본 연구는 육계사육에서 횃대의 제공효과와 최적 두께를 구명하기 위해 진행하였다. 12일령 육계(RossⅹRoss) 288수를 24개의 독립된 공간(7.3 수/m2)에 균등한 평균 체중으로 분배하여 42일령까지 사양실험을 실시하였다. 그룹은 일반 평사사육(대조구)과 3개의 횃대 처리구(두께: 3, 4 및 5 cm)인 총 4개로 구성하였으며, 각 그룹은 6반복과 반복당 12수씩으로 배치하였다. 각 반복 별로 횃대의 길이는 1.3 m (0.11 m/수)로서 높이 15 cm에 설치하였다. 조사항목으로 육계의 생산성, footpad dermatitis (FPD)과 hock burn (HB) 지표 및 횃대이용성을 확인하였다. 본 연구결과, 육계사육에서 횃대의 유무와 두께에 따른 체중, 증체량, 사료섭취량 및 사료요구율은 통계적 차이가 없었다. FPD는 처리구 간 유의적 차이가 없었으나, HB 지표는 4와 5 cm의 횃대를 제공한 처리구에서 일반 평사와 3 cm 두께의 횃대에서 사육한 육계에 비해 개선되었다(p<0.05). 육계가 횃대를 이용한 시간은 사육전기(12~24일령)에는 차이가 없었으나, 사육후기(25~42일령)에 횃대를 이용한 총 시간은 5 cm 두께에서 3과 4 cm 두께보다 높았으며(p<0.05), 올라간 횟수에 비해서도 더 많은 시간을 보냈다(p<0.05). 그러므로 횃대는 육계의 다리질병을 줄이며, 4~5 cm의 두께를 이용하는 것이 가장 적합할 것으로 사료된다.

본 연구는 아파트 단지에 식재된 조경수의 시계열 변화를 파악하고 조경수 중 소나무의 수요를 예측하기 위하여 수행되었다. 2003년부터 2020년까지 수도권의 아파트 단지에 사용된 수목의 종수는 평균 51종이며 2000년대 중후반에 비하여 2010년대 후반에는 다양한 종류의 수목이 사용되었다. 식재 수량은 평균 149,567주/1단지이며 시계열 변화가 두드러지지 않는다. 상록수와 낙엽수의 수종 비율은 2:8 이지만 식재된 수량은 3.5:6.5의 비율이었다. 상록수는 낙엽수에 비하여 한 수종의 식재량이 많은 반면에 낙엽수는 상록수에 비하여 상대적으로 다양한 수종이 식재되고 있다. 교목과 관목의 수종 비율은 6:4이지만 식재량은 관목 97.6%로 식재량에 비해 식재된 수목의 종류는 다양하지 않다. 조경수 중에서도 선호도와 이용가치가 높은 소나무의 식재현황을 분석하면 식재 수량은 증가하는 경향이며 특히, 근원직경이 큰 소나무의 식재 비율이 증가하고 있다. 아파트 단지의 소나무 식재수요를 예측하면 식재량은 지속적으로 증가할 것이며 특히 근원직경 40㎝ 이상의 특수목의 수요가 증가할 것이다. 이러한 조경수의 수요변화에 대응하여 다양한 식물소재를 발굴하고 생산할 수 있는 전략이 요구된다. 특히 지속적으로 소나무의 대형화가 예측되므로 시장에서 요구하는 수형과 규격을 갖춘 소나무가 적기에 공급될 수 있도록 생산관리가 이루어져야 할 것이다.

The planting date of corn for silage has been delayed because of spring drought and double cropping system in Korea. This experiment was conducted to evaluate agronomic characteristics, forage production and feed value of corn at April and May in 2019. Experimental design was a split-plot with three replications. Planting dates (12 April and 10 May) were designated to the main plot, and corn hybrids (‘P0928’, ‘P1543’ and ‘P2088’) to the subplot. The silking days of the early planting date (12 April) was 79 days and that of the late planting date (10 May) was 66 days (p<0.0001), however, there were no significant differences among the corn hybrids. Ear height of the late planting date was higher than that of the early planting (p<0.05), while there were no significant differences in plant height of corn. Insect resistance at the early planting was lower than that of late planting (p<0.05), however, lodging resistance was no significant difference at planting date. The rice black streaked virus (RBSDV) infection of early planting was 3.7% and that of late planting was 0.3% (p<0.001). Dry matter (DM) contents of stover, ear and whole plant had significant difference at planting date (p<0.05). And differences in ear percentages were observed among the corn hybrids (p<0.01). And ear percentages of early maturing corn (‘P0928’) was higher than for other hybrids. Ear percentage at the early planting date was higher than that at the late planting date (p<0.01). DM and total digestible nutrients (TDN) yields had significant difference at planting date, however, there were no significant differences among the corn hybrids. DM and TDN yields at the late planting (21,678 kg/ha and 14,878 kg/ha) were higher than those of the early planting (13,732 kg/ha and 9,830 kg/ha). Crude protein content at the early planting date was higher than that of the late planting. Acid detergent fiber content of the late planting was lower than that of the early planting date (p<0.01), while there were no significant neutral detergent fiber content difference among the corn tested. Calculated net energy for lactation (NEL) and TDN at the early planting were higher than those of at the late planting (p<0.01). Results of this our study indicate that the late planting date (May) is better than early planting date (April) in forage yield and feed value of corn. Therefore, the delay of planting date by May was more suitable for use in cropping system.

To investigate the domestic seed production potential of Italian ryegrass, it was sown in autumn in the southern region and harvested in the spring of the following year to investigate the productivity and quality of seeds and straw. Italian ryegrass (Lolium multiflorum Lam.) ‘GreenCall’ variety was sown in Jinju, Gyeongnam in the fall of 2020 with three seeding rates (20, 30 and 40 kg/ha). The experiment was arranged consisted of a randomized block design with three replications. The ryegrass was sown on October 17, 2020, and the harvest was on May 31, about 60 days from the heading stage. The heading stage of Italian ryegrass was April 28, and there was no difference among treatments. Plant height was significantly shorter in the 40 kg/ha seeding treatment group, and there was no significant difference in the remaining treatments. The resistance of lodging, disease, and cold did not show significant differences among treatments. Spike length and number of seeds per spike were highest at 20 kg/ha seeding amount, and there was no difference in the remaining treatments. The seed yield was the highest at 1,956 kg/ha in the 20 kg/ha seeding rate, and there was no difference in the 30 and 40 kg/ha seeding rates. The dry matter content of seeds and straws was 45.60 and 41.83% on average, and there was no significant difference among treatments. The amount of remaining straw after seed harvesting was found to be 7,689 kg/ha on average on a dry basis, and it was high in the 40 kg/ha sowing area, but there was no significant difference among treatments. According to the above results, it was found that it is most advantageous to sow at 20 kg/ha when producing Italian ryegrass seeds through autumn sowing in the southern region.

This study was carried out to evaluate the growth characteristics and forage yield potential for warm season grass as emergency forages. The experimental design was a randomized block design (RBD) with three replications. Two barnyard millet (Echinochloa species cv. Shirohie and Jeju native), a pearl millet (Pennisetum glaucum cv Feed milk 2) a proso millet (Panicum miliaceum cv Native), a teffgrass (Eragrostis tef cv. Tiffany) and a kleingrass (Panicum coloratum cv. Selection 75) were compared for forage production and quality at the Mid regions of Korea. Warm season forage crops were sown on May 21 and June 23 respectively, and in 2021, it was sown twice on May 21 and June 21 The number of days to seedling emergence for barnyard millet and teffgrass was observed approximately 10 and 3 days after seeding, respectively. The cultivation period from seeding to harvest was within 60 days for all entry spices except for the late-heading type barnyard millet (within 84 days). As for the dry matter yield by seeding date, the dry matter yield of the late-heading type barnyard millet in May seeding was the highest at 23,872 kg/ha, and the kleingrass was the lowest at 3,888 kg/ha. For the June seeding, the dry matter yield of the late-heading type barnyard millet was 17,032 kg/ha, the highest, and the proso millet, teffgrass and kleingrass showed the lowest at 5,468, 5,442, and 5,197 kg/ha, respectively. The crude protein (CP) content was varied by warm season grass species, but the early-heading type barnyard millet, teffgrass, and kleingrass showed the highest tendency, and the late-heading type barnyard millet showed the lowest at 5.7~5.9%. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) content did not show a significant difference between the seeding in May, but kleingrass in June sowed lower than the others.

Somatic cell nuclear transfer (SCNT) in pigs has been used as a very important tool to produce transgenic for the pharmaceutical protein, xenotransplantation, and disease model and basic research of cloned animals. However, the production efficiency of SCNT embryos is very low in pigs and miniature pigs. The type of donor cell is an important factor influencing the production efficiency of these cloned pigs. Here, we investigated the developmental efficiency of SCNT embryos to blastocysts and full term development using fetal fibroblasts (FF) and mesenchymal stem cells (MSCs) to identify a suitable cell type as donor cell. We isolated each MSCs and FF from the femoral region and fetus. Cultured donor cell was injected into matured embryos for cloning. After that, we transferred cloned embryos into surrogate mothers. In term of in vitro development, the SCNT embryos that used MSCs had significantly higher in cleavage rates than those of FF (81.5% vs. 72%) (p<0.05), but the blastocyst formation rates and apoptotic cell ratio was similar (15.1%, 6.18% vs. 20.8%, 9.32%). After embryo transferred to surrogates, nine and nineteen clone piglets were obtained from the MSCs and FF group, respectively, without significant differences in pregnancy and birth rate (50%, 40% vs. 52.3%, 45.4%) (p>0.05). Moreover, there was no significant difference in the corpus hemorrhagicum numbers of ovary, according to pregnancy, abortion, and delivery of surrogate mothers between MSCs and FF groups. Therefore, the MSCs and FF are useful donor cells for production of clone piglets through SCNT, and can be used as important basic data for improving the efficiency of production of transgenic clone pigs in the future.

Laos People’s Democratic Republic (Laos) is a country selected by Korea’s priority foreign policy – New Southern Policy. It is also one of Korea’s 24 priority partner countries. The objective of this study was to understand the current status of rice industry and recommendations for improving rice productivity in Laos based on “Agriculture Development Strategy to 2025 and Vision to the Year 2030” published by the Lao government. In Laos, major crops are rice, vegetables, maize, coffee, cassava, sugar cane, and others. Laos achieved rice self-sufficiency in 1999. Its rice production reached up to 3,580,000 tons in 2018. It aims to harvest a total of 5 million tons of paddy rice with a target yield of approx. 1.5 million tons in 2025. Rice consumption per capita totals 160 kg/year in Laos, the highest in the world. The national nutrient program sets its targets as follows: of 2,600 kcal, a recommended daily calorie intake for adults, it aims to lower rice and starch, while increasing calories from meat, vegetables, fat, and so on. This shows that the rice policy is formulated with consideration of improving health of people in Laos as well as boosting rice exports. Based on the agricultural policy and strategy of Laos, it is viewed that the rice industry in Laos can advance further and produce results only when rice industry is linked to the following areas: economic development, farm size expansion, higher productivity through novel varieties and cultivation technologies, scaling-up of agricultural mechanization, greater efficiency in processing, and infrastructure expansions such as roads and reservoirs. If an ODA (Official Development Assistance) project takes a tailored approach considering its actor-specific characters and tasks, it is believed that the project can contribute to the goal of securing sustainability for the rice industry.

This paper aims to propose a new systematic approach to analyze the factor productivity and to investigate those characteristics of factor productivity in operational and managerial perspectives. The Cobb-Douglas production function is adopted to estimate the labor and capital productivity. In estimating those productivities the data of The Research on the Actual Condition of Coastal Fisheries (RACF), especially those of Jeon-Nam Province are used. The statistical analysis of RACF data shows that the characteristics are a little bit different between labor and capital of the operational equipment in the coastal fisheries. The Cobb-Douglas type production function is useful in estimating the factor productivity, especially in case of ‘coastal Stow-net fishery’ even though the limited data is used. However, in case of ‘trap fishery,’ the Cobb-Douglas production function appears to have some limitations in estimation. This implies that estimating the factor productivities in fisheries employing broad perspectives and various methods are needed.

This study was conducted at the National Institute of Animal Science (NIAS) from 2010 to 2017 to develop a late-maturing variety with high productivity for cultivation in the southern region of South Korea. The new variety of Italian ryegrass, ‘IR901’, was a late-maturing variety, and its heading date was 22 May, 17 days later than that of the control variety ‘Kowinearly’. ‘IR901’ had a flag leaf width of 11.2 mm, flag leaf length of 31.8 cm, and plant length of 103 cm on its heading date. The combined average dry matter yield of ‘IR901’ in all three adaptability evaluation regions (Cheonan, Pyeongchang and Haenam) was 7,747 kg/ha, which was similar to that of the ‘Kowinearly’ variety (7,734 kg/ ha). However, the average dry matter yield over three years in Cheonan and Pyeongchang was 82% and 96%, respectively, compared to that of the control, which was most likely because of the poor cold tolerance of ‘IR901’. By contrast, in Haenam, in the southern region, the average dry matter yield of ‘IR901’ was 19% higher than that of the ‘Kowinearly’ variety. The proportions of crude protein (CP), total digestible nutrients (TDN), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in ‘IR901’ were 8.6%, 59.7%, 36.9%, and 54.8%, respectively; the proportions were 0.2% lower, the same, the same, and 2.5% lower than those in the ‘Kowinearly’ variety. The determined in vitro dry matter digestibility (IVDMD) of ‘IR901’ was 72.2% higher than that of ‘Kowinearly’ (67.2). In general, of the two varieties, the forage quality of ‘IR901’ was marginally superior to that of ‘Kowinearly’.

동물 화분매개는 대부분 벌과 파리 등의 곤충에 의해서 이루어지는 매우 중요한 생태계 서비스 중 하나이다. 농업생산성의 유지와 영양분의 안정적 공급은 인류 복지의 근간이 된다. 이 연구는 한국 농업에서 화분매개가 식량 생산 증진에 미치는 기능을 평가하였다. 2015년 농업총조사, 농촌진흥청 농업 소득자료집, 기타 시장가격 자료 등의 농작물 재배와 생산액에 대한 자료에 화분매개 의존도를 반영하였다. 12개의 식량작물, 19개 과수, 18개 노지 채소와 13개 시설 채소 그리고 9개의 특용작물 등 71개 작물을 대상으로 한 분석에서, 전체 작물의 화분매개 의존도는 평균 29.2%이었으며, 식량작물이 가장 낮은 7.5%로 나타났다. 화분매개 의존량은 전체 농작물의 17.8%이었으며 그 경제적 가치는 6조 8.5천억원 (65.1-71.9천억원)으로 추정되었다. 화분매개 의존 생산량은 시설 과채류에서 49.2%로 가장 높았고 과수가 42%, 특용작물이 35.9%를 차지하 였다. 본 연구를 통해 곤충 화분매개는 한국 농업 생산에서 핵심적 과정이며 식량-영양안보적 관점에서 보호증진해야 하는 과정임이 분명해졌다. 농업에서 화분매개자를 보호하고 화분매개서비스를 강화할 수 있는 관리 전략으로 병해충-화분매개 통합관리(IPPM)개념과 추진 전략에 대하여 추가적으로 논의하였다.

체외 환경에서 생산되는 배아 (Embryo)는 활성산소종 (Reaction oxygen species, ROS) 수준이 일정 수준을 초과함에 따라 산화적인 손상을 받게 된다. 선행연구에 따르면, 항산화제는 ROS를 감소시켜주는 효과를 가지기 때문에 ROS로부터 오는 배아의 단백질, DNA의 손상, 세포 자멸사를 방지하여 배아의 발달률을 향상시킨다. 이전연구에 따르면 항산화제로써 엘라그산 (Ellagic acid, EA)은 ROS를 효과적으로 제거하고, 난자의 산화스트레스를 방지하는 효과를 가지고 있다고 보고되었다. 그리하여, 본 연구를 통해 우리는 소의 수정란 배양체계 중 in vitro culture (IVC) 단계에서 EA의 농도 (0, 5, 10 μM) 별 첨가가 소의 수정란 발달률과, 질적 수준에 미치는 영향을 조사하고자 실험을 진행하였다. 결과적으로, 배반포의 단계별 발달 수준에서 cleavage 형성률은 EA첨가군과 대조군 간의 차이를 발견할 수 없었으나 배반포 형성률에서는 모든 EA 첨가군들이 대조군보다 높았고 EA 첨가군 중에 5 μM 첨가군이 가장 높았다 (p < 0.05). 생산된 배반포의 총 세포 수는 5 μM EA 첨가군이 대조군과 10 μM EA 첨가군 보다 유의적으로 높았으며, 대조군과 10 μM EA 첨가군 사이의 유의적 차이는 없었다 (Control vs. 5 μM vs. 10 μM; 137 ± 7.90 vs. 163.2 ± 7.42 vs. 138.8 ± 6.67, p < 0.05). 세포 자멸사 세포 수는 모든 EA 첨가군들이 대조군보다 유의적으로 낮았다 (Control vs. 5 μM vs. 10 μM; 22.65 ± 4.08, 9.61 ± 1.55, 6.14 ± 0.90, p < 0.05). ROS 수준에서 모든 EA 첨가군들과 대조군 간의 유의적 차이는 없었다 (Control vs. 5 μM vs. 10 μM; 6.81 ± 1.31, 3.86 ± 0.23, 4.11 ± 0.18, p < 0.05). qRT-PCR 실험 결과에서 Nrf2 gene expression은 대조군과, 5 μM 첨가군에서 유의적 차이가 없었으나, 10 μM 첨가군에서는 유의적으로 상향 조절된 것을 관찰하였다. Keap1 gene expression은 5 μM 첨가군에서 유의적으로 하향 조절된 것을 관찰하였다. 하지만 EA의 농도가 10 μM으로 높아짐에 따라 발현 수준이 증가한 것을 관찰할 수 있었다. CAT gene expression은 5 μM 첨가군에서 유의적으로 상향조절 되었으나 10 μM 첨가군에서는 유의적인 차이를 보이지 않았다. SOD1 gene expression은 대조군과 5 μM 첨가군은 유의적인 차이를 보이지 않았으나 10 μM 첨가군에서는 유의적으로 상향 조절된 것을 관찰하였다.

본 고는 국내 최초 극장상영 연쇄극 영화<의리적구투>의 영화사적 가치와 문화적 가치를 반영하여 당시의 영화를 현대적 감각으로 재현하기 위해 활용할 무대의 영상을 기획 연구하였다. 연구에서 오늘날 공연’트렌드(trend)‘와 영화문법을 적용하여 본 공연을 위한 무대 영상제작의 방향성을 제시한다. 공연은 현재 합천’영상테마파크’에 조성된 실내 ‘단성사 극장’과 야외 ‘조선총독부, 경성역’을 무대 공간으로 설정하였다. 실내 단성사 극장 무대에서는 영상과 배우연기, 악기연주, 가수, 변사가 당시 공연을 재현했다. 야외공연에는 조선총독부와 경성역 중 한 곳을 선택하여 극 중 주인공 송산의 영화적 현실과 당시 일제 치하 조선인들의 고달픈 삶과 역사적 사건을 일치시킨 ‘미디어파사드’ 방식의 기호화 영상을 예술적으로 제작하기 위해 기획하였다. 오늘날 뮤지컬공연이나 연극 공연에서 활용되는 무대 영상이 단순히 공연내용의 배경이거나 미학적 영상이었다면, 본 공연의 무대 영상은 영화문법을 반영한 영상으로 한 편의 영화를 공연화 하는 작업이라고 할 수 있다. 다시 말해서 영화적인 영상과 무대 위에서 연기하는 배우가 하나가 되는 공연형식의 영화 영상이라고 말할 수 있다.

난자의 성숙과정과 노화에 관한 이해는 인공수정과 체외수정 최적기를 판단하기 위하여 가장 중요한 연구내용으로 알려져 있다. 이러한 기작은 번식 호르몬들에 의하여 조절되는 것으로 알려져 있으나 난자 세포질 변화에 관한 내용은 잘 알려져 있지 않다. 본 연구에서는 산화질소물(nitric oxide, NO)이 난자 성숙과정에서 증가하는 것을 밝혔으며 난자의 미성숙단계(germinal vesicle stage, GV)와 난자핵막붕괴단계(germinal vesicle breakdown, GVBD) 및 성숙완료단계(metaphase II, MII)단계에서 생산되는 NO의 양을 비교하였다. 또한, 난자를 체외에서 배양할 때, MII단계로 성숙되지 않는 성장 단계의 난자에서는 NO의 증가 현상을 관찰할 수 없었고, 세포질이 불균일한 노화된 난자에서는 NO가 증가된 상태로 유지되는 특성이 있음을 밝혔다. 이러한 결과는 NO의 작용이 난자의 성숙과정과 난자 노화과정에서 중요한 기능을 담당하고 있음을 보여주고 있다.

Uniform scions and rootstocks should be produced to ensure grafting success. Light quality is an important environmental factor that regulates seedling growth. The effects of warm- and cool-white light emitting diode (LED) ratios on seedling growth were investigated. Scions and rootstocks of cucumber, tomato, and watermelon were grown in a closed transplant production system using LED as the sole lighting source. The LED treatments were W1C0 (only warm-white), W1C1 (warm-white: cool-white = 1:1), W3C1 (warm-white: cool-white = 3:1), and W5C2 (warm-white: cool-white = 5:2). The seedlings grown in W1C1 had the shortest hypocotyls, and the seedlings grown in W1C0 had the longest hypocotyls among the three tested vegetables. The hypocotyls of watermelon scions, watermelon rootstocks, and tomato rootstocks were shortest in W1C1, followed by those in W3C1, W5C2, and W1C0, but there was no significant difference between W3C1 and W5C2, which remained the same as the ratio of cool-white LEDs increased. In addition, tomato scions had the first and second longest hypocotyls in W1C0 and W3C1, respectively, and the shortest hypocotyls in W5C2 and W1C1, along with W5C2 and W1C1, although the difference was not significant. The stem diameter was highest in W1C0 except for tomato seedlings and rootstocks of watermelon. The shoot fresh weight of scions and rootstocks of cucumber and watermelon and the root fresh weight of cucumber scions were lowest in W1C1. These results indicated that different ratios of LED lighting sources had a strong effect on the hypocotyl elongation of seedlings.