This study was aimed to determine the effects of grow media on the mineral contents of the leaves and growth characteristics of strawberry grown under aquaponics system in a plant factory. For aquaculture, 12 fish (Cyprinus carpio) (total weight, 2.0 kg) were raised in an aquaponics tank (W 0.7 m × L 1.5 m × H 0.45 m, 472.5 L) filled with 367.5 L of water at a density of 5.44 kg·m-3 and total 34 of strawberry seedlings were transplanted in the pots filed with 200 g of orchid stone, hydroball or polyurethane sponge in the growing bed (W 0.7 m × L 1.5 m × H 0.22 m) laid out with holly acrylic sheet (140×60 mm, Ø80) on the top of the system. The pH and EC of the aquaponic solution was ranged from 7.6 to 4.9 and 0.24－0.91 dS·m-1, respectively. The concentration of NO3-N was about 28% lower than that of the hydroponic standard solution, and K, Fe and B were 10, 27 and 3.8 times lower, respectively; however, the mineral contents of strawberry leaves were in the appropriate ranges with lower contents in the leaves grown with sponge media. The organic content (OM), nitrogen (N), phosphorus (P), and potassium (K) of the sludge were 61.5, 5.72, 8.92, and 0.24%, respectively. The leaf area, leaf number, and dry and fresh weights of shoot at 81 DAT were significantly higher in the hydroball, and the average number of fruits per plant was significantly higher in both the orchid stone and hydroball. There was no significant difference in the fresh and dry weights of fruits. Integrated all the results suggest that the orchid stone and hydroball media are more effective to utilize nutrients in solid particles of aquaponic solution, compared to the polyurethane sponge.

Corporate reputation – the central antecedent of trust – bears the potential to create sustainable competitive advantage. However, far too many examples of companies’ socially irresponsible behavior over the past years led to a severe crisis of confidence. Disgraced companies suffer from the adverse effects of their misbehaviors at all levels. As a consequence, one of the top priorities for both practitioners and business scholars is the identification of opportunities to (re)build corporate reputation. Corporate Social Responsibility (CSR), a key driver of reputation perceptions, is a very promising one. However, as CSR is a multidimensional construct that comprises a wide range of activities, the selection of the “right” ones deems a major challenge. Based on a literature review, we advocate that news media data should be utilized to analyze which CSR dimensions are particularly likely to affect reputation perceptions. As journalists rely on companies’ press releases as a starting point for their business articles, companies need to carefully evaluate which CSR dimensions they emphasize in their communication strategy. Based on superior measures of reputation and CSR, this study utilizes reputation and news media coverage data on companies listed in the German DAX30 between 2005 and 2011. The panel data regression encompasses the multidimensional concept of CSR, presenting a six-dimensional CSR construct including environment, employee relations, community, product issues, corporate culture and corporate governance. Relevant moderating variables, namely firm and stakeholder characteristics, are investigated. In this context, the results show that the relevance of each of those six distinct dimensions differs for the formation of reputation judgements and varies across investigated stakeholder and company types: across all model specifications, negative media coverage addressing employee relations and community affects reputation perceptions. The general public primarily perceives negative news coverage as relevant for their reputation judgements. Opinion leaders seem to be less dependent on the media to learn about CSR dimensions, as only four out of twelve independent variables exert a significant impact on their reputation judgments. News coverage about product issues only constitutes a key role in the formation of reputation judgements of firms that are predominantly known from direct experiences. A particularly large amount of variation can be explained for reputation ratings of these companies as well as for reputation perceptions of opinion leaders.

본 연구는 배지의 종류에 따른 더덕과 황기의 발아와 육묘기의 생육을 구명하기 위해 수행되었다. 종자는 상업적 상토(토실이), 코이어, 암면, 우레탄스펀지에 파종하였다. 파종 후 밀폐형 식물생산시스템에서 22일간 발아 하였으며, 경상대학교 벤로형 유리온실에서 파종 후 35 일간 육묘 하였다. 양액은 EC 1.0dS·m-1, pH 6.5로 맞추 어 2일 간격으로 저면관수 하였다. 더덕의 최종발아율은 코이어와 암면 배지에서 각각 68.5%와 67.9%로 유의적으로 높았으며, 평균발아수 역시 코이어와 암면 배지에서 4.2립과 4.1립으로 가장 높았다. 황기의 경우 배지 종류에 따른 황기의 발아율은 유의적인 차이를 나타내지 않았다. 더덕의 초장과 엽면적은 암면 배지에서 각각 11.5cm와 11.3cm2로 유의적으로 높았으며, 최대근장과 뿌리의 생체중은 코이어 배지에서 5.8cm와 0.07g으로 가장 우수했다. 황기의 초장과 엽면적은 코이어 배지에서 14.0cm와 16.9cm2로 유의적으로 높았으며, 지하부의 생체중과 건물중은 0.34g과 0.03g으로 우레탄스펀지 배지에서 가장 높았다. 따라서 본 연구 결과에서 더덕과 황기의 발아와 육묘기의 생육을 위해 암면과 코이어 배지가 적합한 것으로 판단된다.

Despite its innovative and avant-garde reputation, the luxury industry initially began showing a very low commitment to new online marketing tools and it held a conservative approach to selling when compared to other sectors. Nowadays, the context has dramatically changed and luxury brands are approaching with an increasing interest social networks as well as the online selling. This research aims to clarify the current strategic approaches of the players in the different luxury markets towards the social commerce phenomenon, from both a theoretical and an empirical point of view. The purpose is to test a framework that can be used to classify luxury companies’ strategies regarding social media adoptions based on actual theories on social media. Four strategies related to the social media adoption by luxury brands have been identified: the Social brand ambassadors strategy class (low promotional content percentage and low social commerce score) includes those brands that use social media for entertainment and user engagement; the Social showcases strategy (high promotional content percentage and low social commerce score) includes those brands that use their social accounts as online catalogues; the Social infotainers strategy (low promotional content percentage and high social commerce score) includes those brands that scored high in social commerce, mainly because of the provision of informative content and brand–consumer interactions, but they were linked to more entertainment-oriented actions rather than product-related ones. Finally, the Social sellers strategy (high promotional content percentage and high social commerce score) includes those brands that have integrated social commerce into their online strategies and have subsequently exploited the potential of social media to drive online and offline sales. The database is built using original data from a content analysis of 100 luxury brands’ postings on five different social media platforms – namely Facebook, Twitter, YouTube, Instagram, and Pinterest. The total final sample included 12,132 Facebook posts, 21,216 tweets on Twitter, 1,105 YouTube videos, 10,138 Instagram pictures/videos, and 117,359 Pinterest pictures. The main findings are the following: luxury brands adopt at this stage the Social brand ambassadors and Social showcases approaches; brands belonging to the perfumery, cosmetics, jewelry and watches markets show a more developed attitude towards the social commerce; in other luxury markets, such as wine and spirits, brands still adopt a Social Brand Ambassador strategy, while managers should increase the promotional content in order develop the social commerce. The Fashion & Accessories brands show a positive relationship between the percentage of promotional content and social commerce score. This means that social commerce adoptions depend on the single brand’s strategic choices, ranging from low adoption to best practices. In general, social commerce is still not widespread; many luxury fashion brands, while presenting new collections during fashion weeks, focused on fashion shows, backstage events, and celebrities, rather than really promoting the new product lines with materials, availability, and purchasing indications. This social media approach is mainly focused on increasing brand awareness rather than increasing social commerce. If managers aim at increasing social commerce they should add direct call to action and link the contents to e-commerce market place. Automotive brands are concentrated in the Social showcases area; This sector encounters natural limitations in the introduction of social commerce due to the difficulty of selling products through the digital channel; many brands have, however, devised strategies to approach their users during the purchasing process prior to the actual transaction to take advantage of the increasing ROPO phenomenon. Conversely, the Perfumes & Cosmetics sector shows a highly fragmented approach to social commerce. The content analysis based on single post contents has shown that actually the contents are based on pictures of the products, or the brand, information on events, and a large and increasing presence of video posts based storytelling about the history of the product and the brand heritage; the most social commerce oriented posts are picture or video focused on the product. The commercial contents that aim at developing the see now, buy now approach are mainly based on video shows.

Sawdust bag cultivation was performed to understand the effect of different sawdust substrate (Quercus mongolica, Q. acutissima, Q. serrata, Q. aliena, and Q. variabilis) on the eight variety of shiitake (Poongnyunko, SANJO 701, Mori 436ho, Sanlim 10ho, Chilha 6ho, Yoogiro, Baekwhahyang, Charmaram). After inoculation, sawdust substrate block (2 kg) was cultivated at 22°C for 120 days. During the production period, the temperature was adjusted to 18°C and the relative humidity at 90%. The yield was calculated based on the fruit bodies from the first, second and third flush. As results, Poongnyunko yielded 455 g on the substrate, Q. serrata, 378 g on Q. aliena, and 333 g on Q. variabilis. Chilha 6ho yielded 304 g on Q. mongolica and Charmaram yielded 245 g on Q. acutissima. The average weight of mushrooms from Charmaram was 40-45 g on all five substrates and Q. variabilis was the best among them. In terms of phenotypic characteristics, Chilha 6ho was generally better than the others. Charmaram, Poongnyunko, Sanlim 10ho also had good characteristics. Mori 436ho, Yoogiro, Baekwhahyang developed for log cultivation showed lower yield compared to the varieties for sawdust bag cultivation. Keywords : Lentinula edodes (Shiitake), Querqus spp., Saw-dust bag culture, Yield

홍콩에서 시행되는 언어교육 정책에 따라 중문과에서는 보통화와 광동어 중 어 떤 언어도 자유롭게 선택하여 교육 언어로 사용할 수 있다. 본문에서는 문헌 연구 를 통해, 보통화만 사용하는 경우, 광동어만 사용하는 경우와 보통화와 광동어를 혼용하는 경우에 따라 15개의 언어교육형식을 설계하였다. 동일 소학교의 5개 소 그룹으로 나누어 각기 다른 언어 학습 교육법을 적용하여 학생들의 한자습득 능 력 발전에 대한 영향 관계를 조사하였다. 본 연구는 여러 개의 안과 다양한 자료를 수집하고, 삼각 논증의 방식으로 상호 비교 검증하였다. 질적 연구 방면으로는 교실 수업 분석과 교사와 학생 면담자료 등 다양한 개별 측정의 방법을 사용하여, 수업 언어가 학생들의 교육에 미치는 영 향을 이해할 수 있도록 하였다. 양적 연구 방법으로는 실험연구와 두 번의 설문조사를 시행하여 수업 언어가 학생들의 한자 능력 발전과 학습 흥미와 동기에 미치 는 영향을 이해하도록 하였다. 한자능력에 대한 시험은학교와 가정, 사회의 세 개의 범주로 나누어, 학생들이 특정한 시험 시간에 사용하는 심리적 어휘를 측정하였다. 이상을 통해 우리는 다 음과 같은 결론을 얻었다. 동일하지 않는 교육 언어 형식은 학생들의 한자습득 능 력의 발전 전체에 모두 직접적 영향을 미치고 있다. 오로지 동일하지 않은 교육 언어를 사용한 반에서도 반별로 학생들의 한자능력의 발전에서도 각기 다른 표현 이 보였다. 방문면담의 결과를 취합하고 새로 적용한 후 시행한 2차 측정에서 이 미 이 결과를 알고 있는 교사는 서로 다른 교육 언어로 한자교육을 진행하면서 교사의 교육 목표, 교육 전략, 교육 시간의 설정과 배분에 영향을 받고 있었고, 학 생들 또한 한자습득 능력의 발전과 어문 교육의 흥미도에 영향을 받아 이전과는 다른 현상을 보여주었다. 요컨대 본 연구를 기초로 삼아 미래 연구의 방향을 제시하는 것 뿐 아니라, 본 연구의 성과가 언어 교육의 정책 결정자와 교사들에게 참고가 되어 향후 중문과 교육의 교육 정책을 결정하는데 도움이 되고자 한다.

The aim of the present study was to compare two different serum-free media, modified synthetic oviduct fluid (mSOF) and modified potassium simplex optimization medium (mKSOM) containing 20% RD (RPMI1640 + DMEM, 1:1 v/v) (RD-mKSOM), for in vitro culture (IVC) of bovine embryos. After in vitro maturation and fertilization, the presumptive zygotes were cultured in two different serum-free conditions for 7 days and 9 days to evaluate blastocyst formation and hatching, respectively. Serum supplemented conventional CR2 medium was used as control. After 7 day of culture, there was no significant difference in cleavage and blastocyst formation rates among three groups (mSOF, 59.3 and 30.1%; RD-mKSOM, 65.0 and 41.5%; control, 51.6 and 38.0%, respectively). Hatching rate was significantly higher in control (69.0%) than other experimental groups (mSOF, 22.0%; RD-mKSOM, 39.5%) (P＜0.0001 and P＜0.001, respectively). Although both serum-free conditions showed lower hatching rates than serum-added control, in serum-free groups, RD-mKSOM showed significantly higher hatching rate than mSOF (P＜0.001). In addition, one-step using RD-mKSOM may facilitate IVC procedure than two-step culture system. In conclusion, the results indicate that one-step RD-mKSOM is more suitable defined culture system for IVC of bovine embryos than two-step mSOF.

Salmonellosis is one of the most common food-borne diseases in both humans and animals. The recovery of Salmonella from fecal and environmental samples by bacteriological assays takes several days. Polymerase chain reaction (PCR) has become an important technique for the rapid detection of Salmonella in a variety of samples, including feces. For rapid identification of Salmonella by PCR, 1 mL of enrichment culture was harvested after overnight incubation and DNA was extracted by heat lysis. To investigate the optimal conditions for rapid PCR detection of Salmonella, three different primer sets and three different enrichment media were used on a panel of Salmonella strains and a panel of non-Salmonella strains. The results showed that selenite cysteine enrichment broth and a primer set designed for the invA gene provided the most specific and rapid detection of Salmonella by PCR after the enrichment step.

본 연구는 시설하우스에서 비점오염원으로 인해 배출되는 오염된 농업배수를 인공습지에서 효과적으로 처리하기 위한 기초자료를 확보하기 위해 인공습지에서 여재종류, 조합방법 및 부하량에 따른 오염물질 정화효율을 평가하였다. 인공습지의 조합방법은 수직-수평흐름조, 수평-수직흐름조, 수평-수직-수평흐름조 및 수직-수평-수평흐름조로 구분하여 설계 및 시공하였으며, 여재는 왕사, 쇄석, 방해석 및 혼합여재로 구분하여 각 조에 충진하였다. 또한 농업배수 부하량을 150, 300, 600 L m-2 day-1으로 달리하여 오염물질의 처리효율을 조사하였다. 조합방법별 수처리효율은 HF-VF-HF 조합방법이 다른 조합방법에 비해 높은 처리효율을 보였으며, 최적여재는 COD 처리효율은 왕사가 가장 높았고, T-N은 쇄석, T-P는 방해석이 가장 높았다. 하지만 모든 처리효율과 경제성 부분을 고려하였을 때 최적여재는 혼합여재가 가장 효과적일 것으로 판단된다. 농업배수의 부하량에 따른 처리효율 결과 COD는 농업배수 부하량 600 L m-2 day-1까지 안정적인 처리효율을 보였으나, T-N 및 T-P는 150 L m-2 day-1≒300 L m-2 day-1¤600 L m-2 day-1의 순으로 농업배수의 부하량이 증가함에 따라 약간 감소하는 경향이었다. 따라서 시설하우스 농업배수 처리를 위한 인공습지 농업배수처리장의 최적조건은 HF-VH-HF 조합형 인공습지에 왕사, 쇄석 및 방해석이 혼합된 혼합여재로 충진하고 부하량 300 L m-2 day-1 이하의 농업배수를 주입하는 것이 최적일 것으로 판단된다.

Beauveria bassiana is one of universal insect pathogenic fungi that have been used for biocontrol agent against insect pests. This fungus has also been studied for medicinal use. To meet for commercial use, the artificial production of the fruit body of this fungus has been established by the Mushtech Co in Korea. This study was carried out to define the morphological features of the fruit body of B. bassiana developed through artificial cultivation. For the observation of mycelia growth, B. bassiana was cultured on the Sabouraud Dextrose agar plus Yeast Extract(SDAY), nut-supplemented medium, and Fe ion-supplemented SDAY at 25℃ for 15 days. The variation of colony color was observed between the different media. Strong pigmentation was observed on Fe ion-supplemented SDAY. To investigate morphological characteristics of fruit body, geminating ascospores and vegetative hyphae were observed though light microscopy and scanning microscope. During seven weeks of cultivation period, the development process of apical fertile part of stromata can be separated by the development stage of perithecia. To understand the developing process of fruit body at the transcript level, investigating process of distinct gene expression according to cultural condition and developmental stage was discussed.

Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.

바위떡풀의 기내 배양시 MS배지의 농도별로 12주간 배양한 결과 1/2 MS 배지에서 신초길이 1.9 cm, 엽수 24.7매, 뿌리수 8.0개, 신초수 절편당 11.0개로 1 MS나 2 MS 배지에 비해 효과적이었다. 1/2 MS배지를 기본으 로 하여 생장조절제를 첨가하여 8주간 배양한 결과 CPPU 농도처리시 신초길이와 뿌리수는 무처리구에서 많았으나 신초수에 있어서는 농도가 높아질수록 많아져 2.00 mg·L−1농도 처리구에서 8.2개로 가장 많아 증식에 유리한 것으로 나타났으나 TDZ, zeatin과 BA처리는 무처리에 비해 처리효과가 없는 것으로 나타났다. 그러므 로 바위떡풀을 조직배양 방법을 이용하여 대량증식 시키 고자 할 때에는 생장점을 초대배양(MS배지)한 후 신초를 CPPU 2.00mg·L−1을 첨가한 1/2 MS배지에 치상하여 다 신초를 발생시킨 후 다시 신초를 하나씩 분리하여 1/2 MS 배지에 계대하여 배양한 후 순화하면 다량의 식물을 획득 할 수 있을 것으로 생각된다.

본 실험은 돼지체외수정란을 생산하는데 적합한 배양액과 산소조건을 구명하기 위하여 NCSU(North Carolina State University)-23, PZM(Porcine Zygotes Medium)-3, PZM-4 및 TCM(Tissue Culture Medium)-199 배양액과 5% 산소조건(39, 5% O₂, 5％ CO₂, 90% N₂ 및 포화습도) 및 20% 산소조건(39, 5% CO₂ 및 포화습도)에서 돼지 미성숙난포란의 체외성숙과 체외배발달을 실시하였다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 1) 돼지 난포란을 5 및 20% 산소조건하에서 NCSU-23, PZM-3, PZM-4 및 TCM-199 배양액에 44시간 동안 성숙을 유도한 결과 난핵포붕괴율과 핵성숙률에 있어서 산소조건 및 배양액간 유의적(p>0.05)차이는 없었다. 2) 체외수정을 실시한 결과 정자 침투율, 자ㆍ웅전핵형성률, 다정자 침입률 및 평균정자수에 있어서 산소조건 및 배양액간에 유의적인 차이가 없었다. 3) 체외수정을 실시한 후, 5 및 20% 산소조건의 NCSU-23, PZM-3, PZM-4 및 TCM-199 배양액에서 배양하여 배발달에 미치는 영향을 조사한 결과, 난할률에 있어서는 유의적(p＜0.05)인 차이가 없었으며. 배발달 7일째 배반포기 발달률에 있어서 5% 산소조건에서는 PZM-3 배양액이, 20% 산소조건에서는 NCSU-23 배양액이 유의적(p＜0.05)으로 높은 결과를 나타냈다. 4) 배발달 7일째 배반포기 배의 세포수를 조사한 결과, 내부세포괴세포수와 총세포수는 산소조건과 배양액간에 통계적인 유의성이 인정되지는 않았으나, 5% 산소조건에서 PZM-3 배양액(36.8±6.5개)이 다소 높은 총세포수를 나타냈다. 위의 결과를 종합하면 돼지체외수정란의 생산에는 5% 산소조건에서 PZM-3 배양액에서 배발달을 유도하는 것이 배반포기 발달률과 세포수에 있어 효과적이라고 판단된다.

본 연구는 미성숙 돼지난자가 체외성숙배양액인 TCM-199과 NCSU-23배지에서의 배양상태를 비교하였고, 체외성숙용 TCM-199배지에 Earle'ssalt와 Hank's salt의 첨가에 의한 세포분화와 배발달 상태를 각각 조사하였다. 1. TCM-199와 NCSU-23 배양액에 각각의 supplement를 첨가하여 5% 조건하에서 체외성숙을 유기한 결과, TCM-199 배양액에서 유의성(p<0.05)이 검증되었다. 2. 체외성숙용 TCM-199배지

본 연구는 돼지 난포란의 체외성숙에 적합한 배양액을 조사하고, 아울러 산소농도가 돼지 난포란을 이용한 체외수정란의 생산에 미치는 영향을 구명하고자 실시하였다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 1. NCSU-23과 TCM-199 배양액에 10% PFF를 첨가하여 5% 및 20% 산소조건하에서 체외성숙을 유기한 결과, 배양액 및 산소조건에 따른 난핵붕괴률 및 핵성숙률에는 유의적 (P>0.05)인 차이가 없었다. 2. NCSU-23이 TCM