강황 분말의 첨가가 마요네즈의 품질특성, 항산화활성 및 산화안정성에 미치는 영향에 대해 평가하였다. 들기름으로 제조된 마요네즈에 강황 분말을 0.5%, 1%, 2%, 3% 농도로 첨가하여 4°C에서 12주 동안 저장하였으며, 강황 분말 무첨가구와 비교하였다. 강황 분말 첨가량이 많아짐에 따라 들기름 마요네즈의 L* (명도) 값은 유의적으로 감소하였고, a* (적색도)는 모든 실험구에서 negative (-)값이었으며, b* (황색도)값은 유의적으로 증가되는 경향이었다(p<0.05). 강황 분말 첨가량이 많아짐에 따라 총 페놀 함량 및 항산화 활성이 유의적으로 증가하였다(p<0.05). 마요네즈를 저장하는 동안 과산화물가는 유의적으로 증가하였으나, 강황 분말 첨가 시료에서는 유의적으로 낮았다(p<0.05). 마요네즈의 유화안정성은 저장 초기에 비해 저장 기간이 길어질수록 감소되는 경향이었으나, 0.5%의 강황 분말 첨가구는 무첨가구에 비해 유의적으로 높은 유화안정성을 보였으며, 1%의 강황 분말 첨가구는 무첨가구와 유의차를 보이지 않았다. 점도는 저장 기간이 경과됨에 따라 감소되었으나, 무첨가구에 비해 1% 이상의 강황 분말 첨가구에서 유의적으로 높았다. 들기름 마요네즈에 강황 분말 첨가 시 항산화 활성 증대 및 과산화물의 생성 억제에 효과적이었으며, 특히 0.5~1% 첨가시 마요네즈의 유화안정성 및 점도 개선에 도움이 될 것으로 판단된다.
The wellness travel industry faces a curious challenge: it experiences high consumer demand for indigenous experiences while also facing growing accusations of cultural appropriation of authentic practices. According to the Global Wellness Summit (2023), “hyper-indigenous” wellness travel is a global trend for 2023, as travelers seek the source of ancient healing knowledge. However, many of these cultural practices have already spread across national borders like the turmeric latte served in Western retreats or yoga that is practiced globally, leading to an established yoga tourism market (Market Research, 2022). How can these conflicting realities, rooted in the concept of authenticity, be reconciled? This study aims to explore this research question by analyzing two wellness narratives: one that commodifies ingenious practices outside their original borders and another that safeguards them within national and cultural boundaries.
This study was conducted to investigate the effect of manufacturing temperature on curcuminoid contents and the antioxidant activity of turmeric (Curcuma aromatica Salab.) powder-enhanced nurungji. Two different processes employed turmeric powder (2%): AR (added after rice cooked) and BR (added before rice cooked). AR and BR nurungji samples were prepared by heating cooked rice at 180oC, 200oC, and 220oC for 3 minutes. As a result of the HPLC analysis, the contents of curcumin, demethoxycurcumin, and bisdemethoxycurcumin decreased as the heating temperature increased. Moreover, AR samples showed higher curcuminoids than BR samples. The total polyphenol contents, DPPH, and ABTS radical scavenging ability proportionally increased with the increase of the manufacturing temperature. On the other hand, flavonoid contents were decreased with increasing temperature. These results suggest that the preparation of turmeric powder-enhanced nurungji by AR process at 200oC is preferable to preserving curcuminoid contents and antioxidative activity.
The purpose of this study is to optimize the composition of the medium for turmeric fermentation and to select competent turmeric fermentation strains using bacterial isolates from kimchi. Initially, 30 isolates from kimchi were cultured in 5% (w/v) yeast extract and 1% (w/v) maltodextrin to determine viability. As a result, eight strains showed a tendency to maintain viability until the fifth day of fermentation. Subsequently, the eight isolates were fermented in an optimum medium for turmeric fermentation, 5% (w/v) yeast extract, 1% (w/v) maltodextrin, and 5% (w/v) turmeric for seven days to determine the viable cell count and antioxidant capacity. The antioxidant capacities of turmeric fermented by the eight isolates were similar or higher than turmeric fermented by Lactococcus lactis KCTC 2013, while maintaining high viable cell counts of both the eight isolates and L. lactis KCTC 2013 until the seventh day of fermentation. The antioxidant capacities of the selected five strains during fermentation might increase possibly due to the biological conversion of active compounds in turmeric by fermentation. Consequently, a total of five strains of the isolates showing higher antioxidant capacity (4.81±0.19-5.81±0.04 VCE/mL) than fermentation day 0 were selected for fermentation of turmeric.
The antioxidant, physicochemical, and sensory properties of muffins using fermented Curcuma longa L. were investigated. Sensory properties of the samples were examined, and the correlation between the physicochemical, antioxidant, and sensory properties was studied. The pH of the samples was pH 7.26-7.42, which was the optimal pH range for the air formation of the dough, color, or volume. The antioxidant activities of the sample group were significantly higher than the control (p<0.05). In the sensory intensities, the 1.0 and 1.5%-added sample groups showed a similar savory aroma, sweet aroma, savory taste, sweet taste, and texture to those of the control. Samples containing 1.0 and 1.5% showed similar overall acceptance values to the control. The muffin containing 1.0 or 1.5% fermented Curcuma longa L. powder was developed successfully, and it was concluded that the developed muffin with the fermented turmeric powder could be a highly valued product in the increasingly competitive muffin food industry.
울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향을 확인하고 기능성 식품 소재로 활용 가치를 검토하기 위하여 연구를 수행하였다. 총 카로티노이드(total carotenoid) 함량은 1.581±0.005 mg β-carotene equivalents (BCE)/g dry weight으로 관찰되었다. 70% 메탄올, chloroform:methanol (CM, 2:1, v/v), 에틸 아세테이트(ethyl acetate, EA) 3가지 용매를 사용한 추출 수율은 70% 메탄올(16.54%), CM (5.64%), EA (4.14%) 순으로 나타났다. 총 페놀 함량은 EA, CM 및 70% 메탄올에서 각각 106.287, 90.614 및 18.527 mg gallic acid equivalents (GAE)/g의 함량으로 나타났으며, 추출 용매 별 항산화능은 0.2, 0.4, 0.6, 0.8 mg/mL의 농도로 측정하였고, 양성대조구로 사용된 BHA (butylated hydroxyanisole) 및 trolox 보다는 낮은 항산화능을 보였다. Nitric oxide (NO) 라디칼 소거능은 70% 메탄올에서 28.65~48.43%, CM 18.86~55.10%, EA에서 15.68~56.25%로 관찰 되었다. Nitrite (NO2) 소거능은 70% 메탄올, CM 및 EA 순으로 나타나 EA 추출물에서 유의적인 차이를 보이며 강한 NO2 소거능을 나타내었다(p<0.05). β-carotene 탈색 저해능은 70% 메탄올, CM 및 EA에서 각각 1.64~23.79%, 6.99~41.16% 및 10.20~48.52%로 관찰되었다. 한편, 지질과산화 저해능은 70% 메탄올, CM 및 EA 추출물에서 높게 측정되었다. 이상의 결과로 미루어 볼 때, 울금은 기능성 식품 소재로서 활용 가치가 있을 것으로 판단된다.
Streptozotocin (STZ, 45 mg/kg body weight)으로 유도된 Sprague Dawley (SD)계 수컷 흰쥐에 5% 울금을 섭취시켰을 때, 혈청 크레아티닌(creatinine) 및 blood urea nitrogen (BUN)의 농도와 alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aminotransferase (AST, ALT), 카탈라아제(catalase), 아밀라아제(amylase) 및 리파아제(lipase) 활성에 미치는 영향을 알아보기 위해 본 실험을 수행한 결과, 기본식이를 급여시킨 대조군(control)인 정상군은 BD군, 기본식이 실험군에 5% 울금 급여군(BT군), 당뇨 유발 실험군(BS군)인 질환 모델 대조군(control-diabetic)과 질환 실험군(diabetic, BS군) 에 5% 울금을 급여시킨 실험군(ST군)으로 나누었다. 흰쥐의 혈청 크레아티닌 및 BUN 농도와 ALP, AST 및 ALT 활성은 유사한 경향으로 5% 울금의 급여가 감소시킨 것으로 확인되었다. SD계 수컷 흰쥐의 혈청 LDH 활성은 BS군에서 유의적인 차이를 나타내며 높은 활성을 보였으나(p<0.05), 5% 울금의 급여가 감소 효과를 나타내었다(p<0.05). 카탈라아제 활성은 BS군에서 유의적으로 낮은 활성을 나타내었고, ST군에서 활성을 증가시켰다(p<0.05). 혈청 아밀라아제 및 리파아제의 활성은 5% 울금의 급여가 감소시킨 것으로 확인되었다. 본 연구 결과, SD계 수컷 흰쥐를 통한 in vivo 실험에서 5% 울금의 섭취가 당뇨, 내인성 항산화 효소 및 간 기능 개선에 효과가 있는 것으로 나타났다.
울금(Curcuma longa L.)이 이상지질혈증 유발 흰쥐의 지질성분과 단백질 및 전해질 농도에 미치는 영향을 확인하기 위하여 기본 식이를 섭취시킨 ND군, 정상 실험군에 5% 울금 급여군(NT군), 이상지질혈증 유발 실험군(DD군)과 DD군에 5% 울금을 섭취시킨 실험군(DT군)으로 나누어 실험을 행하였다. 혈청 지질성분(총 콜레스테롤, LDL-콜레스테롤, 중성지질, 인지질, 유리 콜레스테롤, 콜레스테롤 에스테르), 혈당 및 유리지방산(non esterified fatty acid, NEFA)의 농도와 동맥경화지수 (atherosclerotic index, AI), 심혈관 위험지수(cardiac risk factor, CRF)는 이상지질혈증군에서 여타 실험군과 비교하였을 때 유의적인 증가를 나타내었으며(p<0.05), 5% 울금 급여군에서 감소시키는 것으로 나타났다. DD군에 비해 DT군에서 고밀도 지단백 콜레스테롤(high density lipoprotein-cholesterol, HDL-콜레스테롤) 및 총 콜레스테롤에 대한 HDL-콜레스테롤 농도 비는 유의적인 증가를 보였다 (p<0.05). 혈청 단백질의 농도에 있어서, DT군은 DD군에 비하여 흰쥐의 혈청 알부민(albumin) 농도와 알부민/글로불린 비(albumin/globulin ratio, A/G 비)의 증가 및 글로불린(globulin) 농도는 감소되는 것으로 관찰되었다. 나트륨(Na) 및 염소(Cl)는 DT군에서 DD군 보다 농도가 감소된 것으로 확인하였다. 이상의 결과, 5% 울금은 흰쥐의 혈청 지질성분 개선과 단백질 및 전해질 농도 조절에 도움이 되는 것으로 사료된다.
울금(Curcuma longa L.)의 사포닌 함량과, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올의 3가지 용매를 사용하여 산화억제 효과를 통한 기능성 식품 소재로서의 가치를 확인한 결과, 총 사포닌 함량은 7.506±0.349 mg SE/g dry weight으로 확인되었다. 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%)로 DW에서 가장 높게 나타났으며, 노르말 부탄올 추출물에서 낮은 수율을 보였다. 항산화 활성은 농도가 높아질수록 활성이 증가되는 것으로 관찰되었다. 총 페놀 함량은 DW, 70% 에탄올, 노르말 부탄올 순으로 각각 8.57, 41.01, 119.11 mg GAE/g의 함량으로 나타났으며, 노르말 부탄올 추출물에서 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). DPPH 라디칼 소거활성은 DW, 70% 에탄올, 노르말 부탄올 순으로 확인되었다. ABTS 라디칼 소거활성, 환원력 및 ferric reducing antioxidant power (FRAP) 또한 DPPH 라디칼 소거활성과 비슷한 양상으로 나타났지만, ferrous ion 킬레이트 능은 노르말 부탄올(15.87~21.17%), 70% 에탄올(26.10~50.76%), DW(44.47~84.24%) 순으로 측정되었다. 이에, 울금은 산화억제 및 생리활성을 가지는 것으로 규명되었으며 기능성 식품 소재로서의 활용 가능성이 높은 것으로 사료된다.
Nosema disease caused by the microsporidia Nosema apis and Nosema ceranae are a honey bee pathogen parasitizing. Nosema disease symptoms include digestive and absorption disorders because the spores damage epithelial tissue and potentially causing colony death. Recently, N. ceranae has been reported as an important threat to honey bee health. Turmeric (Curcuma longa L.) Curcuma tonga L. belongs to the family Zingiberaceae and is a perennial, tropical herb. Turmeric, the powdered rhizome, is used for medicinal purposes. The aim of this study was to evaluate the potential of Turmeric (Curcuma longa L.) for the control of N. ceranae in honeybees. For the study, we infected with N. ceranae spore through dosed and fed with the turmeric extraction at difference concentration. The data show that the turmeric extraction was not toxic for bee at least at 1% and the bees fed with 0.5 % turmeric extraction had significantly lower infection rates. This data suggest that turmeric could be useful in alternative strategies for the control of N. ceranae.
Turmeric is known for its ability to enhance immunity via anti-inflammatory and anti-oxidant effects. Salmonella enterica species contain a large number of pathogenic serotypes that are adapted to a broad range of vertebrate hosts. Our previous study revealed that bioprocessed polysaccharides from the liquid culture Lentinus edodes fungal mycelia containing turmeric (BPP-turmeric) is able to alter chicken macrophage responses and increases chick survival against Salmonella enterica infection. In this study, we examined the immunomodulatory effects of BPP-turmeric on the porcine macrophage 3D4/31 cell line infected with Salmonella enterica subsp. enterica serovar Choleraesuis (S. Choleraesuis) or S. Enteritidis. Our experimental analyses demonstrated that BPP-turmeric (i) does not alter phagocytic and killing activity of 3D4/31 against either Salmonella serotypes, but that it (ii) represses mRNA transcription of interleukin (IL)-6, IL-8, and tumor necrosis factor α in response to Salmonella infection. Collectively, these results imply that BPP-turmeric has an immunomodulatory effect that represses pro-inflammatory cytokine expression in porcine macrophages, suggesting that it may protect swine from salmonellosis via controlling Salmonella-induced hyperinflammation.
In this experiment, we determined the effect of curcumin supplementation in freezing buffer for miniature pig sperm cryopreservation. Each ejaculate was diluted with modified Modena B extender and mixed with lactose-egg yolk (LEY extender, 80% v/v lactose solution [310 mM], 20% v/v egg yolk, and100 μg/mL kanamycin sulfate) and LEY-glycerol Orvus ES Paste (LEYGO, 89.5% v/v LEY, 5% v/v glycerol, 1.5% v/v Orvus ES Paste), 100 mM trehalose supplemented with 0, 10, 50, 100, and 500 μM of curcumin from turmeric, respectively. Following equilibration, the 0.5 mL French straws were frozen and plunged into LN2 tank for 7 days at least. Sperm parameter and oxidative byproducts were determined by the computer assisted sperm motility analysis (CASA) and fluorescence-activated cell sorting (FACS) as compared with each groups.Supplementation of curcumin had no effect on sperm motility, progressive motility and curvilinear velocity. However, average-path velocity and straight-line velocity were significantly higher in 10 μM curcumin group (100.9±8.8 μm/s, 61.7±2.9 μm/s, respectively) than control group (77.8±3.9 μm/s, 46.4±3.0 μm/s, respectively) (p < 0.05). In addition, the level of the O2 radical and H2O2 were comparatively decreased in curcumin groups by evaluation of ethidium and DCF fluorescence. According to the results, curcumin can improve sperm kinetic variables and alleviate ROS induced cryoinjury to pig sperm.
Nanoemulsions containing Turmeric extract were fabricated using ultrasonication (US) system and their physicochemical properties were characterized by mean droplet size, size distribution, zeta potential, and morphology. Turmeric was firstly extracted using 50% ethanol with heating and concentrated to increase the solid content. The final curcumin content in the concentrated turmeric extract was 10.4 mg/mL. Medium chain triglyceride (MCT) oil was selected as an oil phase by the solubility test. Turmeric extract-loaded nanoemulsions (TE-NEs) were prepared with oil phase containing lecithin and water phase containing tween 80 by ultrasonication treatment. The mean droplet size of TE-NEs was significantly decreased with ultrasonication time and ranged from 66 nm to 279 nm. The optimum HLB (hydrophilic lipophilic balance) value was 10.6 which decided to obtain the smallest droplet size and the highest zeta potential of TE-NEs. TE-NEs showed good storage stability at 4°C for 30 days without any phase separation and significant change of both mean droplet size and zeta potential. Transmission electron microscope (TEM) images support that the droplet of TE-NEs was individually spherical shape and not aggregated or agglomerated until the TE concentration was less than 500% (w/w MCT oil).
The antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from turmeric (Curcuma longa L.) was investigated in this study. The antioxidant potential of various extracts of turmeric was evaluated by using different antioxidant tests, namely DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS [2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ferric reducing antioxidant power and β-carotene bleaching assays as well as total phenol content and total flavonoid content. All extracts exhibited abundant total phenol content, total flavonoid content and strong antioxidant activity. Particularly, higher activity was exhibited by CM extract with 46.32±0.35 mg CAE/g and 15.58±0.39 mg QE/g of total phenol content and total flavonoid content. Generally, antioxidant activity of various extracts from turmeric increased in the following order: 70% methanol extract, 70% ethanol extract and CM extract, respectively. Results suggested that turmeric could be widely used as a source of antioxidant and a kind of functional material in food.
Pathologic chronic inflammation, such as that seen with microbial infection and autoimmune diseases, creates microenvironmental conditions that promote cancer. Therefore, if chronic inflammation can be alleviated, the risk of carcinoge- nesis may decrease. Turmeric is a dried rhizome powder from Curcuma longa. Curcumin, the major constituent of turmeric, presents anti-inflammatory, antioxidant, antimicrobial and chemopreventive activities. In the present study, we investigated immune responses to dietary turmeric in ICR mice to determine the effects of turmeric when used as a dietary chemoprevention agent. After dietary turmeric was given for three or six weeks to ICR male mice, the immune responses were characterized. The methods of characterization involved; the determination of the T cell subpopulation in the spleen, relative mRNA expression levels of IFN-γ and TNF-α and serum lysozyme activity. Dietary turmeric was found to decrease spleen weight, decrease the proportion of CD4-CD8+ T cells, and decrease phagocytic activity. These results suggest that turmeric might alleviate abnormal chronic inflammation by the action of immune suppression.