The genus Tilia is characterized by linear form bracts of which the lower part is attached to the peduncle of a cyme. This character is distinguished from the others genus of Malvaceae. The purpose of this study is verifying the phylogenetic relationship of genus Tilia. Phylogenetic analyses were conducted to evaluate relationships of 10 taxa of Tilia in Korea and Japan including one outgroup (Gossypium hirsutum). The molecular phylogenetic analyses were conducted with sequences based on ITS, trnL-F and rpl32-trnL region. The combined data result of ITS, trnL-F and rpl32-trnL was formed by 6 clades. T. kiusiana situated as the most basal clade. T. amurensis, T. taquetii and T. rufa are composed a clade. T. koreana, T. insularis and T. japonica was formed independent clade. T. insularis has the closest relationship with T. japonica. T. miqueliana, T. mandshurica, and T. megaphylla are composed a clade and showed a sister relationship than other species.

Background : This text was conducted to investigate the fruit type of Camellia japonica in ko rea. Methods and Results : In fruit of Camellia japonica, For fruit length of 24.1～33.0mm was 6 3.7%, and width of 30.1～39.0mm was 60.2%, and weight of 10.1～25.0g was 62.1%. Fruit was near in circle shape, because length/width ratio 0.71～1.00 indicate 79.6%. Seed number of per fruit by weight was also tends to be which is much as fruit is heavy. In correlation b etween each of fruit character, Fruit width showed a positive correlation with the fruit length, fruit weight, seed number. Also, for seed number, the correlation coefficient is the fruit weigh t (0.643**), fruit width (0.640**) was significantly more than the length (0.532**). Conclusions : Camellia japonica was very various size, shape of fruit and seed number of per fruit.

Background : Haskap berries commonly refer to fruits of Lonicera caerulea L., recognized by the Japanese aborigines as the “The elixir of life.”. Due to their recent arrival on the North American market, haskap berries have not yet been positioned among other berries and compared in terms of their phytochemical content. And haskap berries have higher ascorbic acid and anthocyanin content than other berries known for their health-promoting benefits, such as blueberries. However, no study has reported on the antioxidant and anti-cancer activity of Lonicera caerulea stem. The purpose of this study is to present the current research on the chemical content, antioxidant and anti-cancer activities of Lonicera caerulea stem. Methods and Results : The stem of Lonicera caerulea L. ware dried in the shade at room temperature and extracted with 100% methanol. The extract was suspended in deionized water and partitioned sequentially with n-hexane, chloroform, ethyl-acetate and butanol (water saturated BuOH) fractions. Antioxidant activities were measured by determination of antioxidants, DPPH (2,2-diphenyl-1-picrylhydrazyl). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. All cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). All results were performed with three replications were processed statistically. By DPPH assay, the Lonicera caerulea L. the highest activity was obtained from the ethyl-acetate fraction (IC50=15.46 ㎍/㎖). By MTT assay, the chloroform fraction showed a significant growth inhibiting effect on MCF-7 (Human breast cancer, IC50=225.91 ㎍/㎖), COLO 205 (Human colon cancer, IC50=179.55 ㎍/㎖), but on AGS (Human stomach cancer) and other fractions it did not show effect. Conclusion : We demonstrated that Lonicera caerulea L. stem extract and fractions has antioxidant and antiproliferation activity in vitro. Further studies should identify the active constituents in Lonicera caerulea L stem to evaluate the potential in vitro antioxidant and antiproliferation activities of the extract.

Background : Yellowhorn (Xanthoceras sorbifolium Bunge) is distributed in northeastern region in China. The seeds are oil-rich and used as an edible and/or medicinal additives in China. We investigated genetic indices and molecular variance using ISSR markers and oil contents variance by analyzing fatty acid composition in several artificial populations in China. Methods and Results : Seeds were collected from four discontiguous artificial populations in four area in China : two in Inner Mongolia (IM), one in Liaoning (LN) and one in Shandong (SD). Farm in SD showed the highest number of effective alleles (Ne), Shannon index (I), and expected heterozygosity (He), i.e., 1.598, 0.470, and 0.325, respectively. Crude fat contents in kernel observed as 54.5 g 100 g-1 from SD. In contrast it was observed the lowest contents as 46.5 g 100 g-1 from LN . The fatty acid composition was determined to those of oleic acid (31.3%) from SD. And linoleic acid was determined as 38.1% from LN. These artificial populations have relatively high genetic variation, and within-population variation (23%) was higher than among populations. The artificial populations were divided into two groups, revealing these was little correlation between genetic and geographic distance. Conclusion : This study can provide the important information on genetic variation and contents characteristics. It may be responsible for the programs of improvement and germplasm conservation in the future.

Background : This study was carried out to survey the effect of IBA (indole-3-butric acid) concentration on root development characteristics in Viburnum dilatatum cuttings. Methods and Results : The cuttings were collected on July 28, 2016 from plants growing in Korea National Arboretum. Cuttings were treated with different IBA concentrations [0 (control), 10, 100, 1000, 2000 ㎎⋅L-¹] for 10 seconds. Rooting parameters (root number, the longest root length, root fresh weight, root dry weight, rooting percentage, and survival rate) were observed at 8 weeks after cuttings. Rooting percentage was 43, 33, 17, 47, and 40 % at 0, 10, 100, 1000, and 2000 ㎎⋅L-¹ of IBA, respectively. Root number (13.4) was highest under 1000 ㎎⋅L-¹ IBA. Root length (9.4 ㎝) and Root weight (fresh and dry) were highest under 1000 ㎎⋅L-¹ IBA. Conclusion : According to the results, 1000 ㎎⋅L-¹ IBA was the best treatment for increasing percentage rooting percentage and other root development characteristics of V. dilatatum cuttings.

Background : The Aralia cordata and Dendropanax morbifera, belonging to the family Araliacea, is a perennial herbaceous species. Although its beneficial effects for several chronic diseases are well-known, the role of its effects on chronic intestinal inflammation, colon carcinogenis are limited. Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of gastrointestinal tract. It has been reported that IBD is associated with colon cancer which is one of the most common types of cancer diagnosed and a major cause of cancer-related mortality. Methods and Results : The present study hypothesized that Araliacea family would exert a protective effect on inflammation and dextran sulfate sodium (DSS)-induced colitis mouse model. Effect of Aralia cordata extracts (ACE) and Dendropanax morbifera leaf extracts (DMLE) on pro-inflammatory markers, mitogen-activated protein kinase signaling (MAPKs), activation of nuclear factor κB (NF- κB) in THP-1 human monocyte and HT-29 colon epithelial cell model were investigated. In addition, colon inflammation inhibitory effects of ACE and DMLE on the characteristics of in DSS-induced colitis model. Results showed that ACE, DMLE attenuated the severity of DSS-induced colitis, as assessed by disease activity index scores, shrinkage of colon length, and histopathologic changes and infirmatory cytokine concentration. Furthermore, ACE treatment significantly suppressed the colon carcinogenesis in AOM/DSS induced colon cancer model. The apoptosis induction abilities of the DMLE were studied by analyzing the expressions of Bcl2 family protein, caspase, Annexin V/PI staining and DNA fragmentation. According to our results, DMLE significantly induced cell death in SW-480 cells. Apoptosis was determined by cell morphology and electrophoresis of DNA fragmentations. Furthermore, treatment with DMLE also induced the increase in caspase activity, pro-apoptotic proteins (Bax and Bad) and the decrease in anti-apoptotic proteins (Bcl-2 and Bcl-xL). In addition, DMLE induce cell cycle arrested at the G1/S phase in SW-480 cells and exhibited significant inhibition on the expressions of G1/S phase specific CDK, cyclin and up regulation of CDK inhibitors. Conclusion : Taken together, these results provide evidence that ACE and DMLE prevention colon cancer by regulating inflammation, colitis, and colon carcinogenesis which indicates its benefit for colon health.

Growth of 6-year old ‘Niitaka’ pear (Pyrus pyrifolia Nakai) trees and control of insect and disease occurrences were compared between fermented soybean extracts and rain-shelter system for two years. Foliar application of fermented soybean extracts was applied at 6 times as a pre-experiment in the open-field in 2013, with a rain-shelter system in 2014. Fermented soybean extract treatment increased foliar concentrations of approximately 0.46% T-N, 0.17% K, 0.19% Ca, and 0.06% Mg in 2013 compared to the control, with similar macro-nutrients between the control and soybean extract treatment observed in 2014. Rain-shelter system increased foliar concentrations of T-N, Ca, and Mg compared to the open-field. There were no significantly different between the control and soybean extract treatment for number of leaves per fruit, leaf dry weight, phytotoxicity, and completed shoot growth on August during the two years. Rain-shelter system increased leaf dry weight and did not affect phytotoxicity in the leaves. Fruit quality parameters were mostly similar to control and soybean extract treatment for two years, with higher fruit firmness observed for soybean extract treatment. Rain-shelter system advanced 4 days of harvest dates, and increased approximately 7.0 ton fruit yield per ha, 20 g mean fruit weight, and fruit soluble solid contents compared to open-field in 2014. Soybean extract treatment little suppressed occurrence of disease and insect on the leaves and fruits in both years. Rain-shelter system increased occurrence of Venturia nashicola on the leaves and to 63.8% of Gymnosporangium asiaticum on the fruits in 2014. Strong winds and storms in May elevated relative humidity in the rain-shelter system and caused high infection of the disease occurrence, requiring for an additional green control method. Soybean extract treatment little affected tree growth and would have initiated for a long-term study to evaluate tree physiological characteristics. Rain-shelter system improved fruit productivity and advanced harvest dates, which could have been more effective facility at a Thanks Giving Day between middle and end of September.

A comparative petal micromorphology of 15 taxa of tribe Sorbarieae was carried out using scanning electron microscopy (SEM) in order to evaluate their taxonomic and systematic implication. The characteristics of both adaxial and abaxial side of petal epidermal cells such as shape, arrangement, anticlinal wall and cuticular sculpture were described in detail. The Sorbarieae petal epidermal cells were identified as papillate or conical papillate (PCS) and rectangular-rugose tabular (TRS). Three types of petal micromorphology were found within the tribe based on the cell patterns of adaxial and abaxial side. Type I. PCS-TRS, Type II. PCS-PCS, Type III. TRS-TRS (adaxial-abaxial side). The petal epidermal cell types, anticlinal cell wall, and cuticular sculpture represent not only taxonomical importance for identification between or within the genera but also systematic implication. Moreover, this study provides a relation between petal epidermal cell pattern and insect pollinator (entomophily; melittophily) as an additional case.

Background: This study was conducted to investigate the effects of germanium treatment on the growth and organic germanium production in the roots of Oplopanax elatus plantlets. Methods and Results: O. elatus plantlets were cultured in Murashige and Skoog (MS) medium with different concentrations of germanium dioxide (GeO2) to analyze optimum growth conditions. Exogenous treatment of 10 ㎎/ℓ GeO2 promoted growth and an increase in the contents of chlorophyll a, b and carotenoid in O. elatus. The germanium accumulation and production in roots of O. elatus plantlets treated with organic germanium reached the highest levels. The growth of the aerial and underground portion of O. elatus with organic germanium was greater than that of the control. The accumulation and production of organic germanium reached the highest level (40.89 ㎍/plantlet) with the treatment of 50 ㎎/ℓ GeO2. Antioxidant activity measured by DPPH and ABTS assays also increased with the germanium treatment and improved the DPPH and ABTS radical activity by 200% compared with that in the control. In addition, the total phenol and flavonoid contents of the plantlets with a treatment of 50 ㎎/ℓ GeO2 were higher than in the control. Conclusions: Taken together, the growth of O. elatus was increased with the treatment of 50 ㎎/ℓ GeO2 germanium and the biological references improved, with increased antioxidant activity and organic germanium production.

Birds play a main role in the formation and change of forest structures as they are seed-dispersal agents. This study aims to identify birds consuming fruits and seeds of the tree and their associated fruits and seeds on Jeju Island in the context on the forest restoration in Korea. We conducted field surveys twice a month from 2013 to 2015 at nine study sites located across Jeju Island and collected available photographic and observation records. A total of 50 species of birds consuming fruits and seeds of the tree were identified and birds belonging to Bombycillidae, Pycnonotidae, Zosteropidae, Sturnidae and Fringillidae were confirmed as major birds consuming fruits and seeds of the tree. Gulping was the dominant type of feeding as well as crushing, but relationship between the size of a bird and the number of fruit and seed species used by the bird was not significant. We also documented that 118 fruit and seed species were consumed by birds and that shrubby fruits and seeds were more consumed by birds than those of other plant types. The relative consumption rate of fruits and seeds ranged from 0.02 to 0.44, but five species were the most important fruits and seeds for birds. Our finding suggest that avian frugivorous gulpers will benefit the seed dispersal, especially of five fruiting plants, providing useful baseline data for forest restoration and urban park design.

Background : This study was conducted to developed the propagation method by cutting for mass cultivation of Vitex roundifolia. We were pitched the cutting two times and treated plant growth regulators to enhance the rooting percentage. Vitex roundifolia is live in beach sandy soil south of Hwanghae-Do and Gangwon-Do. Vitex roundifolia have been used to bath foam. It is good for aromatic plant. It has 0.8% essential oil content in leaf and flower. Major components of essential oil were alpha-Pinene, Sabinene, beta-Pinen, 1,8-cineole, d-Limone. Despite the superior usability, it had not yet been made by the artificial cultivation Methods and Results : We were pitched the cutting of a first-year branch on June 5, which was greenwood cutting and July 17, which was semi-hardwood cutting at Kwangseung-ri beach, Gochanggun, Jeonbuk. The length of cutting was 10cm. It had 3～4 nodes, we stuck a cutting remain 2 nodes above ground on ordinary raise seedling soil. Rooting percentage was measured at 60 days after stuck a cutting. Rooting percentage was higher greenwoody cutting(95%) than semi-hardwood cutting(57.6%). In green wood cutting, there was no significance with plant growth, but chemical injury was occurred in IBA 5,000ppm. In semi-hardwood cutting, there was significance with plant growth regulators. The rooting percentages of all the treat were higher than control(no treatment). Rooting percentage was the highest in NAA 5,000ppm treated. Conclusion : Greenwood cutting method was more proper to propagation for Vitex roundifolia than semi-hardwood cutting. The optimum time to cutting for Vitex roundifolia propagation was the early in June. If miss a time to propagation Rooting percent was elevated by plant growth regulator.

피부 손상은 주로 자외선, 열, 담배 등과 같은 환경적 요인으로부터 초래되는데, 이는 활성산소종의 과생 성으로 인한 피부노화와 연관이 있는 것으로 알려져 있다. 돌배(Pyrus pyrifolia NAKAI)는 전 세계적으로 많이 소비되는 과일로써 항암, 항산화, 항염증효과가 알려져 있다. 본 연구에서는 돌배나무잎 추출물(Pyrus pyrifolia leaf extract, PPE)의 ultraviolet B (UVB)스트레스에 대한 피부 섬유아세포 보호효과를 검증하였다. Lactate dehydrogenase assay와 DCF-DA를 이용한 정성분석 실험은 PPE가 인간의 섬유아세포에서 UVB 스트레스 에 의해 유발된 세포독성 및 과생성된 활성산소종을 농도 의존적으로 억제할 뿐만 아니라, 미토콘드리아 기능저 하, 막전위 저하, 그리고 세포사멸과정의 핵심 인자인 caspase-3 활성도 유의하게 억제함을 보여주었다. 결론적 으로, PPE는 UVB스트레스에 의해 과생성된 활성산소종을 억제시켰으며, 이로 인해 생기는 피부세포 사멸을 효과적으로 저해함을 확인하였다.

Background : Acer pictum is a deciduous tree belonging to the family Aceraceae. This study was conducted to collect basic data on mass propagation techniques by surveying the germination and growth characteristics of A. pictum seeds. Methods and Results : A. pictum seeds were harvested in 2014 on August 19, September 5, September 29 and October 31. The seeds were then sown on the same dates they were harvested. A portion of seeds harvested in August 2014 were stored at low temperatures and subsequently sown on March 11, 2015. The germination rate of A. pictum seeds was highest in seeds that were stored harvested on October 31. Mean germination time (MGT) was shortest and germination velocity (Rs) was fastest in seeds harvested on October 31. Root collar diameter, total root length, and dry weight were also highest from seeds collected October 31. Conclusions : A. pictum seeds harvested on October 31 had highest germination and growth rates compared with seeds harvested on earlier dates. It is expected that these results demonstrating optimum harvesting and sowing dates will be applicable to future seedling production for this tree species.

This study’s purpose was to provide basic data for the monitoring of ecological changes caused by change of vegetation structure of Abies koreana forest in a study site susceptible to climatic change in Yeongsil area of Hallasan Mountain, Jeju Island. Surveys revealed this: in Yeongsil area of Hallasan Mountain, per 1 ha of A. koreana forests, total number 1,781, and A. koreana number 989, accounting for 55.5% of the total number of trees. 190 A. koreana or 19.2% were found to be dead. For the number of individual trees by DBH, trees standing 5 ㎝ -10 ㎝ tall formed the largest portion at 39.9%, and in the case of other trees except A. koreana, the number of individual trees below 5 ㎝ accounted for 23.5% of the total number of trees. The survey of importance by height revealed this: at the top level, the importance of A. koreana was the highest at 106.23, but the sum of importance of temperate deciduous broad-leaved trees (Prunus maximowiczii, Quercus mongolica, and Taxus cuspidata) was higher at 142.84 than that of A. koreana. The analysis of species diversity revealed 0.645 species diversity for the tree layer and 0.817 for the shrub layer; for evenness, 0.549 for the tree layer and 0.664 for the shrub layer; for dominance value; 0.451 for the tree layer and 0.336 for the shrub layer. The analysis of tree vitality revealed that for the A. koreana forests in Yeongsil, the composition ratio of A. koreana by type is AS type>AL type>DS type >DB type, and that of the other trees is AL type>AS type>AF type>AB type. Compared with the forests in other areas, the A. koreana forests in the Yeongsil area have a very high occurrence rate of dead trees, and a high importance of trees is shown in the deciduous broad-leaved tree forests. Compared with the A. koreana forests in the Jindallaebat area, with the same level above sea, the vegetation structures are fast changing. Also, due to dryness and other non-physical environmental changes caused by a lack of rainwater and dry winds in winter, dead trees are fast increasing in number. Environmental changes such as climate change diversely affect the maintenance of A. koreana in individual areas, and if environmental changes are fast and continue long, of the A. koreana forest areas in the Hallasan Mountain, the A. koreana forests in the Yeongsil area will decrease fastest in number and will experience changes in the vegetation structure. Thus, it is necessary to survey the vegetation changes in A. koreana forests, which are distributed in all directions but are centered on Hallasan Mountain, and to thus conduct long-term monitoring and research.

Total phenolic compounds and antioxidant activity were investigated in leaves of organic apricot, filbert, mulberry, persimmon, and pomegranate trees during growing season. Total phenolic compounds and antioxidant activity in leaves of organic apricot and filbert trees were gradually increased from May to June, attained peak in July, thereafter decreased to minimum level in October. In leaves of organic mulberry tree total phenolic compounds and antioxidant activity were simultaneously increased from May to June and sharply decrease to very low level. The leaves of organic persimmon trees showed very high level of total phenolic compounds and antioxidant activity from May to June, thereafter gradually decrease to October. The leaves of organic pomegranate trees showed extraordinarily highest level of total phenolic compounds among five fruit trees investigated in the study during growing stages, as well as maintained higher than 91 percent of antioxidant activity from May to October. Correlation coefficients between total phenolic compound and antioxidant activity of the leaves of five organic trees were the highest in mulberry leaves, and then persimmon, filbert, apricot, and pomegranate, respectively. However, correlation coefficients between total phenolic compound and antioxidant activity of the leaves of organic pomegranate were very low level, and not significant in their relationship.

Background : Chinese matrimony vine (Lycium chinense Mill.) is a deciduous shrub belonging to the Solanaceae. The leaves are used as an alternative raw material replacing dried fruits associated with high production costs in many industires. The aim of this experiment was to determine the effect of drying temperature on the leaves used in the manufacture of functional products. Methods and Results : The leaves of Chinese matrimony vine were harvested when the plant height reached 60 - 70 ㎝ in the spring and treated at four different temperatures (40℃, 50℃, 60℃ and 70℃). The time to reach the desiccation state of 20% was 63 h at 40℃, 37 h at 50℃, 17 h at 60℃ and 11 h at 70℃. The drying rate per hour was 1.28% at 40℃, 2.25% at 50℃, 4.94% at 60℃ and 7.60% at 70℃. No significant difference were observed in ash, crude fat, polyphenol or rutin content of treated samples. Crude protein and betaine content decreased with higher dry temperatures. Nitrogen free extract of the treated samples increased with higher drying temperatures. Conclusions : Taking into consideration drying time, drying rate, color value, energy consumption and functional ingredients is advantageous to dry the leaves of the Chinese matrimony vine at 60℃ using a hot air agricultural dryer.

‘장원’은 국립산림과학원에서 당도가 높으면서 종피가 잘 벗겨지는 과실 특성을 가진 품종을 육성하기 위해 수령 60년 생 이상의 노거수를 대상으로 선발하여 개발한 품종이다. 이 품종은 2001년에 처음으로 선발되었고 2007 ~ 2009년까지 과실 특성에 대한 안전성 검정을 수행하였다. 특히 이 품종의 수확시기는 9월 둘째주이며, 8년생을 기준으로 나무당 수확 량은 약 3.9 kg이다. 이 품종은 과실 특성으로 당도 17.5%, 경도 9.6 kg/cm2, 종피 박피율 98.6%로 대조 품종인 유마 보 다 과실품질이 우수하여 군밤용으로 특화되어 이용 될 수 있 다. 또한 다른 품종의 종피 박피율 향상을 위한 수분수로도 활용 가능성이 크다.

The objective of this study was to investigate anticytotoxic and antioxidatative capacities of ethanol extracts from Acer tegmentosum Maxim (A. tegmentosum) stem in vitro. The extract at concentration of 200 ug/mL inhibited 10 and 20 ug/mL arsenic trioxide-induced cytotoxicity of HepG2 cells by 79.3 and 57.5%, respectively. The extract at concentration of 200 ug/mL inhibited 0.2 and 0.5 mM t-BHP-induced cytotoxicity of HepG2 cells by 66.3 and 35.7%, respectively. Antioxidative effects of the extract were examined via measurement of ABTS, superoxide, and peroxyl radical scavenging activities. ABTS radical scavenging activity of the extract was higher than that of α-tocopherol. Superoxide scavenging activity of the extract was higher than that of catechin. Oxygen radical absorbance capacity of the extract was higher than that of ascorbic acid. Cupric reducing antioxidant capacity of the extract was higher than that of α-tocopherol. The extract at concentrations of 100 and 500 μg/mL inhibited 10 mM t-BHP-induced lipid peroxidation of HepG2 cells by 38.2 and 80.7%, respectively. The extract prevented supercoiled DNA strand breakage induced by hydroxyl or peroxyl radical. Total phenolic and flavonoid contents of the extract at concentration of 100 μg/mL were 71.3 nmol/mL gallic acid and 18.8 nmol/mL catechin equivalents, respectively. Thus, strong cytoprotective and antioxidant effects of A. tegmentosum stem extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in polyphenolic contents.

In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.

Here we report the protective activity of cultured Acer tegmentosum cell extract against liver damage in rat intentionally instigated by D-galactosamine. Local fat degeneration and infiltration of inflammatory cells were significantly decreased in cultured A. tegmentosum cell extract administered rat. In addition, acutely increased AST, ALT, LDH, ALP activities and lipid peroxidation and lipid content by liver damage were recovered in experimental rat administrated with A. tegmentosum extract. These results showed that cultured A. tegmentosum cell extract has a role in blood enzyme activation and lipid content restoration within damaged rat liver tissues. Moreover expression rate of TNF-α which accelerates inflammation and induces tissue damage and necrosis was significantly decreased. Also activities of antioxidant enzymes were more effectively upregulated comparing to those of the control group induced hepatotoxicity. All data that cultured A. tegmentosum cell extract has a preventive role against liver damages such as inflammation, tissue necrosis in rats by improving activities of blood enzymes, antioxidant enzymes and modulating expression of inflammation factor, suggest that cultured Acer tegmentosum cell extract is an effective medicinal resource for restoration of hepatotoxicity.