Soybean lecithin liposomes composed phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidic acid were prepared by using the previously developed supercritical reverse phase evaporation method. The effect of phospholipid composition on the formation of liposomes and physicochemical properties were examined by means of trapping efficiency measurements, transmission electron microscopy, dynamic light scattering and zeta potential measurements. The trapping efficiency of liposomes for D-(+)-glucose made of CNA-Ⅰ which contains approximately 95% phosphatidyl choline is higher than that of CNA-II and CNA-O which contain approximately 32% phosphatidyl choline. However there is no any difference between the trapping efficiency of liposomes for D-(+)-glucose made of CNA-II which has saturated hydrocarbons tails and that of liposomes made of CNA-O which has unsaturated hydrocarbon chains. The electron micrographs of liposomes made of CNA-II and CNA-O show small spherical liposomes with diameter of 0.1~0.25μm, while that of CNA-I shows large unilamellar liposomes with diameter of 0.2~1.2μm. These results clearly show that phospholipid structure of phosphatidylcholine allows an efficient preparation of large unilamellar liposomes and a high trapping efficiency for water soluble substances. Liposomes made of CNA-II and CNA-O remained well-dispersed for at least 14 days, while liposome suspension made of CNA-I separated in two phase at 14 days due to aggregation and fusion of liposomes. The dispersibility of liposomes made of CNA-I is lower than that of CNA-II and CNA-O due to the smallar zeta potential of CNA-I.

본 연구는 daminozide의 엽면 살포방법를 이용하여 국내육성 스프레이 국화 ‘일월(Ilweol)’의 하계 고온에 의 한 꽃목 길이의 과도한 신장을 억제함으로서 품질을 향상 시키기 위해 수행하였다. 처리 시기는 화뢰 발달과정별로 3단계(stage I, II, III)로 나누고 daminozide의 처리농도는 0, 500, 1000, 2000mg·L−1로 구분하여 조합 처리하였다. 절화장과 꽃목 길이는 각각 stage III와 stage II에서 줄기 신장 억제효과가 가장 크게 나타났고, daminozide의 농도 가 증가할수록 절화장과 꽃목 길이는 감소하였다. 화폭은 무처리구에 비하여 처리시기가 늦어질수록 증가하였으 며, daminozide의 농도가 증가할수록 비례적으로 감소 하였다. 선단부 화방의 배열각도는 시기별로는 stage III 에서 농도에서는 2,000mg·L−1 daminozide 처리에서 각각 가장 크게 나타났다. 전체 처리 중에서는 2,000mg·L−1 daminozide를 stage II에 처리시 무처리구 대비 31.3%가 증가하여 가장 높은 배열각도를 보여주었다. 화수와 총 화뢰수도 2,000 mg·L−1 처리시 가장 많았으며, 처리시 기별로는 stage I에서 가장 많았다. 국내육성 스프레이 품종 ‘일월(Ilweol)’의 하계 재배시 꽃목(소화경)의 신장을 가장 효과적으로 억제하고 화서배열을 고르게 하며 화수 증가를 유도하는 daminozide의 처리농도와 시기는 2,000mg·L−1와 Stage III로 나타났다. 그러나 이 처리는 절화장과 화폭의 감소가 크게 나타나 상품성의 또 다른 면에서 불리하게 작용하였다. 따라서 실용적인 daminozide 의 처리시기와 농도는 절화의 품질을 감안할 때 Stage III 에서 500 mg·L−1로 판단되었다.

The genes encoding cellulases, which belong to glycosyl hydrolase families have been cloned from the basidiomycetous mushrooms. The transcripts of cellulase genes are strongly induced when the mycelia are grown in medium containing crystalline cellulose, and they are not expressed in medium containing glucose, but how insoluble substrates such as microcrystalline cellulose are recognized by these fungal cells is not clear. The polypore mushroom Polyporus arcularius is a wood-decomposing basidiomycete that produces at least three types (I, II, and IIIa) of carboxymethyl cellulase (CMCase) when the medium contains crystalline cellulose as the sole carbon source and produced mainly cellobiose in the medium. The genomic and cDNA clones encoding the family 12 endoglucanase (CMCase IIIa) gene (cel3A) of P. arcularius have been sequenced, and Cel3A has been expressed as an active enzyme in Escherichia coli. To determine the role and function of each type of cellulase in the degradation of crystalline cellulose by basidiomycetous mushrooms, the structure of all of the cellulase genes should be investigated, but the nucleotide sequences of the other cellulase genes in P. arcularius have not yet been reported. In the current study, the genomic and cDNA clones encoding the endoglucanases (cel4), and the two cellobiohydrolases (cel1 and cel2) of P. arcularius sequenced and characterized. The predicted amino acid sequence of Cel1 Cel2, Cel3a and Cel4 are similar to glycosyl hydrolase family 7, 6 12 and 5 protein, respectively. The expressions of the all cellulase genes (cel1 cel2, cel3a and cel4) were induced by Avicel (microcrystalline cellulose) and cellopentaose but repressed by glucose, cellobiose, cellotriose, and cellotetraose. There was a low level of transcription of both genes regardless of the carbon source. These results suggest that P. arcularius cells constitutively express a very low level of cellulase that can degrade insoluble crystalline cellulose and that the transcription of celluases in the cells is induced by products produced by these endoglucanases such as cellooligosaccharides. From our findings, we propose a possible mechanism for the recognition and degradation of insoluble crystalline cellulose by fungal cells.

무포자형성 자연돌연변이 균주의 선발 및 특성검정 결과 ASI 2069 균주가 무포자이면서 수량성이 높으나 상품적 가치가 없어 원형질체 재생에 의한 단핵화로 Nh36 (neohaplont 36) 등 4균주를 분리하였다. 원형1호(ASI 2180) 및 무포자느타리간의 단핵교배 (Mon-Mon)를 시도한 결과 128교배조합수를 얻어 이 중 30균주를 특성 검정한 다음 자실체형성 및 포자비산량 조사에 의해 소포자형성 유망 13균주를 선발하였다. 무포자균주의 스크리닝법을 개발하기위해 포자형성 및 감수분열에 관여하는 helicase, DMC1(recombinase) 및 Spo11(topoisomerase II)유전자를 이용하여 PCR 증폭한 결과 단핵화균주 Nh36 및 교배체 2균주 중에서 Spo11유전자가 증폭되지않아 무포자 균주 스크리닝 방법으로 가능하리라 사료된다.

The objective of this study was to evaluate the effects of co-culture of bovine oocytes with cumulus cells on in vitro maturation and development following in vitro fertilization in bovine oocytes. Bovine cumulus-oocyte complexes (COCs) and denuded oocytes (DO) were co-cultured with the cumulus cells in TCM199 for 20~22 hr, and evaluated the nuclear type of oocyte. After in vitro maturation, oocytes were coincubated for in vitro fertilization with frozen-thawed spermatozoa selected by 65% percoll in DM-Heparin and DM-Caffeine for 15~18 hr. Presumptive zygotes were cultured for 48 hr in CR1aa in vitro culture medium with 10% FBS, and evaluated the cleavage rates. The results confirmed that the highest percentage of metaphase II (M-II) stage was observed in COCs (30.1±3.5%, 24.2±1.8%) as compared to DO (7.1±1.3%, 17.4±13.9%) (p<0.05). In addition, the increased cleavage rates were obtained from COCs (69.6±2.1%, 75.6±2.9%) when compared to DO (21.6±7.5%, 29.5±12.6%) (p<0.05). In conclusion, this study suggested that cumulus cells secreted positive factors during in vitro maturation of oocytes and early embryonic development after in vitro fertilization of bovine oocytes.

This study is a substantive, qualitative survey targeting 500 high school students to discern the television and Internet media viewing behavior of secondary schools in a populous area such as Niigata City and Nagaoka City where presumably provides different sociological backgrounds and levels . The purposes of the study are a) to basically discern the major media viewing behavior of students, b) to examine the significance of the Cellular phone novels (KEITAI novels) in education from the perspective of media literacy based on the examining the effectiveness of KEITAI novels as teaching materials for educational guidelines. The survey results showed that students used the Internet mainly through their cellular phones. For both television and Internet viewing, the study showed a couple of unintentional tendencies: i) students avoid talking with friends about viewing their favorite media, ii) students wrap themselves in their media viewing depending on the situation and place. The usage of cellular phones in Japanese high schools seems ubiquitous. Students prefer reading using cellular phones. Cellular phone novels (Keitai novels) are widely read in Japan.

목적 : 본 연구는 노인 관절염환자를 대상으로 일상생활수행능력에 따른 방문재활서비스에 대한 요구도를 파악하 여 노인 관절염환자에 대한 방문재활치료모델개발의 기초자료로 활용하기 위한 것이다. 연구방법 : 2009년 8월 현재 대전광역시에 위치한 3개의 노인복지관과 1개의 주간보호센터에 등록된 재가 관절염 환자를 대상으로 설문을 실시하였다. 설문지는 총 150부를 배부하여 110부가 회수되었고, 회수된 설문지 중 79부 (72%)가 분석에 사용되었다. 설문지는 방문재활서비스 요구도(10문항)와 관절염상태를 평가하기 위한 한국형 WOMAC(Western Ontario and McMaster University Osteoarthritis)검사와 수정 바텔지수(Modified Barthel Index: MBI)로 구성하였다. 한국형 WOMAC과 수정바텔지수(MBI), 시각적통증검사(Visual Analogue Scale: VAS)의 상관성을 알아보기 위해 피어슨 상관분석을 실시하였고, 한국형 WOMAC의 기능 수준에 따른 일반적 사 항, 의학적 사항, 방문재활서비스 인식도, 방문재활서비스의 필요성과 이유, 방문재활서비스의 구체적 욕구에 대하 여 교차분석(3XR)을 통해 분석하였다. 유의수준 α는 .05로 하였다. 결과 : 대상자 79명의 진단명은 퇴행성관절염 환자가 61명(79.2%)이었으며, 통증이 가장 많이 발생하는 부위는 슬 관절로 나타났다. 방문재활서비스에 대한 인식도는 낮았지만 응답자의 93.7%(74명)가 필요하다고 응답하였고, 기능별로는 1등급에서는 15명(19.0%), 2등급에서는 44명(55.7%), 3등급에서는 15명(19.0%)이 방문재활서비 스가 필요하다고 하였다. 결론 : 본 연구의 결과에서 재가 노인 관절염 환자는 가정에서 제공되는 재활서비스에 대해 높은 요구도를 갖고 있 음을 알 수 있다. 향후, 재가 관절염 노인들을 위한 실증적인 방문재활서비스에 대한 연구가 지속적으로 보완되어 야 할 것이다.

전라남도 보성의 북부산지를 대표하는 천봉산(608m)일대에 자생하는 관속식물들을 밝히고 주요 식물들을 파악하였다. 2005년 3월부터 2005년 10월까지 총 4차례 현지조사를 통해 밝혀진 소산식물은 104과 307속 446종 3아종 56변종 6품종의 511종류로 조사되었다. 이 중 한반도 특산식물은 총 12분류군이 확인되었으며, 산림청 지정 한국 희귀식물은 8종류가 조사되었다. 환경부 지정 특정 식물종은 V등급 2종류, IV등급 1종류, III등급 8종류, II등급 7종류, I등급에는 28종류 등 총 46종류가 분포하고 있음을 알 수 있었다. 귀화식물은 총 38분류군, 귀화율은 7.4%로 전남지역의 평균 귀화식물분포보다 높게 나타났다.

정도리 해안방풍림은 완도 정남지역으로 바다와 육지 사이에 위치하면서 여름철 강한 남동풍을 막아 줌으로서 육상 경작지나 어촌 마을을 보호하는 육상생물이 서식하는 생태적 천이지역으로서 매우 중요한 곳이다. 정도리 방풍림의 식물상은 93과 250속 335종 42변종 7품종으로 총 384분류군이다. 정도리지역은 식물구계학적으로 남해안아구에 속하고 멸종위기 야생동 식물II급 종인 대흥란이 확인되었다. 희귀식물은 대흥란, 두루미천남성, 말나리, 모감주나무, 수정난풀 및 백량금으로 6분류군, CITES 보호종인 보춘화와 대흥란 2분류군, IUCN 보호종인 생달나무 1종이 분포하는 것으로 확인하였다. 그리고 특산식물은 새포아풀, 죽대, 옥녀꽃대, 소사나무 및 털괴불나무 등 7분류군이 분포하는 것으로 확인하였다. 본 조사지역 귀화식물은 11과 23속 27종 5변종으로 총 32분류군이 조사되어 귀화율은 8.33%이었으며 도시화지수는 11.81%이었다.

The purpose of this study is to investigate the effects of closed and open kinetic chain exercise for increasing knee joint function on the knee position sense in the normal adults. Thirty normal adults(male 15, female 15; mean age: 22.13±2.58 years) were participated in this study into two groups, each with 15 people. The group I was trained that closed kinetic chain exercise on the knee joint and the group II was trained that open kinetic chain exercise on the knee joint. Exercise programs performed for 4 weeks, 3 times a week were using Shuttle 2000-1 closed kinetic chain exercise and Knee Extensor open kinetic chain exercise(HUR, Filand). The results of this study were as follows: 1) There were statistically significant decreasing of measuring error degree in 0-20。were found between before and after training in closed kinetic chain exercise(p<.05). 2) There were statistically significant decreasing of measuring error degree in 21-40。were found between before and after training in closed kinetic chain exercise(p<.05). 3) There were statistically significant decreasing of measuring error degree in 41-60。were found between before and after training in closed kinetic chain exercise(p<.05). 4) There were statistically significant decreasing of measuring error degree in 0- 20。were found between before and after training in open kinetic chain exercise(p<.05). 5) There were statistically significant decreasing of measuring error degree in 21-40。were found between before and after training in open kinetic chain exercise(p<.05). 6) There were statistically significant decreasing of measuring error degree in 41- 60。were found between before and after training in open kinetic chain exercise(p<.05). In conclusion, these results suggest that closed and open kinetic chain exercise has increased in the knee joint proprioception between before and after training. Especially, closed kinetic chain exercise could be more useful intervention than open kinetic chain exercise for increasing proprioceptive sense.

The purpose of this paper is to investigate into the meaning of the New Brutalism sensed in the late church buildings of the Swedish architect Sigurd Lewerentz (1885-1975). St Mark's (1956-64) and St Peter's (1962-66) churches, his grand finale designs, have a unique architectural vocabulary of rough brickwork. The brick treatment is the basis on which Reyner Banham (1966) discussed him concerning the New Brutalism, and the point that this research focuses on. This paper explores the brutalist character of the buildings from two aspects - interpretational level of individual buildings and historical level of a broader view. First, the character of two churches could be interpreted with the phrase of 'play between brutality and sacredness'. The rough surface of brick and mortar in the buildings symbolises brutality and the vault of their chapels' ceiling sacredness. And the two characteristics meet and play on the rough vault surface. Second, in the historical point of view, this paper argues that the buildings made a giant leap for Swedish modern architecture, which had been at a deadlock owing to the compromising attitude of the New Empiricism since 1940s. And the Swedish New Empiricism (or the New Humanism), spread to Britain as "Welfare State architecture" after World War II, brought about reaction of the young British architects such as the Smithsons and became the background that made the new brutalist mood. However, considering that the term of the New Brutalism was first used in Sweden by Hans Asplund, Lewerentz's brutalist late churches - which seemingly had nothing to do with the British nor the Corbusian lineage - are also meaningful in that they confirmed the tendency in its homeland. In conclusion, this paper argues that St Mark's and St Peter's churches with the brutalist characteristics should be regarded as crucial buildings not only in Lewerentz's personal career but also in Swedish and international architectural history.

Mamestra brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) was isolated from naturally infected Mamestra brassicae (Lepidoptera: Noctuidae) larvae in Korea. Restriction endonuclease fragment analysis using EcoRI, PstI, and BamHI estimated that the total genome size of MabrNPV-K1 is about 150 Kb. The full genome sequences of MabrNPV-K1 were determined, analyzed and compared to those of other baculoviruses. The MabrNPV-K1 genome consisted of 152,471 bp and had an overall G + C contents of 39.90 %. Computer-assisted analysis predicted 159 open reading frames (ORFs) of 150 nucleotides or greater that showed minimal overlap. The gene content and arrangement in MabrNPV-K1 were most similar to those of Mamestra configurata nucleopolyhedrovirus-B (MacoNPV-B), including three polh, p10 and lef-8 gene homologues. The MabrNPV-K1 genome contains four homologous repeat regions (hr1,hr2,hr3,hr4) that account for 3.1% of the genome. The genomic positions of MabrNPV-K1 regions hr1– hr4 are conserved with the genomic positions of MacoNPV-B hr1–hr4. This indicates that the position of MabrNPV–K1 hrs is conserved with regard to both the upstream and downstream genes. Given that hrs share higher similarity within a virus strain than any hrs between species, this evidence further indicates that hrs play a fundamental role in viral life cycle and replication process appears to be tightly linked to functional conservation. The dot plot analysis, percent identity of the gene homologues and a phylogenetic analysis suggested that MabrNPV-K1 is a Group II NPV that is closely related to MacoNPV but with a distinct genomic organization.

To fabricate TiO2 nanoparticle-based dye sensitized solar cells (DSSCs) at a low-temperature, DSSCs were fabricated using hydropolymer and ZnO nanoparticles composites for the electron transport layer around a low-temperature (200˚C). ZnO nanoparticle with 20 nm and 60 nm diameter were used and Pt was deposited as a counter electrode on ITO/glass using an RF magnetron sputtering. We investigate the effect of ZnO nanoparticle concentration in hydropolymer and ZnO nanoparticle solution on the photoconversion performance of the low temperature fabricated (200˚C) DSSCs. Using cis-bis(isothiocyanato)bis(2,20 bipyridy1-4,40 dicarboxylato) ruthenium (II) bis-tetrabutylammonium (N719) dye as a sensitizer, the corresponding device performance and photo-physical characteristics are investigated through conventional physical characterization techniques. The effect of thickness of the ZnO photoelectrode and the morphology of the ZnO nanoparticles with the variations of hydropolymer to ZnO ratio on the photoconversion performance are also investigated. The morphology of the ZnO layer after sintering was examined using a field emission scanning electron microscope (FE-SEM). 60 nm ZnO nanoparticle DSSCs showed an incident photon-to-current conversion efficiency (IPCE) value of about 7% higher than that of 20 nm ZnO nanoparticle DSSCs. The maximum parameters of the short circuit current density (Jsc), the open circuit potential (Voc), fill factor (ff), and efficiency (η) in the 60 nm ZnO nanoparticle-based DSSC devices were 4.93 mA/cm2, 0.56V, 0.40, and 1.12%, respectively.

Al2O3 has received wide attention with established use as a catalyst and growing application in structural or functional ceramic materials. On the other hand, the boehmite (AlO(OH)) obtained by sol-gel process has exhibited a decrease in surface area during phase transformation due to a decline in surface active site at high temperature. In this work, Al2O3-CuO/ZnO (ACZ) and Al2O3-CuO/CeO (ACC) composite materials were synthesized with aluminum isopropoxide, copper (II) nitrate hemi (pentahydrate), and cerium (III) nitrate hexahydrate or zinc (II) nitrate hexahydrate. Moreover, the Span 80 as the template block copolymer was added to the ACZ/ACC composition to make nano size particles and to keep increasing the surface area. The ACZ/ACC synthesized powders were characterized by Thermogravimetry-Differential Thermal analysis (TG/DTA), X-ray Diffractometer (XRD), Field-Emmision Scanning Electron Microscope (FE-SEM), Bruner-Emmett-Teller (BET) surface analysis and thermal electrical conductivity (ZEM-2:M8/L). An enhancement of surface area with the addition to Span 80 surfactant was observed in the ACZ powders from 105 m2/g to 142 m2/g, and the ACC powders from 103 m2/g to 140 m2/g, respectively.

The bioactive effects of ethanol extracts from fly maggot (ME) on reduction of plasma lipids levels in rats fed high-fat diets (Expt. Ⅰ), and on liver function recovery of hepatotoxicity rats by intraperitoneal injection of carbon tetrachloride (CCl4) or by orally administration of alcohol (Expt. II) were investigated. In expt. I, twenty seven, male rat SDS(sprague dawley strain) were randomly assigned to three treated groups, including normal control group, HF (group with high fat diets which have no extracts) and HFE (HF plus orally administered doses of ME extract at 5.0 mg/100g of body weight). In expt. II, forty five, male rats (SDS) were randomly assigned to each of the five groups: T1 (control), T2 (intraperitoneal injection of CCl4), T3 (intraperitoneal injection of CCl4 after orally administered with ME), T4 (orally administered with combination of ME and alcohol), T5 (orally administration of ME after orally administered with alcohol). There were significant decreases in plasma (TAG), (TC), (LDL-C) in the HFE group with orally administered doses of ME at 5.0 mg/100g of body weight, respectively, however, the (HDL-C) were significantly increased in HFE group as compared to HF group with high fat diets which have no extracts (p〈0.05). The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transferse(γ-GTP) and bilirubin were highest in T2 or T3, and high in order T4 or T5, and lowest in T1 except for bilirubin which has same with T4, T5 (p〈0.05). The high recovery of liver damage by CCl4 from the light microscopic appearance was observed in rats (T3) with extracts, and also high in T4 than T5 by orally administrated with alcohol. In conclusion, the ethanol extracts from fly maggot may have a bioactive effects to prevent for human lipids disorder and alcoholic disease.

The study investigates the various Acceptance Control Charts (ACCs) based on the factors that include process independence, data weighting scheme, subgrouping, and use of control charts. USL - LSL 〉 6Σ that used in the good condition processes in the ACCs are designed by considering user's perspective, producer's perspective and both perspectives. ACCs developed from the research is efficiently applied by using the simple control limit unified with APL (Acceptable Process Level), RLP (Rejectable Process Level), Type I Error α, and Type II Error β. Sampling interval of subgroup examines i.i.d. (Identically and Independent Distributed) or auto-correlated processes. Three types of weight schemes according to the reliability of data include Shewhart, Moving Average(MA) and Exponentially Weighted Moving Average (EWMA) which are considered when designing ACCs. Two types of control charts by the purpose of improvement are also presented. Overall, α, β and APL for nonconforming proportion and RPL of claim proportion can be designed by practioners who emphasize productivity and claim defense cost.

본 시험은 청보리, 밀, 호밀, 트리티케일, 귀리 등 총체맥류 주요 품종에 대하여 봄 파종시 출수 여부, 생육, 수량 및 사료가치 등을 적기 파종과 비교하고자 2008/2009년에 충남농업기술원(예산) 밭 사료포에서 수행되었다. 시험초종 및 품종은 청보리(영양, 파성 I), 밀(금강, 파성 II), 호밀(곡우, 파성 III 추정), 트리티케일(신영, 파성 III 추정), 귀리(삼한, 파성 II 추정)이었고, 파종기는 가을 적기파종(10월 18일)과 봄