The aim of this study was to isolate and identify marine bacterium with anti-methicillinresistant Staphylococcus aureus (MRSA) activity, and to purify the anti-MRSA compound, as well as to determine its activity and synergistic effects. Among the marine bacteria isolated in this study, the YJ-1 isolate had the strongest anti-MRSA activity. The YJ-1 isolate was identified on the basis of its biochemical characteristics and an analysis of 16S rRNA gene sequences. The YJ-1 isolate showed over 99.2% homology with Pseudomonas stutzeri, and was designated as a Pseudomonas sp. YJ-1. The optimal culture conditions were 25℃ and initial pH 7.0. For the purification of the anti-MRSA compounds, the YJ-1 was cultured in Pa PES-II medium, and the culture filtrates were extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction was separated by a C18 ODS column, silica gel chromatography and a reverse phase HPLC, to yield three anti-MRSA agents, the MR1, MR2, and MR3 compounds. When the MR1 compound of 250 μg mL-1 concentration was applied to the MRSA cells, over 95% of bacterial cells was killed within 48 hr. Compared with vancomycin and ampicillin, the MR1 compound showed significant anti-MRSA activity. In addition, the anti-MRSA activity was increased by dose and time dependent manners. Furthermore, the combination of an MR1 compound with vancomycin produced a more rapid decrease in the MRSA cells than did the MR1 compound alone. Taken together, our results suggest that the Pseudomonas sp. YJ-1 and its anti-MRSA compounds could be employed as a natural antibacterial agent in MRSA infections.

본 연구는 마치종 황색 알곡 품종으로 등록된 강일옥 옥수수 알곡의 처리별 시료 및 속대의 일반성분, 생리활성 검정, 색소 함량을 분석하여 기초자료로 제공하고자 수행되었다. 강일옥 옥수수를 알곡, 거피된 알곡, 알곡 껍질 및 속대로 분리하여 일반성분 분석 결과, 수분함량은 거피된 알곡이 12.04%로 가장 높았고 알곡 껍질의 조회분, 조지방, 조단백질 함량은 각각 3.19%, 8.59%, 12.01%로 강일옥 옥수수 처리별 시료 중 가장 높은 함량을 나타내었다. 강일옥 옥수수 처리별 시료의 무기성분 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 철, 망간 및 인의 6종이 검출되었으며 공통적으로 검출된 주요 무기성분은 칼륨과 인이었다. 강일옥 옥수수 속대의 칼륨 함량이 114.33 mg/ 100 g으로 가장 높았으며 알곡 껍질의 인 함량이 2412.40 mg/100 g으로 가장 높게 측정되었다. 강일옥 옥수수 처리 별 시료의 항산화 활성 검정 결과, 강일옥 옥수수 속대 추 출물의 DPPH radical 소거능은 93.05%, ABTS radical 소 거능은 79.88%로 처리별 시료 중 가장 높게 측정되었으며 알곡, 거피된 알곡 및 알곡껍질 추출물의 DPPH 소거 능은 모두 70% 이상으로 우수한 항산화활성을 나타내었다. 강일옥 옥수수 처리별 시료의 항염 활성 검정 결과, 강일옥 옥수수 처리별 시료 추출물은 Raw264.7 대식세포 의 생존율에 영향을 미치지 않은 것으로 나타났으며 NO 생성량은 모든 시료 처리군에서 LPS 단독 처리군(con(+)) 에 비하여 유의적으로 NO 생성을 억제 하는 것으로 나타났다. 강일옥 옥수수 처리별 건조분말시료의 카로티노이드 색소 함량을 분석한 결과, 모든 시료에서 β-carotene은 검출되지 않았고, lutein과 zeaxantin은 알곡과 알곡 껍질 에서 검출되었으며 거피된 알곡과 속대에서는 검출되지 않았다. 강일옥 옥수수 알곡의 lutein과 zeaxantin의 함량은 각각 115.30 μg/100 g, 50.92 μg/100 g이었으며 알곡껍질의 lutein과 zeaxantin의 함량은 각각 37.98 μg/100 g, 19.27 μg/100 g이었다.

Sorghum is a rich source of various phytochemicals including phenolic compounds that have potential to significantly benefit human health. Phytochemical production may be induced not only by genotype but also by a number of environmental factors including temperature and an amount of sunshine. The objective of this study was to determine the effect of planting dates and harvesting stages on the quality traits of ‘Donganme,’ a grain sorghum variety developed to produce high antioxidants. ‘Donganme’ was planted in three locations on four dates from early May to early July. Each planted fraction was harvested 35, 40, 45, 50, and 55 days after the head shooting date, respectively. The results showed that significant differences existed between the growth period and content of polyphenol and flavonoid. The sorghum harvested at 35 days after head shooting had the higher polyphenol and flavonoid content in all planting times and the tannin content was increased as the planting date was delayed. So the sorghum grown in late planting dates (July 5) had highest polyphenol content (2.07 gGAE/100g) at early harvesting (35 days) while late planting gave the low grain yield (1.3 ton/ha). Polyphenol index to average temperature during 35 and 45 days after heading of ‘Donganme’ showed that total polyphenol contents were decreased by 5.2% and 4.4%, respectively, with increasing average temperature of 1oC. And high correlation (R=0.85) was observed between accumulated temperature and the amount of polyphenol of ‘Donganme’, confirming the major contribution of the quality traits is temperature. To produce high quality grain from sorghum the relation between the yield and nutrition components must be considered simultaneously (i.e., Sorghum produced the highest polyphenol and flavonoid when planted in June 15 and harvested at 40 to 45 days after head shooting .

In this study, we investigate the potential use of persimmon peels (PP) in mushroom culture medium for the production of functional mushrooms. Pleurotus eryngii was cultivated in medium supplemented with PP (SMPP) at the following concentrations: 0% SMPP (control), 5% SMPP, 10% SMPP, 15% SMPP, 20% SMPP, or 30% SMPP. The total polyphenol content, DPPH radical scavenging ability, ABTS cation scavenging ability, and reducing power of P. eryngii cultivated in SMPP were investigated. P. eryngii cultured in 20% SMPP produced the highest values for all four measurements. Total polyphenol content, DPPH radical scavenging activity, ABTS cation scavenging ability, and reducing power all increased upon the addition of PP. Based on our results, we can conclude that persimmon peels are a highly valuable supplement for functional mushroom culture medium.

신체활동은 노화로 의한 인지기능 저하에 긍정적 영향을 미친다. 하지만 신체활동 참여가 정서인지기능에 미치는 영향을 과학적으로 밝혀 낸 연구는 드물다. 따라서 본 연구의 목적은 청년기와 노년기 성인의 정서인지 기능에 신체활동 참여가 어떠한 영향을 미치는지를 뇌 과학적으로 연구하는 것이다. 연구의 피험자는 D 광역시 성인 남성 60명으로 선정하였으며, 청년 운동 집단(25∼35살), 청년 비 운동 집단(26∼35살), 노년 운동 집단(60∼70살), 노년 비 운동 집단(60∼70살) 4개의 집단으로 구성하였다. 실험도구는 EEG 측정 장비와 국제정서사진체계(International Affective Picture System: IAPS)를 사용하였다. 본 연구의 실험과제는 사진으로 제시하는 세 가지 정서자극(긍정적, 중립, 부정적)에 적합한 버튼을 눌러 정서적인지 검사를 실시하였다. EEG 측정은 8개(Fp1, Fp2, Fz, C3, C4, Cz, T3, T4)의 영역을 측정하였다. 통계처리는 4(집단)×3(자극)×8(영역)에 대한 삼원분산분석을 실시하였다. 결과: 본 연구 결과, 반응시간과 반응정확률 그리고 P3 잠재기는 집단간 주효과가 나타났다. 그리고 P3 진폭은 집단과 자극간 상호작용이 나타났다. 결론: 신체활동은 청년층과 노년층의 정서적 인지기능에 긍정적인 영향을 미치는 것으로 나타났다.

According to the nuclear safety act, the enforcement regulations and the notification of the atomic energy commission, a preliminary decommissioning plan must be submitted at all domestic nuclear facilities. In accordance with this preliminary decommissioning plan, it is required to prepare eleven items from the outline of the decommissioning plan of the nuclear facility to the fire protection. Currently, the nuclear fuel cycle facility operated by the Korea Atomic Energy Research Institute (KAERI) consists of a radioactive waste form test facility (RWFTF), a post irradiation examination facility (PIEF), a radioactive waste treatment facility (RWTF), and a radioactive waste storage facility (RWSF). The decommissioning strategies, decommissioning methods and dismantling activities of these nuclear facilities are described in this paper. The scope of decommissioning, the dismantling method, the final conditions of the site, the management of radioactive waste, and the cost of decommissioning are established in the decommissioning strategy. The decommissioning schedule, work order, basic principle and technical feasibility are determined at the method of decommissioning. The disinfection techniques and activity plans for facilities and sites contaminated with radioactive materials are described at the dismantling activity. Therefore, this paper describes the concept of decommissioning of the nuclear fuel cycle facilities and prepares a preliminary decommissioning plan to be prepared afterwards.

β-glucosidase is an enzyme that hydrolyzes glycosidic bonds to generate terminal non-reducing residues in β-D-glucosides and oligosaccharides. These enzymes are used to degrade the plant cell walls of plant biomass. The 170 lactic acid bacteria isolated from beachu kimchi were examined for their β-glucosidase activity(E.C. 3.2.1.21.). Among the tested lactic acid bacteria, 48 strains utilized cellobiose as a carbon source. The highest β-glucosidase activity was 0.0537±0.0021(U/mg protein) in MSE129. MSE129 was identified to be Weissella koreenisis using 16S rRNA gene sequence analysis.

Licorice and rosemary contain a number of natural phenolic compounds which have a strong antibacterial and antioxidant activity. Ulleungdo stonecrop (US, scientific name: Sedum takesimense Nakai), indigenous island, is also known as a good antibiotic agent and skin whitening agent and is regarded as a promising materials in cosmetics field. Although the antibacterial activity of each material have been reported, little is known about the effect of using them together. Therefore, the purpose of this study was to investigate the synergistic effect on antibacterial activity when licorice and rosemary extract and US were used together. Since licorice and rosemary are lipid soluble materials and US is water soluble material, oil in water (O/W) emulsion system was used for this study. To manufacture O/W emulsions containing three kinds materials, first licorice and rosemary extracts and US was dispersed in soy bean oil and phosphate buffer (pH7) respectively. Decaglyceryl laurate was used as an emulsifier. There was slight decrease (-0.09~-0.46 Δlog) in the number of bacteria when O/W emulsion containing 300ppm of each licorice and rosemary extracts was mixed into B.subtilis. However, adding US (2,700ppm) into aqueous phase caused the rapid decrease in the amount of bacteria to -1.71 ~ -2.96 Δlog which were much higher decrement than US only in normal emulsion (-1.64 Δlog). In particular, there was a noticeable synergistic effect when rosemary extract and US was used together. The results obtained from this study could provide useful information for utilizing licorice and rosemary extracts and US as functional materials in the food and cosmetic areas.

Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. In this study, glucansucrase-producing lactic acid bacteria strain was isolated from the fermented foods collected in traditional markets, and the optimum conditions for the oligosaccharide production were investigated. The strain CCK940 isolated from Chinese cabbage kimchi was selected as an oligosaccharide-producing strain due to its high glucansucrase activity, with 918.2 mU/mL, and identified as Leuconostoc lactis. The optimum conditions for the production of oligosaccharides using Leu. lactis CCK940 were to adjust the initial pH to 6.0, add 5% (w/v) sucrose and 10% (w/v) maltose as a donor and acceptor molecules, respectively, and feed 5% (w/v) sucrose at 4 and 8 h of cultivation. When Leu. lactis CCK940 was cultured for 12 h at optimum conditions, at least four oligosaccharides with a polymerization degree of 2-4 were produced.

Background: The serratus anterior (SA) muscle is one of the important muscles in the upward rotation of the scapula when the arm is raised. Insufficient muscle activity of the SA can cause deformation of the shoulder rhythm resulting in shoulder pathology. Objects: This study intends to compare SA and upper trapezius (UT) activity during the conventional wall-slide and push-up plus exercises for SA muscle strengthening and the scapular upward rotation (SUR) exercise. Methods: A total of 30 subjects participated in this study, and we measured the muscle activity of the SA and UT muscles during the wall-slide, push-up plus and SUR exercises. The one-way repeated ANOVA was used to compare SA and UT muscle activities during the 3 exercises. Results: During the SUR exercise, SA muscle activity was 79.88% maximum voluntary isometric contraction (MVIC), which was significantly higher than its activity during the other 2 exercises. The UT muscle activity was 47.53 %MVIC during the SUR exercise, indicating a significantly higher UT muscle activity than during the other 2 exercises. Conclusion: These findings suggest that the SUR exercise can maximize SA muscle activity to strengthen the SA while keeping UT muscle activity at an appropriate level.