정서적 양극화(affective polarization)에 관한 그동안의 연구들은 그 이론적 시각의 차이에도 불구하고 정서적 양극화가 당파적 정렬(partisan sorting)과 관련되어 있을 가능성을 시사한다. 이에 이 연구는 한국 유권 자들의 정서적 양극화와 이념적 정렬(ideological sorting)의 관계를 18 대, 19대, 20대 대통령선거 여론조사 결과를 이용하여 살펴보았다. 분석 결과, 우선 한국 유권자들의 정서적 양극화와 이념적 정렬은 동시에 진 행되었다고 할 수 있다. 즉, 18대 대선 이후 양당 지지자들이 점차 진보 와 보수로 결집하면서 동시에 이들 사이의 정서적 양극화가 심화한 것이 다. 또한, 이러한 이념적 차이의 확대와 정서적 양극화는 실제로 밀접한 연관이 있었던 것으로 나타났다. 핵심적으로, 지난 세 차례 대선을 거치 면서 이념 성향이 정서적 양극화에 미친 영향은 계속해서 강해졌다. 이 것은 이념적 정렬에 따른 양당 지지자들 사이의 이념적 차이의 확대가 정서적 양극화에 영향을 미쳤다는 것을 의미한다. 종합적으로, 이 연구는 적어도 18대 대선 이후 한국 유권자들의 정서적 양극화가 이념적 정렬과 밀접한 연관이 있었다는 것을 시사한다. 정서적 양극화의 원인 중 하나 가 이념적 차이의 확대일 수 있는 것이다.

The purpose of this research is to better understand what factors influence on households’ waste sorting behavior at source in Mongolia. To accomplish the goal, this study conducted online survey with self-report questionnaire method and the 353 participants’ data were collected through Facebook groups and pages related to house owners’ associations and waste recycling related non-profit organizations. The results show that the underlying factors determining the intention of waste sorting at source are (1) motivational bases such as prior beliefs and social norms; (2) and an ability/knowledge about the waste sorting at source. Furthermore, the intention is crucial predictor of the real behavior of waste sorting at source, but the most important one is an opportunity to do so. The conclusions and the recommendations are provided.

통합항해시스템(INS)은 기존 항해 장비들을 통합하여 부가가치를 제공하는 장치로써 항해 업무 수행을 위한 정보와 기능을 다기능표시장치에(MFD)에 통합하는 것으로 정의된다. IMO 성능 기준은 각 업무에 대한 최소 요구사항을 명시하고 있지만, 장비 및 기 능의 목록은 정의하지 않아 제조사마다 INS의 구성이 상이하고 사용자 관점에 기반 한 지침 또한 부족한 실정이다. 본 연구는 선박 운 용상황 및 수행 업무에 따라 사용자가 요구하는 정보를 분석하고, 이를 INS의 MFD에 효과적으로 구조화하여 INS의 사용성을 높이기 위해 수행되었다. INS 관련 국제 기준 및 제조사의 구성 장비 목록을 분석하여 필수 항해 정보들을 선별하고 MFD 사용 경험이 있는 선박 운항자를 대상으로 카드 소팅 테스트를 실시하여 각 INS 업무에 요구되는 정보들을 분류하도록 하였다. 연구의 결과는 제조사들 이 제품 설계 시 사용자 경험을 반영한 정보 구성에 기본적인 가이드로 활용될 수 있을 것이다.

Oral squamous cell carcinoma (OSCC), which accounts for approximately 90% of oral cancers, has a high rate of local recurrence and a poor prognosis despite improvements in treatment. Exosomes released from OSCC cells promote cell proliferation and metastasis. Although it is clear that the biogenesis of exosomes is mediated by the endosomal sorting complex required for transport (ESCRT) machinery, the gene expression pattern of ESCRT, depending on the cell type, remains elusive. The exosomal release from the human OSCC cell lines, HSC-3 and HSC-4, and their corresponding gefitinib-resistant sub-cell lines, HSC-3/GR and HSC-4/GR, was assessed by western blot and flow cytometry. The levels of ESCRT machinery proteins, including Hrs, Tsg101, and Alix, and whole-cell ubiquitination were evaluated by western blot. We observed that the basal level of exosomal release was higher in HSC-3/GR and HSC-4/GR cells than in HSC-3 and HSC-4 cells, respectively. Long-term gefitinib exposure of each cell line and its corresponding gefitinib-resistant sub-cell line differentially induced the expression of the ESCRT machinery. Furthermore, whole-cell ubiquitination and autophagic flux were shown to be increased in gefitinib-treated HSC-3 and HSC-4 cells. Our data indicate that the expression patterns of the ESCRT machinery genes are differentially regulated by the characteristics of cells, such as intracellular energy metabolism. Therefore, the expression patterns of the ESCRT machinery should be considered as a key factor to improve the treatment strategy for OSCC.

In the anchovy boat seine fishing boat, it is necessary to select other aquatic organisms other than live anchovies, which are the target species of catch. By making a rotating roller sorter using hydraulic pressure, the anchovy sorting amount was compared and the sorting accuracy of the rotary roller sorter, and the discharge speed of butter fish and jerry fish according to the number of roller revolutions were analyzed. The rotating roller sorter increases the weight of the sorted raw anchovy by 54%, 74% and 91.5% compared to the round bar fixed type, so it can reduce the required time by an average of 73.2%. As a result of converting the sorting accuracy to the weight of pure anchovies excluding the catch weight, the round bar fixed type was 89%; however, the average of the rotating roller sorter was 97.7%. Thus, the sorting accuracy of the rotary roller sorter was further improved by about 8.7%. The roller speed moved 7% at 300 rpm, 7.5% at 600 rpm, and 16% at 900 rpm, so butter fish were discharged overboard 10% faster than jelly fish on average. In addition, the average feed speed of butter fish and jelly fish is 1,400 mm/s when the roller rotation speed is 300 rpm, 1,480 mm/s at 600 rpm, and 1,850 mm/s at 900 rpm. A Φ58 mm roller rotates once it moved about 1.23 mm. In the future, a follow-up study of quantitative evaluation is needed targeting more non-target fish species of anchovy boat seine.

Consumer demand for imported and craft beer is increasing. Almost all the malt used by craft beer companies is imported. This study sought to develop a malting technology to expand the use of domestic barley, the main raw material for beer, and to examine its influence on the quality of domestic malt. The Hopum cultivar of beer barley was purchased and used at the Jeollanam-do Seed Management Center. A pilot-scale malting facility built by the National Institute of Crop Science was used. One sack (40 kg) of barley was sieved through screening sieves, and the barley was classified according to size (3.1, 2.8, 2.5, 2.2, and 2.2 mm), with the size-related composition being 18.1, 48.9, 23.3, 6.4, and 1.0%, respectively. The malt yields were 89.0, 87.6, 86.6, 82.2, and 76.1%, respectively. The coleoptiles elongation decreased as the barley size decreased. The protein content of malt tended to be high at small particle sizes of 2.2 mm. The soluble solid content of wort prepared from malt revealed good values of 17.4-17.5oBx, except for 15.3oBx for particles <2.2 mm in size. According to barley size in the malting process, these findings should be valuable quality indicators of malt.

복숭아는 대표적인 여름 과일이자 알레르기를 유발하는 식품으로 밀폐되고 집약적인 생산환경으로 인해 시설 내 유기분 진, 농약, 복숭아털이 발생하기 때문에, 열악한 작업환경으로 인한 작업자들이 어려움을 많이 겪고 있다. 본 연구에서는 복 숭아 선별작업장에서 발생하는 미세먼지를 위치별, 작업별, 입경별로 모니터링함으로써 저감 및 대책 마련을 위한 기초자 료를 확보하고자 하였다. 미세먼지 모니터링 결과 지역별로 는 선별과정에서 미세먼지의 발생이 높은 것으로 나타났으며, 주로 기계적인 과정을 통하여 발생하는 10μm 이상의 미세먼 지로 나타났다. 본 연구를 바탕으로 향후 작업환경의 개선을 위해서 공정 중 미세먼지가 주로 발생하는 지역 및 미세먼지 의 물리적 특성을 고려하여 저감시설 및 개인보호구의 착용이 요구된다.

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 106 cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 106 cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

본 연구에서는 고추의 수확 및 유통 단계에서 미생물 오염 수준과 그 발생원인을 확인하였고, 이를 바탕으로 수확 단계에 사용된 작업자 장갑의 교체와 온도 관리 및 소형 플라즈마 발생장치를 사용하여 신선 고추에 대한 미생물 오염에 저감화 방안을 모색하고자 하였다. 작업자 장갑 미교체 시 3시간 기준으로 작업 전과 후의 일반세균 미생물 오염증가율이 약 190% 증가하는 것으로 확인되었고, 이에 반해 교체한 작업자의 장갑의 미생물 오염증가율은 15% 증가하는 것으로 나타났다. 유통과정 중 보관 온도는 냉장 보관 보다 상온 보관일 때 미생물 오염 수준이 더 높은 것으로 나타나 냉장 보관이 미생물 오염 예방 에 더 효과적인 것으로 나타났다. 추가로 소형 플라즈마를 이용하였을 때 초기 균 수 대비 5일차에서 일반세균 3.0 log CFU/g 감소, 대장균군, 효모/곰팡이는 불검출로 나타났고 플라즈마 발생장치 없는 경우 5일차에 대장균군이 2.1 log CFU/g 수준으로 검출되어 소형 플라즈마 발생장 치가 보관 및 유통과정 중의 미생물의 저감에 효과적일 것으로 판단되었다. 이상의 결과로 볼 때 수확단계에서 미생물학적 위해요소 발생원인 중 하나인 작업자의 장갑을 주기적으로 교체하고 현장에서 쉽게 수행 가능한 냉장 유통 방법과 신선 고추의 수확 후 보관 및 유통 중에 소형 플라즈마 장치를 이용한다면 고추의 미생물 오염을 감소 시키는데 효과적인 것을 확인할 수 있었다. 또한 고추 이외에도 다른 신선농산물의 수확, 선별 단계나 유통단계에 미생물의 오염을 예방 또는 저감화 하는데 이용이 가능할 것으로 생각된다.

This study has described as a way of establishing an automated system that allows sorting of living fish caught at sea by weight, and producing and testing as prototypes. The live fish that applied for this research is named as a croaker. 30(thirty) times of measurements with the fishes were applied for screening initially. As a results, it was the highest deviation rate of 19.7% within 2g of their initial weight. In addition, it showed the lowest deviation rate of 8.2% below 10g of them. Throughout the measuring experiments, it was found that the error that occurred during weighting was friction with the upper surface of the load cell on the circular sorting plate. Once the friction was removed, it was figured out that the average deviation in weight per each fish was measured as 0.76g. This error should be able to be eliminated by improving the material of machine elements, the surface material of the load cell. Under this circumstance, this pilot test in sorting living fishes by weight was brought adequate results. The existing sorting device was performed at 4,500 fishes per hour as a sorting speed. The sorting device developed in this research improved productivity by over 33% by setting the sorting speed in 6,000 fishes per hour.

Overstock in aquaculture is a matter of concern in aquaculture management. To sort fish based on fingerling size in case of overstocking is an important problem in aquaculture farm. This study aims to determine the amount of fry overstock and sorting time in aquaculture farm. This study builds a mathematical model that finds the value of decision variables to optimize objective function summing up the fingerling purchasing cost, aquaculture farm operating cost and feeding cost under mortality and farming period constraints. The proposed mathematical model involves following biological and economical variables and coefficients: (1) number of fingerlings, (2) sorting time, (3) fish growth rate and variation, (4) mortality, (5) price of a fry (6) feeding cost, and (7) possible sorting periods. Numerical simulation is presented herein. The objective of numerical simulation is to provide decision makers to analyse and comprehend the proposed model. When extensive biological data about growth function of fry becomes available, the proposed model can be widely applicable to real aquaculture farms.

Seafood is attracting attention as a future food industry. In recent years, the demand for fishery equipment of mechanization, automation, and unmanned was increased due to the environment affected by seafood processing, stricter regulations on safety, decline and aging of fishery worker. Ark shell (Scapharca subcrenata) was being produced in many steps in the production process. The process has been made such as collection–landing–washing–first sort (goods/non-goods)–transports– second sort (size). It was undergone first and second steps by delivering to the consumer. Here, the first step is to sort goods to collection and the second step is to sort by size. The fishery workers need ten people in first step and six people in second step. The workload of one hour per kg is 4,247 kg/h in first step and 2,213 kg/h in second step. In addition, the goods ratio by work process was 79% in first step and 98% in the second step. In this process, a lot of fishery worker and working time is needed. Therefore, this study developed elemental techniques for an automated size sorting system considering the working process problem, time and situation for washing and sorting of ark shell.

Size-sorted graphene nanoplatelets are highly desired for fundamental research and technological applications of graphene. Here we show a facile approach for fabricating size-sorted graphene oxide (GO) nanoplatelets by a simple centrifugal method using different dispersion solvents. We found that the small-sized GO nanoplatelets were more effectively separated when dispersed in water or dimethylformamide (DMF) than in an alkali aqueous solution. After several iterations of the centrifugation, the sizes of GO in the supernatant solution were mostly several micrometers. We found that the GO area was not strongly correlated with the C-O content of the GO dispersed in water. However, the size-sorted GO nanoplatelets in DMF showed different C-O content, since DMF can reduce GO nanoplatelets during exfoliation and centrifugation processes.

90년대 후반 이후 농촌관광에 대한 수요와 관심이 높아지면서 농촌관광과 연관된 연구가 다방면으로 증가하게 되었고 이 가운데 농촌관광자원의 유형을 구분하는 연구도 늘어나게 되었다. 어느 분야를 막론하고 대상 자체의 유형을 구분하는연구는 해당 주제에 대한 체계적인 이해를 돕기 위한 초석을 만드는데 크게 기여해왔다. 농촌관광자원의 유형을 구분하는연구는 꾸준히 진행되어왔지만 소비자들의 직접적인 의견이 반영되지 않은 채 전문가적 관점이나 통계적 유사성에 의거하여분류되어왔던 것이 사실이다. 이러한 배경 하에 본 연구는 실제 관광자원을 이용하는 이용자 중심의 시각에서 체험 중심의 농촌관광자원의 유형을 분류하였다. 이를 위하여 농촌어메니티자원 DB를 기초자료로 활용하여 마을별 관광자원을 수집하고카드 분류법(card sorting test)을 실시하였다. 카드 분류법의 피조사자는 농촌관광의 경험이 있는 대학생 32명을 선정하여진행되었으며 최종적으로 전문가 설문을 통하여 농촌자원유형 분류를 검증하였다. 조사 결과 상위 항목에서는 ‘체험하기’,‘지역향토음식 먹기/만들기’, ‘숙박하기’, ‘주변관광/경관감상’, ‘레져 즐기기’, ‘예술활동하기’의 6가지 유형으로 분류되었다.하위항목에서는 상위항목의 ‘체험하기’에서만 분리되는 특징을 보였으며 ‘지역자원활용체험’, ‘자연체험’, ‘전통체험’, ‘수확/채집체험’의 4가지 유형으로 분류되는 특징을 보였다. 본 연구는 농촌관광을 하는 관광자들의 만족도 제고와 관광자원에 대한이해도를 높이기 위하여 실시되었으며 본 연구 결과를 통하여 도출된 농촌관광자원 유형 분류는 농촌관광자원 및 지역자원을소개하기 위한 홍보자료 및 웹, 앱 메뉴 설정 등에 이용되는 등 향후 활용성이 높을 것으로 기대한다.

This study evaluated a method of sorting X and Y chromosomes based on size using the forward angle light scatter related refractive index (FSC) of a flow cytometer. Hanwoo bulls sperm were separated to X and Y chromosomes by the parameters of FSC or Hoechst 33342 intensity. As a result, using monitor program linked flow cytometry during sorting processing, the purities were or for the X-fraction and or for the Y-fraction in the two sperm sorting methods. There were no differences in the X and Y ratios (X and Y %) between the sperm sorting methods based on FSC or DNA content. The proportions of female and male embryos used for in vitro fertilization and development were or , and or when sperm were processed using the sex sorting method by FSC or Hoechst 33342. In conclusion, further study is needed to determine the optimum procedure and improve the nozzle to enhancing sorting accuracy or efficiency. Also, the findings of this study do not negate the possibility that the difference method of sperm sorting cannot use a UV laser beam.

This study was performed to design and develop an ergonomic equipment for strawberry sorting and packing in agricultural working environments. Potential risk of work related musculo-skeletal disorders associated with farm workers performing strawberry sor