This study was conducted to determine the agronomic characteristics of low amylose lines which were derived from induced mutants by T-DNA insertion. The agronomic and physicochemical properties of the low amylose mutants were analyzed and compared with a donor cultivar 'Dongjin' and a low amylose cultivar 'Baekjinju'. The heading date of the low amylose mutants was similar to the donor cultivar 'Dongjin' and yield index of the mutants was 78~92% compared with 'Dongjin'. The amylose content of four mutants in brown rice was ranged from 16.1 to 16.7%. Among low amylose mutants, 'P50-4-4-5' was lower 3.6%(13.3%) than those of 'Dongjin'(16.9%) in amylose content of milled rice. The grain length of 'P50-4-4-5' was similar to the donor cultivar, however, thousand grain weight(18.9g) was lighter than those of 'Dongjin'. The score of alkali digestion in brown rice of 'P50-4-4-5'(5.5) was lower than that of 'Dongjin'(6.8) and similar to 'Baekjinju'(5.8). The gel consistency of 'P50-4-4-5'(84mm) in milled rice exhibited that was longer than 'Dongjin'(76mm) and 'Baekjinju'(81mm). The result of eating quality showed that 'P50-4-4-5'(78.8) was higher than those of 'Dongjin'(60.3) and 'Baekjinju'(67.2). Thus our data suggest that 'P50-4-4-5' will facilitate the development of a new cultivar with low amylose rice.

The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (x2 = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.

저글루테린 및 저아밀로스 품종인 ‘건양2호’는 ‘중국173 호’와 ‘주남벼’를 각각 모본과 부본으로 인공교배 후 집단육 종법을 통하여 육성한 기능성 품종이다. ‘건양2호’의 출수기 는 영호남 및 중부평야지 보통기 보비재배에서 8월 10일로 ‘남평벼’보다 5일 빠른 중생종이다. 간장은 65 cm로 내도복 성이며, 불시출수와 수발아는 안되는 편이고 내냉성은 ‘남평 벼’와 비슷한 편이다. 잎도열병 밭못자리검정에서 중정도 저항성을 보였으며, 줄무늬잎마름병과 흰잎마름병(K1, K2, K3) 에는 저항성이다. ‘건양2호’의 아밀로스함량은 11.5%로 메벼 와 찹쌀의 중간형태로 불투명한 저아밀로스 품종이며, 단백질 함량은 6.0%로 ‘남평벼’보다 약간 낮으며 소화성 전분인 글 루테린 비율이 63.1%로 ‘남평벼’의 73.8%보다 낮다. ‘건양2 호’의 쌀 수량성은 4.61 MT/ha로 ‘남평벼’보다 9% 감수되었 고, 적응지역은 영호남 및 중부 평야 1모작지이다(품종 등록 번호: 제5105호).

“Joongmo2012”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Sinmichal/Keumkang” during 2006. “Joongmo2012” was evaluated as “Iksan357” in advance yield trial test in 2010. It was tested in the regional yield trial test between 2011 and 2013. Its heading date was May 2 in upland and May 1 in paddy filed conditions, and Maturing date was June 8 in upland and June 8 in paddy field, which were similar to Keumkang, respectively. It showed 628 in number of spikes per m2, 34 of grain number per spike, 47.6g of 1,000 grain weight, and 762g of test weight. “Joongmo2012” showed susceptible to Fusarium head blight (Scab) in test of specific character although “Keumkang” is susceptible to scab. “Joongmo2012” had lower flour yield (68.4%) than “Keumkang” (73.5%, respectively). “Joongmo2012” showed similar ash (0.40%), lower protein content (11.7%), SDSsedimentation volume (39.8ml) and gluten content (8.1%) than “Keumkang” (0.41%, 12.7%, 55.8ml and 9.2%, respectively). It showed higher lightness (92.70) in flour color than “Keumkang” (91.51, respectively). “Joongmo2012” exhibited lower hardness (3.19N), similar springiness and higher cohesiveness of cooked noodles (0.90 and 0.66) compared to “Keumkang” (4.21N, 0.90, and 0.62, respectively). The average grain yield in the regional yield trial was 5.48MT/ha in upland and 4.47 MT/ha in paddy field, which were higher 5% and similar than those of the check cultivar, Keumkang, respectively

‘진상’은 공주대학교에서 2013년도에 육성한 고품질 저아밀로스 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1.‘진상’은 중서부평야 보통기 보비재배에서 출수기가 8월 11일로 ‘추청’보다 약 11일 빠른 중생종으로, 이삭길이는 19 cm로 ‘추청’과 비슷한 수준이었으나, 간장은 75.2 cm로 ‘추청’보다 짧다. 2.‘진상’의 정현비율은 81.2%로 ‘추청’과 비슷한 수준이며 현미길이와 폭이 각각 4.86 mm, 2.91 mm로 ‘추청’에 비해 약간 길고, 넓은 단원형 품종이다. 현미 천립중은 20.91 g 수준이며, 수량성은 457 kg/10a로 ‘추청’과 비슷하였다. 3.‘진상’은 중간찰벼로서 투명도가 중간 정도의 불투명 배유 특성이고, 알칼리 붕괴도는 6.4 정도이며, 아밀로스함량은 11.9%로 대조품종 19.1%보다 낮고, 도요 식미기로 측정한 밥 윤기치는 대조품종인 ‘추청’보다 높게 나타났다.

“Hojoong”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA, Iksan, Korea, during the period from 2006 to 2012. The heading and maturing dates of this variety were May 2 and June 10 in upland, and May 3 and June 7 in paddy field, respectively. It is an It is an awned, semi-dwarf and soft red winter wheat. Culm and spike length of “Hojoong” were 79cm and 9.1cm. It had lower test weight (803 g/ℓ) and 1,000 grain weight (39.5g) than “Keumkang”. It showed resistance to pre-harvest sprouting, which lower rate of pre-harvest sprouting (5.5%) than “Keumkang”. “Hojoong” had lower flour yield (66.7%) and ash content (0.38%) than “Keumkang”. It showed lower protein content (11.3%) and lower SDS-sedimentation volume (34.0㎖) and amylose content (20.5%) than “Keumkang”. It showed higher high viscosity (204BU) and lightness of noodle dough sheet (80.63). It showed different composition in HMW-GS compositios (2.2+12), PPO18 (876bp), GBSS B (null type) and Puroindolines composition compared th “Keumkang”. Average yield of “Hojoong” in the regional adaptation yield trial test was 5.51 ton/ha in upland and 5.10 ton/ha in paddy field. “Hojoong” would be suitable for the area above –10℃ of daily minimun temperature in January in Korean peninsula

‘설백’은 조숙 내병 고품질 중간찰 계통을 육성할 목적으로 2000년 하계에 중간찰 품종인 수원460호에 조숙 내병 고품질 품종인 그루벼를 인공교배하여 2010년 육성된 조생 내도복 뽀얀 중간찰이다. ‘설백’은 보통기 보비재배에서 출수기는 평균 7월 29일로 ‘오대벼’보다 3일 빠른 조생종이다. ‘설백’의 간장은 60 cm이며, 주당수수는 13개, 수당립수는 81개이고, 천립중은 20.7 g으로 오대벼보다 가볍다. ‘설백’의 아밀로스함량은 10.0%로 중간찰이고 쌀 외관은 투명도가 5로 불투명해 보인다. ‘설백’은 위조에 강하고 불시출수가 잘 되지 않으며 성숙기에 하엽 노화가 느린 편이다. ‘설백’은 수발아에 강한 편이고, 내냉성은 ‘오대벼’와 비슷한 수준이다. 잎도열병에는 강한 저항성이고 흰잎마름병, 바이러스병 및 해충에는 약하다. 지역적응시험 보통기 보비재배에서 평균 쌀 수량이 4.96 MT/ha로 ‘오대벼’ 대비 92%의 수량성을 보였다. 재배적지는 중부평야지, 북부평야지, 중산간지, 남부고랭지 및 동북부해안지이다.

‘월백’은 조숙 내병 고품질 중간찰 계통을 육성할 목적으로 2000년 하계에 중간찰 품종인 ‘Milkyqueen’에 조숙 내병 고품질 품종인 ‘그루벼’를 인공교배하여 2010년 육성된 조생 내도복 반투명 중간찰 품종으로 주요 특성을 요약하면 다음과 같다. ‘월백’의 보통기 보비재배에서 출수기는 평균 7월 31일로 ‘오대벼’보다 1일 늦은 조생종이다. ‘월백’의 간장은 67 cm이며, 주당수수는 16개, 수당립수는 59개이고, 현미천립중은 21.4 g 으로 ‘오대벼’보다 가볍다. ‘월백’의 아밀로스함량은 11.6%로 중간찰이고 쌀 외관은 투명도가 4로 반투명해 보인다. ‘월백’은 위조에 강하고 불시출수가 잘 되지 않으며 성숙기에 하엽 노화가 느린 편이다. ‘월백’은 수발아에 강한 편이고, 내랭성은 ‘오대벼’와 비슷한 수준이다. ‘월백’은 잎도열병에는 강한 저항성이고 흰잎마름병, 바이러스병 및 해충에는 약하다. 지역적응시험 보통기 보비재배에서 평균 쌀 수량이 5.13 MT/ha로 ‘오대벼’ 대비 95%의 수량성을 보였다. ‘월백’의 재배적지는 북부평야지, 중산간지, 남부고랭지 및 동북부해안지이다.

Amylose content of rice endosperm is one of the determinants of rice eating quality. This study was conducted to elucidate the mode of inheritance of dull gene in Milyang262, tentatively designated as du7(t), and to identify the molecular marker for du7(t) to be employed in marker-assisted breeding and gene pyramiding. Genetic analysis was carried out on F2 population derived from a cross between Junam and Milyang262. The low amylose content of Milyang262 was indicated to be under single recessive control. Allelism tests were as well conducted by crossing Milyang262 with Baegjinju and Baegokchal, which harbor du1 and wx gene, respectively. du7(t) was demonstrated to be inherited independently to du1 and wx. F2 population of Baegokchal/Milyang262 was used for molecular mapping. Linkage analysis was conducted on a population consisted of 120 individuals by several SSR markers. Initial mapping indicated that du7(t) is located on the end of long arm of chromosome 6 between SSR marker RM20590 and RM3509. To fine map the gene, a bigger population and several additional markers were employed. du7(t) was further mapped to a 1.74 Mb region between two SSR markers (RM6926 and RM412). Furthermore, we indentified three SSR markers that co-segregated with du(t) i.e. RM6811, RM3765, and RM176.

‘만미벼’는 작물과학원 영남농업연구소(현 국립식량과학원 기능성작물부)에서 2002년도에 육성한 중만생 저아밀로스 반찹쌀 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 출수기는 남부평야지의 보통기재배에서 8월 16일로 ‘동진벼’ 보다 2일 빠른 중만생종이다. 2. 수당립수는 ‘동진벼’ 보다 18개 많으나, 현미천립중은 19.9 g으로 ‘동진벼’ 보다 가벼운 편이며 등숙비율이 약간 떨어진다. 3. 위조현상에 강하고 성숙기 엽노화가 느린 편이며 내