본 실험은 나이가 다른 잣나무조림지에서의 토중 톡톡이 군집의 특성을 조사하기 위하여 수행하였다. 조림후 1~2년된 지역에서 11~12년된 지역을 1986년 7월부터 1987년 6월까지 조사한 결과는 다음과 같다. 전체적인 톡톡이군집?조는 각종에 따라 심한 집중분포현상을 나타내고 있고, 동일종도 지역에 따라 다른 분포양식을 나타낸다. 조림연도별 군집구조는 조림연도가 오랠수록 종다양도가 높아지고, 밀도와 종자도 안정되어 있었다. 조림후 2년이 지난 1985년조림지에서는 동경사면인 I구가 서면사면인 II구보다 종다양도가 높았다. 계절적 군집구조는 온도가 높은 8월과 유기물함량이 가장 많은 10월에 밀도와 종류가 높은데 이는 톡톡이군의 활동이 온도, 습도, 유기물량에 형향을 받는 것으로 생각된다.

The study was investigated in vegetation and environmental factors of Thuja koraiensis habitats where provided for ecological evidence data for conservation and management. Study plots were located at altitudes of 1,000∼1,588 m with inclinations of 10∼30°. A total of 150 taxa was identified 22 quadrates in 6 study sites. The highest basal area was analyzed in Mt. Gariwangsan 49.98 ㎡/㏊ with the lowest Mt. Hwaaksan 3.46 ㎡/㏊. Thuja koraiensis forest was classified into two communities; Abeis nephrolepis-Taxus cuspidata community, Betula ermanii-Thuja koraiensis community. As a result of Multi-Response Permutation Procedures test, there was significant difference among the communities.

Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via α-glucosidase inhibitory assays. The mode of inhibition by AKEs on α-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent α-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had α-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant α-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36㎎/㎖ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10㎎/㎖. It can therefore be used as a source of therapeutic agents to treat diabetes patients.

Background : Medicinal wild vegetables refer to wild medicinal plants whose aerial parts are edible. Recent researches for developing a functional food product from medicinal wild vegetables have been actively reported. The objective of this study is to test anti-diabetic activity of 2 medicinal wild vegetables, Allium victorialis and Aster koraiensis. Methods and Results : The medicinal wild vegetables were extracted using water and ethanol. Several medicinal wild vegetables were screened for anti-diabetic activity using α-glucosidase inhibitor screening test (colorimetric). It utilizes the ability of an active α-glucosidase to cleave a synthetic substrate and releasing a chromophore (OD 410 ㎚). In the presence of an α-glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings. The α-glucosidase inhibitory activity of acarbose was compared with wild vegetables extracts at 1 ㎎/㎖. And A. victorialis and A. koraiensis extracts were selected. α-glucosidase inhibitory activities of A. victorialis and A. koraiensis extracts were confirmed in various concentration. Conclusion : These results suggest that A. victorialis and A. koraiensis could be good candidates for anti-diabetic material.

Background : Collagen and elastin contribute to form a network under the epidermis and reduce the wrinkles. Collagen and elastin are degraded by collagenase and elastase. Therefore, inhibition of collagenase and elastase activity could be an excellent method for anti-wrinkle. Pinus Koraiensis leaves contain various flavonoid compounds such as quercetin and kaempferol. According to previous reports, kaempferol has increases procollagen synthesis and inhibitory effect on MMP-1 activity. This study was performed to investigate the anti-wrinkle effect of Pinus Koraiensis leaves extract and fractions. Methods and Results : The active components of Pinus Koraiensis leaves extracts and fractions were determined. Total phenol and total flavonoid contents measured using the Folin-Ciocalteu reagent and according with aluminum chloride colormetric method. The kaempferol content analysis using HPLC system. The total phenol, flavonoid and kaempferol contents of ethyl acetate fraction was the highest. The antioxidant activity measured in according with DPPH, ABTS and RP assay. Pinus Koraiensis leaves extract and factions were the highest ethyl acetate fraction in antioxidant activity. The inhibitory activity on collagenase and elastase of Pinus Koraiensis leaves extract and factions were determined. The results of inhibitory activity on enzymes associated with wrinkle formation was the highest of n-butanol fraction, the next higher was 80% ethanol extract. As a result of using MMP-1 content and procollagen type-I C-peptide content assay kit, the anti-wrinkle effect of n-hexane fraction was highest. Conclusion : These results indicate that Pinus Koraiensis extract may help anti-wrinkl

This study was conducted to elucidate the photosynthetic response to the environment and establish optimum cultivation conditions for the Korean endemic plant, Aster koraiensis. Photosynthetic characteristics according to growth stage, light, CO2, and soil water potential were investigated. During the first year of transplanting, photosynthetic rates were drastically increased until June, after which they slowly declined, During the second year, photosynthetic rates declined throughout the entire growth period. The highest level of light compensation point was shown the early growth stage. Photosynthetic rates affected by intercellular CO2 concentration were maintained or decreased over the CO2 saturation point. The lowest CO2 compensation point was 16.1 μmol･mol -1 during March. The morphological changes of leaves were observed due to shading with chlorophyll contents increasing. Photosynthetic rates were higher at 0% and 50% shading treatments than at 75%. There were rarely any morphological changes of leaves due to soil moisture, however, changes to leaf compactness were observed. Photosynthetic rate, apparent quantum yield, and respiration rate increased, whereas water use efficiency decreased over -25 kPa of soil moisture.

Background : Scrophularia koraiensis is a herbaceous perennials belonging to the family Scrophulariaceae. This study was carried out to survey the effect of soil types on growth characteristics in Scrophularia koraiensis seedling. Methods and Results : Seeds were collected from plants growing in the Mt. Kariwang Jeongseon, Gangwon-do in September 2014, and they were sown to 128 cell plug tray filled with Peatmoss (TKS-2) soil at March, 2015. The experiment was performed with four different soil types (Peatmoss, Peatmoss + Perlite, Peatmoss + Granite soil, Commercial soil). According to the experiment, stem diameter was the highest under commercial soil. The leaf width and length were the highest under commercial soil. Main root diameter and lengh were the highest under commercial soil. Conclusion : According to the results, Scrophularia koraiensis seedling showed the highest growth in commercial soil.

Background : Scrophularia koraiensis is a herbaceous perennials belonging to the family Scrophulariaceae. This study was carried out to survey the effect of LED on root develop characteristics in Scrophularia koraiensis seedling. Methods and Results : Seeds were collected from plants growing in the Mt. Kariwang Jeongseon, Gangwon-do in September 2014, and they were sown to 128 cell plug tray filled with Peatmoss (TKS-2) soil at March, 2015. The experiment was performed with three different LED (Blue, Red, Blue + Red) at July, 2015. Morphological characteristics of root (total root length, root projet area, root surface area, root diameter and root volume) were analyzed with WinRHIZO software. Seedling root growth of scrophularia koraiensis was surveyed to be the highest at the Blue + Red LED in all measuring. Total root length was measured high in the order of Blue + Red, Red, Blue LED. Conclusion : According to the results, Scrophularia koraiensis seedling showed the highest root growth in Blue + Red LED.

본 연구는 잣나무의 구과 착과량에 따른 구과 및 종자특성의 변화 양상을 구명함으로써 잣나무 채종원의 효율적 관리방안을 도출하기 위해 수행되었다. 이를 위해 2007년 충주 소재 잣나무 채종원에서 조사를 실시하였는데, 전체 조사목 중 96.3%가 착과되었으며 본 당 구과수는 평균 27.4개였다. 본 당 구과 착과수에 따라 8개의 계급으로 나누어 분석을 했는데, 구과 착과수가 많아질수록 구과길이, 구과폭, 구과무게가 감소하는 것으로 나타났다. 또한 구과 착과수가 많아질수록 임성인편수, 종자생산능력, 충실종자율, 종자무게, 종자효율은 감소하고 둘째해 고사배주율은 다소 증가하는 경향을 나타냈다. 이러한 결과를 볼 때 종자결실이 풍년으로 구과가 다량 착과되는 해에는 평년에 비해 종자의 균일성 및 품질이 저하될 가능성이 높다고 판단된다. 특히, 잣나무는 구과와 종자의 크기가 크고 무거울수록 포지와 조림지에서의 생장이 좋다는 보고가 있는 점을 감안할 때, 결실 풍년으로 인해 종자의 평균적인 크기와 무게가 감소되는 경우 향후 조림지에서의 유묘생장도 저조할 가능성이 있다. 따라서, 이러한 문제점을 해결하기 위해 결실이 풍년인 해에는 착과량을 인위적으로 적정수준으로 조절하거나 종자 정선 시 평년 수준의 종자크기 또는 무게를 가진 것들을 선별하여 공급하는 방안 등 다각적인 대처방안을 검토할 필요가 있을 것으로 생각된다.

본 논문은 잣나무 숲 속에 존재하는 피톤치드 농도와 온도, 풍속의 상관성을 밝히고자 시도한 연구이다. 이를 위해 2011년 5월부터 11월까지 약 7개월에 걸쳐 매월 2회씩 조사하였으며 매회 하루 중 일출, 남중, 일몰 세 차례 측정하였다. 온도, 풍속과 피톤치드의 상관성에 대해 다음과 같은 결론을 얻었다. 1) 잣나무 숲에서 일출 시간에는 α-Pinene 검출량이 가장 많았으며, 남중 시간에는 Benzaldehyde, 일몰 시간에는 Camphene이 가장 많이 검출되었다. 2) 날짜별 변화에서 α-Pinene, Camphene, β-Pinene, Benz-aldehyde 4가지 물질 모두 온도가 낮아질수록 농도가 감소하는 경향을 보였다. 특히 7∼8월을 정점으로 뚜렷한 감소 경향을 보였다. 3) α-Pinene, Camphene, β-Pinene, Benzaldehyde는 대체로 일출 시간에 농도가 높은 경향을 보이며 온도가 높은 남중 보다는 일몰 시간에 더 높은 추세를 나타낸다. β-Pinene은 하루 중 온도가 높은 남중 시간에 가장 낮은 농도를 보이고, 비교적 온도가 낮은 일출과 일몰 시간에 높게 나타났다. 4) 본 연구는 잣나무 숲 대기 중에 축적된 피톤치드 농도를 측정하는 것이기 때문에 측정 전 기상상태의 변화(강풍, 강수 등)가 있을 경우 농도가 낮게 측정됨을 알 수 있었다. 이것은 비나 바람이 강한 날 이후에는 산림욕의 효과가 다소 적어질 수 있음을 시사한다. 5) 풍속이 약할수록 α-Pinene, Camphene, β-Pinene, Benzaldehyde 검출되는 양이 증가하고 풍속이 강할수록 검출되는 양이 감소하는 경향을 보였다. 본 연구를 진행하는 동안 측정 현장에서 완벽한 자연상태의 포집에 어려움이 있었다. 향후 피톤치드 포집 방법, 지리적 위치, 지형적 특징 등이 피톤치드 농도 분포에 어떠한 영향을 끼치는지에 대한 연구가 필요할 것이다. 본 연구에서 얻어진 기초자료들은 잣나무 숲에서의 치유효과를 검증하려는 연구자들에게 유용하게 활용 될 수 있으며 치유의 숲에 대한 일반인들의 인식을 높이는데 사용되어 질 수 있다고 사료된다.

본 연구는 자생 벌개미취를 이용하여 다양한 토양에 오염된 중금속의 정화 가능성을 분석하기 위하여 시행되었다. 중금속으로 오염된 논, 밭 및 수림지에 벌개미취를 식재하여 8주간 재배한 후 생육 및 중금속 흡수능을 분석하였다. 벌개미취는 중금속으로 오염된 논, 밭 및 수림지에서 8주 동안 재배한 결과 벌개미취는 중금속 오염 토양에서도 생육이 우수한 것으로 나타나 중금속 내성이 있는 것으로 생각되었다. 또한 중금속에 오염된 다양한 토양에서 벌개미취가 흡수한 비소, 카드뮴, 구리, 납 및 아연의 함량을 분석한 결과, 벌개미취는 5종의 중금속을 모두 흡수하는 것으로 나타났으나, 토양 내 중금속의 함량 및 토성에 따라 흡수능이 각기 다르게 나타났다. 비소, 카드뮴 및 구리의 경우에는 토양 내 중금속 함량에 영향을 많이 받는 것으로 나타났으며, 토양 내 중금속의 함량이 높을수록 흡수능이 증가되었다. 납은 토양 내 중금속의 함량 보다는 토성에 의하여 흡수능이 달라지는 경향을 보였으며, 사질토인 수림지에서 흡수능이 가장 증가되었다. 아연의 흡수능은 토양 내 아연의 함량과 토성이 모두 영향을 미쳤는데, 고농도의 중금속으로 오염된 논과 밭에서 재배하였을 때 아연의 흡수능이 증가하는 것으로 나타났다. 연구의 결과, 벌개미취는 중금속에 대한 내성이 있으며, 다양한 중금속에 대한 흡수능이 우수하므로 중금속으로 오염된 여러 종류의 토양에 적용 가능한 경관식물 소재로 이용할 수 있을 것으로 생각되었다.

본 연구에서는 잣나무 잎 추출물의 항균, 항산화 및 성분 분석에 관한 연구를 수행하였다. 피부 상재균에 대한 항균활성 측정결과, Propionibacterium acnes (P. acnes), Staphylococcus aureus (S. aureus), Pityrosporum ovale (P. ovale), 및 Escherichia coli (E. coli)에 대한 ethyl acetate 분획의 MIC는 각각 0.06 %, 0.25 %, 0.13 %, 0.50 %로 나타났으며, P. acnes, P. ovale 및 S. aureus에서 큰 항균활성을 나타내었다. 잣나무 잎 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC50)은 aglycone 분획에서 22.93 μg/mL으로 나타났다. Luminol-의존성 화학발광법을 이용한 Fe3+-EDTA/H2O2계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 잣나무 잎 추출물의 총 항산화능(OSC50)은 50 % ethanol extract 분획에서 0.70 μg/mL, ethyl acetate 분획 및 aglycone 분획이 각각 1.04 및 1.43 μg/mL으로 매우 큰 항산화능을 보였다. Rose-bengal로 증감된 사람 적혈구의 광용혈 실험에서 추출물의 세포보호 효과를 측정하였다. 잣나무 잎 추출물 분획들은 농도 의존적(5 ~ 50 μg/mL)으로 세포보호효과를 나타내었다. 잣나무 잎 추출물의 가수분해로 얻은 ethyl acetate 분획은 TLC (PK-4, PK-6)와 HPLC (peak 1, peak 2)에서 2개의 주성분으로 나타났다. 이 성분들은 LC/ESI-MS/MS를 통해서 PK-6는 kaempferol-3-O-glucoside (astragalin)로, PK-4는 kaempferol-3-O-arabinoside (juglanin)로 확인되었다. 이상의 결과들은 잣나무 잎 추출물이 ROS를 소광시키거나 소거함으로써, 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리키며, 잣나무 잎 성분 분석, 그리고 피부 상재균에 대한 항균작용으로부터 항산화, 항노화 및 항균성 화장품 소재로서의 응용 가능성이 있음을 확인하였다.

This study was carried out in order to estimate site indices and to investigate productivity of Pinus koraiensis in unstocked land by the relationship between site index and physico-chemical properties of forest soil in stocked land. In Pinus koraiensis stand 83 nationwide sites were selected and site index model were estimated with 14 items of physico-chemical properties respectively obtained from A and B horizon of forest soil in each site. According to the relationship between soil properties and site index, it was found that the regressed models by soil properties fitted well in both site production prediction of soil A and B horizons. As a result of these two regressions, significant correlation was found between site index and Exch. Ca++, O.M., Clay, T.N., and Sand in A horizon, while Avail. P2O5, Base Sat., C.E.C., Exch. Mg++, and T.N. and site index have grater correlation in B horizon. In conclusion, these results will provide not only the important criteria for establishment of management forest plan in unstocked land, but also the useful guidance for selecting the suitable sites and trees.

Estimates of genetic gain (in volume growth) and diversity (expressed as status number, Ns) were determined in a clonal seed orchard of Pinus koraiensis. The genetic thinning was based on clonal breeding values (represented by general combining ability) obtained from progeny tests, clonal fertility estimated by strobilus production, and clonal size variation determined by the ramet numbers per clone. Parental GCA values for volume growth were calculated, based on height and diameter at breast height measured from field trials. Clonal fertility was estimated from the assessments of strobilus production over twelve years from 1991 to 2003, and used for the calculation of status number. There are 179 clones and 5,268 ramets in 12ha area of P. koraiensis clonal seed orchard. Genetic gain and diversity estimates were determined under assumptions of 30% pollen contamination and inferior genetic value of contaminating pollen. Genetic gain increased as thinning rates were set from 10% to 60%. However, for the higher thinning intensities, the increase of genetic gain was not remarkable. Genetic thinning by means of truncation selection resulted in a greater genetic gain but a large decrease in status number. Status number was represented around 40 clones for 10% through 60% thinning intensities, but for the higher thinning intensities, it was a bit fluctuated. Based on the present results, it could be concluded that thinning rate should not be stronger than 60% to optimize genetic gain while conserving genetic diversity. Consequently 50% or 60% thinning rate might be appropriate for genetic thinning in the clonal seed orchard of P. koraiensis. The effect of pollen contamination on the genetic gain and the consequence of genetic thinning for seed production in the clonal seed orchard, and seed orchard management scheme were also discussed.

본 연구는 잣나무의 영양생장과 생식생장간의 상관관계를 구명하여 잣나무 채종원의 효율적 관리방안, 임목개량을 위한 선발기준 설정 및 우량품종 육성 등 금후 선발육종 방안을 제시하고자 하였다. 잣나무의 영양생장 분석을 위하여 차대검정 결과로부터 재적생장에 대한 일반조합능력을 추정하였으며, 재적생장은 수령 8년, 10년, 15년 및 20년 때의 수고 및 흉고직경 생장으로부터 산출하였고 이를 10년 동안의 개화량 성적과 비교·분석하였다. 그 결과 재적생장에 대

In order to prevent cones from being damaged by cone insects, Gravitarmata margarotana and Dioryctria abietella, sprinkler system was installed on the Pinus koraiensis Seed Orchard. Such a test was conducted at Chung-ju, Chungcheong buk-do, Korea in 2002. Insectides of Diflubenzuron 25% WP ×2,500 and Cyfluthrin 2%EC × 1,000 were sprayed at fifteen-day and twenty-day intervals during the period of insects adult occurrence by sprinkler system. The Gravitarmata margarotana generally emerged for about 53 days from middle April to early June, and peak emergence was 10 days from April 20 to April 30. Dioryctria abietella generally emerged for about 108 days from late April to early August, and peak emergence was 10 days from May 20 to May 30. Damage rate of cones were 8.7~9.7% and 10.9~12.7% on average with crown insecticide spraying with Cyfluthrin and Diflubenzuron, while 67.5% when not treated. Control effectiveness of this insecticide spraying was 85.6~87.1% and 83.9%와 81.2%, respectively. When it considers the forest environment, it is recommended diflubenzuron which is low toxic pesticide. In conclusion, effectual times and number of diflubenzuron 25% WP application with sprinkler system against cone insects were from early May through late August and six times at twenty-day interval.