vThis study analyzed the coat color-related genes of MC1R, ASIP, ECA3-inversion, and STX17 of 1,462 Jeju horses administered by the Jeju Special Self-Governing Province. This was done to investigate the distributional characteristics of coat color-related genes in the Jeju horse group and the changes of its coat color-related genes by generation. The genotype frequency of the MC1R gene of E+/E+ and E+/Ee related to black coat color was 0.122 and 0.447, respectively, while Ee/Ee of the chestnut genotype was 0.429. The genotype frequency of the ASIP gene of AA/AA, AA/Aa, and Aa/Aa was 0.46, 0.448, and 0.091, respectively, where the genotype frequency of Aa/Aa turned out to be relatively low. The To/To and +/To genotype that manifests the Tobiano shape was 0.001 and 0.119, respectively, with the share of Tobiano shape around 12%. The genotype frequency of G/G and G/g of STX17 related to grey coat color was 0.002 and 0.680, respectively, with the share of grey horses among the Jeju horse group at 68.2%. As for the change of coat color genes by generation, no large changes were observed in the MC1R and ASIP genes. In ECA3-inversion, the To allele that manifests Tobiano significantly decreased following the generational change (p<0.05), while the STX17 G allele related to grey coat color significantly increased following the generational change (p<0.05). It will be necessary to examine the coat color genes when selecting breeding horses so that the diversity of coat colors among the Jeju horse group can be maintained.

본 연구는 오차드그라스와 톨 페스큐 혼파초지에서 비축 시기별(8월 1일, 15일, 30일) 으로 처리하여 11월 중순경(11.10∼11.12)에 수확할 때 목초의 비축시기에 따른 생산량과 목초의 사료 가치 등을 비교하기 위해 수행하였다. 목초 비축시기에 따른 오차드그라스와 톨 페스큐 혼파초지(동량 혼파초지)의 건물수량은 8월하순구가 2,983.0kg/ha으로 가장 높은 경향을 보였다. 목초의 비축시기가 늦어짐에 따라 목초의 생산량도 증가하였으나 유의성이 인정되지는 않았다. 조사료의 조단백질은 16.5∼18.16%으로 목초 비축시기별로 일정한 경향을 나타내지 않았다. NDF와 TDN는 비축시기가 늦을수록 증가하는 경향을 나타내었으나 유의성이 인정되지는 않았다. 반면에 ADF 비율은 목초 비축시기가 감소함에 따라 점차 감소하는 것으로 나타내었으나 유의성이 인정되지는 않았다. 무기물의 변화는 비축 기간이 증가할수록 P, Ca, Mg, Na, Zn이 증가하였고 반면에 Mn, Cu는 감소하는 경향을 나타내었다. 결과적으로 8월 초순부터 초지를 비축할 경우 고온으로 인한 생육 위축으로 목초의 수량증가는 기대하기 어렵다. 따라서 제주도에서 가을철에 활용하기 위한 초지를 비축할 경우에는 8월 하순 이후에 시작하는 것이 바람직할 것으로 사료된다.

This study was conducted to evaluate the chemical composition and physico-chemical meat quality properties for Jeju-horse (Jeju-horse×Thoroughbred) with different fattening periods (4-, 8- and 13.5-month). In chemical composition, the moisture contents were decreased as the fattening periods increased. The fat contents were 3.78% at 4-months and they were decreased such as 70∼76% at 13.5-months whereas the protein contents increased as the fattening period increased. The cooking loss was highest (33.41%) at 4-months group and decreased after that periods, however, there was no significant difference among 3 fattening period groups. The Warner-Bratzler shear force (WBS) values were lowest at 4-months group and tended to increase as the fattening period increased. In mineral contents, the contents of Fe, Na, Zn were significantly higher for 4-month group than 8- and 13.5-month group whereas the contents of Mg were significantly lower for 4-month group than 8- and 13.5-month group (p＜0.05). The results of the amino acid composition analysis showed that cystein, methionine, threonine, serine, glutamic acid, alanine, valine, lysine, histidine, arginine contents were significantly increased and tyrosine contents were significantly decreased as the fattening period increased (p<0.05). The contents of palmitic acid (C16:0) were highest at 4-month group and they decreased as the fattening period increased (p＜0.05). The contents of oleic acid (C18:ln9) were highest at 8-month group and they were lowest at 13.5-month group. The total contents of unsaturated fatty acids were significantly higher for 13.5-month group than those for 4-month groups (p＜0.05). In conclusions, the fat contents were decreased whereas the protein, Fe, Mg contents and WHC increased as the fattening periods increased. Overall contents of amino acids increased only except several amino acids. The total contents of unsaturated fatty acids were increased as the fattening period increased, however they were not significantly different when those were fattened more than 8-month. These results indicated that longer fattening period could be more effective to enhance horse meat quality.

Raising replacement heifers is important aspect of dairy farm management that can be characterized as a long-term performance and high-cost production period. This study was investigated the effect of month of age at the first pregnancy on calving difficulty of Holstein heifers. Heifers was inseminated with semen of easy-calving sire and the reproductive performance was compared for the effect of first pregnancy at 12-14 months of age (12 month old), 15-16 months of age (15 month old), 17-19 months of age (17 month old) and > 20 months of age (20 month old) in Holstein heifers. Holstein heifers showed different easy calving score (ECS) depending on their ages; frequency of ECS 1 was 55.2%, 64.9%, 59.4% and 52.0% at 12, 15, 17 and 20 month of age, respectively. Frequency of ECS 4 was 5.2%, 1.8%, 6.3% and 4.8% at 12, 15, 17 and 20 month of age, respectively. The rates of retained placenta postparturition in first pregnancy age category was 12.3% at 12 months of age, 5.3% at 15 months of age, 9.4% at 17 months of age and 19.0% at 20 months of age. When It was evaluated by present results, the reproductive performance like easy calving score (ECS), retained placenta rates, and abortion and stillbirth rates of heifers which was inseminated at 14 months old or less and at 17 months old or more was reduced. Therefore, the first pregnancy age for optimum reproductive performance was 15-16 months.

Horses are non-ruminant and monogastric animal. If concentrates are fed plentifully at a time, undigested starch reaches large intestine and it activates amyloytic bacteria. Finally, it lowers a pH level and causes colic. Therefore, standard feed of concentrates and period of feed are important factors to fat up horses. This study was conducted to evaluate the productivity and carcass quality, quantity grade for Jeju horse (24 month old) with different fattening periods (4-, 8- and 13.5-month). The body weights were increased as the fattening periods increased and the increase rate was highest at 4-month group. The daily weight gain was decreased as the fattening periods increased such as 0.46 kg for 4-month group, 0.39 kg for 8-month group and 0.34 kg for 13.5-month groups. The daily intake was increased as the fattening periods increased such as 4.42 kg for 4-month group, 5.00 kg for 8-month group and 5.26 kg for 13.5-month groups. The daily feed conversion was increased as the fattening periods increased such as 11.71% for 4-month group, 17.69% for 8-month group and 18.53% for 13.5-month group. The backfat thicknesses and loin-eye areas were 3.50 mm, 83.50 ㎠ and increased to 4.20 mm, 94.00 ㎠ until they were fattening up for 8-month then they were decreased to 4.00 mm, 89.40 ㎠ when they were fattening up to 13.5-month. There was no significant difference in the meat color among 3 fattening groups, however, the a-values (redness) of meat color and b-values of fat color were highest (5.0 and 3.80, respectively) for 13.5-month group. The texture was not significantly different among 3 fattening groups. The maturity levels were in the 3.8∼4.5. All horses used in the experiment had the quality grade 2 and the horses for 13.5-month group had the quantity grade A, whereas the horses for 4-month group had the quantity grade B or C. Jeju horse had the low quality grade (quality grade 2) although the carcass yield increased when they were fattened from 24 month to 37.5 month.

Presently, the first pregnancy age of dairy heifers attended National Dairy Herd Improvement Program (NDHI) in Korea has increased, and more research have been requested to evaluate the possibility of decreasing the first pregnancy age of dairy heifers. Accordingly, this study was investigated the effects of the month of age at the first service and pregnancy on the reproductive efficiency of Holstein heifers. For these, the reproductive performance of heifers was analyzed by evaluating the effects of first insemination service at 12-14 months of age (12 month old), 15-16 months of age (15 month old), 17-19 months of age (17 month old), and > 20 months of age (20 month old) in Holstein heifers. The service numbers per conception in the first insemination months were 1.85±1.22, 1.59±0.77, 1.58±0.72, and 1.76±0.97 times at 12, 15, 17 and 20 months of age, respectively. The conception rates in the first service were 51.2, 57.8, 54.8, and 52.9% at 12, 15, 17 and 20 months of age, respectively. The conception ages were 453.2±78.9, 512.1±75.9, 590.1±98.0, and 713.0±74.5 days in the heifers serviced firstly at 12, 15, 17 and 20 months of age, respectively. There was significantly difference in the conception age among groups (p<0.05). The cumulative conception rates in the heifers serviced firstly at 12, 15, 17 and 20 months of age were 51.21, 57.8, 54.8 and 53.0% by the first service, 29.8, 30.1, 32.3, 23.5% by the second service, and 19.0, 12.1, 12.9 and 23.5% by the third service, respectively. The service numbers per conception in the first pregnancy months were 1.31, 1.55, 2.0, 2.05 times at 12, 15, 17 and 20 months of the first service age, respectively. The service numbers per conception in the 12 and 15 months of age were significantly lower than those in the 17 and 20 months of age (p<0.05). The rates of repeat-breeder were 6.0, 11.3, 22.0, and 41.0% in the heifers serviced firstly at 12, 15, 17 and 20 months of age, respectively. These results demonstrate that first pregnancy of Holstein heifers should be conducted at 15 months of age, because the age at first pregnancy in heifers can be substantially influenced through implementation of reproductive management and performance.

제주마 경주 능력 개량을 위한 기초 자료 확보를 위하여 혈통 정보량과 오류량이 유전 모수 추정에 미치는 영향을 분석하였다. 혈통 자료는 램덤으로 혈통 정보의 삭제(0~25%) 또는 오류 유발(0~25%)과 각 수준별 20회 반복으로 모의자료를 형성하였다. 분석에 이용된 형질은 제주마 1,000m 경주의 주파 시간을 이용하였고, 상가적 유전 분산의 추정은 DF-REMLAI 알고리즘을 이용하였다. 개체의 양부모에 대한 정보 유실량과 오류량의 증가는 선형적인 회귀관계로 상가적 유전 분산 성분(유실 b = -0.079, 오류 b= -0.114)과 유전력 추정치(유실, b= -0.006; 오류, b = 0.008)의 감소를 초래했다(p＜0.01). 특히 개체의 양부모에 대한 정보유실보다는 정보 오류가 상가적 유전분산량과 유전력 추정치를 보다 크게 왜곡하는 것으로 나타났다. 부 또는 모에 대한 정보의 유실과 오류의 증가도 대부분 오차 분산 성분과 영구 환경 분산 성분의 증가를 유발시키고, 상가적 유전 분산성분의 감소를 가져왔다. 원자료에서 얻은 모수를 기준으로 했을 때 양부모에 대한 정보의 유실량과 오류량이 1% 증가함에 따라 유전력은 각각 0.92% 및 1.39%씩 낮아지는 관계를 보였다 (p＜0.01). 제주마의 1,000m 주파 속도에 대한 유전력은 0.60으로 추정되었지만, 초기 집단에 존재하는 혈통 정보의 누락 및 오류를 고려한다면 실제보다도 10% 정도는 낮게 추정되고 있다고 사료되었다. 이와 같은 결과는 상가적 유전 분산량의 감소는 물론, 선발에 따른 제주마의 경주 능력에 대한 유전적 개량량에도 영향을 줄 것으로 판단된다.

A study was performed to investigate the effect of reseeding rates of Italian ryegrass (IRG)〔Lolium multiflorum L.〕on its nutritive value and productivity in native grasssland of Jeju region from Oct. 2011 to Jul. 2012. IRG was sown at reseeding rates (0 30, 40 and 50 kg/ha) in native grassland. When IRG was sown, there was no draught and lodging damage. Plant height in treatment 30 kg/ha of reseeding IRG was the tallest at all harvest stages (p<0.05). DM yield in treatment of 30 kg/ha of reseeding IRG was 12,790 kg/ha and increased 2.7 folds as compared to control. However, there was no significant difference among the treatments. The content of neutral detergent fiber (NDF) significantly decreased in treatment of reseeding, compared to control (p<0.05). In general, the content of NDF in second harvest stage was lower than that of control in first harvest stage. The content of acid detergent fiber (ADF) also showed simliar trend as content of NDF. The content of total digestible nutrients (TDN) in treatments of 30 kg/ha and 50 kg/ha of reseeding were approximately 45.2% each. The content of TDN in treatments of 30 kg/ha and 50kg/ha of reseeding increased as compared to 40 kg/ha and control of reseeding (p<0.05). Therefore, it is conclued that reseeding rate of IRG may improve the yield and nutritive values of IRG at 30 kg/ha.

While Ardisia crenata Sims. recently has been used increasingly as an ornamental plant, It is endangered in natural forest. The optimum treatments of the various concentrations priming agents to improve seed viability and germination of A. crenata (ornamental plant) were also estimated. Germinability was significantly when the seeds of A. crenata was soaked in PEG solution at 15oC for 4 days, the optimum treatment for improving germination of A. crenata was observed when the tested seeds was soaked in −0.5 MPa of PEG solution in 92%. Overall results would be useful means for propagation and production of A. crenata.

상표법 제73조 제1항 제3호에서 규정하는 불사 용으로 인한 취소심판이 청구된 때에는 상표권자 가 그 취소심판청구일로부터 3년 이내에 등록상표 를 지정상품에 사용한 사실을 입증하여야 하는데, 불사용으로 인한 취소심판에 있어서 등록상표와 실사용상표 간의 동일성 판단이 쟁점이 된다. 판례 에서는 동일성이라는 개념을 도입하여 등록상표 그 자체뿐만 아니라 거래사회통념상 등록상표와 동일하게 볼 수 있는 형태의 상표를 포함한다고 분 명히 밝히고 있다. 이러한 원칙에 입각하여, 등록상표가 결합상표 이고 결합상표를 이루는 기호나 문자 또는 도형들 이 각기 상표의 요부를 구성하고 있는 경우에는 그 중 어느 한부분만을 상표로 사용하였다 하더라도 이를 두고 등록상표를 정당하게 사용한 것이라고 는 할 수 없다고 한다. 따라서, 등록상표를 구성하 는 도형의 경우에는 그 식별력의 정도가 높고 낮음 에 크게 상관없이 이를 누락, 생략 또는 삭제하여 사용한 때에는 등록상표의 동일성 범위 내의 사용 으로 보기 어렵다는 것이 원칙이나, 등록상표를 구 성하는 문자의 경우에는 해당 문자가 식별력이 극 히 약한 정도에 그친다면 이를 누락, 생략 또는 삭 제하여 사용한 때에는 등록상표의 동일성 범주 내 의 사용으로 인정하고, 식별력이 상표등록을 허여 받을 수 있는 정도의 높은 경우라면 이를 누락, 생 략 또는 삭제하여 사용한 때에는 그러하지 아니하 다고 한다. 그런데, 등록상표를 구성하는 문자부분 간에 동 일한 칭호 또는 관념을 지니지만 이를 한글, 영문 자, 한문 또는 일본어 등 다양한 문자로 표기하여 병기한 등록상표를 어느 한 문자만으로 사용한 때 또는 이들 문자 중 하나의 문자만으로 구성된 등록 상표를 각기 다른 다양한 문자로 표기하여 사용한 때에, 거래사회통념상 등록상표와 동일하게 볼 수 있는 형태의 상표의 사용이 아니라는 취지로 일관 되게 판시하여 왔으나, 최근 2013년 대법원 전원합 의체 판결을 통해 문자만으로 결합된 등록상표의 동일성 범위에 대한 판단 기준을 변경하였다. 그러 나, 영문자 외에 제2 외국문자로의 다양한 문자를 병기하거나 상호 변형하여 사용되는 경우에는 거 􀓈명지특허법률사무소변리사 래사회통념상등록상표와동일하게볼수있는형태의 사용인지 등에 관하여 여전히 의문이 남는다. 등록상표와 실사용상표 간의 동일성에 관한 판단 기준은 대법원 판례를 통하여 오랫동안 정립되어 그 기준이 명확히 하고 있긴 하나, 한국상표법에도 외국의 입법례 특히 일본의 입법례와 마찬가지로 상표의 변형사용을 어느 정도 인정해주는 입법적 보완이 필요하다.

This experiment was conducted to verify the effect of spraying ethanol extracts (green tea and wormwood) on pH, TBARS, VBN, and total microbes of chicken meat during storage. The ethanol extracts of green tea and wormwood were sprayed on the chicken carcasses (0.2 and 0.2 g extract/㎏meat, respectively). Treated sample showed higher lightness (L* values) and yellowness (b* values) and lower redness (a* values) than those of control. The TBARS values were significantly lower for the treatments than the control on storage day 4, especially, the treatment of the wormwood extracts had lowest TBARS value. The VBN values were relatively lower values for the treatments than the control on storage day 4, especially, the treatment of the wormwood extracts had lowest VBN value on storage day 6. Wormwood samples showed lower total aerobic and E. coli counts than the others on day 6. Therefore, the spray with natural wormwood extracts could be useful to extend the shelf-life by preventing lipid oxidation and reducing the microbial growth during storage of chicken meat.

The objective of the study is to assess temperament of a horse based on general temperament test by a questionnaire survey. Five test criteria were identified: gentleness, patience, aggressiveness, sensitivity, and friendliness, each on a 5-point scale. 114 horses bred at the National Institute of Animal Science (NIAS) in 2010, 2011 and 2012. The horses recorded scores of 3.6∼3.9 for gentleness, 3.1∼3.6 for patience, 3.4∼4.0 for aggressiveness, 2.8∼3.2 for sensitivity, and 3.4∼3.8 for friendliness, the overall score for sensitivity the lowest. Horses born in 2012 scored lower than the rest in all five areas at a statistically significant level (P<0.05). By gender, the colts scored higher than the fillies in all five areas, but the discrepancy was not statistically significant. Factor analysis yielded only one factor, and the Cronbach's α value was 0.980 for standardization of Factor 1, indicating a high reliability of internal consistency. The correlation coefficients among the test criteria ranged between 0.85 and 0.91 (P<0.01). The assessment criteria used in this study are expected to provide a useful basis designing a temperament test horses.

This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×108/ml by doubling in every 30 minutes at 4℃ cold chamber. The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm for 10 minutes. The height and duration affect the freezing speed by temperature. The frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. Frozen-thawed sperm were evaluated sperm viability, membrane integrity and acrosome integrity. Post-thawed sperm viability has been significantly higher (p<0.05) in fresh sperm (93.27±1.62%) than frozen-thawed sperm (73.34±3.27%). However, there were no significant differences between fresh and frozen-thawed dead cell rate (7.35±2.63 vs, 13.71±2.85). In sperm motility, between Triladyl and AndroMed Extender, there was no significant different (72.86±2.83 vs, 81.47±2.48), similarly, the dead cell rates was similar (18.41±3.42% and 17.26±4.25). The results of our study suggest that AndroMed to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Jeju Native Black bull semen.

This study was conducted to investigate the effects of different levels of soybean curd residue as an ingredient of TMR on growth performance and carcass characteristics of Holstein steers. Twenty four Holstein steers of 7 months age(average body weight 274.6 kg) were randomly allocated to 3 experimental groups, which contained 8animals per group during 15-months feeding trial. The treatments composed of the group fed concentrate and rice straw separately (Control), the group fed TMR containing low levels of soybean curd (T1), the group fed TMR containing high levels of soybean curd residue (T2). Body weight and average daily gain were not significantly different among the treatment groups during the feeding trial periods. Feed intake was not differ among treatment groups, but feed conversion ratio was significantly higher in T2 group compared to T1 group during late fattening stage (p<0.05). However, it was not different in overall average. According to the yield traits, carcass weight of control, T1 and T2 were 466.4, 451.1 and 460.1 kg, respectively. Back fat thickness, rib eye area and yield index were not different among the groups. The marbling score, meat color, texture and maturity in quality traits were not different among the treatment groups, but fat color was significantly higher in T2 group compared to T1 group (p<0.05). The quality grade (1+:1:2:3, NO.) tended to be higher for T1(0:2:1:5) and T2 (0:1:4:3) compared with the control (0:0:3:5). Therefore, these results indicated that TMR with soybean curd residue may be appropriate the growth performance and carcass characteristics of Holstein steers.

자생지별 꼬리진달래의 생리반응을 측정한 결과 광보상점은 석포리 11.8 μmol m-2 s-1, 연하리 11.5 μmol m-2 s-1, 월악산 10.4 μmol m-2 s-1로 나타났으며, 광포화점은 500~600 μmol m-2 s-1 범위로 나타나 음수의 생리적 특성을 갖고 있었다. 광포화시의 광합성 능력은 석포리 5.5 μmol m-2 s-1, 연하리 5.4 μmol m-2 s-1, 월악산 5.6 μmol m-2 s-1로 나타나 광보상점과 광포화점, 광합성 능력은 자생지별로 유의적인 차이가 나타나지 않았다. 온도 변화에 따른 자생지별 꼬리진달래의 순광합성 속도는 3지역 모두 20~30℃ 온도역에서 높은 광합성 속도를 유지하는 것으로 나타났으며, 그 중 25℃에서 가장 활발한 광합성을 하는 것으로 나타났다. 연하리의 수분이용효율이 석포리와 월악산에 비해더 높은 것은 포화광도에서 낮은 기공증산속도로 인한 결과였으며, 꼬리진달래의 자생지별 엽록소 a, b 및 총 엽록소 함량은 유의적차이가 없는 것으로 나타났다. 위의 결과를 통해 꼬리진달래는 음수의 특성을 가지며, 생육에 적합한 온도는 20~30℃인 것을 알 수 있었다.

본 시험은 버뮤다초지에서 이탈리안 라이그라스 파종시기가 목초생산성 및 식생변화에 대해 조사하기 위하여 2009년 9월부터 2010년 10월까지 해발 200 m에 위치한 제주도 제주시 오등동에 위치한 국립축산과학원 난지축산시험장 방목초지(해발 200 m)에서 수행되었다. 남방형 목초인 버뮤다그라스 초지에서 시험구를 난괴법 3반복으로 배치하였다. 이탈리안 라이그라스는 파종전 버뮤다그라스 목초를 초장이 2~3 cm 정도 높이로 예취를 한 후 파종을 하였다.

본 연구는 제주조릿대의 성장단계별 영양소 함량을 분석하고 말에서의 소화율을 검정하여 말 사료자원으로써의 활용가치를 평가하고, 제 주조릿대 군락지 내 말 방목에 따른 제주조릿대의 생산성, 생육특성, 주변 식생의 변화 등을 구명하기 위해 수행되었다. 제주조릿대를 구초 (1년생)와 신초 (당년생)의 성장단계로 분류하여 조단백질, NDF, ADF 및 무기물 함량을 분석했다. 제주마 5두를 이용 제주조릿대 구초와 신초의 전분채취법을 이용한 외관상 소화율을 검정했