The base quality score recalibration (BQSR) is an important step in the variant calling from high-throughput sequence data. Motivated by the fact that BQSR necessarily requires a database of known variants such as the dbSNP, we present an extensive analysis on BQSR results for human and rice genome. We showed that the recalibration results depended on the size of the database. The more variants are there in the database, the larger averaged value of the recalibrated quality scores is obtained. This implies that the recalibrated quality score is lower than it should be when the number of variants in the database is not large enough. Based on the finding that the size of the database should play a crucial role in BQSR, we proposed a method to create a database when the size of a database is not large enough for BQSR results to be reliable. We demonstrated that, in the case of human, the database constructed by the proposed method generated almost the same results as the human dbSNP. In the case of rice, however, we showed that the proposed database is more reasonable than the rice dbSNP by illustrating how the proposed method is effective.

본 연구는 금강수계의 백제보 부근에서 이･화학적 수질의 시간적 변이 파악을 위해 2004 ~ 2017년까지 측정된 환경부 수질자료를 분석하였다. 이용된 수질 변수는 전기전도도(Electric conductivity, EC), 용존산소량(Dissolved oxygen, DO), 수온, 생물학적 산소요구량(Biological oxygen demand, BOD), 화학적 산소요구량(chemical oxygen demand, COD), 총인(Total phosphorus, TP), 총질소(Total nitrogen, TN) 및 총부유물(Suspended solid, SS)의 항목으로 이들의 수질특성은 연별, 계절별로 큰 변이를 보였다. 총인 및 이온희석현상의 지표로 이용된 전기전도도는 계절별 분석에서 장마기에 크게 감소하는 현상을 보이며 강우와 역상관관계(p<0.01, n=163)를 보였으나, 계절별 총인은 갈수기에 비해 장마기에 증가하는 특성을 보여 강우와 상관관계(p<0.01, n=163)를 보였다. 한편, 생물학적 산소요구량 및 화학적 산소요구량은 강우와 유의한 관계(p>0.05, n=163)를 보이지 않았고, 계절별 총부유물 농도는 강우기에 크게 증가했고 강우와 유의한 관계(p<0.01, r=0.6155)를 보였다. 생물학적 산소요구량의 계절적 변화패턴은 화학적 산소요구량과 유사한 양상(r=0.4751, p<0.01)을 보였으며, 마찬가지로 총질소의 변화 패턴 또한 총인과 유사한 양상(r=0.0579, p<0.01)을 보였다. 보 건설로 인한 수체의 정체와 유량 증가로 인해 총인과 총질소가 심층으로 침전되어 백제보 건설 전에 비해 건설 후에 감소하는 특성을 보였고, 용존산소량은 보 건설 후에 증가하는 현상을 보였다. 4대강 사업 후 물리적 환경변화에 기인한 수질 변화 분석 결과에서 녹조량 감소를 위해서는 지속적인 총인 관리와 총인 외에도 영양염류 조절, 생물학적 산소요구량 증가를 유발하는 비점오염원관리 등이 시급하다는 것을 알 수 있다.

Lettuce (Lactuca sativa L.) is one of the most important leafy vegetables in the world due to its health related benefits. Bitter sesquiterpene lactones (BSLs) are crucial secondary metabolites of lettuce which are useful in many physiological activities, and also has been used to relieve pain. This study was conducted to appraise the variability of phenotypic characteristics and the total content of BSLs in fifty lettuce germplasm. The germplasm collections exhibited wide variation in both qualitative and quantitative characters. The average plant weight was 333.3 g with a range from 60.0 to 700.0 g and the lactucin content was varied from 3.4 (IT265031) to 448.6 (IT300287) μg/g DW with an average concentration of 94.0 μg/g DW. Whereas lactucopicrin ranged from 6.8 (IT217859) to 2,714.9 (Superseonpung) μg/g DW with an average concentration of 805.8 μg/g DW. Total BSLs content was ranged from 19.6 (IT217859) to 2,821.9 (Superseonpung) μg/g DW with the average concentration of 899.8 μg/g DW. Significant (p≤0.05) differences in lactucin, lactucopicrin and total BSLs content were found among the germplasm collections. Leafy type lettuce germplasm collections originated from South Korea revealed the highest average total BSLs content (2,821.9 μg/g DW). Principal component analyses of lactucin and lactucopicrin generated based on leaf color and plant growth type yielded two principal components, PC1 and PC2, which accounted for 60.8 and 39.2 % of the total variance, respectively. Our study provides fundamental insights on phenotypic characteristics and the total content of lactucin and lactucopicrin of lettuce germplasm which may further help researchers and breeders to produce improved lettuce varieties.

In this paper, the result of application of this simple method of vibration analysis developed by the author, to the special orthotropic plates with variable cross-section, and with a pair of opposite edges simple supported and the other pair of opposite edges free is presented. This problem represents the simple-supported single span bridge system without effective longitudinal edge beams. The effect of concentrated point mass/masses is also studied.

본 연구는 콩 콤바인 수확 시 수확장애와 종실의 품위를 떨어뜨리는 잎과 줄기의 노화 지연에 관한 연구로 2015~2016년도 국립식량과학원 남부작물부(경남 밀양시 소재)의 시험포장에서 수행하였다. 시험품종은 대원콩과 풍산나물콩을 사용하였으며, 6월 9일에 고휴 2열로 휴폭 110 cm, 주간거리 40 cm, 1주 2본 재배하였고, 콩 꼬투리를 0~50%범위에 수준별로 제거하여 성숙기 생육특성과 잎과 줄기의 건물중에 대해서 조사하였다. 주요결과를 요약하면 다음과 같다. 1. 대원콩과 풍산나물콩 모두 종실비대시 콩 꼬투리 제거가 생육특성에 영향을 미치지는 않았지만, 제거 비율이 높아질수록 성숙기가 지연되었다. 2. 콩 꼬투리 제거 비율이 높아짐에 따라 종실 무게도 증가하였는데 꼬투리의 제거비율이 대원콩은 20%, 풍산나물콩은 30% 이상일 경우에는 차이가 없었다. 3. 꼬투리 제거비율이 높아질수록 잎과 줄기의 건물중이 증가하였는데, 이는 종자에 축적되어야 할 잉여 동화 산물이 잎과 줄기에 축적된 결과로 보여진다. 4. Sink/source 감소폭은 대원콩 20% 제거 시 0.18, 풍산나물콩 30% 제거 시 0.42로 이전 처리구에 비해 크게 감소하였기 때문에 잎과 줄기의 성숙이 불리할 것으로 판단된다.

1. 주요 쌀 생산국 브랜드 쌀 및 MMA 쌀 40점의 품질을 조사한 결과, 수분함량은 11.6(인도)~14.2%(중국), 단백질 함량은 5.82(미국)~7.86%(중국), 아밀로스 함량은 16.2(태국)~24.4%(인도), 윤기치는 45.2(인도)~75.7(미국)의 범위를 나타내었다. 2. 국가별 수집된 브랜드 쌀의 형태적 특성을 살펴본 결과, 중국과 베트남의 브랜드 쌀은 주로 통통한 단립종이었고, 미국 브랜드 쌀은 중간 두께의 중립종이었으며, 태국 브랜드 쌀은 얇은 장립종의 형태를 나타내었다. 호주와 인도는 단립, 중립, 장립의 다양한 형태의 쌀을 확인 할 수 있었다. 3. 국가별 브랜드 쌀의 백미 품위를 살펴본 결과 완전미율은 72.4(인도)~95.2(태국)%의 범위였으며, 베트남 브랜드 쌀은 동할미 비율이 높았으며, 인도 브랜드 쌀은 분상질미 비율이 높은 특징을 나타내었다. 4. RVA를 이용한 호화특성을 분석 한 결과, 인도의 브랜드 쌀이 최고온도가 낮고 치반점도가 높으며 강하점도가 낮아 취반미의 찰기가 낮을 것으로 예측되었으며, 호화개시온도 또한 유의하게 높은 것을 확인할 수 있었다. 5. 국가별 브랜드 쌀의 식미 관능평가를 실시한 결과, 수집된 브랜드 쌀의 평균 종합기호도는 미국(-0.59), 중국(-0.76), 호주(-1.23), 베트남(-1.43), 태국(-2.09), 인도(-2.49) 순으로 나타났다.

Kang, Hyeon-Seok. & Kim, Minji. 2018. “A Multivariate Analytical Study of Variation Patterns of Honorific Final Endings in KakaoTalk Dialogue”. The Sociolinguistic Journal of Korea 26(1). 1~30. On the basis of KakaoTalk conversation data, this paper investigates the variations found in the use of honorific final endings, expecially haeyo and hapsyo styles. For this study we collected 3,087 tokens of honorific final endings from the dialogue of 8 KakaoTalk group chat rooms. Through multivariate analyses using Goldvarb and LVS, we examined whether the linguistic and external constraints, which were claimed by Kang (2011) to constrain the usage of haeyo and hapsyo styles in spoken language, also operate in the KakaoTalk dialogues. The results of the analyses revealed not only far more variants in the KakaoTalk data (12 variants of haeyo and 6 variants of hapsyo) than in spoken language but also various additional variants realized with pronunciation spelling and partial omission of final endings. As in the spoken language, ‘sentence type’, ‘speaker's gender’, ‘rituality of utterance’, and ‘formality of situation’ were analyzed as constraints which had a strong effect on the variation. In the data examined, besides haeyo and hapsyo styles, new honorific styles of hao, hasoseo, seupjyo, handayo, hasem/hasam were also found. Two new types of variants which was not yet discussed in previous studies, were also observed in the data: variants of ‘syam’ and ‘syeom’ of hasem/hasam styles and two variants of ‘yo’ realized with consonant addition to the onset position.

This experiment was performed to analyze the annual variation of growth traits of soybean to determine the information of less sensitive traits and stable cultivars based on the environmental conditions. Sowing was carried out on June 2 for each year and the test plot was arranged in three replicates by randomized complete block design with thirty soybean varieties including two landraces during the period 2014-2015. The weather conditions during the test period were quite different with extremely low precipitation and longer sunshine duration in 2015. The variation of characteristics related to growth period such as days of growth, days of maturity, days of flowering and 100-seed weight were less in spite of different environmental conditions. While the variation of the number of pods per plant was high. Considering growth and seed characteristics like the number of pods per plant, the number of seeds per pod, and 100-seed weight which are linked directly to the yield, cultivars such as Shingi, Daewonkong, Danbaegkong, and Daepung for beancurd and soypaste, Pungwon, Haepum and Shingang for soy-sprout and Seoritae for cooking with rice were more stable and are expected to have high yield in Suwon, the south-central part of South Korea. These results are useful for the selection of breeding resources to develop cultivars with high stability under changeable weather condition.

Kang, Hyeon-Seok & Kim, Minji. 2017. “Aspects of Variations of ‘ye’ and ‘ne’ Observed in the Dialogues of the Instant Messenger KakaoTalk”. The Sociolinguistic Journal of Korea 25(3). 1~27. This paper investigates the variations found in the use of ‘ye’ and ‘ne’ in the conversations of the mobile messenger KakaoTalk. This study was conducted based on the dialogues from the 159 KakaoTalk chat rooms, using the statistical package Language Variation Suite. As many as 17 variants of ‘ye’ and ‘ne’ were observed in the data analyzed compared to just the four variants of the speech data (Kang 2009), revealing a big difference between the two data types. Further, the ‘ne’ variants were absolutely dominant, comprising 90% of the tokens, making them gender-neutral in this variation. Regression analyses showed that ‘gender’ was the most important constraint influencing the variation: Men used the ‘ye’ variants 25% of the time, while women used them only 4% of the time. Age was another important factor: Older people used the ‘ye’ variants more than younger people. This study reveals that the new medium of net language (Crystal 2001) could produce radically different results from spoken language even when the same sociolinguistic variables are analyzed, and it may point to a new direction of research in variation theory.

The objectives of this study were to select the seeds of Cucurbitaceae and Solanaceae genotypes in terms of superior with bioactive compounds content and to inform sophisticated data for developing the high value-added products. We evaluated to aspects of the antioxidant activity, polyphenol content, and flavonoid contents in seeds from two vegetable family. We used in the Cucurbitaceae(watermelon, squash, bitter gourd, and sponge gourd) and Solanaceae(hot pepper, sweet pepper, and egg plant) the total 408 genotypes. In Cucurbitaceae, polyphenol content of watermelon and squash genotypes were ranged 19.9-343.8 and 6.1-81.2 mg·100 g-1 DW, respectively. The polyphenol content of watermelon genotypes was 12% among all genotypes over 160 mg·100 g-1 DW. The mean of flavonoid content in watermelon and squash genotypes represented 80 and 41.3 mg·100 g-1 DW, respectively. In Solanaceae, flavonoid content of hot pepper genotypes was ranged 64.4-472.5 mg·100 g-1 DW, with an average of 165.0 mg·100 g-1. The 23 hot pepper genotypes were classified over 90% antioxidant activity. The antioxidant activity of sweet pepper was ranged 35.9-90.3%, and 23% of all genotypes represented 82% antioxidant activity. The polyphenol and flavonoid content of egg plant was ranged 38.1-642.0 mg·100 g-1 DW and 14.2-1217.0 mg·100 g-1 DW, respectively. In addition, we selected that 8 egg plant with the superior genotypes for antioxidant activity, polyphenol, and flavonoid content. Results revealed that there was significant variation of antioxidant activity and bioactive compounds contents in both vegetable famaily. In addition, we suggested that selected genotypes seeds with high contain bioactive compounds will be more efficiency to develop natural value-added products.

This study was carried out to obtain useful information on the cost-effective and labor-saving methods in the on-farm pigmented rice cultivation trials that were performed in Gangjn, Jindo and Gimje provinces. Two different pigmented rice cultivars were tested at each site from 2014 to 2015. From the common rice transplant technologies, Wet Hill Direct Seeding Technology (WHDST) was applied and was compared to Transplanting Machine (TM) Technology, which was mainly used for rice transplanting. The results of the study showed that during the trial period, the average C3G contents of pigmented rice ‘Heugkwang’ in Gangjin was 145.5 mg/100g using the WHDST. This was 23.5 mg/100g higher than using TM in Gangjin area. On the other hand, the C3G contents of functional pigmented rice variety ‘Seonhyangheukmi’ was 163 mg/100g under the WHDST while 189.3 mg/100g under the TM. Therefore, it was observed that the C3G contents of functional pigmented rice changed depending on the variety and according to the annual environmental variation. In Jindo area, two years average C3G contents of functional pigmented rice variety ‘Josaengheugchal’ was resulted to 268.1 mg/100g using TM. This C3G contents was 97.8 mg/100g more than the amount obtained using WHDST. For functional pigmented rice variety ‘Shinnongheugchal’, the C3G contents was 247.3 mg/100g in the WHDS, while 249.1 mg/100g in the TM. This indicated that C3G contents tended to be similar based on cultivation method. The C3G contents of the rice variety ‘Heugkwang’ in Gimje area were 34.7 mg/100 g and 20.2 mg/100g using WHDST and TM, respectively. Meanwhile, the C3G contents of functional pigmented rice variety ‘Heugsujeong’ was 85.6 mg/100g in the WHDST and 43.62 mg/100g in the TM. In conclusion, the study showed that the C3G contents of the pigmented rice are affected by the yearly environmental variation, the regions, and the cultivated variety.

Variations among 15 major waxy cultivars in terms of milling appearance and cooking characteristics at different moisture contents were investigated. Hardness of milled rice kernels increased and 1000-grain weights decreased with the reduction of moisture content. The milled rice kernels showed about two-fold hardness gap between 12 and 16% moisture contents. The 1000-grain weights revealed 6% reduction from 21.4 g to 20.2 g at 16% and 12% moisture contents, respectively. The whiteness of waxy rice which indicated varietal variation also increased with decrease of moisture contents. In most cultivars, the whiteness of waxy rice peaked and stabilized at around 14% moisture content. In milling properties, brown/rough rice ratios were not affected by moisture content, however, milled/brown rice ratios increased with the decrease of moisture contents between 14 - 16%. This implied that the proper moisture content for milling rate of waxy rice is lower than that of non-waxy rice. The chalkiness expression of milled waxy rice varied directly with moisture contents. At 16% moisture content, the rates of translucent and semi-translucent kernels like non-waxy rice were 49.0 - 84.4% while at 14% moisture content, the rates of chalky rice were 88.7 - 99.9%. In terms of cooking properties of milled waxy rice with different moisture contents, lower moisture contents (12 - 13%) were related with higher water absorption rates. The higher volume expansion of cooked milled rice and more soluble solid after cooking in most cultivars showed the possibility of low palatability of cooked waxy rice with lower moisture contents (below 13%).

무당벌레(Harmonia axyridis)는 종내에서 초시색상패턴이 매우 다양하게 존재한다. 본 논문에서는 서로 다른 색상패턴의 무당벌레를 대상 으로 amplified fragment length polymorphism (AFLP)을 실시하여 무당벌레의 초시색상 패턴간 유전형질의 차이를 확인하고자 하였다. 총 28 개의 프라이머 조합으로 실험을 실시한 결과, 총 2,741 개의 밴드가 검출되었다. 그 중 20 개의 밴드(S1-S20)만이 특정 색상패턴에서 나타났다. 이들 가운데 9 개의 밴드를 색상에 연관된 AFLP 후보 지표로 선발하였다. 밴드 가운데 S1과 S2, S20은 Succinea 1, 2 변이형에 공통적으로 나타났으며, S3와 S5는 Conspicua 변이형에 특이적이었다. 또한 S13는 Spectabilis 변이형에, S15와 S18, S19는 Succinea 2 변이형에 특이적이었다. 특정 색상패턴에만 나타나는 9 개의 AFLP 지표들은 cloning을 통해 염기서열 분석을 실시하였고, GenBank를 이용해 다른 염기 서열과 비교를 해보았지만 아무런 상동성도 찾을 수가 없었다. 무당벌레 종 내 유전적 다양성을 평가한 결과, Spectabilis가 Conspicua보다 Succinea 변이형에 높은 유사성을 보였다. 색상에 연관된 AFLP 후보 지표를 기준으로 sequence characterized amplified region (SCAR) 지표로 변환하여 9 개의 AFLP 분자지표들 가운데에서 5 개만이 SCAR 지표로 전환될 수 있었으며, 이를 통해 AFLP 지표가 무당벌레의 색상과 연관되어 있는지 확인할 수 있었다.

Chinese characters underwent the process of conversion of seal script to clerical script, transitioning from lines to strokes in composition. the degree of tokenization components increased markedly. Some characters could no longer be classified by radicals as explained under the “unified by form and meaning” principle described in Shuowen Jiezi (《說文解字》). Thus in the Ming Dynasty, Zihui (《字彙》) eliminated and combined many of the radicals in Shuowen Jiezi (《說文解字》), and adopted the principle of “classification under form and not meaning” for classification purposes. The Kangxi Dictionary (《康熙字典》) from the Qing Dynasty continued the tradition of 214 radicals included in Zihui (《字彙》). Though radicals were established and classified according to two principles: convenient perusal and being true to the meaning of characters created by ancients, the form and meaning of characters become disassociated after conversion of seal script to clerical script, though the original theory of character creation which linked external composition of character with internal meaning was passed on. As the two radical classification principles of classification through form and classification through meaning conflicted with one another, the book adopted the principle of radical classification through form to facilitate convenient perusal. Thus, some radicals were iconized, losing their function to convey meaning or sound in characters. From the perspective of the development of clerical script, clerical script from the Qin Dynasty (qinli) is also referred to as “ancient clerical script” (guli), an early form of clerical script during this transition period between ancient and modern characters. Development of clerical script reached maturation in the Han Dynasty, with the Eight Points (bafen) script. This paper attempted to organize the mark process that Chinese characters underwent during the conversion of seal script to clerical script, through utilizing ancient texts which have been unearthed, Han Dynasty steles, and texts from Qin and Han Dynasty bamboo and wooden slips unearthed in the past few decades as research material. The hope was to uncover the evolutionary process and original form of iconized radicals as they formed.

Sesquiterpene lactones (SLs) are a group of over 500 compounds, characteristic of the Asteraceae. They are interesting from the chemical and chemotaxonomic point of view, and show antitumour, anti-leukaemic, anti-cardiovascular disease, reduction of inflammation, and anti-microbial activities. The SLs, free lactucin and lactucopicrin, content in 572 accessions of lettuce (Lactuca sp.) germplasm introduced from 30 countries were quantified using high-performance liquid chromatography. Variation range of free lactucin content exhibited trace ~ 235.3 μg from 1g of dried leaves and average was 41.2 ± 1.2 μg (Avr ± SE), variation range 55.0 ~ 3,041.0 μg and average 526.9 ± 17.5 μg in free lactucopicrin content, and variation range 66.3 ~ 3,188.5 μg and average 568.1 ± 18.1 μg in total free SLs content. Lactucopicrin occupied 92.7% of the total SLs content. Among the varietal types, crisphead type exhibited the highest average total free SLs content, next is leaf lettuce, and butterhead type lettuce exhibited the lowest that of content. German accessions exhibited the lowest average total free SLs content, Korean accessions exhibited the highest, and European origin accessions exhibited lower that of content. Red leaf color accessions having higher SLs content than that of green color. Seven accessions having more than 2,000 μg·g-1 dwt of total free SLs content and five accessions having less than 100 μg·g- 1dwt that of content. These accessions can be used as low SLs content cultivar breeding or high SLs content cultivar breeding sources as well as research materials for medical treatment such as, anti-tumour, anti-leukaemic, and antimicrobial activities, etc.

Cho Yong‐joon･Ha Ji‐hee. 2016. “On the sociolinguistic variation of Korean mirative markers ‘‐kuna’, ‘‐ney’ and ‘‐ta’”. The Sociolinguistic Journal of Korea 24(1). 241~269. The purpose of this paper is to investigate the sociolingustic variation of Korean mirative markers ‐kwuna, -ney and ‐ta, through an experimental method. A 7‐point Likert scale task was adopted for this purpose. The participant’s age was the significant social factor of the observed linguistic variation, but gender does not play any significant role. Particularly, the elderly generation prefers ‐kwuna for a mirative marker, but this tendency declines for young generation. ‐ta seldom functions as a mirative marker for elderly generation, but it gets an independent status as the mirative marker for young generation. The negative semantico‐pragmatic properties of ‐kwuna affects this change.

서로 다른 숙기를 가진 여섯 개의 자두 품종을 이용하여 과실 발육 단계에 따른 주요 과실 특성의 변 화를 조사하였다. 자두 과실의 산도는 유과기에 가장 높은 수치를 보였다가 과실의 발육이 급속하게 이 루어지는 시기를 기점으로 감소되고 이후 수확기까지 점진적으로 감소하였다. 가용성 고형물 함량은 산 도와 거의 반대로 증가하는 경향을 보였고 숙기가 빠른 품종 일수록 보다 빠른 시기에 변화가 나타났 다. 수확기에 측정 된 산도와 가용성 당함량의 축적량은 품종에 따라 차이가 있어 품종 특이적인 변이 가 있음을 확인하였다. 과실의 발육동안 유리당과 유기산의 조성변화와 수확기에 조사된 총 유리당과 유기산 함량의 검정 결과에서도 품종에 따른 변이를 관찰하였다. 이를 통하여 과실의 발육과정 중 일어 나는 품종 특이적 과실특성의 다양한 변화 양상이 결과적으로 수확기 품종 간 과실품질에 있어서 변이 를 일으키는 주요한 요인으로 작용하고 있음을 간접적으로 확인하였다. 과실의 특성과 관련된 형질들의 값을 이용하여 주성분 분석을 실시한 결과, 본 실험에 이용된 여섯 개의 품종을 대조적인 과실 특성을 가진 세 개의 그룹으로 나눌 수가 있었다. 본 연구를 통해서 밝혀진 과실발육 기간 동안과 수확기에 대 조적인 과실특성을 가진 품종들을 이용한 추가적인 생리 및 육종 연구들은 고품질 자두의 육종에 도움 을 줄 수 있을 것이다.

One hundred seventy two accessions of safflower, collected in four countries were investigated for their morphological and biochemical characters in 2014. The accessions were categorized into two groups; South-Central (S-C) Asia and South-West (S-W) Asia, and each group was represented the accessions of two countries. Variation in morphological and biochemical characters was observed between two groups of accessions. The average value of seed weight and range of variability were higher in S-C Asia accessions while S-W accessions exhibited the variation in plant height, leaf length and days to flowering. The average value of oleic and total oil content were higher in S-C Asia accessions, and the values were 19.8%, and 231.4 mg.g-1, respectively, while the range of variability for total oil content was higher in S-W accessions. Plant height exhibited a significant positive correlation with days to flowering (r = 0.625**). Palmitic acid had positively correlated with stearic acid (r = 0.282**) and linoleic acid (r = 0.444**). Oleic and linoleic acid showed a strong negative correlation (r = -0.977**). The first three principal components explained 57% of the total variation. Morphological and biochemical variation exist in different groups of accessions could be useful to breeder for developing new safflower cultivars with high oil quantity and quality.

기후변화는 국내 해충상의 변화에 영향을 주는 주요 요인 가운데 하나로 알려지고 있다. 특히 지구 온난화 모델에 따라 남방계 곤충의 서식 지 확대가 예상되고 있다. 휴면 기작을 가지고 있지 않은 파밤나방(Spodoptera exigua)과 배추좀나방(Plutella xylostella)은 시설재배지를 중심으 로 국내에서 월동이 가능한 것으로 알려지고 있다. 그러나 두 해충은 계절적 발생 양상에서 뚜렷한 차이를 보여주었다. 배추좀나방은 이른 봄철과 가을기간에 발생하고 여름 기간 중에는 발생하지 않았다. 반면에 파밤나방은 늦은 봄철에 나타나기 시작해 가을까지 지속적으로 발생하였다. 본 연구는 이러한 두 남방계 곤충이 계절적 발생 차이를 보이는 것이 이들이 갖는 고온에 대한 감수성 차이에 기인한 것으로 가정하였다. 이 가설을 증명하기 두 곤충의 내열성을 비교 분석하였다. 동일한 열처리(42℃)에서 배추좀나방 유충은 40 분 노출에 100% 사망률을 보인 반면, 파밤나방 은 대부분의 유충이 80 분의 노출에서도 생존하였다. 이러한 내열성은 두 곤충 모두 이들의 발육시기에 따라 상이했다. 배추좀나방은 4령 유충과 성충이 가장 높은 내열성을 보인 반면, 파밤나방은 1령 유충에서 가장 높게 나타냈다. 두 곤충 모두는 37℃에서 30 분간 전 처리 후 고온에 노출시 키면 생존율이 뚜렷하게 증가했다. 이러한 내열성유기는 두 곤충 모두 혈림프의 글리세롤 함량 증가와 관련성을 보였다. 또한 파밤나방의 경우는 열충격단백질의 발현도 증가하였다. 따라서 이상의 결과는 여름기간 배추좀나방의 발생이 없는 것은 이 곤충의 고온에 대한 높은 감수성에 기인 된 것으로 보이고, 반면에 파밤나방은 비교적 높은 내열성을 보유하여 여름 기간에도 발생을 지속시킨 것으로 해석되었다.