Microbial proteases are more economical than plant- and animal-derived proteases due to their ease of production and high activity. This study aimed to optimize the production of proteases from fermentative food-derived microorganisms. Five strains with proteolytic activity among 50 Bacillus sp. were first screened. Two strains with high protease activity were identified: Bacillus amyloliquefaciens SRCM 102139 and Bacillus subtilis SRCM 104999. SRCM 102139 strain and SRCM 104999 strain had the highest protease activity in 0.8% glucose and 0.3% yeast extract, and in 0.8% starch and 0.1% soy peptone, respectively. The production of protease for two strains was optimized by the Central Composite Design (CCD) under response surface methodology. The optimal conditions for protease production in SRCM 102139 were 0.5% and 0.347%, pH 6.0, for carbon (glucose) and nitrogen (yeast extract springer 0202) sources, respectively, with a predicted value of 0.929 U/mL. Additionally, the optimal conditions for protease production in SRCM 104999 were 0.5% and 0.5%, pH 6.7, for carbon (starch) and nitrogen (soy peptone HSP-349) sources, respectively, with a predicted value of 0.431 U/mL. The actual protease activities of SRCM 102139 and SRCM 104999 under the established conditions were 0.926 U/mL and 0.428 U/mL, respectively, closely matching the predicted values.

이 논문은 정적 재하상태에 있는 무피복 강합성보와 내화피복을 적용한 강합성보를 대상으로 화재 시 내부 온도 및 수직처짐에 대 한 내화피복의 영향을 평가한 결과를 제시한다. 열응력해석을 위한 화재하중으로는 American Society for Testing and Materials E119 의 표준화재곡선을 사용했으며, 강재거더 표면에 부착하는 내화재료의 방화효과를 구현하기 위해 외기에서 강합성보로 전달되는 열 의 전달계수를 감소시켰다. 실규모 무피복 강합성보에 대한 구조화재실험에서 내부 온도분포와 수직처짐을 측정하였고 실험 결과와 의 비교를 통해 비선형 구조화재해석 결과의 타당성을 검증하였다. 내화피복이 적용된 강합성보의 구조화재해석 결과로부터 강재거 더 표면에 내화재를 적용할 경우 동일 화재 조건에서 무피복 강합성보에 비해 내부 온도와 수직처짐이 감소함을 알 수 있었다. 또한 열 전달계수의 변화에 따른 열응력 응답으로부터 화재 시 강합성보의 온도 및 구조거동에 대한 내화피복의 영향을 제시하였다.

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 μmol CO2·m-2·s-1 on the 10 days, gradually increasing to 21.3 μmol CO2·m-2·s-1 on the 19 days, and reaching 23.4 μmol CO2·m-2·s-1 on the 32 days. After that, it lowered to 16.8 μmol CO2·m-2·s-1 on the 38 days and dropped significantly to 7.6 μmol CO2·m-2·s-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season.

본 연구는 헛개나무 용기묘를 생산하기 위한 적정 차광수준을 밝히고자 수행되었다. 헛개나무 1년생 용기묘를 대상으로 4가지 차광처리(0%, 35%, 55%, 75%)에 따른 용기묘의 간장, 근원경, 건중량, 묘목품질지수, 엽록소 함량 등의 반응 특성을 조사하였다. 연구 결과, 헛개나무 용기묘의 근원경, 잎 건중량, 뿌리 건중량은 차광처리구 간에 통계적인 유의성이 나타나지 않았으나(p≥0.05), 간장, 줄기 건중량, 엽록소 함량은 차광처리에 의해 55%와 75%의 차광처리에서 유의적인 차이를 나타내며 증가하였다(p<0.001). 간장과 줄기 건중량의 증가로 헛개나무 용기묘의 H/D율과 T/R율은 증가하였으며 묘목품질지수는 낮게 나타났다. 따라서, 헛개나무 1년생 용기묘 생산을 위한 적절한 차광수준은 묘목품질지수가 가장 높게 나타난 35%로 판단된다. 본 연구결과는 우량한 헛개나무 용기묘 생산을 위한 기초자료로 활용될 수 있을 것으로 기대된다.

The purpose of this study was to verify the sensitive areas when the AI determines osteoporosis for the entire area of the panoramic radiograph. Panoramic radiographs of a total of 1,156 female patients(average age of 49.0±24.0 years) were used for this study. The panoramic radiographs were diagnosed as osteoporosis and the normal by Oral and Maxillofacial Radiology specialists. The VGG16 deep learning convolutional neural network(CNN) model was used to determine osteoporosis and the normal from testing 72 osteoporosis(average age of 73.7±8.0 years) and 93 normal(average age of 26.4±5.1 years). VGG16 conducted a gradient-weighted class activation mapping(Grad-CAM) visualization to indicate sensitive areas when determining osteoporosis. The accuracy of CNN in determining osteoporosis was 100%. Heatmap image from 72 panoamic radiographs of osteoporosis revealed that CNN was sensitive to the cervical vertebral in 70.8%(51/72), the cortical bone of the lower mandible in 72.2%(52/72), the cranial base area in 30.6%(22/72), the cancellous bone of the mandible in 33.3%(24/72), the cancellous bone of the maxilla in 20.8%(15/72), the zygoma in 8.3%(6/72), and the dental area in 5.6%(4/72). Consideration: it was found that the cervical vertebral area and the cortical bone of the lower mandible were sensitive areas when CNN determines osteoporosis in the entire area of panoramic radiographs.

목적 : 본 연구는 노인의 역할 수행을 위한 역할 수행 작업치료를 중재, 역할 유형, 중재의 결과를 나타내 기 위한 종속 변수와 평가도구, 중재 효과에 대해 알아보고자 하였다. 연구방법 : 문헌 검색은 국내와 국외 데이터베이스인 학술연구정보서비스(Research Information Sharing; RISS), Pubmed, Cumulative Index of Nursing and Allied Health Literature(CINAHL), Excerpta Medica Database(EMBASE), SCOPUS, Web of Science를 이용하였다. 문헌 선택은 Preferred Reporting Items for Systematic Reviews(PRISMA) 흐름도에 따라 진행하였으며, 최종 17편의 문헌을 선정하였다. 최종 선정한 문헌을 근거기반 연구의 질적 수준모델과 Critical Appraisal Skills Programme(CASP) randomized controlled trial checklist, CASP case control checklist, CASP qualitative checklist를 사용 하여 근거수준과 적용된 연구방법의 질적 평가를 하였다. 결과 : 최종 17편의 문헌 중 Ⅰ단계에 해당하는 무작위 대조연구는 6편으로 나타났으며, 6편의 연구가 CASP checklist의 모든 항목을 충족하였다. 작업치료 중재는 자기관리자의 역할에 대한 중재가 13개로 가장 많았다. 여가 참여자 역할과 조직구성원 역할 각각 9편, 친구 역할 7편 순으로 나타났다. 중재 결 과를 도출하기 위한 종속변의 평가도구는 정신적 건강이 8개로 가장 많았고, 다음으로 신체적 건강, 삶 의 질 평가가 각 6개로 나타났다. 결론 : 본 연구를 통해 노인의 역할 수행을 위한 작업치료 중재를 알아보았으며, 향후 노인의 역할 수행을 위한 작업치료 중재 전략과 목표를 설정할 수 있는 근거를 제시하였다. 향후 고찰 연구에는 생애주기에 따른 노인이 아닌 다른 역할 평가 및 역할 수행이 필요한 대상자 포함이 필요하며, 국내 실정에 맞는 역 할 수행 작업치료 중재에 대한 연구가 필요하다.

E171, a mixture of titanium dioxide, has been widely used as a food additive due to its whitening effect and low toxicity. However, it has been proven that E171 is no longer safe for public health. So far, there are insufficient studies on the toxic effects of E171 on organisms especially using standardized test methods. In this study, toxicity assessments of E171 to two aquatic species, water flea (Daphnia magna) and zebrafish (Danio rerio), were performed using modified standardized test methods based on the physicochemical properties of E171. The hydrodynamic diameter, polydispersity index, and turbiscan stability index (TSI) were measured to ensure the dispersion stability of E171 in exposure media during the test period. The EC50 for immobilization of water flea was 141.7 mg L-1 while zebrafish was not affected until 100 mg L-1 of E171. Measurements of reactive oxygen species (ROS) and antioxidant enzyme activities confirmed that E171 induced oxidative stress, leading to the activation of superoxide dismutase and catalase in both water flea and zebrafish, although the expression of antioxidant enzyme genes differed between species. These results suggested the potential risk of E171 to aquatic organisms and provided toxicological insights into the impacts of E171 on the environment.

This study aims to promote the necessity for future research from the perspective of the indoor environment by analyzing the trend of domestic COVID-19 confirmed cases and examining previous quantitative research cases. The limitations of the qualitative management plan were identified by analyzing the trend of confirmed cases and deaths in Korea and the trend before and after the implementation of 'social distancing' and 'step-by-step daily recovery', which are actually being applied. The trend of initial and follow-up measures for the outbreak of infectious diseases in Korea is judged to be a case where the importance of qualitative and quantitative management should be confirmed to prepare absolute quarantine measures. The results suggest that examining previous research cases and preparing through advanced research for effective application.