About 70% of total honey products produced by Korean bee keepers was acacia honey. The remaining 30% was chestnut honey, jujube honey, snowbell honey, and another honey. False acacia, went down in payability since the middle 2000s because of simultaneous blooming etiolation chlorsis decreases productivity after aging. Therefore, substitution honey plants were necessary. This study estimated nectar secretion amount of each flower and productivity per ha at 14 medical herbs. Each flower, Codonopsis lanceloata, estimated a majority nectar secretion amount at 176.08 ul for each of the 14 medical herbs. Astragalus membranaceus estimated majority nectar secretion amount at 1273.3 L per ha for each of the 14 medical herbs. Medical herbs were hypothesized with valuable honey plants.

본 연구는 국내 유통 감초약재의 원산지 판별을 위해 수행되었다. 이를 위해 약재로 쓰이는 만주감초(Glycyrrhiza uralensis) 및 유럽감초(G. glabra)와 약재로 사용되지 않는 국내 자생종 개감초(G. pallidiflora) 식물의 형태적 차이 및 엽록체와 핵 DNA 구간의 염기서열의 차이를 구명하였다. 감초식물 3종의 형태적 특징을 비교한 결과, 잎과 뿌리에서 차이를 보였다. 특히 잎의 형태에 있어서 중국감초와 유럽감초는난형 혹은 타원형인 반면, 개감초는 엽장/엽폭비가 큰 피침형이었다. 또한 건조된 뿌리로 시중되는 감초 한약재의 경우, 중국산과 한국산은 백색인 반면 우즈베키스탄산은 황백색이었다.감초식물 3종을 대상으로 DNA 수준에서의 차이를 비교한 결과 rpoB2, rpoC1에서는 G. uralensis와 G. glabra가 구분되지않았으며, psbA-trnH의 경우 단 한 구간의 SNP에서 차이를 보였다. 반면 ITS2에 의해 증폭된 450bp의 염기서열을 비교한 결과, G. glabra는 98, 99, 100번째 위치에서, G. pallidiflora는 137, 161, 164, 203, 296위치에서 감초 종간판별을 위한 특이적 SNP가 확인되었다. 또한, phylogenetic tree 분석을 통해, 약재로 쓰이는 G. uralensis과 G. glabra는 약재로 쓰이지 않는 G. pallidiflora에 비하여 유전적 거리가 가까움을 확인하였다. 본 표준시료에서 얻은 결과를 유통 한약재 원산지 판별에 적용한 결과, 중국산과 한국산의 한약재는 G. uralensis와 염기서열이 일치하였고, 우즈베키스탄으로부터 수입된 한약재는 G. glabra와 염기서열이 일치됨을 확인하였다. 이에 감초의 생체 및 한약재의 원산지 판별을 위해 ITS2를 이용한 분자수준에서의 판별이 유용하다고 판단된다.

본 연구는 아토피 피부염을 갖는 질환동물에서 어성초를 비롯한 다양한 식물추출물을 이용하여 제조한 크림의 항아토피 피부염증에 대한 효능을 평가하였다. BALB/c 마우스 를 이용하여 아토피 피부염 유발 대조군 및 크림 처리군으로 완전 임의 배치하였다. 혈액 IgE와 히스타민 농도는 크림 처리군이 DNCB 유도 아토피 대조군에 비해서 유의하게 감소하였으며, 아토피 피부염이 심각한 상태의 마우스에서 정상적인 피부상태로 회복되었음을 관찰하였다. 본 결과는 아토피질환 모델 BALB/c 마우스에서 어성초 혼합 식물추출물의 투여가 혈액 IgE를 낮춤과 동시에 비만세포에서 생성되는 히스타민의 배출량을 억제함으로써 항아토피 효능을 갖는다는 점을 시사 해준다.

The increase in the consumption of herb medicines have made their use a public health problem due to the potential fungal contamination and the risk of the presence of mycotoxins. 360 samples of herb medicines were evaluated for the aflatoxin contamination. The natural occurrence of aflatoxins in these samples were determined using immunoaffinity column clean up and high performance liquid chromatography (HPLC) with post-column derivatization. For samples analyzed, mean levels (incidence) of AFB1, AFB2, AFG1 and AFG2 in positive samples were 1.4 μg/kg (46.4%), 0.4 μg/kg (25.4%), 1.1 μg/kg (37.8%) and 0.9 μg/kg (24.3%), respectively. Recoveries of the full analytical procedure were 71.7~99.7% for AFB1, 88.1~99.2% for AFB2, 82.8~95.5% for AFG1 and 77.9~90.0%for AFG2. The excess cancer risk estimated using the cancer potency of aflatoxin B1 (7 (mg/kg/day)−1 for HBsAg− and 230 (mg/kg/day)−1 for HBsAg+) were 1.30 × 10^(−5) ~ 1.22 × 10^(−7) for hepatits B surface antigen negative (HBsAg−) and 3.31 × 10^(−4)~ 3.12 × 10^(−6) for hepatits B surface antigen positive (HBsAg+) respectively. In conclusion, although the contamination levels of samples used in the study were low, further actions are also required to undertake a program of herbal surveys in order to access mycotoxin contamination overall so that the safety of public will be protected.

Our previous study demonstrated that fermented four-herb combination (Ulgum, Yagmomil, Maesil and Bokbunja) with probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis) (hence, this additives was designated PFH) have immune enhancing and antibacterial effects in broiler chickens. The aims of the present study were to investigate the effects of PFH on growth performance and meat quality of broiler chickens. Twenty-day-old Ross broiler chickens were fed with or without PFH for 2 weeks. Body weight gain of the 2% PFH-fed group was significantly increased compared with those of the control group (*P<0.05). Moreover, body weight gain of the 1% PFH-fed group was also showed a increased tendency compared with the control group, although the difference was not significant (P=0.06). However, no significant difference in feed intake was observed among the groups. Feed conversion ratio of the 2% PFH-fed group was significantly decreased compared with those of the control group (*P <0.05). In addition, feed conversion ratio of the 1% PFH-fed group was also showed a slightly decreased tendency compared with the control group, although the difference was not significant (P=0.07). However, no significant differences in the proximate composition (moisture, crude fat, crude protein and crude ash) and the physico-chemical index (cooking loss, shearing force, water holding capacity and pH) were found among the groups. In addition, succulence, tending and flavor in meat of all 3 groups were also not different. Taken together, these findings suggest the possibility that PFH could be used as the alternative of antibiotic growth promoters by improving the performance of broiler chickens.

본 연구는 축제만족도 및 이용행태를 분석함으로써 향후 허브축제의 계획 및 프로그램 구상을 위한 기초자료를 제공하기 위하여 2010년 허브축제 방문객을 대상으로 진행되었다.2010년 남원허브축제는 운봉읍 용산리 일원에서 개최되었으며 허브홍보, 판매, 전시관 및 체험프로그램이 운영되었으며인근의 경관농업지구와 연계하여 다양한 볼거리를 제공하였다.지인을 통한 홍보효과가 높게 나타났으며 연령별로 축제개최 인지방법이 달랐다. 방문객의 절반가량이 축제를 목적으로방문하였으며 바래봉 철쭉 감상을 목적으로 방문한 경우도 상당한 비율을 차지하였다.방문객은 축제 전반에 대하여 보통보다 약간 높은 만족도로 평가하였다. 행사내용, 서비스, 안내팜플렛, 볼거리,체험거리, 안내시설을 독립변수로 하는 다중회귀분석을 실시하여 방문객의 만족도에 영향을 미치는 효과적인

꿀풀과 허브의 부위별 메탄올 추출물에 대한 항산화, 항암, tyrosinase 저해활성을 검정한 결과는 다음과 같다. 총 폴리페놀과 플라보노이드 함량을 검정한 결과, lemon balm의 leaves 추출물에서 629.48±0.90 mg GAE/g DW로 가장 높은 폴리페놀 함량을 확인하였고, rosemary의 whole 추출물에서 22.45±0.81 mg CE/g DW 로 가장 높은 플라보노이드 함량을 나타내었다. Rosemary(whole) 추출물은 항산화 활성이21.78±0.68 μg/ml로 대조구인 ascorbic acid(66.49±0.56 μg/ml)보다 3배 정도 강한 활성을 나타내었다. Sage의 whole로부터 얻은 메탄올 추출물은 암세포에 세포독성을 가지고 있었으며, 치은섬유아세포에서는 독성이 거의 없는 것을 확인할 수있었다. Tyrosinase 저해활성을 검정한 결과, sage의 whole (56.23±0.21 μg/ml)과 root(63.36±0.69 μg/ml)로부터 얻은 추출물은 대조구인 arbutin(69.56±0.15 μg/ml)보다 높은 활성을 나타내었다. 이에 이들 식물들에 대해 추출용매 및 추출온도 등추출조건에 따른 최적 추출효율을 검토하고, in vivo 실험을 통해 생리활성 능력을 검정하는 등 보다 정밀한 연구가 필요할 것으로 사료된다.

자생 초본식물의 보존과 임간재배에 필요한 기초자료를 얻고자, 가리왕산과 중왕산 지역의 아고산대에 분포하는 천연활엽수림을 중심으로 주요 자생 초본식물의 분포와 입지인자를 조사하고 상호관련성을 분석하였다. 초본식물 분포조사에서는 136종의 초본식물이 확인되었으며, 개별꽃의 상대우점치가 10.8%로 가장 높게 나타났다. 그 밖에 주요 식물종은 벌깨덩굴, 현호색, 참나물, 대사초, 단풍취, 큰개별꽃, 얼레지, 곰취, 만주족도리풀, 피나물, 노루오줌 등 이었다. 노루귀와 단풍취; 참나물과 두메투구꽃; 두메투구꽃과 풀솜대; 벌깨덩굴과 큰개별꽃, 등의 종간에는 매우 높은 정의 상관이 인정되었다. 주요 식물종의 유집분석 결과, 7개의 그룹으로 나뉘어졌다. 주요 자원식물종이 선호하는 입지인자들이 밝혀졌다. 곰취, 단풍취, 박쥐나물, 얼레지, 참나물 등은 해발고가 높을수록 많이 분포하였고, 곰취는 토심이 깊을수록 많이 분포하는 것으로 나타났다. 얻어진 결과는 평창군 아고산 천연활엽수림의 보전과 주요 지원식물의 임간재배에 활용할 수 있을 것이라 사료된다.

This study was conducted to investigate the residue amount of harmful materials on the 153 commercial medical herbs in Gwangju area. It was performed using the GC-ECD, GC-NPD, GC-MSD and the LC-UVD,LC-FLD, LC-MSD to analyze 200 pesticides. The heavy metals were determined using a Mercury analyzer and AAS. The sulfur dioxides were analyzed by modified Monnier-Williams method. The residual pesticides were detected in 7samples, and were over MRLs (Maximum Risk Levels) in 2 samples (1.3%). The mean values of heavy metal contents (mg/kg) were Pb, 0.570; Cd, 0.081; As, 0.082; Hg, 0.0093. The measured values of Pb, As, Hg showed within MRLs. The excess samples of MRLs were 3 samples (changchul 2, cheongung 1) on Cd. The sulfur dioxides were over MRLs in 7 samples (4.6%), hwanggi and gugija. These results will be used to establish on the regulation of commercial medical herbs in Gwangju area.

A survey of total aflatoxin levels was conducted on 145 samples(carthamiflos, thujae semen, giycyrrhizae radix et rhizoma) collected in Yakyeang markets in Seoul. Aflatoxin levels were quantified by the immunoaffinity column clean-up method followed by performance liguid chromatography(HPLC)-fluorescence detector(FLD). Aflatoxins were found in 10(6.9%)samples including 5 Arecae semen, 4 Thujae semen, 1 Zizyphi semen with a range of 0.45~79.15 μg/kg. Generally These results show that the contamination level of aflatoxins in Herb Medicines consumed in Korea is high compared with the standard in Korea Herb Medicine Code(10 μg/kg as aflatoxin B1). It is considered that aflatoxin concentration was increased in herb medicines during a storage and drying in herb medicines examined

The acricidal potency of some essential oils can be applied to the control of house dust mites. But it is more effective keeping the house dust mites away than killing them because they remain as allergens even after death. The most efficient repellent concentration of lavender and tea tree in 10㎕ against house dust mites (Dermatophagoides farinae and D. pteronyssinus) were experimented by direct contact bioassay method at various doses ; 2.5, 1.25, 0.625, and 0.3125% and at vairous exposure times ; 15, 30, 60, 120, and 180 minutes. The most effective dose of essential oils (lavender and tea tree) against house dust were 0.625% and the repellent effectiveness at the mowt effective dose of 0.625% were 95.6% for lavander and 96.2% for tea tree, respectively. These results suggest that lavender and tee tree can be effectively used in the control of house dust mites.

The insecticidal potency of some essential oils have been suggested that they may find an application in the control of house dust mites. We compared the repellent effect of four essential oils ; lemon grass (Cymbopogon nardus), lavender(Lavandula vera), rosemary(Rosmarinus officinalis) and ylang ylang(Cananga odorata). The oils were exposed at different doses(0.1, 0.05, 0.025, 0.0125, and 0.00652 ㎕/㎠) and different times(1, 2, 3, 4, 5 hours) on house dust mites(Dermatophagoides farinae and D. pteronyssinus). The repellent effects(%) of essential oils that were exposed at 0.1 ㎕/㎠ and for 1 hours against house dust mites were lemon grass(63.3%) and rosemary(61%), lavender(40%), ylang ylang(40%) in the order named.

The cosmetic and toiletries are necessary health care & household for common life. However we need antiseptic which is effecting harmlessly to the human body. There are propolis, Lavender, Lemon, essential oil in the natural antiseptic materials. This work proceeded design Natural-antiseptic system with three materials as above-mentioned. Natural-antiseptic system was accomplished with propolis (2%), Lavender essential oil (0.3%), Lemon essential oil (0.3%) safety out of Polysorbate 20 (0.5%), Polysorbate 80 (0.5%), PEG (60) hydrogenated castor oil (0.45%), ethanol (5%). The antimicrobial test was experimented on E. coli and Bacillus stearothermophilus. In this antimicrobial test, we found that the effect of antisepsis against E. coli and Bacillus stearothermophilus with propolis 0.3%, Lavender essential oil 0.045% and Lemon essential oil 0.045% was improved. Therefore could expect Natural-antiseptic system product for moisturizing skin toner for face, nourishing essence and wet tissue for clean other things.

This study examined the consumptive perceptions of herb added breads and cookies with 436 male and 444 female university students. Also this study tested sensory preference of baguette. The results of this study were as follows: First, the intention rate of herb added breads and cookies were high with 85.5% (N=752) and female was higher with 89.6% than male with 81.2%. In terms of consumption prospect, 48.0% of them responded with increasing, 45.6% of them responded quite positively with 'average'. The 4.4% of them responded with decreasing. There was significant difference about consumption between male and female. Second, 45.8% of them responded herb added breads and cookies consumption rate will be increased due to consumers are seeking new tastes. The 59.0% of them responded consumption rate will be decreased due to they are not familiar with new taste. Third, in terms of sensory preference of rosemary added baguette, appearance, flavor, taste, overall acceptability was high with 2% added group. The texture was high with 1% added group. The appearance and flavor preference of mint added baguette breads was high with 1% added group. The taste, texture, and overall acceptability was high with control group. Fourth, while male preferred rosemary 1% on appearance, flavor, taste, texture, overall acceptability, female preferred rosemary 2% on appearance, flavor, taste, overall acceptability. Both of them evaluated mint 4% as the least preferred.

In order to search for anti -melanin formation agents from Korean medicinal herbs, we selected 21 Korean medicinal herbs, based on a review of Korean traditional medicine books and the recommendations of Korean traditional medical doctors. We tested for i

곰취를 비롯한 15종의 자생 초본류의 종자특성인 종자수득율, 천립중, 종자길이와 폭을 측정하였으며, 6종의 자생 초본류의 종자를 가을파종으로 임간 발아시험을 하였다. 참취를 비롯한 13종의 자생 초본류 종자가 7mm미만 의 길이와 3mm미만의 폭을 지닌 세립종자들이었다. 곰취를 비롯한 10종의 자생 초본류의 종자수득율이 5% 미만으로 매우 낮은 편이었다. 참나물을 비롯한 5종의 자생초본 발아율은 생육지간 통계적 유의차가 인정되었다. 평균 발아율은 참당귀가 47.00%로 가장 높았고, 곰취가 38.25%, 참나물이 30.88% 독활이 15.75%, 고려엉겅퀴가 10.00% 였고, 참취가 9.32%로 가장 낮았다.

Quality stability of the herb pill coated with edible oils containing rosemary was investigated. Herb pills were made of herb powders such as Panax ginseng, Cinnamomum cassia, Lycium chinense, Zyzyphus jujuba and Zingiber officinale. Rapeseed oil and lubriol were used as edible coating oil. After herb pills coated with edible oils with or without rosemary were stored at 40℃ for 180 days, the microbial viable cell counts and peroxide values(POV) of the herb pill were investigated. After 180 day storage, POVs of herb pills with only rapeseed oil or lubriol were 0.51 and 0.49 meq/kg, respectively. However, when rosemary was added in herb pills the POVs were decreased to 0.30 and 0.39 meq/kg, respectively. The addition of rosemary to the rapeseed oil and lubriol tended to decrease the microbial viable cell counts of the herb pill. The microbial viable cell counts of rapeseed oil and lubriol were 940 and 820CFU/g, respectively after 180 days of storage. However, these levels were suppressed to 720 and 640CFU/g by the resemary addition. On the other hand, the ginseng saponin content of herb pills was not affected by the rosemary addition during storage.