We investigated eight active natural antimicrobials for preservation of functional beverages that are usually degraded by yeasts rather than by bacteria due to a high sugar content and a low pH. Five strains of yeasts (S. cerevisiae, Z. bailii, P. membranaefaciens, C. albicans, and P. Anomala) were tested with eight natural antimicrobial agents (ε-polylysine, yucca extract, vitamin B1 derivative, scutellaria baicalensis extract, chitooligosaccharid, allyl isothiocyanate, sucrose-fatty acid ester, and oligosaccharide). The lowest minimal inhibitory concentrations (MIC) were 10 ppm for oligosaccharide and sucrose-fatty acid ester against S. cerevisiae and Z. bailii, 10 ppm for allyl isothiocynate against P. membranaefaciens and C. albican, and 10 ppm for allyl isothiocynate and oligosaccharide against P. anomala . No growth were observed for five kinds of yeasts in functional beverages containing sodium benzonate at concentration of 0.015% or higher. The resistance of S. cerevisiae, Z. bailii, and P. Anomala against natural antimicrobial agents was lower than those of P. membranaefaciens and C. albican. Allyl isothiocyanate, oligosaccharide, and sucrose- fatty acid ester showed the highest antimicrobial activities among the eight tested antimicrobials. These results can be applied to develop new natural antimicrobial agents to improve microbial quality of functional beverages.

Antifungal activity of seven natural antimicrobials, such as ε-polylysine, extract of Yucca shidigera, TLS (vitamin B1 derivative), BMB-FS, chitooligosaccharide, KDSP 001, and KDSP 002 were investigated for their applications in functional beverage. Five fungi including Aspergillus niger, Penicillium citrinum, Rhizopus oryzae, Fusarium moniliforme, and Mucor rouxii were applied as test fungi and mininum inhibitory concentrations (MICs) of antimicrobials were examined. TLS exhibited the strongst antifungal activity among tested antimicrobials and the growth of all fungi was inibited at 100 ppm. The antifungal activity of BMB-FS appeared different. The growth of Fusarium moniliforme was inhibited by BMB-FS at 100 ppm, but it exhibit antifungal activity on P. citrinum, and M. rouxii at 1000ppm. MICs of TLS appeared to be 60 ppm for four test fungi except A. niger (100 ppm). These results indicate the possible usage of TLS and BMB-FS as natural antimicrobials in functional beverage.

The objective of this study was to examine the effect of eCG and various concentrations (20, 40, and 80 ) of porcine FSH on nuclear maturation and intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were matured in TCM-199 supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones (10 IU/ml hCG and 10 IU/ml eCG or FSH) for the first 22 h and then further cultured in hormone-tree medium for an additional 22 h. Nuclear maturation of oocytes () was not influencem foreCG and various concentrations FSH. Embryonic development to the cleavage stage () and mean number of cells in blastocyst ( cells) after PA were not altered but blastocyst formation e-treignificaddlor(p<0.05) improvem forthe supplementation eith 80 FSHr(64%) compared to 47%, io8%, iand 47% in oocytes that were treated with eCG, 20,i and 40 FSH,i numectivelo. In SCNT, fusion () of cell-cytoplast couplets and siosequent embryo cleavage () were not influencem fordifferent gonadotropins but blastocyst formation tended to increase forthe supplementation eith 80 FSHr(25% vs. ). Our nuults demonstrated that oocyte maturation and embryonic development after PA and SCNT e-frinfluencem fortype of gcem fortype of gits concentration. In this study, supplementation of maturation medium eith 80 FSHrimproved preimplantation development of PA and SCNT pig embryos, probably by increasing intracellular GSH concentration of matured oocytes.

The purpose of this study was to investigate the antimicrobial activities of natural antimicrobials (10 formulas, F1~F10) against yeasts in functional beverages. The growth rates of yeasts were different with the ten different natural antimicrobial formulas tested. Yeasts grew for 14 days and the antimicrobial effect was observed between 14 and 18 days. Levels of S. cerevisiae, Z. bailii, and P. membranaefaciens were reduced to the limit of detection (ND) < 10 CFU/mL) after 28 days. Resistance against the antimicrobial effect was greatest for P. membranaefaciens, which grew to a level of 0.12~1.48 log10 CFU/mL after 14 days and was reduced to a level of 1.61~3.55 log10 CFU/mL after 28 days. The resistance of C. albicans was also high with a growth level of 0.13~1.28 log10 CFU/mL after 14 days and reduction to 1.51~5.30 log10 CFU/mL after 28 days. The antimicrobial effect of F10 was strongest for P. membranaefaciens. Every treatment reduced the microbial levels to 2.68~5.62 log10 CFU/mL after 6 months. F2, F4, F5, F6, and F10 reduced the C. albicans level to ND after 28 days while F1, F3, F8, and F9 reduced yeasts to the ND level after 6 months. The antimicrobial activities observed here will be useful for development of natural antimicrobials.

Polymer gel implant for soft tissue augment is demanded biological safety properties. This study is designed process and equipment producing for obtain micro bead using the hyaluronic acid water solution. This equipment is composed of cooling pipe, air pump, dispenser and process is consecutive/simple for preservation from environmental contamination. Besides, without difficulty remove the residual agent after crosslink. We evaluate to in vitro cytotoxicity test for verification of hyaluronic acid gel obtained by this equipment and process. This product is "non cytotoxcity" from the result of evaluation cytotoxcity test.

The Automobiles with a combustion engine use a transmission for increasing drive force, reverse-move and maintaining non-load condition in general. In the Electric automobile system, output-axial can be operated just by controlling the motor manipulated with power. It also does not need reverse-gear by rotating the motor in the opposite direction. Designed to assemble two different types of planetary gear ratio and to be input torque of input-axial in sun gear and divide ring gear Ⅰ, Ⅱ and then after one of ring gear is fixed, in accordance with the planetary gear velocity ratio, the first gear has 0.136 decreased speed and second gear has 0.240 decreased speed, separately. While the planetary gear type transmission showed over the 92% of efficiency in the all of velocity range. The transmission proposed in this study can be expected to apply to the electric vehicle or others.

젖소의 분만 전․후기는 일반적으로 전환기라고 칭하며 분만후 유생산을 준비하기 위해 동물체내 대사 및 생리적 상태가 급격히 변화하는 시기라 할 수 있다. 젖소는 이 시기에 간조직에서 당신합성을 통해 유당합성을 위한 glucose의 생산을 촉진하고, 지방조직에서는 분만 전부터 지질을 축적하고 비유개시에 맞추어 혈중 NEFA (nonesterified fatty acid)의 농도를 증가시켜 유지방 합성을 준비한다. 이러한 대사조절에 epinephrine 및 insulin이 조절 호르몬으로 작용하여 유생산을 위한 탄수화물 및 지질대사를 변화시키고 사료 섭취량의 부족에 기인한 전체 에너지의 감소를 체내 영양소의 재분배로 충족시킨다.

The principal objective of this study was to develop and standardize a preparation method for low-sodium tomato jangachi (traditional Korean pickle) via short-term fermentation with immature green cherry tomatoes. In order to determine the preferred concentrations of soy sauce and soaking temperatures of immature cherry tomato pickles in different stages of storage, we conducted an analysis of physico-chemical characteristics and microbiological properties, and also performed a preference test on samples of immature green cherry tomato pickles. Immature cherry tomatoes were prepared in three different soy sauce concentrations --20, 40, and 60%-- and three different soaking temperatures --60, 80, and 100℃-- and then stored for 28 days at 5℃. As a result, the pH increased significantly with increases in the amount of soy in the dipping solution (p〈0.05). The saltiness was maintained at levels of approximately 0.17~0.28% (20% group), 0.32~0.67%% (40%group), 0.48~1.00% (60% group) during storage periods. These results show that the saltiness of immature cherry tomato pickles was substantially lower than that of commercial pickles. The contents of reducing sugar and lightness decreased significantly with increasing concentrations of soy dipping solution. The redness and yellowness values of the tomatoes decreased significantly with increasing concentrations of soy sauce. Additionally, the lower the concentration of soy sauce used, the more rapidly the hardness of the immature cherry tomato pickles was reduced at 100℃. PME activity moved within a narrow range, and then stabilized during the storage period. With regard to the results of the consumer preference test, 20%-100℃ was the most preferred condition overall, 40%-80℃ was the condition in which the texture was most preferred, and 40%-80℃ was the condition that yielded the highest color scores.

For the environment-friendly control of beet armyworm, Spodoptera exigua, in spring onion fields, we have examined an alternative application method. Twenty-five insecticides registered for spring onion were tested for control effect against the beet armyworm in the laboratory, then the best 9 chemical and a single biological insecticides were selected and compared with 2 new isolates of Bacillus thuringiensis in a polyvinyl house. After that, 4 chemical and 3 B. thuringiensis treatments were used in the field for the control of beet armyworm in the spring onion. Two application methods are used: one is triple treatments with the same chemical and the other is alternative application with different chemicals and B. thuringiensis for 7 days intervals. Indoxacarb WP – chlorfluazuron EC – B.t. var. kurstaki CAB141 and indoxacarb WP – methoxyfenozide + spinosad SC – Chlorfluazuron EC – B.t. var. aizawai CAB109, B.t. var. kurstaki CAB141 showed greater than 78% mortality of beet armyworm larvae and greater than 43% damage decrease in spring onions infested by beet armyworm. These results showed that alternative applications had higher control effect than any other applications. It was suggested that alternative applications with microbial biological agents such as B. thuringiensis might minimize the development of insecticide resistance and might be used as the environment-friendly control of the beet armyworm.

The reduction rate of pesticide residues on spinach(bifenthrin, metalaxyl, procymidone), chard(bifenthrin, imidacloprid) and mallow(bifenthrin, chlorpyrifos, imidacloprid) were tested on each step of washing and boiling(spinach: 1, 3, 5min., chard: 3, 6, 9min., mallow: 10, 20, 30min.). The reduction rates of bifenthrin and procymidone by washing were 58~64% and 82%, and these were not changed significantly after boiling. In case of imidacloprid, the rates showed 43% on chard and 12% on mallow by washing, and these were highly increased to 94% after boiling. And the reduction rate of metalaxyl and chloropyrifos were 69% and 11% by washing, and 96~98% and 77~79% by boiling. Specifically we monitored the pesticide residues on both boiled vegetable and its water because there are used to cook as soup in Korea. The total residual amounts of imidacloprid and chloropyrifos were effectively removed on both boiled mallow and its water (12% → 34~40%, 11% → 76~79%), however, the other tested pesticides were not changed on pesticide residues when calculated with total amounts on boiled vegetable and its water. These explained the other pesticides were just moved vegetable to water by boiling.

All organisms are being exposed to harmful factors present in the environmental. The combined action of various factors is a distinguishing feature of modern life. An interaction between two chemicals is considered as synergistic when the effect produced

본 연구에서는 소비자 지향적 마케팅시대에 직면하여 국내외 농산물 소비수요의 변화에 대응하고 친환경농산물의 유통개선을 도모하기 위하여 소비자들의 친환경농산물인증제도 및 정책에 대한 인식을 조사분석 하였다. 조사연구 결과에 의하면 첫째, 친환경농산물의 소비저변을 확대시키기 위한 가장 시급한 문제는 생산자인 농민들이 아니라 소비자들에 대한 신뢰성을 확보하는 것이 무엇보다도 중요함을 알 수 있다. 이를 위해서는 다양하고 안전한 판로개척과 품질경쟁력 제고, 소포장 규격화 판매, 품질인증제도를 통한 브랜드화 추진, 리콜제 실시 등을 통해 소비자들로부터 신뢰를 얻어야 한다. 둘째, 정부의 친환경농산물정책에 대한 지속적이 홍보와 교육이 이루어지고 또한 감독강화가 이루어져야 할 것으로 생각된다. 특히 친환경농산물 유통구조에서 농민들과 직접 거래를 하는 직거래나 인터넷 거래를 활성화시킴으로서 소비자들에 의한 신뢰도를 높일 수 있는 계기가 마련될 것이다. 셋째, 친환경농산물에 대한 품질관리가 지속적으로 이루어져야 한다. 이는 중앙정부의 감독관리도 중요하지만 지역별 품질인증기관을 육성하고 품질인증기관이 생산과정에 대한 투명성을 철저히 보장할 수 있도록 제도화하여 친환경농산물에 대해 소비자들로부터 신뢰를 얻어야 할 것으로 보인다. 마지막으로 친환경농산물 생산농가가 판매에 대한 어려움을 지자체 등이 적극적으로 지원할 필요가 있을 것으로 판단된다.

본 논문은 움직이는 객체를 지속적으로 감시관찰하는 CCD 카메라의 자동 제어를 위한 신호 생성 알고리즘을 제시하고 있다. 제안된 알고리즘은 검출된 객체 위치와 영상 중심 사이의 수평 수직 변위들을 계산하고 변위들을 각으로 변환한다. 최종적으로 팬/틸트 신호가 변위각으로부터 생성된다. 제안된 알고리즘의 성능 평가를 위해 자동 제어에서 생성된 데이터와 수동 제어에서 측정된 데이터가 비교되고, 단순한 객체를 이용한 추적 실험이 수행되었다. 실험 결과는 두 데이터의 차이는 무시할 수 있을 정도이고, 팬/틸트 ±52o/±40o 영역에서 움직이는 객체가 ±13o/±10o 영역에 유지되는 것을 보여주고 있다.

This paper presents a novel single-step method to prepare the Ag nanometallic particle dispersed fluid (nanofluid) by electrical explosion of wire in liquid, deionized water (DI water). X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM) were used to investigate the characteristics of the Ag nanofluids. Zeta potential was also used to measure the dispersion properties of the as-prepared Ag nanofluid. Pure Ag phase was detected in the nanofluids using water. FE-SEM analysis shows that the size of the particles formed in DI water was about 88 nm and Zeta potential value was about -43.68 without any physical and chemical treatments. Thermal conductivity of the as-prepared Ag particle dispersed nanofluid shows much higher value than that of pure DI water.

The purpose of this study was to determine the reliability and validity of the fear-avoidance beliefs questionnaire (FABQ) in assessing Korean patients with low back pain. We performed translation and cross-cultural adaptation of the questionnaire and enrolled 52 patients (18 males and 34 females) with low back pain. The subjects completed a standardized self-administered questionnaire that included the FABQ assessment along with evaluations for the visual analogue scale (VAS), Oswestry disability index (ODI), self-rating anxiety scale (SAS), and Beck depression inventory-Korean version (K-BDI). The reliability of the obtained data was determined by evaluating the internal consistency on the basis of the intraclass correlation coefficient (ICC) and the Cronbach's alpha values, while the validity of the data was examined by correlating the FABQ scores to the VAS, ODI, SAS, and K-BDI scores. The translated versions of the FABQ showed good test-retest reliability: ICC (3, 1)=.90 (FABQ for physical activity) and .97 (FABQ for work) these values were statistically significant (p<.01). The Cronbach's alpha value for FABQ was .90 (p<.01). FABQ moderately correlated with pain, disability, anxiety, and depression (p<.01). The results of this study indicate that the Korean version of the FABQ is a reliable and valid instrument for measuring fear-avoidance beliefs in Korean patients with low back pain.