This study investigated ginseng growth and ginsenoside contents after control a reserved ginseng cultivation land using various green manure crops for stable ginseng cultivation. Followings are results obtained from this research. After cultivate the green manure crops, microbial flora in soil was diversified, organic matter and total-N content increased, but salt content decreased. The highest output obtained from the wheat cultivated area among various green manure corps. Growth of shoot and root of two years old ginseng increased significantly at the green manure crop cultivated area. Specially, the wheat cultivated area was the most effective in growth. Also, the rate of the leaf discoloration at the aerial part and the rusty root at the root was the least at the wheat cultivation area. Meanwhile, the ginsenoside content was the most at the wheat cultivation area. Thus, the reserved ginseng cultivation land could be managed by cultivating wheat for effective ginseng growth.

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.

This study was conducted to select and develop effect of eco-friendly organic materials for the eco-friendly prevention of Anthracnose occurred in the ginseng. Anthracnose on ginseng is occurred by Colletotrichum gloeosporioides and the crop damage is severe especially from July to August after rainy season. The test results showed that control effect by test products materials on the three years ginseng and four years ginseng field was lower in eco-friendly organic materials than that of chemical pesticide. However, the control effect of bordeaux mixture was higher with 71.3% and 73.8% levels than those of mineral matter, microbial agent, and developed plants extract mixtures (Eugenol, Curcumin, Wood vinegar, etc). On the other hand, three types of developed plants extract mixtures (3 types) showed control effect in a range of from 58.1% to 63.6% against Anthracnose which was higher as compared with plant extract alone and sodium silicate regardless of ages of ginseng. The results of this study would attribute in verifying the control effect of eco-friendly materials against Anthracnose for ginseng through investigating antimicrobial compounds contained in the plants body. Also, it would be used as control method against Anthracnose occurred in ginseng by judging the right control time through monitoring occurrence of disease.

The average and maximum temperature were 29.5℃ and 33.2℃ at 2:00 p.m. respectively, in the plastic-filmhouse covered with shade net, and both of temperature were lower 0.6℃ and 1.3℃ than those of conventional shade. Lighttransmittance was 14% in the plastic-film house, while 9.9% in conventional shade during growing season from May toOctober. Withering time of aboveground part was on October 3rd in conventional shade with 60% of withering leaf, while itwas on November 10th with 3.7% of withering leaf in the plastic-film house, about 40 days longer survival. The main diseaseincidence were 15% of anthracnose, 17% of leaf spot, 5% of phytophthora blight and 3% of gray mold in the conventionalshade, while 0~0.1% disease incidence and 95% of emergence rate in the plastic-film house. The growth in the above-ground and underground part of ginseng was totally better, particularly characteristics affecting yield such as root length,main root length and diameter in the plastic-film house . The fresh weight was increased by 128% compared to the conven-tional shade and harvested roots per 3.3㎡ were 36 roots in the conventional shade and 58 roots in the plastic-film house andfuthermore yield per 3.3㎡ was increased by 216% compared to the conventional shade. As covering materials, the ricestraw in the plastic-film house was excellent. The ginsenoside contents affecting the quality of ginseng were higher in theplastic-film house indicating 0.333% of Rg1, 0.672% of Rb1, 0.730% of Rc and rate of red rusty root was less than4.0~6.1%. Above the results, the quality of ginseng grown in the plastic-film house covered with shade net was improvedthan that of the conventional shade.