This study investigated the stability of ethanolic extract from fermented Cirsium setidens Nakai (FCSN) in order to develop functional materials during different storage conditions. We evaluated pectolinarin and pectolinarigenin contents, total phenol content and antioxidant activity (DPPH radical scavenging activity and FRAP assay) of ethanolic extract from FCSN obtained by bioconverison at various temperatures (4, 25 and 50℃) and pHs (4.0, 7.0 and 10.0). Our results show that the pectolinarin, pectolinarigenin, and total phenol contents in ethanolic extract from FCSN were decreased during the storage periods. Moreover, the DPPH radical scavenging activity did not significantly change at 4℃ and 25℃. Pectolinarin and pectolinarigenin contents, total phenol content and DPPH radical scavenging activity of ethanolic extract from FCSN at acidic pH (pH 4.0) and neutral pH (pH 7.0) were higher than those at the alkaline pH range. These results indicate that the optimum storage condition of the ethanolic extract from FCSN was 4℃ and pH 4.0~7.0 range.

상압 유전체 장벽 콜드 플라스마 처리(atmospheric dielectric barrier discharge-cold plasma treatment, DBD-CPT)를 이용한 살모넬라 저해 효과에 닭가슴살 큐브의 표면 특성이 미치는 영향과 살모넬라 저해를 위한 최적 DBD-CPT 조건 그리고 최적 조건의 DBD-CPT 가 닭가슴살 큐브의 품질 특성에 미치는 영향을 연구하였다. 분리 유청 단백(Whey protein isolate, WPI) 용액으로 코팅하거나 혹은 코팅하지 않은 닭가슴살 큐브(1.51.51.5 cm3, 3.8 g)를 준비하여 Salmonella (~7 log CFU/sample)를 접종하였다. 접종된 닭가슴살 큐브를 폴리 에틸렌 포장재 중심에 위치시킨 후 CP형성 전압(25-38.9 kV)과 처리 시간(2-5 min)에 대해 반응 표면 중심 합성법으로 설계한 처리 조건들로 DBD-CPT하였다. Salmonella 저해에 대한 최적 조건은 반응 표면 분석을 이용해 결정되었고 최적 조건의 DBD-CPT 가 닭가슴살 큐브의 품질 특성에 미치는 영향은 TBARS (thiobarbituric acid reactive substance values) 값과 색도(CIE L*a*b*), 그리고 닭고기 조직감의 연한 정도의 지표인 전단응력(Shear force)을 통해 관찰하였다. DBD-CPT 는 코팅한 닭가슴살에서 3.8 ± 0.3 log CFU/sample, 코팅하지 않은 닭가슴살에서 1.4 ± 0.4 log CFU/sample만큼의 Salmonella 저해를 보였고, 이를 통해 코팅으로 형성 된 평평한 표면이 DBD-CPT 의 Salmonella 저해 효과를 증진시켰음을 알 수 있었다. 그리고 반응표면 분석을 통해 결정된 Salmonella 저해에 대한 최적 CP 형성 전압과 처리 시간은 각각 38.9 kV와 3.6 min이었다. 최적 조건에서의 DBD-CPT 에 의해 Salmonella는 3.9 ± 0.4 log CFU/sample만큼 저해를 보였다. 한편 최적 조건에서의 DBD-CPT 는 TBARS 값, 색도, 그리고 전단 응력에 유의적인 영향을 주지 않았다. DBD-CPT 는 상업적 포장재에 포장되어 있는 조리 닭가슴살 큐브의 Salmonella 를 효과적으로 저해하였으며, 닭가슴살의 품질 특성에는 영향을 미치지 않았으므로 포장된 육류-즉석 섭취 식품의 효과적인 살균 방법으로 제안될 수 있다고 사료 되었다.

The purpose of this study was to investigate the quality characteristics of roasted peanuts and the antioxidative effect of peanut oil added with sesame oil. Saltiness of roasted peanut increased with increasing salt content. In terms of color value, L and b values decreased as increasing concentration increased, whereas a value increased with increasing salt content. Overall, sensory evaluations proved that roasted peanuts with 15% added salt was preferred over other samples. For peanut oil added with sesame oil, acid values increased during the storage period, whereas samples made with sesame oil had lower values than the control group. Peroxide values increased rapidly for 21 days and then decreased. The acid and peroxide values were lower in peanut oil added with 50% sesame oil compared to peanut oil added with 30%, 10%, and 70% sesame oil, as well as the control. The TBA values of peanut oil made with 50% and 30% sesame oil were lower than those of the control and 70% and 10% sesame oil. According to the Rancimat method, PS-50% (524 min) and PS-30% (453 min) demonstrated longer induction periods as compared to the control (280 min), PS-70% (445 min), and PS-10% (291 min) samples.

Sewerage age more rapidly than other facilities, leading to the destruction of the pipeline. Therefore, the replacement of old sewer pipes through long-term construction is costly. In order to solve this problem, this study investigated the ferronickel based sewer pipe with improved chemichal resistance and durability from concrete rigidity pipe. ABAQUS was used as the finite element analysis program and the load was calculated by the method given in the sewage facility standard. Therefore, the results of this study can be referenced in the design and construction of sewer pipes.

During a construction of a road, a temporary bridge is often connected to a existing bridge. In this case, a fatigue stability problem, which is not considered in the design of the bridge, can be occurred in the main girder of the existing bridge due to the vehicle load direction change. In this study, the fatigue stability of the main girder and cantilever slab of the bridge was tested with the allowable fatigue stress of the design specifications of the road bridge. The big stress change was occurred at 55m away from the support, and the middle point of the span. Furthermore, the excess of the allowable fatigue stress of the design specifications of the road bridge was confirmed at the cross section. Hence, it was concluded that the reinforcements for the cantilever slab and main girder of the bridge are required. The bridge after the reinforcement was tested for the fatigue stability, and it was confirmed that the bridge is safe.

안동⋅임하 유입지천의 저서성대형무척추동물의 군집안정성 분석을 위해 4개 지점(St.1, St.2, St.3, St.4) 선정 후 2016년 5월부터 9월까지 총 4회 조사를 실시하였다. 총 5문 8강 18목 56과 108종 53,368.8개체가 조사되었고, 안동호(St.1, St.2)는 총 4문 6강 14목 49과 82종 31,253.9개체, 임하호(St.3, St.4)는 총 5문 7강 17목 50과 83종 22,114.9개체 가 조사되었다. 전체 출현 분류군 중 하루살이목이 안동호 17종(20.7%), 임하호 16종(19.3%)으로 가장 많은 종수비율을 차지하였다. 출현 저서 동물을 대상으로 군집분석을 실시한 결과 안동호의 평균 우점도지수는(DI) 0.66 평균 다양도지 수(H') 2.39 평균 풍부도지수(R1) 2.47 평균 균등도지수(J') 0.55로 나타났다. 임하호의 평균 우점도지수는 0.59, 평균 다양도지수 2.69, 평균 풍부도지수 2.61, 평균 균등도지수 0.62로 나타났다. 군집안정성분석 결과 두 수계 모두‘I’그룹(저 항력↑, 회복력↑)에 속하는 종들의 출현율이 안동호 66.2%, 임하호 62.5%로 가장 높게 나타났다.

In this study, we investigated to the heat transfer performance of coating nano-structure with various shapes and patterns on the heat transfer surface. As a result of the measurement of the 3D nano shape, it was confirmed that the roughness generally increases when the adhesive is sprayed on the coating surface and finished durability experiment. In the case of TEOS adhesive, the roughness increased by 0.074 ㎛, 0.012 ㎛ and 0.015 ㎛, and the contact angle decreased 12.64°, 1.31°, 9.84° at the coating time of 120 seconds, 180 seconds and 240 seconds, respectively. In the case of PVA adhesive, the roughness increased by 0.069 ㎛, 0.056 ㎛ and 0.03 ㎛, and the contact angle decreased 2.85°, 4.82°, 6.96° at the coating time of 120 seconds, 180 seconds and 240 seconds, respectively. In the case of DGEBF adhesive, the roughness increased by 0.042 ㎛, 0.053 ㎛ and 0 ㎛, and the contact angle decreased 0.81° at the coating time of 120 seconds, increased 4.82°, 6.96° at the coating time of 180 seconds and 240 seconds, respectively. As a result, the durability tends to decrease as more nano-structures are deposited, and 3D nano shapes, contact angles and SEM photographs showed that the performance of the PVA adhesive was superior among the three adhesives.

Background: The multiple hop test is an active performance test that has been commonly used to assess individuals with functional ankle instability. Previous studies have suggested that insufficiency of dynamic postural stability and passive stability during dynamic activities can have an influence on performance in the multiple hop test. However, no study has investigated the effects of dynamic postural stability training and ankle bracing on multiple hop test performance in individuals with functional ankle instability. Objects: The purpose of this study was to compare the immediate effects of dynamic postural stability training versus ankle bracing in the performance of the multiple hop test for participants with functional ankle instability. Methods: Twenty-nine participants with functional ankle instability who scored below 24 in the Cumberland Ankle Instability Tool were selected. The participants were randomly divided into two groups: a dynamic postural stability training group (n1=14) and an ankle bracing control group (n2=15). The multiple hop tests were performed before and after applying each intervention. Dynamic postural stability training was performed using visual-feedback-based balance-training equipment; participants in this group were asked to perform a heel raise in a standing position while watching the centering of their forefoot pressure to prevent excessive ankle inversion. Ankle bracing was applied in the control group. Results: When comparing the pre- and post-intervention period for both groups, both methods significantly improved the results of the multiple hop test (p<.05). However, no significant differences were shown between the dynamic postural stability training and ankle bracing groups (p>.05). Conclusion: Both dynamic postural stability training and ankle bracing showed significant improvement (2.85 seconds and 2.05 seconds, respectively) in test performance. Further study is needed to determine the long-term effects of dynamic postural stability training and to determine whether insufficient dynamic postural stability is a causative factor for functional ankle instability.

This study was to develop the products that the Lamp and Ballast are combined for the purpose of easy installation to complement the difficulty of the installation process due to the structure of existing product which the lamp and the Ballast are separated, and that have 8-wavelengths dual-lamp structure such as solar, an advantage of more than 3 times longer life than fluorescent lamp and immediately lighting. This study developed the commercialized products that can prevent the environmental pollution caused by low efficiency and short life time of existing Lamps, and can replace the LED Lighting products which has high Glare index with high price, has also developed the variety of application for industrial, commercial, indoor and military. The applied product are Street lights, Security lights, Flood lights, Indoor lights and lights for fishing. This study solved the optimum distribution and placement of components considering the lightest weight first and thermal interference caused by combination of lamp and ballast by thermal release through applying the double bulkhead design of ballast box, and implemented the high efficiency Eco-friendly products with excellent visibility which can be applied and used for indoor and outdoor both place through high temperature and high humidity test, which has an advantage of 8-wavelengths of same spectrum of solar through the initial trial production.

Walking method based zero moment position algorithms that can guarantee the stability of the biped walking robot while walking, but it moves the legs for the stability of the walking in a way that is not related to energy conservation. Walking method using ZMP can cause low battery efficiency and load on leg joints. The walking method using the passive walking, which is a natural and efficient method of walking, can reduce the load on the joints of the robot by using the method without using the inertia that occurs when walking and reduced control elements and efficient use of battery. In this paper, a biped robot with an actuator based on the principle of passive dynamic walker mechanism is applied to a passive walking algorithm. In order to solve the problem of stabilization of the posture during walking, the posture was stabilized by using the swing motion of the arm. and the walking movement of the robot was observed using the AHRS sensor applied to the robot .It was confirmed that the posture can be stabilized based on measured values using AHRS.

For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without crossreaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.

We have investigated the washing method of as-synthesized CdSe/ZnS core/shell structure quantum dots (QDs) and the effective surface passivation method of the washed QDs using PMMA. The quantum yield (QY%) of assynthesized QDs decreases with time, from 79.3% to 21.1%, owing to surface reaction with residual organics. The decreased QY% is restored to the QY% of as-synthesized QDs by washing. However, the QY% of washed QDs also decreases with time, owing to the absence of surface passivation layer. On the other hand, the PMMA-treated QDs maintained a relatively higher QY% after washing than that of the washed QDs that were kept in toluene solution for 30 days. Formation of the PMMA coating layer on CdSe/ZnS QD surface is confirmed by HR-TEM and FT-IR. It is found that the PMMA surface coating, when combined with washing, is useful to be applied in the storage of QDs, owing to its long-term stability.

The objectives of this study, which filled gaps in previous studies, were: (1) to find the optimal mixing condition of nanoemulsions containing oleoresin capsicum (OC), Tween 80, propylene glycol (PG), and sucrose monostearate (SES) by microfluidization; (2) to investigate their properties and stability depending on such factors as pH, temperature, and heating time; (3) to measure the effect of adding ascorbic acid. In order to test these objectives, the following three experiments were conducted: Firstly, in order to find the optimal mixing ratio, nanoemulsions containing OC - the mean diameter of which is smaller than 100 nm - were prepared through the process of microfluidization; and their mean particle size, zeta potential, and capsaicinoids were measured. The test results indicated that the mixing ratio at OC : Tween 80 : PG + water(1:2) = 1 : 0.2 : 5 was optimal. Secondly, the properties and stability of nanoemulsions were investigated with varying parameters. The test results illustrated that single-layer nanoemulsions and double-layer nanoemulsions coated with alginate were stable, irrespective of all the parameters other than/except for pH 3. Thirdly, the properties of nanoemulsions were then analyzed according to the addition of ascorbic acid. The results demonstrated that the properties of single-layer nanoemulsions were not affected by addition of ascorbic acid. In case of alginate double-layer nanoemulsions, the particle size was reduced, and zeta potential increased with the addition of ascorbic acid. In conclusion, the demonstrated stability of various nanoemulsions under the different conditions in the present study suggests that these findings may constitute a basis in manufacturing various food-grade products which use nanoemulsions-and indicate that food nanoemulsions, if adopted in the food industry, have the potential to satisfy both the functionality and acceptability requirements necessary to produce commercially marketable food-grade products.

Edible insects have gained recognition worldwide as complementary protein sources. Recently, four edible insects were newly allowed to be used as food materials in Korea: the mealworm (Tenebrio molitor), the cricket (Velarifictorus asperses), the white-spotted flower chaffer beetle larva (Protaetia brevitarsis seulensis), and the rhinoceros beetle larva (Allomyrina dichotoma). In this study, we evaluated the oxidative stabilities of these four edible insects during cold storage. The insects were sacrificed by blanching for 3 minutes in boiling water. The blanched insects were then stored at 4℃ in an incubator for 42 days. The color values, titratable acidity, peroxide values, acid values, TBARS, contents of VBN, and total plate counts of the insects were measured at days 0, 2, 4, 7, 10, 14, 21, 28, 35, and 42, respectively. Blanching decreases oxidative stresses during storage. At day 0, the white-spotted flower chaffer beetle larva showed the highest values for acid value, TBARS, VBN, and microbial counts. Most of the oxidative indicators were significantly changed at day 14 in all four insects, possibly related with the growth on all microbial plates. Based on microbial safety and the oxidative stabilities of lipids and proteins, optimal storage conditions for the cricket, the white-spotted flower chaffer beetle larva, and the rhinoceros beetle larva were 10~14 days at 4℃. Likewise, the mealworm showed rapid oxidation after day 14, but poor qualities were not observed until day 28.

Graphite was diffusion-bonded by hot-pressing to W-25Re alloy using a Ti interlayer. For the joining, a uniaxial pressure of 25 MPa was applied at 1600 oC for 2 hrs in an argon atmosphere with a heating rate of 10 oC min−1. The interfacial microstructure and elemental distribution of the W-25Re/Ti/Graphite joints were analyzed by scanning electron microscopy (SEM). Hot-pressed joints appeared to form a stable interlayer without any micro-cracking, pores, or defects. To investigate the high-temperature stability of the W-25Re/Ti/Graphite joint, an oxy-acetylene torch test was conducted for 30 seconds with oxygen and acetylene at a 1.3:1 ratio. Cross-sectional analysis of the joint was performed to compare the thickness of the oxide layer and its chemical composition. The thickness of W-25Re changed from 250 to 20 μm. In the elemental analysis, a high fraction of rhenium was detected at the surface oxidation layer of W-25Re, while the W-25Re matrix was found to maintain the initial weight ratio. Tungsten was first reacted with oxygen at a torch temperature over 2500 oC to form a tungsten oxide layer on the surface of W-25Re. Then, the remaining rhenium was subsequently reacted with oxygen to form rhenium oxide. The interfacial microstructure of the Ti-containing interlayer was stable after the torch test at a temperature over 2500 oC.

본 연구에서는 돈지육 및 돈육 조직 내에 열안정성 수용성 단백질의 존재 여부를 확인하고 항체 생산에 있어항원으로의 사용 가능 여부를 확인하고자 하였다. 이를 위해 돈지육 및 돈육을 생(raw) 시료와 조리된(cooked) 시료로 구분하여 비열처리 및 열처리법으로 단백질을 추출한 후 단백질 존재여부를 단백질 정량과 SDS-PAGE로 확인하였다. 그 결과 돈지육과 돈육 모두 생 시료를 비열처리법으로 추출한 시료의 경우 25~100 kDa 사이의 다양한 단백질이 확인된 반면 시료를 가열하거나 추출 시 열처리를 한경우 돈지육에는 100 kDa 이상의 단백질과 30 kDa 및 15 kDa 이하의 일부 단백질이, 돈육에는 100 kDa 이상과 30 kDa 이하의 단백질이 확인되어 돈지육과 돈육에 열안정성 수용성 단백질이 존재하는 것으로 확인되었다. 이들 열안정성 수용성 단백질을 마우스에 면역 후 항혈청 역가를 측정한 결과 면역한 모든 마우스에서 높은 역가를 나타내었고, 생산된 혈청은 돈지육과 돈육에 각각 특이적인 반응성을 보인 반면 다른 축육과 지방육에 대해서는 반응성이 상대적으로 낮았다. 이러한 연구결과를 볼 때 돈지육 및 돈육에 존재하는 열안정성 수용성 단백질이 돈지육과 돈육에 특이적으로 반응하는 항체를 개발하는데 유용한 마커로서 활용이 가능하며, 열안정성 수용성 단백질에 대한 항체개발은 열처리된 축육 가공품 중 돈지육 및 돈육의 분석에도 매우 유용하게 활용할 수 있을 것으로 판단된다.

This study was conducted to provide important basic information about effective management of the marine environment at major inflow streams in Lake Andong and Lake Imha. The investigation was conducted 8 times from May, 2015 (AD1, AD2, IH1, IH2) to September, 2016 (AD3, AD4, IH3, IH4), and 8 surveyed sites were selected at Lake Andong (4 sites) and Lake Imha (4 sites). The inquiry identified 114 species, 59,913.7 inds. m-2 in Lake Andong and 112 species, 39,038.4 inds. m-2 in Lake Imha. The results indicate that the number of species and individuals in Lake Andong is more than that in Lake Imha, because Lake Andong has a variety of riparian vegetation and a richness of organic materials. Community analysis at Lake Imha revealed a dominant index of 0.57 (±0.18), a diversity index of 2.87 (±0.31), an evenness index of 0.73 (±0.04), and a richness index of 4.17 (±0.71). The results of functional feeding group analysis showed that a high proportion of species and individuals are gathering collectors. The results of functional habitat group analysis showed that a high proportion of species and individuals are clingers. The result of a physico-chemical water assay and dissolved oxygen and electric conductivity tests revealed that these measures increased when the water temperature decreased. The result of Pearson’s correlation analysis by biological factors and physico-chemical factors showed that species and electric conductivity are highly correlated with one another. Major inflow streams of Lake Andong and Lake Imha were exposed to various point pollution sources and non-point pollution sources. This implies a necessity for continuous monitoring of the aquatic ecosystems in order to effect systematic water quality management of Lake Andong and Lake Imha.

Stable Isotope Analysis (SIA) of carbon and nitrogen is useful tool for the understanding functional roles of target organisms in biological interactions in the food web. Recently, mixing model based on SIA is frequently used to determine which of the potential food sources predominantly assimilated by consumers, however, application of model is often limited and difficult for non-expert users of software. In the present study, we suggest easy manual of R software and package SIAR with example data regarding selective feeding of crustaceans dominated freshwater zooplankton community. We collected SIA data from the experimental mesocosms set up at the littoral area of eutrophic Chodae Reservoir, and analyzed the dominant crustacean species main food sources among small sized particulate organic matters (POM, <50 μm), large sized POM (>50 μm), and attached POM using mixing model. From the results obtained by SIAR model, Daphnia galeata and Ostracoda mainly consumed small sized POM while Simocephalus vetulus consumed both small and large sized POM simultaneously. Copepods collected from the reservoir showed no preferences on various food items, but in the mesocosm tanks, main food sources for the copepods was attached POM rather than planktonic preys including rotifers. The results have suggested that their roles as grazers in food web of eutrophicated reservoirs are different, and S. vetulus is more efficient grazer on wide range of food items such as large colony of phytoplankton and cyanobacteria during water bloom period.