시설재배 딸기를 위한 생력적이고 환경친화적인 순환식 수경재배기술을 확립하기 위하여 고형배지 종류별로 딸기의 무기이온 흡수 특성을 조사하였다. 톱밥배지를 제외한 나머지 세 종류의 배지에서는 대체적으로 많은 양의 질산태질소의 흡수를 나타내었는데, 펄라이트배지와 입상양면배지 보다는 코코피트배지에서 약간 높은 흡수농도를 나타내었다. 인은 톱밥배지를 제외한 세 종류의 배지에서는 초기에는 5me·L-1의 높은 흡수농도를 나타내었으며 생육이 진행됨에 따라 점차 감소하여 3me·L-1 정도의 흡수량을 나타내었는데, 이것은 비교적 높은 농도로서 추후 검토를 할 필요가 있을 것으로 생각되었다. 그러나 톱밥배지에서는 3월 중순까지 인의 흡수가 거의 없는 것으로 나타났다. 칼륨의 흡수농도는 톱밥배지를 제외하고는 세 배지 모두 비슷한 흡수량을 나타내었는데 생육초기에는 비교적 높은 흡수량을 나타내었지만 점차적으로 감소하여 4me·L-1 전후의 흡수량을 나타내었다. 칼슘의 흡수량은 펄라이트배지와 입상암면배지에서는 생육초기에 약간 높은 농도를 나타내기는 하였지만 생육전반에 걸쳐서 2me·L-1 정도의 안정적인 흡수양상을 나타내었다. 마그네슘은 코코피트배지에서 생육 전반기에 3me·L-1 정도의 높은 흡수농도를 나타내었으며 생육 후반기까지도 다른 배지에 비해서 가장 높은 흡수 농도를 나타내었다. 다음으로는 펄라이트 배지와 입상암면배지 순이었으며 세 배지 모두 마그네슘의 흡수는 대체적으로 1me·L-1 정도의 농도를 나타내었다. 본 실험의 결과는 고형배지를 이용한 순환식 딸기 수경재배에서 유용하게 활용될 것으로 생각된다.

This research was carried out as a survey on the contents of Lead (Pb) and Cadmium (Cd) in 5 type agricultural products unestablished safety guideline in Korea. The average levels of Pb: onion 0.010 mg/kg, cucumber 0.018 mg/kg, garlic 0.012 mg/kg, green pepper 0.027 mg/kg, sesame 0.029 mg/kg. The average levels of Cd: onion 0.006 mg/kg, cucumber 0.002 mg/kg, garlic 0.008 mg/kg, green pepper 0.011 mg/kg, sesame 0.024 mg/kg. The present result of this study showed that Pb and Cd contents in the whole samples were less than the maximum residual levels of the codex standard. The levels of exposure assessment for Pb and Cd by intake from vegetables and sesame were merely at 1.3 × 10−⁴(mean) ~5.1 × 10−⁴(95th percentiles) μg/kg bw/day for Pb, 7.5 × 10−5(mean)~3.6 × 10−⁴(95th percentiles) μg/kg bw/day for Cd. The data from this research will be valuable source for database construction for science-based safety control and management for the trace metal contamination in food including agricultural products.

β-Glucosidase 효소활성이 높은 균주를 선발하기 위하여 다양한 김치에서 분리된 젖산균의 β-glucosidase 활성을 탐색하였다. 김치에서 분리된 156개의 젖산균 중 134개의 균주만이 cellobiose를 탄소원으로 대사하였으며, 세포내 β-glucosidase 활성이 세포외 활성보다 현저히 높았다. 배추김치에서 분리된 W. cibaria KFRI88010 균주가 3.7±0.5 unit/mg protein으로서 가장 높은 세포내 β-glucosidase 효소활성을 나타내었으며, 효소활성은 pH 5, 37oC 반응조건에서 가장 높게 나타났다. Mn2+를 비롯한 금속이온은 효소활성을 크게 저해하였다. W. cibaria KFRI88010 균주를 배양할 때 사용한 탄소원 중, fructose는 cellobiose나 glucose와 비교하여 약 2.5배 이상의 높은 세포내 β-glucosidase 효소활성을 나타내었다.

목적: 고속도로에서 고속 주행 시 부착위치가 상이한 네비게이션의 목적지 검색을 할 때 위험성을 정량적으로 측정하고자 하였다. 방법: 자동차 화상 시뮬레이터를 이용하여 평균연령이 23.143± 2.67세인 남자 대학생을 대상으로 네비게이션 검색 시 시선과 생리신호를 측정하고, NASA-TLX를 작성하도록 하였다. 결과: 하단에 부착한 네비게이션이 시선연구결과 유의하게 도로에서 시선을 떼는 현상을 나타났으며(p＜0.0１), NASA-TLX결과 역시 하단에 부착된 경우 각성수준(Workload)를 증가시켜 작업수행도를 저하를 가져올 수 있다. 결론: 고속 주행 중 네비게이션의 목적지 검색은 매우 위험하며 특히 하단에 부착된 네비게이션의 경우 매우 사고위험이 있다고 할 수 있다.

The pattern of wound healing process differs markedly according to the cell types. Gingival wounds heal more rapidly without scar, however dermal wounds show collagen laid down in thick disorganized patterns and keloid formation. This h as b een s uggested t o be d ue t o the presence of d ifferent E C M components a nd c ytokines a s well a s growth factors. The purpose of this study was to examine the differential expression of genes in connection with keloid formation in gingival fibroblasts (hGFs) and dermal fibroblasts (hDFs) in response to inflammation. In this study, we investigated the differences between hGFs and hDFs in the expression and production of cyclooxygenase (COX-2), prostaglandins E2 (PGE2), transforming growth factor (TGF)-β, collagens, matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs) which play important roles in collagen deposition in wound healing. The hGFs and hDFs were primary cultured and allocated to arachidonic acid (AA) treatment group and control group. Protein and mRNA were extracted right after (0 hr) and 24 hr after AA treatment. At a defined concentration of AA in hGFs and hDFs, MTT assay was performed. The mRNA and protein expression levels of COX-2, TGF-β, collagen 1 and 3, MMP 1 and TIMP 1 were examined by Real-time PCR and Western blots. The amounts of PGE2 were measured by enzyme-linked immunosorbent assay (ELISA).The expression of COX-2 and TGF-β exhibited reduced levels in hGFs , but were increased in hDFs at 24 hr after AA treatment. Production of PGE2 was increased in hGFs and hDFs at right after AA treatment but, not changed at 24 hr after AA treatment. The protein and mRNA expression of collagen 1 and 3 were decreased in hGFs , whereas increased in hDFs at 24 hr AA treatment. Expression of MMP-1 protein was increased in hGFs at 24 hr but, was decreased in hDFs at 24 hr compared with that of control. The protein expression of TIMP-1 was decreased in hGFs but, was increased in hDFs at 24 hr compared with that of control. These observations demonstrate differential expression between gingival and dermal fibroblasts in regulation of collagenolytic capacity by extracellular matrix-associated genes in keloid formation associated with wound repair.

In this study, odor active compounds, emitted from food waste composting facility, were analyzed and their contribution degree were evaluated. Sample gases were collected at the inlet and outlet of scrubbing tower and bio-filter, respectively. The odor active compounds were separated by GC/FID/Olfactometry (GC/FID/O) and identified by GC/MS. Odor intensity and SNIF (Surface of Nasal Impact frequency) were used to evaluate contribution of each odor active compound. It was possible to calculate contribution rate (%) and reduction rate (%) of odor active compounds using analysis and evaluation of contribution degree of odor active compounds. As a result, the compounds showing the high contribution degree were fatty acids, ketones at inlet and aldehydes, ketones at outlet. Deodoring equipment of food waste composting facility appeared effective for the reduction of aldehydes, ketones but appeared week for the reduction of aldehydes. Although scrubbing tower and bio-filter could reduce 7 times of odor level, the outlet gases had odor dillution ratio of 3000, which exceed the allowable levels of Korean guideline by 6 times.

Environmental Enrichment (EE) alone is not capable of enhancing the fine digit and the forelimb functions. Therefore, we applied modified constraint-induced movement therapy (mCIMT) under the influence of EE to assess its effect on promoting improved forelimb sensorimotor functions. Focal ischemic brain injury was produced in Sprague-Dawley rats (60 rats, 250±50 g) through middle cerebral artery occlusion (MCAO). Before MCAO induction, all rats were trained in modified limb placing tests and reaching tasks for 1 week. Then they were randomly divided into three groups: Group I: application of standard environment (SE) after MCAO induction (n=20), Group II: application of EE after MCAO induction (n=20), Group III: MCAO+EE, mCIMT and task-oriented training that was initiated at 10th day after MCAO induction (n=20). We also applied mCIMT (between 9 AM and 5 PM/daily) which included restraining the forelimb ipsilateral to the lesion using the 'Jones & Schallert' method. We assessed the change of modified limb placing, single pellet reaching test and the immunoreactivity of BDNF by immunohistochemistry (pre, 1st, 5th, 10th and 20th day). Group I showed no improved outcome, whereas group II and III significantly improved on the use of the forelimb and the immunoreactivity. The qualitative analysis of the skilled reaching test, of group III showed the greatest improvement in the fine digit and the forelimb function. These results suggest that EE combined with mCIMT is more functional in promoting enhanced fine digit and forelimb functional movements.

Volatile fatty acids (VFAs) are malodor compounds which are produced from the decomposition of animal or plant species. It is very difficult to detect VFAs due to their strong adsorptive properties. In this study we develop an analytical method using headspace-GC/FID with an alkali-impregnated filter sampling. The addition of NaCl and H₂S0₄ makes the salt-out and pH-lowering effect, respectively. The high boiling points (141~185℃) and vapor pressures require a high temperature and long heating time for the standard sample in vials to reach an equilibrium. The analytical response was highest when the absolute quantity in the sample was 5 mL in a 22 mL vial. The addition of NaCl for the salt-out effect can give a higher sensitiviry by a factor of 1.1~4.2 than that of Na₂SO₄. The mass amount of 4.6 g of NaCl can result in a higher sensitivity, which is higher than the supersaturated solubility of 4.2 g. The concentration of H₂SO₄ is as low as 2% (v/v). When the concentration range is 8.3 -562.1 ppb, a coefficient of R²~0.99 can be obtained for the five VFAs samples. The analytical errors in a reproducibility test are less than 10% and the detection limit is estimated to be 0.05~0.1 ppb. Our headspace-GC/FID analytical method can be utilized to effectively detect the five kinds of VFAs which shall be restricted in Republic of Korea from the year of 2010.

본 연구에서는 수정란 이식을 위해 선발된 대리모에 이식 전 백신의 투여 시기가 임신과 유산에 미치는 효과와, 임신한 대리모의 분만 예정일 전에 백신의 투여가 송아지의 질병과 생존에 미치는 효과를 검토하였다. 실험 1에서는 한우 체외 수정란의 이식에 선발된 대리모의 백신 투여 시기가 임신과 유산에 미치는 영향을 검토하였다. 이식 전 주째 백신 투여시 임신율이 31.5%로서 대조군 및 주 투여군의 42.4 및 45.9%에 비하여 유의하게 낮았다(p<0.05

An analytical method for trimethylamine in ambient air was developed, using headspace gas chromatography with flame ionization detection. Trimethylamine was collected on the acid filter which was impregnated sulfuric aicd in the 47 ㎜ diameter of glass fiber filter. Trimethylamine collected on the acid filter was regenerated in the headspace vial and introduced into the GC analytical column directly. Several parameters such as sample volume, equilibrium temperature and time, and slurry method of filter were optimized to provide maximum detection response. Resolution power also compared according to liquid phase of analytical column. The detection limit of method was 0.13 ppb with 50 L sampling volume. The developed acid filter method is easy to deal with the field sampling and the method was adopted as the standard method for odor analysis in Korea.

In this study, the TVOCs and odor active compounds emitted from 3 PVC and 5 natural wallpapers were analyzed. The emitted odor intensities and characteristics of the wallpapers were estimated using air dilution sensory and direct olfactory methods. The emissions of TVOCs were measured using the SPME-cryogenic emission test method, which was modified from the RAL-GZ 479 method, which is used in Germany to evaluate the quality of wallpapers. It was found that there was no significant correlation between the emissions of TVOCs and the odor concentration. However, a correlation (0.57) was found between the emitted VOCs and the odor concentration of the low molecular weight compounds, which have strong volatilities, ranging from n-C6 to n-C10. For all 8 wallpapers, vinegar like odor was commonly perceived, with acetic acid identified as the main odor active compound withinthe odor. Benzaldehyde and esters, such as ethyl acrylate and n-butyl acrylate, were identified as odor active compounds, which were expressed as having rubber like smells by the panelists. These results indicate that odor should be included as a domestic regulation for the pollutants emitted from building materials. The identification of odor active compounds emitted from building materials could be useful in the production of eco-environment products.

It has been reported that light-emitting diodes(LED) can be used in the treatment of oral diseases. Although bio-stimulatory effects of LED irradiation such as promotion of wound healing have been well known, there are few reports about molecular mechanisms associated with wound healing by LED irradiation. The purpose of the present study was to investigate the expression pattern of various extracellular matrix(ECM) molecules in relation to wound healing after LED irradiation on primary human gingival fibroblasts(hGFs) in vitro. The source of light for irradiation was a continuous-wave LED emitting at a wavelength of 635 nm, and manufactured that energy density was 5 mW/cm2 on sample surfaces. The hGFs were irradiated for 1 hour at 37℃ in 5% CO2 humidified chamber. Experimental samples were acquired at 0 (right after irradiation), 24 and 48 hour after irradiation. To investigate the molecular mechanisms associated with wound healing, we examined the mRNA expression of 6 types of collagens, 7 types of matrix metalloproteinases(MMPs) and 4 types of tissue inhibition of metalloproteinases(TIMPs) after LED irradiation by RT-PCR. The mRNA expression of collagen 4, MMP-3, 9, and 16, and TIMP-3 was influenced by LED irradiation. Generally, the collagen expression of the irradiation group was slightly increased, particularly collagen 4 was significantly increased at 0 hour. The expression of MMP-3 was increased at 0 and 24 hours and MMP-16 was increased at 24 hours, respectively. The expression of MMP-9 was decreased at 0 hour and increased at 24 and 48 hours. The mRNA expression of TIMP-3 was significantly decreased at 24 and 48 hours after irradiation. These results suggest that the altered expression of ECM molecules after LED irradiation may contribute to the accelerated wound healing.

In this study, alkali impregnated filter sampling method was considered for VFAs in ambient air. When KOH concentration was 0.5 N and sampling flow velocity was 5~10 L/min the sampling efficiency was higher than 90 percent. More than 3 minute shaking make the sampled filter to be suspended in the 22.3 mL Vial. Alkali impregnated filter sampling method showed more than 4 times higher response compared to alkali solution sampling method, when the sample was analysed by headspace-gas chromatography. Five kinds of volatile fatty acids (propionic acid, i-butyric acid, n-butyric acid, i-valeric acid, valeric acid) were detected within 1.0~9.8 ppbv in the cow shed by alkali impregnated filter method and headspace gas chromatography. The alkali impregnated filter method was the most suitable sampling method for ambient volatile fatty acids with headspace gas chromatography analysis.

본 연구는 체외성숙 배지에 첨가하는 PVP의 농도, EGF, cysteine 및 PVP의 단독 또는 혼합첨가가 한우 체외수정란의 체외발생에 미치는 영향을 검토하였다. 체외성숙 배지에 PVP의 첨가농도(0.1~3.0%)에 따른 분할율은 차이가 없었으나, 배반포 발달율은 0.5% PVP 첨가군이 가장 높았다(P<0.05). PVP, EGF 및 cysteine의 단독 및 혼합 첨가에 따른 분할율은 cysteine 단독첨가군이 높았으나(P<0.05), 배반포 발달율은 차이가 없었다. Inner cell mass 수는 대조군과 cysteine 첨가군이 PVP 첨가군에 비하여 유의하게 높았고(P<0.05), 총 세포수도 cysteine 첨가군에서 가장 높았다. 수정란이식 결과는 대조군, EGF, cysteine 및 EGF+cysteine 군의 임신율은 46.1~63.6%로서 비슷하였으나, PVP 첨가군은 10%로서 다른 군에 비하여 유의하게 낮았다(P<0.05). 본 연구 결과는 체외성숙 배지에 PVP의 첨가로 배 발생은 가능하지만, 세포의 품질에는 악영향을 미치는 것을 보여준다.

목적 : 본 연구의 목적은 휴대전화기 문자 쓰기 속도와 손의 민첩성과의 상관관계를 알아보기 위한 것이다. 연구방법 : 손에 손상을 받은 경험이 없는 47명의 인제대학교 학생을 대상으로 하였다. 설문 조사 후에 Nine Hole Pegboard Test(Smith & Nephew Rehabilitation Division version)와 대상자의 휴대전화기로 일정한 문장을 쓰는데 소요되는 시간을 측정하였다. 결과분석은 SPSS 10.0을 이용하였다. 연구 대상자의 일반적인 특성에 따른 문자쓰기 속도와 손의 민첩성은 t-검정과 ANOVA를 사용하여 분석하였다. 그리고 문자 쓰기 속도와 손의 민첩성에 대한 관계를 알아보기 위하여 스피어만 상관계수(Spearman correlation coefficient)를 사용하여 분석하였다. 결과 : 연령과 문자 쓰기 속도와의 관계는 유의한 차이(P<0.05)가 있었으며 연령이 낮을수록 문자 쓰기 속도가 더 빨랐다. 성별과 오른손 민첩성과의 관계는 유의한 차이(P<0.05)가 있었으며 여자가 남자 보다 민첩성이 더 높았다. 문자 쓰기 속도와 손의 민첩성은 관계가 없는 것으로 나타났다. 결론 : 손의 손상이 없는 성인에서 휴대전화기의 문자 쓰는 속도와 손의 민첩성에서 유의한 상관관계는 없었다. 그러나 연령에 따라 문자 쓰는 속도에 차이가 있었으며, 성별에 따라 오른손 민첩성에 차이가 있는 것으로 나타났다. 앞으로 본 연구를 바탕으로 기능적인 손 사용에 영향을 미치는 요소들을 알아보기 위한 연구와 손 기능에 문제를 가진 사람을 대상으로 문자 쓰기 활동이 손 기능 향상에 도움을 줄 수 있는 것인가에 대한 연구가 필요하리라 생각된다.