This study aimed to provide information useful for product development and strategies for the commercialization of weak flower tea through literature research and empirical analysis. The effect of the selection attributes for weak flower tea on loyalty according to quality was analyzed. The subjects surveyed included those in their 20s to 50s who preferred weak flower tea. The survey was conducted from January 5, 2024, to February 20, 2024. The results revealed that not only those in their 40s and 50s, but younger people also prefer weak flower tea these days. In the future, it is hoped that in addition to modern tea and coffee, research on flower tea will continue.

대규모 하천의 수량(river storage) 변동으로 인해 발생하는 지각 변형을 정량적으로 평가하기 위해, GNSS (Global Navigation Satellite System) 기반의 지각 변위 자료와 GRACE (Gravity Recovery and Climate Experiment) 인공위성 중력 자료, 그리고 WaterGAP 수리 모형 산출 자료를 종합적으로 분석하였다. 우리는 아마존 강 유역에 대해 수로에 집중되어 분포하는 하천 수량 변동을 선 형태의 하중으로 모형화하고, 이로부터 유발되는 지각의 탄성 변형을 계산해 GNSS 관측치와 비교하였다. 이를 통해, 이 지역에서 발생하는 계절적 지각 변위 중 하천 수량 변동에 기인하 는 성분을 선 하중 모형으로 성공적으로 설명할 수 있음을 확인하였다. 이러한 결과는 원격 탐사 자료를 활용해 대규 모 하천의 수량 변동을 추정할 수 있을 뿐 아니라, 이를 토대로 GRACE가 관측하는 육상 물 저장량(terrestrial water storage, TWS)에서 토양 수분이나 지하수 변동 등의 개별 요소를 분리 및 검증할 수 있는 방법론을 제시한다. 나아가, 본 연구에서 제안된 접근법은 기후 변화로 인한 수문학적 재해 예측과 수자원 관리 등 다양한 분야에서 더욱 정교한 해석과 활용을 가능하게 할 것으로 기대된다.

초당옥수수는 고당도 옥수수 품종으로, 식단 트렌드 변화와 천연 당류에 대한 관심 증가로 인해 수요가 증가하면서 재배가 확대되고 있다. 초당옥수수는 높은 당도로 매우 달고 맛이 좋지만, 전분 함량이 낮아 일반 옥수수에 비해 발아율이 낮은 특징이 있다. 본 연구는 초당옥수수 계통의 전분 함량과 발아율의 상관관계를 조사하여, 우수한 생장과 발아율을 갖춘 고품질 계통을 선발하는 것을 목표로 하였다. 본 연구에서는 10개의 초당옥수수 계통을 대상으로 발아율과 식물체 생장 특성을 평가하였다. 모든 계통이 80% 이상의 발아율을 보였으며, KH51 계통이 53일로 가장 출사일이 빨랐고, KH29W 계통이 148cm로 간장이 가장 크게 나타났다. 고당도 계통을 선발하기 위해 수정 후 20일째에 이삭을 수확하여 유리당 함량을 측정하였다. KH28W 계통이 가장 높은 당 함량(23.3%)을 보였으며, KH45 계통이 가장 낮은 당 함량(8.1%)을 나타냈다. 또한, 전분 함량이 유리당과 비례하여 축적되는지를 확인하기 위해 수분 후 40일째에 이삭을 수확하여 분석한 결과, KH47 계통이 가장 높은 전분 함량(30.1%)을, KH28W 계통이 가장 낮은 전분 함량(23.6%)을 보였다. 전분 함량이 발아에 미치는 영향을 평가하기 위해 발아와 관련된 효소 및 유전자들을 분석하여, 발아 과정에서의 효소 활성 및 유전자 발현 수준을 측정하였다. 낮은 전분 함량은 발아 초기에 효소 활성과 유전자 발현을 저하시켜 발아 지연 및 불균일성을 초래하였으나, 발아 후 생장에는 큰 영향을 미치지 않았다. 이는 초당옥수수 종자의 초기 발아 과정이 유리당과 전분 함량에 의해 영향을 받을 수 있음을 시사한다.

Probiotics have been evaluated as therapeutic agents for cancer treatment in an increasing number of studies. This study investigated the inhibitory and cytotoxic effects of specific Lactobacillus strains on a human colorectal adenocarcinoma cell line (HT-29). The strains assessed were Limosilactobacillus (L.) reuteri VA 102, Ligilactobacillus (L.) animalis VA 105, and Limosilactobacillus (L.) reuteri KCTC 3594 (ATCC 23272). The viability of HT-29 cells was evaluated using the MTT assay. The findings revealed that cell-free supernatants (CFS) exhibited significant anticancer effects. Heat-inactivated L. reuteri VA 105 and L. reuteri KCTC 3594 induced a pronounced reduction in cell viability. Furthermore, live cultures of L. reuteri VA 105 and L. reuteri VA 102 also showed reduced cell viability compared to the control group. These results suggest that CFS and heat-inactivated cells may be more suitable for therapeutic applications than live bacteria owing to their improved safety profiles and reduced potential for adverse effects. Our findings also emphasize the potential anticancer benefits of these LAB strains.

Cell culture is a widely used in vitro tool that enhances our understanding of cell biology, disease mechanisms, drug responses, and the development of tissue engineering. However, there are a number of important drawbacks to conventional two-dimensional (2D) cultures, such as the loss of polarity, altered cell shape, and disruption of cell-extracellular matrix connections. Alternatively, organoids are tissue-engineered, cell-based in vitro models derived from stem cells that can self-organize and differentiate into three-dimensional (3D) structures, recapitulating the morphology and functions of their in vivo counterparts. Bisphenol A (BPA), a ubiquitous industrial chemical, has recently gained recognition as an environmental hazard. Previous research has demonstrated that BPA negatively affects the integrity of the intestinal barrier by triggering programmed cell death and suppressing cell growth in human colonic epithelial cell lines. However, a 2D-based cellular study cannot represent its exposure to multicellular organs. This work investigates the impact of BPA on the structure and function of the intestinal barrier. We examine the effect of BPA on the proliferation and tight junction gene expression with two models: the HT-29 colon cancer cell line and an intestine organoid model and morphological changes of intestinal organoid (I/O). The proliferation was increased in a dose-dependent manner with I/O, but at the same concentration, BPA does not increase the significant number of HT-29 cell respectively. Proliferation-related gene and tight junction gene expression pattern was similar between HT-29 and I/O other than Claudin-4. Therefore, this study offers a more precise depiction of the functional and morphological alterations caused by BPA in comparison to traditional 2D cell cultures.

포장상태 평가를 위한 노면영상 촬영은 라인스캔 방식이 주를 이루고 있다. 라인스캔 특성 상, 조사환경이나 장비특성이 달라질 경 우 밝기가 상이한 노면영상을 취득할 수 있고 이는 U-net과 같은 픽셀 단위 segmentation 딥러닝 모델의 균열 자동검출 성능에 영향을 미친다. 본 연구에서는 인공지능 검출 모델의 변경 없이 영상의 밝기 최적화와 morphology 연산기법을 노면영상 전·후처리 방법으로 제시하고 그 효과를 분석하였다. 영상 처리를 통해 과다 검출경향을 보인 이상치들이 제거되었으며 정답으로 간주할 수 있는 전문요 원 분석결과인 GT 균열률과의 상관성 또한 향상됨을 확인하였다.

Line observations of young stellar objects (YSOs) at (sub)millimeter wavelengths provide essential information of gas kinematics in star and planet forming environments. For Class 0 and I YSOs, identification of Keplerian rotation is of particular interest, because it reveals presence of rotationally-supported disks that are still being embedded in infalling envelopes and enables us to dynamically measure the protostellar mass. We have developed a python library SLAM (Spectral Line Analysis/Modeling) with a primary focus on analyses of emission line data at (sub)millimeter wavelengths. Here, we present an overview of the pvanalysis tool from SLAM, which is designed to identify Keplerian rotation of a disk and measure the dynamical mass of a central object using a position-velocity (PV) diagram of emission line data. The advantage of this tool is that it analyzes observational features of given data and thus requires few computational time and parameter assumptions, in contrast to detailed radiative transfer modelings. In this article, we introduce the basic concept and usage of this tool, present an application to observational data, and discuss remaining caveats.

본 연구는 온라인 플랫폼을 활용한 건강사정 학습 프로그램이 간호 대학생의 문제해결과정, 비 판적 사고 성향, 수업참여도 및 수업몰입도에 미치는 효과를 알아보기 위한 단일집단 사전사후설계 (one-group pretest-posttest design)를 적용한 원시실험연구(pre-experimental research)이다. 연구 대상자 는 C북도 G군 소재 J대학교 건강사정 및 실습 교과목을 수강하는 간호학과 2학년 학생 52명을 대상으로 온라인 플랫폼을 활용한 건강사정 학습 프로그램 참여 전‧후 자료를 수집하였다. 분석한 결과, 문제해결과 정(t=-2.569, p=.013), 비판적 사고 성향(t=-5.363, p<.001), 수업참여도(t=-4.429, p<.001), 수업몰입도 (t=-3.747, p<.001)가 프로그램 참여 전‧후 통계적으로 유의하게 향상된 것으로 나타났다. 따라서 건강사정 수업 시 간호 대학생들의 문제해결과정, 비판적 사고 성향, 수업참여도, 수업몰입도 향상을 위해서 온라인 플랫폼을 활용한 학습 프로그램을 활용할 수 있을 것으로 사료된다.

낫또(natto)는 일본의 전통 콩 발효식품으로 혈전용해, 면역증강, 항고혈압, 항비만, 항균, 항산화, 항당뇨등의 다양한 효과를 가지고 있어 소비가 점차 증가하고 있다. 그러나, 낫또용으로 이용되는 콩 품종의 성숙 종실에는 소화 불량, 알러지 유발 및 가공 적성을 저하시키는 P34, lectin, Kunitz Trypsin Inhibitor (KTI), 7S α′subunit 및 lipoxygenase 단백질과 같은 항영양성분도 포함되어져 있다. 따라서, 본 연구는 이러한 성분에 대하여 penta null 유전자형(p34leticgy1lox1lox2lox3)을 가지면서 종자 크기가 중소립이고 노란색 종피와 제색이 노란 또는 옅은 갈색을 가진 낫또용 콩 계통을 육성하기 위하여 진행되었다. 64 및 22C1 모본과의 교배를 통하여 제색이 노란 및 옅은 갈색이면서 종자 크기가 소립인 81개의 F2 종자가 선발되었고 그 중에서, P34 단백질 함량이 매우 낮은 18개의 F2 종자가 선발되었다. 18개의 F2 종자로부터 초형, 성숙기, 종피색, 제색 및 백립중이 양호한 2개의 F2 개체(BL1 및 BL2)가 최종 선발되었다. BL1 선발 개체의 성숙기는 중간이었고 종피색은 노란색이며 제색은 연한 갈색을 보였고 백립중은 12.4g으로 소립이었다. BL2 선발 개체는 성숙기가 조생이었고 종피색과 제색은 모두 노란색은 보였으며 백립중은 12.6g으로 소립이었다. 선발된 두 개체는 모두 대표적인 항영양성분인 P34, lectin, KTI, 7S α′subunit 및 lipoxygenase 단백질에 대하여 모두 열성인 penta null 유전자형(p34leticgy1lox1lox2lox3)을 가진 것으로 확인되었다. 선발 개체는 낫또용 콩 품종으로 육성될 수 있을 것으로 사료되었다.

This study improved the work efficiency by supplementing the shortcomings of the manual process by developing a double tube feeding device, and the following results were obtained by conducting the production capacity, production length, and defect rate tests. Developed a double tube production system to enable the simultaneous production of two tubes, increasing the production volume by about 1.5 times. The product length has been improved from semi-automatic to automated, and the production capacity has been improved from 16 to 25 pieces per hour (based on 15m). Developed a double-tube input straight line automatic adjustment feeder, which resulted in reducing the defect rate to less than 1%.

Background: Most non-pharmacological interventions for tension-type headache (TTH) focus on direct intervention in areas associated with headaches, with limited research exploring the indirect effects of interventions utilizing the fascia. Objectives: To investigate the effects of superficial back line (SBL) stretching and head-neck massage interventions on the range of motion (ROM) and neck disability index (NDI) of TTH. Design: Randomized controlled trial. Methods: The study participants were randomly allocated into three groups: SBL stretching group (n=9), head-neck massage group (n=9), and combined group (n=9). Prior to the intervention, measurements were taken for neck flexion ROM, straight leg raising test (SLRT) ROM, and NDI. The intervention consisted of 30-minute sessions conducted twice a week for 8 weeks. The second measurement took place 4 weeks after the intervention, followed by a third measurement conducted after an additional 4 weeks of intervention. Results: Comparing neck flexion ROM within each group, a significant increase was observed in the SBL stretching group after 8 weeks compared to before the intervention (P<.05). Regarding the comparison of SLRT ROM within each group, a significant increase was noted after 8 weeks compared to before the intervention in the SBL stretching, head-neck massage, and combined groups (P<.05). In terms of the comparison of NDI within each group, a significant decrease was observed in all three group after the intervention compared to before the intervention (P<.05). Conclusion: 8-week intervention involving SBL stretching, head-neck massage, and their combined application demonstrated effectiveness in improving neck flexion ROM, SLRT ROM, and the NDI for individuals with TTH.

Uncoupling protein 1 (UCP1) is a unique mitochondrial membranous protein expressed in brown adipose tissue (BAT) in mammals. While its expression in response to cold temperatures and adipogenic inducers is well-characterized in mammals and human infants, the molecular characterization and expression of UCP1 in fish remain unexplored. To address this gap, we analyzed UCP1 expression in response to adipogenic inducers in a fish cell line, rainbow trout gonadal cells (RTG-2), and compared it with UCP1 expression in three mammalian preadipocytes, 3T3-L1, T37i, and WT1 exposed to the Peroxisome proliferator-activated receptor gamma (PPARγ) agonists, rosiglitazone (Rosi). In mammalian preadipocytes, UCP1 protein was highly expressed by Rosi, with an induction of adipogenesis observed in a time-dependent manner. This suggests that UCP1 plays a significant role in adipogenesis in mammals. However, RTG-2 cells showed no response to adipogenic inducers and exhibited only marginal expressions of UCP1. These results imply that RTG-2 cells may lack crucial responsive mechanisms to adipogenic signals or that the adipogenic response is regulated by other mechanisms. Further studies are needed to confirm these phenomena in fish preadipocytes when an appropriate cell line is established in future research.

Application areas of floating marine structure systems have been increased with the development of power generation systems using renewable energy. Hence it is necessary to analyze the behavior of these floating systems for efficient design and operation. In this study, a computational analysis was performed to predict the characteristics of mooring lines load variation connected to a floating marine structure with waves. Pressure on the floating body and mooring lines load were analyzed with wave direction and height. The floating body stability severely decreased for 90° of the wave incident direction, and maximum load of the mooring lines increased with the height. These results are expected to be applicable for optimal design of the marine floating system.

It has been a consensus among academic circles that the Ten-Line Edition of Da Guang Yi Hui Yu Pian from the Imperial House Library of Japan is “Song Dynasty carved and Yuan Dynasty revised”, but each scholar lacks a detailed judgment basis. The investigation of the broken line of the column frame and the engraving artist’s name as well as the proofreading with the same version system of Song version (remaining pages) from Kanazawa Bunko, can provide a partial basis for where is “Song carving” and where is “Yuan repair” in the Archives and Mausolea Department collection, and correct the mistakes made by predecessors in the judgment process. The vulgar character in the name of the engraving artist advanced the appearance of liu (刘) in paper documents to the middle of the Southern Song Dynasty.

To identify some significant phenotypic characteristics of maize(zea mays) seeds, we have obtained Red, Green, Blue(RGB) digital image data from 82 recombinant inbred lines. Based on the collected image data, their morphological and color data were analyzed, and seven significant parameters were selected, including area, perimeter, length, width, circularity, roundness, and surface texture. The extracted RGB data were converted into color hex codes to visualize the representative colors of the seeds. These visualized colors were categorized into six groups: gray, yellowish white, yellow, grayish orange, purple, and brown. The results of maize seed phenotypic analysis using the RGB digital images in this study will serve as a useful tool for constructing a database of seed phenotyping database and establishing a standardized classification system.