본 연구는 거주시설에서 생활하는 지적장애 성인에게 놀이활동을 실시하여, 지역사회와의 통합과 사회화 에 필요한 자기표현과 의사소통에 어떠한 영향을 미치는지 밝히고자 질적 연구를 수행하였다. 장애인거주 시설에서 대학진학을 하지 않고 직업도 갖고 있지 않은 20세 이상의 지적장애인 7명을 대상으로 놀이활동 프로그램을 주 1회 120분씩 총 20회기를 실시하였으며, 이와 관련된 연구결과는 다음과 같다. 자기표현과 의사소통이 억제된 9명의 참여자에게서 눈 맞춤과 의사소통의 횟수가 증가되었고, 다양한 감정의 표현이 나타났으며, 언어적 표현이 부족한 참여자들은 몸동작으로 의사를 표현하였다. 참여자들이 서로에게 도움 을 주고받는 과정에서도 언어적, 비언어적 표현과 의사소통이 활발하게 이루어졌다. 놀이활동 프로그램의 경험을 통해 참여자들은 자신의 의사가 전달되는 과정을 거치면서 성취감을 가졌고, 다른 사람이 어눌한 자신의 언어를 알아듣지 못하는 것에 대한 두려움도 지속적인 도전을 통해 자신감을 회복하였다. 또한 자기표현과 의사소통이 반복되면서 사회화가 이루어지는 경험이 되었다.

The purpose of this study was to prepare Korean traditional vinegar using ginseng radices (wild simulated ginseng, red ginseng), and to investigate its functional ingredients and functionality. The total amino acid content of red ginseng vinegar and wild simulated ginseng vinegar was 124.36 mg% and 168.37 mg%, respectively, which was confirmed to be higher than the total amino acid content of red ginseng and wild simulated ginseng (70.71 mg%, 76.29 mg%). In addition, the polyphenol contents of red ginseng vinegar and wild simulated ginseng vinegar were 73.26 mg/g and 82.43 mg/g, respectively, which was confirmed to be higher than that of red ginseng and wild simulated ginseng (43.42 mg/g, 51.32 mg/g). And also, the DPPH electron donating ability of red ginseng vinegar and wild simulated ginseng vinegar was 893.26 μg/mL, 803.92 μg/mL, which showed that the scavenging ability was better than that of red ginseng and wild simulated ginseng (1253.21 μg/mL, 1021.01 μg/mL). On the other hand, it was confirmed that red ginseng vinegar and wild simulated ginseng vinegar contain 0.024% and 0.028% of compound K, a unique component of fermented ginseng, which is not contained in red ginseng and wild simulated ginseng. From these results, it was confirmed that Korean traditional vinegar using ginseng radices is a traditional functional food with functional ingredients and antioxidant activity.

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.

Lac 색소의 열수와 메탄올 추출물의 일부 유리아미노산과 그 유도체의 조성 및 함량, phenolic acid와 flavonoid의 조성 및 함량을 분석하고, 일부 피부미용 화장품 생리활성을 조사한 결과는 다음과 같다. 유리아미노산은 열수와 메탄올 추출물에서 각각 검출되지 않았으며, 단백질 비구성 아미노산 유도체는 열수 추출물에서 2종, 메탄올 추출물에서는 3종이 검출되었다. Phenolic acid와 flavonoids의 조성 및 함량분석에서, phenolic acid의 경우 열수 추출물에서 2종, 메탄올 추출물에서 5종이 검출되었다. 또한 flavonoids의 경우 열수 추출물에서는 검출되지 않았으나, 메탄올 추출물에서는 rutin과 taxifolin이 약간 존재하는 것으로 나타났다. DPPH radical 소거 활성은 메탄올 추출물이 40.43 ± 0.21%로 열수 추출물에서보다 약간 높게 나타났고, Tyrosinase 저해 활성은 메탄올 추출물이 열수 추출물에서 보다 약 2배 정도 높은 것으로 나타났다. HaCaT 세포주에 대한 세포독성은 모든 처리 농도에서 나타나지 않았으며, 처리 농도가 증가함에 따라 세포 생장이 증가하는 경향이었다.

One of the most demanded competencies of an oral health professional such as an oral pathologist is the ability to make clinical decisions. The purpose of this study was to examine the neurological differences between beginners and experts in the clinical decision-making process which consisting of complex, dynamic and sophisticated cognitive processes, and to identify the characteristics of experts based on the brain by comparing EEG activity. This study conducted experimental design with 10 dentists with more than 10 years of clinical experience and 10 students with short clinical experience as beginners as a fourth year student before graduation. The hypothesis-oriented algorithm proposed in HOAC II was used. As a standard, the clinical decision-making stage of the dentist was divided into three stages, and an event was developed and an experiment was designed for measuring EEG for each stage. The cerebral activity distribution patterns of the two groups were measured with EEG, compared and analyzed with pre-processing process using EEGlab. As a result, the difference in the auditory information was not noticeable between the novice and the expert, but the difference between the group of the novice and the expert appeared in the visual data and the radiographic data. In the case of beginners, the activity was higher in the left hemisphere, and in the case of experts, both brains were activated. The results of this study are intended to present objective information based on brain-based analysis of differences that appear in the clinical decision-making process of experts and beginners. It can be used as basic research data to present the brain-based characteristic analysis of dentist experts.

본 연구는 자원봉사활동의 허브 기능을 하는 자원봉사캠프 상담가의 참여 동기가 활 동유효성에 미치는 영향에 대하여 알아보고, 인정·보상에 대한 만족 수준이 이를 어떻게 매개하는지를 검증하여 궁극적으로 자원봉사캠프 상담가 활동 활성화를 위한 이론적·실 천적 함의를 제시하는데 연구의 목적이 있다. 본 연구결과는 다음과 같다. 자원봉사캠프 상담가의 참여 동기는 활동유효성에 직접적인 영향을 줄 뿐만 아니라 매개변인인 인정·보상을 통해 활동유효성에 간접적인 영향을 미치고 있음을 알 수 있다. 따라서 자원봉사캠프 상담가의 참여 동기는 활동유효성에 영향을 미치는 예측요인이 되 며, 자원봉사활동에 대한 인정·보상의 기능은 자원봉사활동의 동기유발 전략도구로 자원 봉사 만족도 및 지속성의 예측요인임을 밝히는 바이다. 이로써 본 연구는 자원봉사현장에서 준전문가로 활동하면서 자원봉사를 이끄는 자원 봉사캠프 상담가의 관리방안과 자원봉사활동 활성화를 위한 기초자료로 제공될 수 있다 는 점에서 그 의의를 갖는다.

본 연구는 평균수명 100세 시대를 살아가는 노년기의 여가를 어떻게 보내야 건강한 삶을 영위 할 수 있는가를 살펴보는데 있어 산림여가활동에 참여하는 노인의 건강이 심 리적복지감에 미치는 영향에 대해서 살펴보고 이에 여가활용 만족도의 매개효과를 중심 으로 관계분석을 통해, 노인의 다양한 여가활동들 중에서 특히 산림복지활동의 여가부 문에 참여하는 노인들의 삶의 질과 심리적 만족감을 향상시키기 위해 주관부서에서 더 다양한 프로그램들을 제공하고 정책화 시키는데 그 목적이 있다. 연구대상은 서울 및 경기도, 인천광역시 등의 수도권에 거주하는 61세 이상의 노인세 대 중에서 최근 1년 안에 산림여가활동 중에서 등산경험자, 도시숲․공원․휴양림 등의 산림(숲) 이용경험자 등을 조사대상으로 하여 판단표본법으로 추출하여 직접 설문하였 다. 설문 428부를 분석하여 사용하였다. 수집된 설문자료에 관한 통계분석은 사회과학 분야에서 활용되고 있는 SPSS 20.0 통계 패키지를 활용하여 모형을 분석하고, 연구모 형의 적합성을 검증하고, 변수들 간의 관계에 대한 가설을 검증하였다.

The aims of the present study were to confirm that regulation of the PA and environment via TGF-β regulation of sperm by Percoll-separated in porcine uterine epithelial cells. And, it was performed to identify the cytokines (TGF-β1, 2 and 3, TGF-β receptor1 and 2; interleukin, IL-6, IL-8) and PA-related genes (urokinase-PA, uPA; tissue- PA, tPA; PA inhibitor, PAI; uPA-receptor, uPAR) by spermatozoa. The experiment used porcine uterus epithelial cells (pUECs) and uterine tissue epithelial cells, Boar sperm were separated by discontinuous Percoll density gradient (45/90%), and tissues were co-incubated with spermatozoa, followed by real-time PCR. PA activity was measured of sperm by discontinuous Percoll density gradient (45/90%) for 24 hours. To measure viability and acrosome damage of sperm double stained propidium iodide (PI) and SYBR- 14 or FITC-PNA were used. In results, binding ratio of Percoll-separated sperm was found no differences, but sperms isolated from 90% Percoll layer reduced PA activity (p < 0.05). when co-cultured sperm selected Percoll in porcine uterus tissues epithelial cells, 90% layer sperm increased TGF-β R1, contrastively tPA and PAI-1 in comparison with control (p < 0.05). 45% sperm was decreased the expression of uPA (p < 0.05). TGF-β decreased PA activity in the supernatant collected from pUECs (p < 0.05). Especially, The group including uPA, PAI-1 were induce sperm intact, while it was reduced in sperm damage when compared to control (p < 0.05). Also, there was no significant difference group of tPA and tPA+I in the dead sperm and acrosome damage compared to control. The expression of tPA and PAI showed a common response. Percoll-separated spermatozoa in 90% layer reduced tPA and IL-related gene mRNA expression. Thus, Percoll-sparated sperm in 90% layer show that it can suppress inflammation through increased expression of TGF-β and downregulation of PA and IL in epithelial cells compared to 45% layer Percoll.

본 연구에서는 버섯의 건조 및 로스팅 처리의 가공방법에 따른 생리활성 성분과 영양성분의 변화에 대하여 알아보기 위해서 실험을 수행하였다. 항산화 등의 생리활성 성분의 변화를 분석한 결과, DPPH 라디컬 소거능은 잎새 버섯의 열풍건조 시료에서 가장 높았으며, 팽이와 꽃송이 버섯은 가공방법별 유의적인 차이는 없었다. 아질산염 소거능은 꽃송이버섯의 로스팅 시료에서 증가하는 것으로 나타났으며, 팽이와 잎새버섯은 유의적 차이가 없거나 생 시료에 비하여 낮았다. 총 폴리페놀 함량은 팽이와 꽃송이버섯 로스팅 시료가 생버섯(fresh) 시료에 비하여 증가 하는 것으로 나타났다. 아미노산 분석결과, 잎새버섯은 로스팅 처리로 인하여 아미노산 성분들 중 단맛을 담당하는 세린(Ser), 알라닌(Ala)성분들이 증가함과 동시에 쓴맛을 나타내는 류신(Leu), 이소류신(Ile) 성분들은 감소하는 것으로 나타났으며, 팽이와 꽃송이버섯은 필수 아미노산 성분을 포함하여 대부분의 아미노산 성분 함량이 로스팅 처리로 인하여 생시료에 비하여 증가하고 있는 것으로 나타나 로스팅 처리가 버섯내의 영양성분 향상에 효과가 있는 것으로 확인되었다.

본 연구는 중간관리자를 대상으로 리더십 변화신념, 리더십 개발활동, 상태불안과 이직의도 간의 경로를 분석하여 인과관계를 확인하고자 시도되었다. 이를 위해 중간관리자 600명을 표집하여 설문조사를 실시하였으며 수집된 데이터를 바탕으로 경로모형을 검증하였다. 본 연구 모형에 대한 직접효과를 살펴본 결과, 리더십 고정신념과 증진신념은 모두 리더십 개발활동에 정적 영향을 주었으며, 리더십 개발활동은 다시 상태불안에 부적인 영향을 미치고, 상태불안은 이직의도에 정적인 영향을 미치는 것으로 나타났다. 또한 리더십 고정신념이 상태 불안에 미치는 직접효과는 없는 것으로 나타난 반면 리더십 증진신념은 상태불안에 부적인 영향을 주었으며, 리더십 고정신념은 이직의도에 정적인 영향을 미친 반면, 리더십 증진신념은 이직의도에 아무런 영향을 미치지 않았다. 결과적으로, 리더십 변화신념인 고정신념과 증진신념이 각각 이직의도에 미치는 영향은 리더십 개발활동과 상태불안이 매개하고 있는 것으로 나타났으며, 여기서 리더십 개발활동은 상태불안과 이직의도를 감소시키는 중요한 보호요인으로 작용 하였다. 이러한 연구결과를 토대로 본 연구의 시사점과 제한점에 관하여 논의하였다.

Background: Patients with low back pain (LBP) experience misalignments in the center of pressure (COP) and muscle imbalances due to frequent onesided posture adjustments to avoid pain. Objectives: To identify the effects of Squat Exercises with Vertical Whole-Body Vibration on the Center of Pressure and Trunk Muscle Activity. Design: Randomized controlled trial. Methods: Thirty LBP patients with an imbalance in the COP were sampled and randomly assigned to an experimental group of 15 patients who under went an intervention involving squat exercises with vertical WBV and a control group of 15 patients who were treated via a walking intervention. As pretests before the interventions, the subjects’ COP was identified by measuring their stability index (ST), and erector spinae, rectus abdominis, transverse abdominis, gluteus medius muscle activity was analyzed by determining the % reference voluntary contraction (%RVC) value using surface electromyography while sit to stand. After four weeks, a post test was conducted to remeasure the same variables using the same methods. Results: Statistically significant differences were found in the ST (P<.01) and trunk muscle (P<.05, P<.001) in the experimental group before and after the intervention. In terms of the differences between the left- and right-side (RL) muscle activity, only the transverse abdominis (TrA) and gluteus medius (GM) exhibited statistically significant increase (P<.05). A comparison of the groups showed statistically significant differences in the TrA with respect to muscle activity (P<.05) and in the RLTrA and RLGM in terms ofthe difference between left- and right-side muscle activity (P<.01). Conclusion: Squat exercises with vertical WBV produced effective changes in the COP of patients with LBP by reducing muscle imbalances through the delivery of a uniform force. In particular, strengthening the TrA and reducing an imbalance in the GM were determined to be important factors in improving the COP.

Biosilica is a material extracted from the shell (cell wall) of Melosira nummuloides, a type of sea diatom, and is one of the widely distributed biominerals. Recently, some studies have revealed that biosilica has a characteristic bio-modulatory activity compared to synthetic silica. However, there has been little research on the effects and action mechanisms of biosilica in immune cells. In this study, we investigated the effect of biosilica water on mouse dendritic cells (DCs), the most potent antigen-presenting cells in immunity, and whether it may alter the function of DCs. Biosilica water decreased the metabolic activity of DCs at 20% concentration (v/v) and the production of IL-1 beta in a concentration-dependent manner. And also, more cells with fragmented nuclei were observed in the DCs treated with 20% biosilica water compared to other treatment groups. The mixed leukocyte response experiment showed the biosilica water-treated DCs significantly modulated the metabolic activity and proliferation of allogeneic spleen cells compared to control DCs. This result suggests that biosilica water may modulate the antigen-presenting capability of DCs. Taken together, this study shows the immunomodulatory activity of biosilica on DCs and may affect immune responses. Further research is needed to investigate the immunological activity of biosilica water.

본 연구는 푸드표현활동 프로그램이 노인의 주관적 기억력, 우울, 자기표현에 미치는 효과를 파악하기 위한 단일집단 사전사후 원시실험연구이다. 연구방법은 S시에 위치한 2곳의 경로당 이용 노인 대상자 36명이 참여하였으며, 2020년 1월 8일부터 2월 17일까지 총 6회기 프로그램을 시행하였다. 프로그램 전후에 주관적 기억감퇴, 우울, 자기표현의 변화를 설문지로 조사하였다. 중재 프로그램은 신체적 이완활동, 인지활동 및 다양한 ‘푸드’를 이용하여 손의 움직임과 자기표현을 할 수 있게 구성하였다. 연구결과, 푸드표현활동 프로그램은 대상자의 주관적 기억력(t=2.67, p=.011), 우울(t=3.88, p<.001), 자기표현(t=2.09, p=.044)을 통계적으로 유의한 차이가 나타났다. 본 연구 결과를 바탕으로 푸드표현활동 프로그램은 경로당 이용 노인의 우울 감소와 기억력 및 자기표현을 향상을 위한 간호중재로 활용될 수 있을 것으로 기대된다.

Buckwheat (Fagopyrum species) has long been an excellent functional food. Besides, buckwheat sprouts contain various functional substances. In this study, we investigated the antioxidant activity of buckwheat sprouts in the context of cultivars harvested after different cultivation periods (0, 3, 5, 7, 9, 13, and 15 days after planting). Buckwheat sprouts were cultivated at 25oC for up to 15 days and then extracted with ethanol. Antioxidant components were then extracted from sprouts and leaves using a freeze dryer. The total polyphenolic content, flavonoid content, and antioxidant activity were then analyzed. The total polyphenol content increased from 32.26 mg GA eq/100 g for raw buckwheat to 114.75 mg GA eq/100 g after 7 days of cultivation. Also, the flavonoid content increased from 20.61 mg catechin eq/100 g (0 days) to 56.54 mg/g after 9 days of cultivation. The DPPH radical scavenging activity (concentration of extract at 0.25 mg/mL) increased from 7.89% at day 0 to 53.48% after 9 days of cultivation. Additionally, the ABTS radical scavenging activity increased from 10.26% at day 0 to 32.89% after 7 days of cultivation; of note, the activity decreased afterward. These results suggest that the best buckwheat sprouts with higher biological activities are those cultivated for 7-9 days. For a complete understanding of the potential of buckwheat sprouts as functional foods, we plan to further analyze their antioxidant activity in the future.

Background: Because a forward-leaning posture can cause increased back muscle activity and pain. Therefore, an innovative method to reduce back muscle activity and pain is required. Objects: This study aimed to investigate the effects of a head support on muscle activity and pain in a forward-leaning posture. Methods: A total of 14 male and 16 female students (average age, 21.65 ± 2.37 years; height, 166.15 ± 7.90 cm; and weight, 60.65 ± 9.00 kg) were recruited for the experiment. Two of them were excluded due to musculoskeletal disorders. The muscle activity and pain in the forward-leaning posture were assessed while participants washed dishes for 7 minutes with and without a head support. The condition of using a head support was randomly performed with a 5-minutes break. To confirm a lumbar flexion angle of 30° during the experiment, myoVIDEO was used, and surface electromyography was used to measure muscle activity. Pain was assessed using a 10-point visual analog scale (VAS). The Wilcoxon signed-rank test was used to analyze the data, with p < 0.05 indicating statistical significance. Results: The cervical, thoracic, and lumbar erector spinae muscle activities significantly decreased with the use of the head support, but there was no significant change in the gluteus maximus. There was a significant decrease in the VAS score for the lumbar erector spinae (p < 0.05), but there was no significant change in the VAS score for the cervical region. Conclusion: The use of a head support in a forward-leaning posture reduced cervical, thoracic, and lumbar erector muscle activity and pain. Therefore, it could be recommended during working in a forward-leaning posture, such as during dishwashing, cooking, and working as a factory employee.

Background: Unstable surface-based core training can significantly enhance core strength, but no studies have compared the effects of balance pad- and sling-assisted exercises. Objects: To study the effects of unstable surface-based balance pad- and sling-assisted core strength exercises on muscle activity. Methods: Twenty male students aged 20–25 years participated in this study. The effects of three types of core strength exercises, performed with a sling or balance pad, on the activities of three muscles, i.e., the right musculus obliquus externus abdominis (EO), right erector spinae (ES), and right gluteus maximus (GM), were examined. Results: 1) In the glute bridge exercise, the percentage of maximum voluntary contraction of the EO, ES, and GM were significantly different between the balance pad- and sling-assisted exercises. The relative contribution of the ES and GM activities to all muscle activity were not significantly different between the two training types, whereas that for EO showed a significant difference. 2) There was no significant difference in the percentage value of maximum voluntary contraction (%MVC) among the EO, ES, and GM during the “leg-lifting with flat support”exercise, and there were no significant difference in the relative contributions between the two training types. 3) In the “side bridge leg separation exercise”, the %MVC of the ES, EO, and GM were significantly different between the two training types. Conclusion: Sling training for core muscles was generally better than balance pad assist training. The majority of physiotherapy patients require core training. Our results could guide physiotherapists in the choice of targeted exercises for these patients.

In this study, the antioxidative activity of Jeolpyun containing S. glauca (Bunge) Bunge powder (2%, 4%, 6%, and 8%) and extract (2%, 4%, 6%, and 8%) was evaluated based on the total polyphenol contents, electron-donating ability, scavenging of superoxide anion radical, and decomposition of hydrogen peroxide. Total polyphenol contents of Jeolpyun containing 8% S. glauca (Bunge) Bunge powder were the highest with a value of 2,280±0.00 μg GAE/mL. It was observed that total polyphenol content exhibited a significant relation with concentrations at p<0.05. The electron-donating ability of Jeolpyun containing 8% S. glauca (Bunge) Bunge extract was the highest with a value of 83.93±0.69%. The electron-donating abilities revealed significant differences between concentrations (p<0.05). The superoxide radical scavenging ability of the blank was 0.01523±0.00068 and Jeolpyun containing 6% S. glauca (Bunge) Bunge powder was the highest with a value of 0.00977±0.00842. The hydrogen peroxide decomposing ability of Jeolpyun containing 0% S. glauca (Bunge) Bunge powder was －0.00377±0.0049; 8% S. glauca (Bunge) Bunge powder revealed the highest decomposing ability of －0.0476±0.0234. Hydrogen peroxide decomposing abilities revealed significant differences between concentrations (p<0.05). Jeolpyun containing S. glauca (Bunge) Bunge powder and extract demonstrated improved antioxidative activities.