Photodynamic therapy(PDT) is recently developed as an effective treatment for malignant disease. Carboplatin, a less nephrotoxic analog of cisplatin, has been widely used for the treatment of multiple malignancies. In this study, we investigated the cytotoxic and apoptotic effect of combined modality of photofrin mediated PDT with cisplatin and carboplatin on KB cell human oral cancer cell line in vitro. The a ttached KB cells were incu bated with c isplatin(0.04mg/ml) and carboplatin(0.02mg/ml) for 24h at 37℃ and followed by photosensitization with photofrin for 6h and laser irradiation with 630nm LED at an intensity of 2.0 J/cm2 for activating photofrin for 15min. Then MTT assay and SYTO 16 green & Propidium iodide (PI) double staining were used respectively to measure the cytotoxicity and nuclear morphology at 24h after PDT. This study demonstrates that the combined modality with carbopaltin resulted in enhanced apoptotic cell death as well as cytotoxic e ffect on KB c ells in vitro, which s uggests the feasibility of combined modality and the possibility o f reducing the effective dosage of photofrin and carboplatin and lowering the side effects on normal cells

경기도 의왕시 포일동의 후기 플라이스토세 퇴적층에 대한 화분분석 결과, 초본류 및 양치식물로 이루어진 저층의 발달과 함께 침엽수-낙엽성 활엽수 혼효림이 분포했던 것으로 나타났다. 기후조건을 반영하는 식생조성의 변화가 인지되는데, 약 43,100년 전-41,900년 전 시기에는 오늘날보다 다소 추운 한랭한 온대 기후하에서 초지식생을 수반한 침엽수-낙엽성 활엽수 혼효림이 발달하였다. 약 41,900년 전-41,200년 전 동안에는 양치식물 저층의 발달과 함께 침엽수-낙엽성 활엽수 혼효림 내에서의 아한대성 침엽수의 감소와 온대성 낙엽 활엽수의 증가가 나타나는데 이는 기후조건의 온난화를 지시한다. 약 41,200년 전-39,700년 전 시기에는 기후 한랭화에 따른 아한대성 침엽수의 증가와 식생밀도의 감소가 나타난다.

This study investigated the perception, evaluation, and the possibility for globalizing Korean foods and Yak-sun among different nationalities of foreigners living in Korea. A survey was conducted with 171 foreigners (eastern Asian, southeastern and central Asian, European, and American). The questionnaire was composed of three parts, including perception, evaluation, and the possibility for globalizing Korean foods and Yak-sun. The reason for choosing Korean foods cited by southeastern and central Asians and Americans was "taste", whereas eastern Asians and Europeans chose "culture" and "curiosity", respectively. Americans and Europeans considered "spiciness" as a reason not to choose Korean foods (P<0.001). Regarding the possibility of globalizing Korean foods, eastern Asians and Americans/Europeans/southeast Asians and central Asians responded "Korean dining culture" and "incomprehensive menus", respectively (P<0.05). An "explanation of the menu to foreigners" was regarded as the main suggestion for globalizing Korean food. Most of the respondents understood that Yak-sun is an oriental medicinal food that provides improved health and disease prevention. A number of Europeans and Americans expressed high interest in the conceptualization of Yak-sun (P<0.001). With regard to the priority for developing Yak-sun, eastern Asians, southeastern and central Asians, and Europeans picked out "health status improvement", whereas "improved nutritional status" was manifested in the majority of the American's responses (P<0.001). Therefore, it is crucial that "localization" be applied to Korean foods and Yak-sun to meet the international standard. Furthermore, it is necessary to provide simplified and correct Korean food content information to foreigners.

한우 이력추적제에 적용되는 11개의 MS marker (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, ETH3, ETH225, BM1824 and INRA23)와 성감별을 위한 2개의 sexing primer로 조합된 하나의 Multiplex PCR set를 이용하여 모근에서 추출한 genomic DNA를 이용해 3510두의 대량 시료를 분석한 결과 3.93%의 genotyping 실패율로 성공적인 분석결과를 얻었다. 무작위교배집단으로 가 정 시 동일개체출현확률 (PI)은 1.31×10-23, 반형매교배집단으로 가정 시 동일개체출현확률 (PIhalf-sibs)은 2.52×10-16 그리고 전형매교배집단으로 가정 시 동일개체출현확률 (PIsibs)은 1.09×10-6으로 나타나 현재 사용 중인 11종의 MS marker는 범용적으로 사용하여도 무방할 것으로 재확인 되었다. 또한 생산 및 사 육단계의 생우의 경우 모근을 이용하여 DNA를 추출하는 것은 시료 채취 시 소에게 주어지는 스트레스를 최소화 시킬 수 있을 뿐만 아니라 대립유전자형 분석에 있어서 시간적, 경제적인 효율성을 높일 수 있었 다. 또한 모근 채취 부위 중 등, 배, 꼬리상부와 꼬리하부를 이용하여 검정한 결과 꼬리하부의 모근을 이 용하여 5~13가닥을 사용했을 때 최적의 분석결과를 보였다. 최종적으로 한우의 사육단계 대량 유전자형 분석에 적용 가능한 96 well 단위를 기본으로 하는 모근 DNA분리 체계를 확립하였다.

자치입법의 활성화는 자치입법의 양적 증대와 질적 우수성의 제고를 동시에 의미한다고 할 수 있는데, 자치입법의 양적 증대와 관련하여서는 규율가능한 대상사무의 확대가 가장 우선적으로 필요하다 할 것이며 질적 우수성을 제고하기 위해서는 상위법령과의 적법한 체계의 형성과 함께 지방자치단체의 개별적 상황을 잘 반영하고 지역의 당면과제에 기능할 수 있는 자치입법의 형성이 요구된다 할 것이다. 이 연구에서는 자치입법의 양적 증대와 질적 우수성을 제고하기 위해서는 입법과정의 절차를 통한 사전적 조정의 중요성이 크다는 인식하에 절차법적 문제를 중심으로 검토를 수행하였다. 즉 크게는 자치입법의 제정 등의 과정에 있어서의 주민참여절차와 자치입법과 관련하여 지방자치단체가 중앙정부와 국회에 의견을 제시하고 영향력을 행사할 수 있는 절차로 나누어 고찰하였다. 우리의 조례제정개폐청구제도는 매우 엄격한 절차를 요구함에도 불구하고 실질적인 영향력은 매우 약하다는 점이 지적될 수 있는데, 이러한 문제점의 개선을 위해서 주민투표와의 연계, 청구요건의 완화, 청구대상의 확대, 심의단계에서의 절차적 권리의 보장, 이의제기권의 보장 등의 문제가 개선될 필요성이 있다. 또한 지방자치단체 역시 국가 내적 존재이기 때문에 헌법과 법률에 의한 한계 내에서만 입법권한이 인정되고 행사할 수 있고, 따라서 자치입법권한의 인정과 행사에 있어 발생하는 법적 문제점은 지방자치단체가 중앙의 입법과정에 참여할 수 있는 절차적 보장이 충분히 이루어짐으로써 어느 정도 해소될 수 있을 것이다. 현행 법제는 아직 이러한 절차를 충분히 보장하고 있지는 않으며, 입법적 개선이 요구되는 부분이라 하겠다.

본 시험은 섬유질배합사료(TMR)의 에너지 및 단백질 수준이 다를 경우, 비유초기 유산양의 사료섭취량 및 유생산에 미치는 영향을 조사하기 위하여 수행하였다. 시험 동물은 자넨종 생후 16개월령 전후의 초산 착유양 24두를 시험구별 6마리씩 공시하였으며, TMR의 에너지와 단백질 수준은 대조구(Control)가 TDN 67.0%, CP 13.9% (NRC, 2007), 에너지 증량구(T1)는 TDN 73.7%, CP 13.9%, 단백질 증량구(T2)는 T

This study was carried out between 2008 and 2009 in four dairy farms to investigate the effect of feeding of whole crop barley silage on the reproductive performance of Holstein heifers. Two diets, mixed hay or whole crop barley silage separately from concentrate were fed 6-month old Holstein heifers (=37). In control group (=CON), heifers (n=16) were fed 6 kg (/head) mixed hay and 4 kg (/head) commercial diet. In whole crop barley silage group (=WBS), heifers (n=21) were fed 10 kg (/head) whole crop barley silage, 4 kg (/head) mixed hay and 2 kg (/head) commercial diet. To manage body weight gain, the body condition score of heifers were measured every month. The results obtained were as follows: 1. Body weight in CON and WBS heifers at 13-, 14-, 15- and 17-month old were 340±17.9 and 342±13.6 kg, 356±15.7 and 366±14.7 kg, 382±13.1 and 387±14.4 kg, and 429±15.0 and 417±10.3 kg, respectively. 2. Body condition score in CON and WBS heifers at 9-, 12-, 15- and 17-month old were 2.88±0.04 and 2.80±0.04, 2.88±0.04 and 2.80±0.04, 2.89±0.08 and 3.00±0.07, and 2.89±0.08 and 3.00±0.07, respectively. 3. Average age of sexual maturity in CON and WBS heifers were 437.3±9.9days and 939.6±12.5days, WBS group heifers were significantly shorter (p<0.05) than CON group heifers. 4. First-service conception rates in CON or WBS group were 81.3% (13/16) and 66.7% (14/21), respectively, and cumulative conception rate to 2nd artificial insemination were 87.5% for CON and 85.7% for WBS group. Conception rate was not different between treatments

Purpose : We studied the relationship of dominant eye and fixing eye in heterophoria and heterotropia. Methods : Fifty-one subjects(Male: 21, Female; 30) were measured between seven to sixty three years, evaluated to alternate cover test after AR/K(Canon RK-Fl, Japan) measw·ed. Ocular dominance was determined using hole in-the-card test before measured in subjective refraction. Prism bar was determined using objective deviating tropia angle after refraction. Results : Intermitant heterotropia patients were the most 26 patients(Sl%) in binocular anomalies, equaled to dominant eyes and fixing eyes were 28 patients(55%), non equaled to dominant eyes and fixing eyes were 23 patients(45%). Alternated dominant eyes were searched in esotropia and exotropia each to one patient, esophoria patints were nothing. Dominant eyes had greater degree of myopia than nondominant eyes but show no significant difference between eyes(p=0.95), fixing eyes had lesser degree of myopia than nondfixing eyes but show no significant difference between eyes(p=0.88). Conclusions : The analysis showed that using a combination of dominant and fixing eye test because small deviating eyes would be likely to different from dominant eye to fixing eye, alternated fixing eyes.

The objective of this study was to determine physiological and mechanical factors effecting on the pregnancy rates following artificial insemination in dairy cattle. Estrus for artificial insemination of cows was checked whether the outflow of mucus from the elements was out flow or not. There were no significant difference of gestation rates with mucus release (31.76%) and without mucus release (24.03%). The pregnancy rates were 39.02% in 10~20mm of follicle size group and 27.08% (p<0.05) in 20~30mm diameter of the follicles. There were not different pregnancy rates between twice inseminated dairy cattle and more than 3 times inseminated cattle. The pregnancy rate was 28.57% under automatic milking system. In contrast, under artificial milking system pregnancy rate was 56.85%. Two systems significantly (p<0.05) were different. These results suggest that pregnancy rates were not effected by physiological system, but mechanical condition.

21세기 지식기반사회에서 기술 발전 속도는 가속화되고 있으며 조직 목표를 달성하는데 영향을 줄 수 있는 시장, 거버넌스 및 사회적 가치의 미래 모습에 대한 불확실성이 증대하고 있다. 이러한 기술과 사회 발전의 가속화 및 증가하는 불확실성에 대응하기 위해서 다양한 미래사회 전망을 바탕으로 미래유망기술을 도출하는 기술예측의 필요성은 더욱 중요해지고 있다. 기술예측에는 다양한 예측 방법론이 이용 가능하나 예측의 목적 및 재원 등에 따라 선택되어야 한다. 델파이 방법이 오랫동안 주로 사용되어 왔지만 최근에는 시나리오 또한 많이 활용되고 있다. 시나리오는 사회, 경제 및 정치 등의 환경요인의 복잡성과 불확실성을 폭넓게 고려할 수 있으며 미래의 다양한 모습을 이야기 식으로 전달하기 때문에 매우 효과적인 전략적 도구로 활용된다. 전세계적으로 진행되고 있는 기후변화, 화석연료의 고갈 등으로 세계 각국은 신재생에너지에 대한 높은 관심을 갖고 기술개발 및 보급 등을 적극적으로 추진하고 있다. 하지만 신재생에너지 분야의 가용잠재량은 지역적으로 큰 편차가 있으며 기술적 진보, 환경규제 및 화석 연료의 가격전개와 밀접하게 연관되어 있어 그 발전추이를 예측하기 어려운 면이 있다. 이에 본 연구는 이러한 불확실성을 반영하여 신재생에너지 분야에 대한 다양한 미래 시나리오를 작성하고 시사점을 도출하였다. 향후 본 연구의 시나리오 기반의 예측 프로세스를 국가과학기술예측에 활용함으로써, 기존의 델파이 위주의 단정적 예측의 단점을 보완한 전략적 예측을 강화할 수 있을 것으로 전망된다.

Hybridization of semiconductor materials with carbon nanotubes (CNTs) is a recent field of interest in which new nanodevice fabrication and applications are expected. In this work, nanowire type GaAs structures are synthesized on porous single-wall carbon nanotubes (SWCNTs) as templates using the molecular beam epitaxy (MBE) technique. The field emission properties of the as-synthesized products were investigated to suggest their potential applications as cold electron sources, as well. The SWCNT template was synthesized by the arc-discharge method. SWCNT samples were heat-treated at 400˚C under an N2/O2 atmosphere to remove amorphous carbon. After heat treatment, GaAs was grown on the SWCNT template. The growth conditions of the GaAs in the MBE system were set by changing the growth temperatures from 400˚C to 600˚C. The morphology of the GaAs synthesized on the SWCNTs strongly depends on the substrate temperature. Namely, nano-crystalline beads of GaAs are formed on the CNTs under 500˚C, while nanowire structures begin to form on the beads above 600˚C. The crystal qualities of GaAs and SWCNT were examined by X-ray diffraction and Raman spectra. The field emission properties of the synthesized GaAs nanowires were also investigated and a low turn-on field of 2.0 V/μm was achieved. But, the turn-on field was increased in the second and third measurements. It is thought that arsenic atoms were evaporated during the measurement of the field emission.

PDT is an established cancer treatment modality. This can be attributed to the attractive basic concept of PDT; Combination of two therapeutic agents, a photosensitizing drug and light, which are relatively harmless by themselves but when combined, cause more or less selective tumor destruction. Hematoporphyrin-derived photosensitizers are known to be stable and highly efficient. In this study, we conducted a series of experiments to develop light-induced anticancer drugs against oral cancer cells. We tested the cytotoxicity of photodin by MTT assay and observed cell death pattern (apoptosis or necrosis) by hoechst 33342 and propidium iodide staining methods after PDT. IC50 value of photodin was 0.65 ug/ml. At higher doses of photodin ( > 7.8 ug/ml), cancer cells died exclusively from necrosis after PDT. By contrast, at IC50 value, photodin induced cancer cell to undergo apoptotic cell death. The induction begins approximately 6 hours after PDT. We investigated intracellular localization of photodin by oral cancer cell via confocal laser scanning microscopy. Oral cancer cells dual-stained with photodin and organelle-specific fluorescence probes (Mitotracker, Lysotracker, ER-Tracker) revealed that an intracellular fluorescence distribution was restricted to cytoplasmic compartments with no detectable fluorescence in the nucleus. Confocal images of cells containing photodin were overlapped with the mitochondria-specific fluorescence probe images of the same cells. These results demonstrated that photodin may play the role of a photosensitizer for oral squamous cancer cells without swelling and inflammation. Therefore, photodin-based PDT is a suitable treatment for oral cavity carcinoma patients.

The purpose of this research was to investigate whether the perception of KPIs by restaurant managers from financial and non-financial perspectives was affected by BSC implementation. The perceptions that were examined were importance, adoption, performance, and utilization of KPIs. We surveyed managers from multinational restaurant chains that were adopting BSC and those that were not. From a non-financial perspective, the difference in perceived importance between BSC adopted firms and firms that did not adopt BSC was significant. The managers of BSC adopted firms perceived KPIs more seriously than the others. Secondly, according to the managers' working experiences, the difference of perceived utilization in the internal business process perspective was significant between BSC adopted firms and firms that did not adopt BSC. In addition, from the learning and growth perspective, the difference in perceived adoption and utilization between the two groups was significant. Finally, in the BSC adopted firms, the perceived importance of the managers affected the other perceptions like adoption and utilization from both the financial and non-financial perspectives.

Polysulfone막의 효율을 향상시키기 위하여 막에 브롬화 시킨 후 막의 구조 및 기체투과 특성에 대해 연구하였다. 막의 특성은 FT-IR, 1H-NMR, TGA, Contact angle 등을 사용하여 조사하였고, 브롬화된 막의 기체투과 특성을 알아보기 위해 He, N2, CO2, O2를 사용하였다. 브롬화된 Polysulfane막의 기체투과결과 브롬의 양이 증가할수록 투과도는 감소하고 투과 선택도는 증가하는 결과를 얻을 수 있었다. 그 중 질소는 불소화에 따라서 가장 큰 영향을 받아 투과도가 최고 36%까지 낮아지는 결과를 나타내었으며, 이산화탄소에 대한 질소의 투과선택도는 15% 이상의 매우 향상된 결과를 나타내었다.