for anthocyanin content by thickness of BR was lowest on June 24. According to the facts stated above it is recommendable to transplant Josangheugchal in late June to produce highly marketable colored rice.