Earthquake is one of the hazard so hard to provide because it is difficult predicted occurred time, scale and characters. Neighbors which have damaged by earthquake look out for statement of damage, It is bigger than nation which has not prepared seismic code at ordinary time seismic damage which belong to loss of life. The earthquake in Kobe, Japan showed that the damages were concentrated on the buildings which were not considered to be protected from the earthquakes. Seismic code applied structures was small damaged but non applied structures was much damaged such as collapse and loss of life. In this study, apartment structures in Korea analyze about earthquake hazard and evaluate seismic performance. Through the this study we have notice of earthquake hazard for apartment structures which live a lot of population of Korea and suppose necessary for seismic retrofit.

In this study, the seismic performance of RC school buildings which were not designed according to earthquake-resistance design code were evaluated by using response spectrum and push-over analyses. From the results of analysis, the efficiency of the seismic retrofitting methods RC shear wall, steel frame, RC frame and PC wing wall for existing RC school buildings was evaluated and analysised. The analysis result indicate that the inter-story drift concentrated in the first floor and most plastic hinge forms in the column of the first story. And results of analysis of the efficiency of the seismic retrofitting indicate that inter-story drift significantly reduced and ductile behavior is expected.

It is not easy for porcine embryos produced by in vitro systems to develop into blastocysts with high quality. To solve this problem, many researchers have developed novel culture methods. However, the formation of blastocysts with high quality is still low. In this study, we aimed to produce piglet following transfer of in vitro produced early embryos ( cell stage embryos) or morula and blastocyst. The cell stage embryos were transferred to five estrus-synchronized recipients (200 embryos per recipient). One of the five sows farrowed three piglets, which contain two live piglets and one dead piglet, 114 days after embryo transfer. However, two recipients transferred with morula and blastocysts did not farrow. Microsatellite analysis confirmed that the genomic DNA of two live piglets were not genetically identical to that of the recipient. These results indicate that it is possible to obtain piglets by transfer of early embryos produced by in vitro production (IVP) systems.

부탄올을 투과증발 공정으로 분리하기 위하여 복합공정에 의하여 투과증발막을 제조하였다. 상업용 poly(dime-thylsiloxane) (PDMS) 막을 plasma 처리시키거나, polysulfone, poly(ether imide) 막을 지지체로 사용하여 plasma 처리 및 PDMS 코팅의 복합공정을 적용하였다. 헥산계열과 실란계열 유기 화합물을 사용하여 PDMS막을 plasma 처리하였을 경우 막 표면의 소수성을 증가시켜서 부탄을 선택도가 12.56까지 향상되었다. 반면에 투과량은 막 표면의 소수성 증대와 free volume의 변화로 인해 1.15 kg/m 2 ⋅hr까지 감소되어 선택도와 반대의 성향을 나타내었다. 막의 소수성이 증가함에 따라 접촉각과 상대적 sorption 비가 증가하였고, 부탄을 선택도도 향상되었다. PDMS 코팅 용액에서 prepolymer의 함량이 높을수록 부탄올 선택도가 증가하였다. PDMS 코팅과 plasma 처리 공정의 순서에 따른 영향을 조사하였다. 부탄올과 노르말 헥산으로 plasma 처리할 경우 plasma처리, PDMS 코팅 순으로 제조된 막의 분리 성능이 우수하였고 hexamethyldisilane과 hex-amethyldisilazane을 사용한 경우에는 역순으로 제조된 막의 분리 성능이 더 우수한 것으로 나타났다.

In order to improve the management of food waste treatment facility, this study investigated the emission characteristics of odorous compounds around treatment process and deodorization equipment of three food waste treatment facilities(the transformation, feed production and composting facility). Furthermore, the removal experiment of odorous compounds using various absorbent was conducted. The odor concentrations of food waste storage hopper and separation process were higher than other processes and the major odorous compounds were methyl mercaptan, acetaldehyde, hydrogen sulfide and ammonia. The odor removal efficiencies of deodorization equipment such as activated carbon tower, wet scrubber and biofilter were mostly insufficient. Especially, the removal efficiency of wet scrubber is lower than the others, therefore the improvement of optimal operating condition is required. As a result of removal experiment of various absorbent, the removal efficiency was over 98% in case of 1% H₂SO₄+K₂HPO₄ for ammonia, KMnO₄ for acetaldehyde and KMnO₄ and 5% NaOH+KH₂PO₄ for hydrogen sulfide and methyl mercaptan. To achieve the effective odor control of food waste treatment facility, it is necessary increasing the removal efficiency of scrubber by using optimal absorbent for target odorous compounds.

Epithelial-mesenchymal transition (EMT) can play an important role in carcinogenesis of oral squamous cell carcinoma (OSCC). EMT is characterized by morphological and phenotypical change of epithelial cells into mesenchymal cells, and transcriptional repressor of E-cadherin, Snail is critical for EMT. In order to investigate the role of Snail and E-cadherin in OSCC, we analyzed the immunohistochemical pattern of Snail and E-cadherin in 18 OSCCs. The expression of Snail in the OSCC was increased whereas the expression of E-cadherin in the OSCC was decreased in comparison with those of normal oral mucosa, showing reverse correlation. Especially, the fibroblasts near the islands of OSCC showed the positivity of Snail, suggesting the reactive fibroblasts to the EMT of epithelial tumor cells. In metastatic squamous cell carcinoma in cervical lymph node, the positivity of Snail of tumor cells was higher than that of primary OSCC. We concluded that the increased Snail expression and the decreased E-cadherin expression were involved in the progression, invasion and metastasis of OSCC.

본 연구는 홀스타인, 한국재래산양 및 한우유로부터 glycomacropeptide(GMP)를 분리하였으며, 각 GMP의 trypsin 가수분해물의 혈소판응집 억제 효과를 in vitro 상에서 알아보았다. 홀스타인, 한국재래산양 및 한우의 GMP는 분자량이 모두 약 20 KDa이었으며, sialic acid 함량은 각각 36.86±2.36, 37.98±1.27 및 31.19±1.87㎍/mg이었다. 또한 모든 개체의 GMP에서 tyrosine이 검출되었다. 홀스타인, 한국재래산양 및 한우 GMP의 trypsin 가수분해물에 의한 혈소판 응집 억제율은 반응 30초에 4.02, 5.51 및 12.77%로 각각 나타나 시간이 경과할수록 감소하는 경향을 보였다. 혈소판의 현미경 관찰 결과 가수분해물 첨가 후 혈소판 수가 증가하였으나, 첨가 후 30초가 경과한 시점부터 혈소판 수가 감소하기 시작하여 120초 후에는 관찰 할 수 없었다. 본 실험 결과 bovine 및 caprine GMP의 trypsin 가수분해물에서 혈소판 응집을 억제할 수 있는 small peptide가 있는 것으로 생각되며, 향후 이러한 연구는 심근경색증 및 뇌혈전증을 예방할 수 있는 생리활성 물질로 이용될 수 있을 것이라 생각된다.

In this study, we assessed the effects of dietary supplementation with Ecklonia cava on blood glucose, lipid metabolism, and renal oxidative stress in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into a normal rat group fed on a control diet and diabetic rats fed on a control diet or supplemented with powder (15% w/w) or water extract of Ecklonia cava (2.5% w/w). Diabetes was induced by a single injection of STZ (60 mg/kg, ip) in citrate buffer. The animals were fed ad libitum with the experimental diet and water for 5 weeks. Dietary supplementation of Ecklonia cava powder and water extract was shown to reduce blood glucose levels in the diabetic rats, and the water extract was more effective than the powder. Dietary supplementation with Ecklonia cava also reduced LDL cholesterol and increased HDL-cholesterol levels in the diabetic rats. Renal glutathione S-transferase activity was increased in the diabetic rats as compared to the normal rats, but reverted to near control values as the result of dietary supplementation with Ecklonia cava. These results show that Eklonia cava exerts an anti-diabetic effect by improving blood glucose concentrations, LDL/HDL-cholesterol ratios, and antioxidative effects on the kidney in diabetic rats.

Vapor phase polymerization of a conductive polymer on a SiO2 surface can offer an easy and convenient means to depositing pure and conductive polymer thin films. However, the vapor phase deposition is generally associated with very poor adhesion as well as difficulty when patterning the polymer thin film onto an oxide dielectric substrate. For a significant improvement of the patternability and adhesion of Poly(3-hexylthiophene) (P3HT) thin film to a SiO2 surface, the substrate was pre-patterned with n-octadecyltrichlorosilane (OTS) molecules using a μ-contact printing method. The negative patterns were then backfilled with each of three amino-functionalized silane self-assembled monolayers (SAMs) of (3-aminopropyl) trimethoxysilane (APS), N-(2-aminoethyl)-aminopropyltrimethoxysilane (EDA), and (3- trimethoxysilylpropyl)diethylenetriamine (DET). The quality and electrical properties of the patterned P3HT thin films were investigated with optical and atomic force microscopy and a four-point probe. The results exhibited excellent selective deposition and significantly improved adhesion of P3HT films to a SiO2 surface. In addition, the conductivity of polymeric thin films was relatively high (~13.51 S/cm).

본 논문에서는 식품 중 퀴놀론계(QNs) 합성항균제(marbofloxacin, norfloxacin, danofloxacin, ciprofloxacin, enrofloxacin, difloxacin, sarafloxacin, oxolinic acid 그리고 flumequine) 9종을 분석하기 위하여, 액-액 추출 과정을 거쳐서 형광검출기가 장착된 액체크로마토그래피를 이용하여 QNs를 효율적으로 동시분석하는 방법을 확립하였다. 컬럼은 Zorbax Eclipse XDB-C8(150 mm×4.6 mm, 5 μm), 이동상 용매는 200 mM ammonium acetate buffer (pH 4.5)와 ACN로 기울기 용리를 사용하였으며, 유속은 1.5 ml/min, 그리고, 주입량은 10 μl로 설정하여 분석하였다. 확립 된 분석조건으로, 표준검정 곡선은 10-500 μg/kg의 농도범위에서 상관계수가 0.9989 이상의 양호한 직선성을 나타내었으며, 회수율은 50, 100 그리고 500 μg/kg의 농도에서 89.6-106.5 %로, 향상된 추출효율을 나타내었다. 검출한계는 1-16 μg/kg이었고, 정량한계는 3-47 μg/kg이었으며, 일내(intra-day)와 일간(inter-day) 정밀도(CV%)는 0.2-15.0 %, 0.5-11.7 %이었다. 따라서, 확립된 분석방법은 광어 및 계란 중의 QNs을 효과적으로 분석하는데 이용될 수 있을 것으로 사료된다.

유비쿼터스 환경에서는 모든 사물이 소통할 수 있는 환경이기 때문에 사물과 사물 사이, 사물과 사람 사이, 사람과 사람 사이의 진정성을 확보할 수 있는 수단이 필요하며, 이를 위하여 종래의 단편적 서비스에 머무르고 있는 인증기능을 통합적으로 관리할 필요가 있다. 따라서 인증서비스는 인증에 필요한 인증대상, 등록(대행)기관, 인증서비스제공기관, 인증을 검증해주기 위한 검증기관, 인증서비스제공기관의 인증서비스를 평가하기 위한 제3의 기관이 요구된다. 나아가 이러한 인증서비스의 전 과정에 걸친 업무의 투명성, 진정성, 안정성 등에 대한 관리․감독 및 정책을 수립․수행하기 위한 관리감독기관의 설립이 필요하다. 한편 기기인증모델의 경우에 인증기관이 등록(대행)기관을 통하여 인증수단을 발급하거나 직접 인증수단을 발급할 경우에 어느 경우에나 허위 혹은 부정확한 인증수단이 발급됨으로 인하여 인증수단을 발급받은 자나 그와 법률행위를 함으로써 손해를 입은 경우에는 그에 대한 손해를 배상할 책임을진다. 인증수단의 발급 사무를 기기의 제조업체에서 수행하는 경우에 발급되는 인증서 자체에 문제가 발생한 경우에는 인증기관이, 발급과정에 문제가 발생한 경우에는 제조업체가 책임을 지게 된다. 더불어 종래 전자서명법에 규정되어 있는 공인인증기관에 한하여 인정되는 형식적 증거력의 인정 규정을 개정하여, 다양한 인증수단에 형식적 증거력이 부여되도록 하는 형태로 수정할 필요가 있다.

최근 관심을 받는 풍력자원개발에 대한 기초적, 실용적인 연구를 수행하고 자료를 확보하기 위해서는 실제 그 지역 주위 유동특성에 대한 지식이 매우 중요하다. 두 개의 초음파 유속계와 널리 사용되고 있는 컵유속계와 풍향기를 유동방향에 수직하게 설치하여 높이가 약 4.5m에서 풍속의 평균과 변동성분을 측정하였다. 실험 결과 레이놀즈 수는 약 6.7×105, 그리고 마찰유속, u*는 약 0.32m/s로 측정되었다. 수평 및 수직 평면 풍속에 대한 파워 스펙트럼 분석은 다양한 주파수 범위의 값들을 조합하여 넓은 주파수 범위에 대해 분석 할 수 있었다. 스펙트럼 분석 결과 4개의 지배적인 와류 에너지 피크 값을 관찰 할 수 있는데, 각각의 피크 값들은 약 1~2분, 8시간, 24시간 그리고 100시간의 주기로 나타나는 것을 확인하였다. 또한, 이 피크들 사이에 넓은 스펙트럼 갭이 0.2~10 cycle/hour 주파수 범위에 걸쳐 나타나고 있다.

The susceptibility of five stink bugs species collected soybean fields in Milyang in 2006 to seven insecticides was evaluated using electric auto-sprayer. The insecticide deltamethirn had the lowest median lethal concentration (LC₅₀) of 0.4 to 2.3 ppm regardless of stink bugs species while phenthoate had the highest concentration for one-banded stink bug (Piezodorus hybneri), green stink bug (Nezara antennata), sloe bug (Dolycoris baccarum), and brown marmorated stink bug, (Halyomorpha halys), and fenthion for bean bug (Riptortus clavatus). The average LC₅₀ for stink bugs to seven insecticides was lowest at 7.5 ppm for R. clavatus, followed by 16.6 ppm for H. halys, 17.6 ppm for D. baccarum, 19.1 ppm for N. antennata, and 28.4 ppm for P. hybneri. The relative tolerance ratio (TR), which is the TR of 90 percent lethal concentration (LC₉₀) of stink bugs to the recommended concentration, was the lowest in fipronil for R. clavatus, P. hybneri and D. baccarum, fenitrothion for N. antennata, and fenthion for H. halys. The relative average TR was lowest at 0.09 for R. clavatus, followed by 0.26 for D. baccarum, 0.30 for N. antennata, 0.37 for P. hybneri, and 0.39 for H. halys. Therefore, insecticides susceptibility was highest for R. clavatus and lowest for P. hybneri while the relative average TR was lowest for R. clavatus and highest for H. halys. Accordingly, it is expected that stink bugs can be effectively controlled with by spraying insecticides in soybean field because the relative average TR value of stink bugs was below 1.0, indicating high susceptibility of stink bugs to insecticides.

인공호수(옥정호)에서 2004년 11월부터 2007년 6월까지 월 1회 간격으로 수층별(표층: 0~5 m, 중층: 4~5 m, 하층: 8~10 m)육수학적 요인과 동물플랑크톤군집 동태 및 식물플랑크톤에 대한 섭식 기여율을 파악하였다. 조사기간 동안 수층별 평균 수온은 각각 상층 15.2±7.8℃, 중층 14.5±7.4℃ 그리고 하층은 13.0±6.2℃