The present study was designed to determine the effect of barn or cycle of grazing on changes of biochemical metabolites in prepartum and changes of milk composition in postpartum of dairy cows. For this purpose, a total of sixteen 25 months old Holstein primiparous dairy cows were allocated in two groups (n=8) with an average body weight of 571.61 ± 35.30 kg (Barn) and 578.10 ± 39.20 kg (Grazing). The study was conducted from June 2018 to October 2018. Results revealed that barn raised dairy cows had a higher increase in their serum albumin and calcium level on day 14 prepartum. However, the level of palmitic acid, saturated fatty acid increased significantly, and the level of fat, oleic acid, γ-linoleic acid, arachidonic acid and unsaturated fatty acids decreased significantly in barn raised dairy cow’s milk on day 14 postpartum. There were no significant differences observed with respect to all other biochemical metabolites, fatty acids and minerals between barn raised and cycle grazing dairy cows during prepartum and postpartum. Our study results could serve to a better understanding of barn raised cow with respect to changes of biochemical metabolites in prepartum and changes of milk composition, fatty acids and minerals content in grazing dairy cows in postpartum for estimating their physiological status.

This study was conducted to investigate the effects by supplementing concentrates during the late fattening phase in TMR (total mixed ration) feeding of Korean steer on the performance, carcass traits, physico-chemical characteristics and sensory test of the longissimus dorsi muscle. Eight Korean steers (24 months of age) averaging 604 kg in body weight were fed the TMR (Control) or the TMR supplemented with concentrates (TMR+conc.) until 30 months of age, then they were slaughtered. Steers fed the TMR supplemented with concentrates had a higher (p<0.05) intakes of dry matter and TDN (total digestible nutrient), carcass weight and marbling score compared to those fed TMR only, but carcass grade did not differ (p>0.05) between treatments. Amino acid concentration of the longissimus dorsi muscle did not differ between treatments, However, the highest concentrations were for glutamic acid and lowest for cystine; further, and the content of essential amino acids was highest for lysine, leucine, threonine, arginine, and isoleucine in that order. Cis-oleic acid and arachidonic acid of fatty acids in the longissimus dorsi muscle were higher (p<0.05) in the control condition compared to TMR+conc. The contents of oleic acids, palmitic acid, stearic acid constituted about 88% of the total fatty acids. Although the physico-chemical characteristics of the longissimus dorsi muscle did not significantly differ between treatments (p>0.05), but the sensory test results were lower for the TMR+conc. condition. These results suggest that supplementing concentrates during the late fattening phase of Korean steers resulted in increasing the carcass weight and marbling score. However, the cis-oleic acid content of the longissimus dorsi muscle was decreased as a result of supplementing concentrates, which could affect negatively in meat sensory evaluation.

본 연구는 국내의 고온다습한 하절기에 산란계에 음용수 온도가 생산성, 호르몬 농도 및 혈액성상에 미치는 영향을 구명하고자 실행하였다. 52주령 하이라인 갈색종 산란계 432수를 3개 처리구와 8반복으로 반복당 18수씩 케이지 사육장(550 cm2/수)에 배치하였고, 처리구별로 14.5, 24.0 및 32.5℃의 음용 수를 4주간 급수하였다. 산란계의 생산성은 산란율과 난중을 매일 조사하여 계산하였고, 호르몬, 혈액 성상 및 계란품질은 사양실험 종료 후 채집하여 분석하였다. 본 연구결과 산란율과 1일 산란양은 14.5℃ 의 급수구에서 유의적으로 개선되었고(p<0.05), 사료섭취량과 난중은 14.5와 24.0℃ 급수구에서 32.5℃ 에 비하여 현저히 증가하였다(p<0.05). 계란의 난백높이와 호우유닛은 음용수 온도에 따른 처리구간 통계적 차이가 없었다. 난각강도는 14.5℃ 급수 처리구에서 증가하였고(p<0.05) 난각두께도 개선되는 경향을 보였지만 처리구간에 통계적 차이는 없었다. 또한 혈청과 간의 GH와 IGF-I 농도는 32.5, 24.0 및 14.5℃ 급여구 순서로 증가하였고(p<0.05), 코티코스테론은 감소되었는데 14.5℃에서 가장 개선되었다(p<0.05). 이외에도 혈중 AST와 콜레스테롤은 14.5℃의 음용수 처리구에서 24.0과 32.5℃에 비하여 유의적으로 감소하였고(p<0.05), HDL콜레스테롤, 단백질 및 글루코오스는 처리구간에 통계적 차이가 없 었지만, 혈중 중성지방은 14.5℃처리구에서 현저하게 감소되었다(p<0.05). 그러므로 여름철 14.5℃의 음용수를 급여하면 산란계에서 고온스트레스를 저감하므로서 생산성, 계란품질 및 혈액성상을 개선하였다.

본 연구는 고온다습한 하절기에 음수의 형태가 닭의 생산성, 육질, 혈액의 성상에 미치는 영향을 구명하고자 실행하였다. 8일령 로스종 360수를 4개 처리구인 수돗물, 이온수, 냉수 및 냉이온수(냉수+이온수)로 처리구당 6반복으로 평사에 수용하여 5주령까지 급수하였다. 계사내부 평균 온도와 습도는 30.9℃와 74.0%였으며, 수돗물의 평균온도는 29.5℃였다. 이온수는 정전기를 이용하여 물을 이온화하였고, 냉수는 15℃로 고정하여 급수하였다. 증체량, 사료섭취량 및 사료요구율은 주간으로 측정하였으며, 혈액과 계육 샘플은 실험 종료시에 채취하였다. 사육전기 21일령에 증체량은 수돗물 처리구에서 다른 처리구에 비하여 낮았지만 통계적 차이는 없었으며, 사육후기의 35일령에 이온수, 냉수, 냉이온수 처리구에서수돗물 처리구에 비하여 현저하게 높게 나타났다(p<0.01). 사료섭취량은 증체와 동일하게 사육전기에는 처리구간에 일관적 차이가 없었지만 사육후기에 수돗물 처리구에 비하여 다른 처리구에서 매우 개선 되는 경향을 보였다(p<0.01). 사료요구율은 사양실험 전기간에서 이온수, 냉수 및 냉이온수 처리구에서 매우 개선되었다(p<0.05). 계육의 가슴살에서 명도는 수돗물 처리구에서 높게 나타났으며, 적색도는 낮은 경향을 보였다. 황색도는 일관성이 없었지만 pH는 수돗물 처리구에서 다른 처리구에 비하여 낮게 나타났다(p<0.05). 조리감량은 이온수, 냉수 및 냉이온수 처리구에서 수돗물에 비하여 매우 낮았지만(p<0.05) 연도는 높은 경향을 보였다. 혈중 알부민과 단백질은 처리구간에 차이가 없었지만, 중성지방은 이온수, 냉수 및 냉이온수 처리구에서 낮은 경향을 보였다(p<0.05). 혈중 포도당은 처리구간에 차이가 없었지만 HDL 콜레스테롤은 이온수, 냉수, 냉이온수 처리구에서 수돗물 처리구에 비해 현저하게 높게 나타났다(p<0.05). 본 실험 결과 혹서기 육계에 냉이온수 급수는 생산성 및 육질을 개선하고 혈중HDL을 높게 하였다.

The objective of this study was to investigate the effects of freeze dried placenta supplementation on reproductive performance, colostrum and plasma biochemical composition in pregnant sows. Eleven Landrace × Large white sows were fed with corn-soybean meal diets supplemented with or without 1% freeze dried placenta powder from 10 days before their expected farrowing dates until 10 days postpartum. The colostrum protein content was significantly higher(P=0.043) in the treatment group than in the control group. Compared to the control group, the immunoglobulin G(IgG) concentration in the colostrum was significantly higher(P=0.004) in the treatment. In day 25 piglets plasma, the IgG concentration was higher(P=0.184) in the treatment than the control. The mortality rate was lower(P=0.102), and the piglet weight gain was higher(P=0.35) in the treated group. Overall, the treatment group showed greater levels of protein and IgG concentration in the colostrum, when compared to control group. Therefore, the freeze dried placenta supplementation on pregnant sows can enhance its colostrum composition, hence decrease the mortality and increase the growth rate of piglets.

The marine microalgae Isochrysis galbana was cultured under various light–emitting diode (LED) light conditions with different wavelengths to examine changes in growth and in amino acid and fatty acid profiles. The culture conditions for the microalgae were Conway medium, salinity of 33 psu, temperature of 24°C, and a 16/8 h light/dark photoperiod. Six light sources, including 5 units of 180W LED lamps (peak wavelength: blue [LB] 470 nm; green [LG] 525 nm; yellow [LY] 595 nm; red [LR] 636 nm; white [LW] 442 nm) and 1 unit of a 175W metal halide (MH) lamp, were used for the experiment. The dry cell weights (gL–1) of I. galbana under different light conditions were in the order of LW>LB≥MH>LR>LG>LY. Levels of essential amino acids were revealed to be significantly higher under LW, LG, and MH than under the other wavelengths (P<0.05). The fatty acid, unsaturated fatty acid, and DHA contents of I. galbana were higher under MH, LW, and LG. In addition, the carotenoid content was higher under MH, LW, and LG than under the other wavelengths (P<0.05). The fucoxanthin content was highest under MH (0.28%) and lowest under LY (0.2%), and it was 0.26% under LW and LG. The results indicate that the combined use of LW and LG is effective when using LED lamps for I. galbana cultivation.

The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR≥ CON>LB≥LW≥LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures (6.3µg/mL), whereas LW showed the lowest (3.6µg/mL; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

연안생태계의 영양상태와 환경적 질을 평가하기 위하여, 2007년 2월 남해연안의 총 25개 소해역, 131개 정점을 대상으로 표층 퇴적물의 입도조성, 광합성 색소, 총유기탄소, 총질소, 생화학적 조성(단백질, 탄수화물, 지질) 등을 분석하였다. 다차원 분석 결과 4개의 그룹으로 나뉘었으며, 일원분산분석에서 단백질, 탄수화물, biopolymeric carbon 농도의 차이가 뚜렷하게 나타났다. 이를 토대로 퇴적물 내 생화학적 조성을 이용한 영양상태 기준을 설정하였으며, 조사해역의 영양상태를 평가한 결과 산업폐수 및 생활하수 유입이 많은 마산만, 진해만, 행암만을 포함한 I그룹을 과영양 상태, 양식시설이 밀집한 통영, 고성 자란, 거제연안 등의 II그룹은 부영양 상태로 추정되었으며, 가막만, 득량만, 여자만 등의 III그룹은 중영양 상태로, 나머지 신안, 진도, 무안을 포함한 IV그룹은 빈영양 상태로 추정되었다. 본 연구결과로 퇴적물의 생화학적 조성을 이용한 영양상태 구분은 연안역의 영양도 평가를 위하여 유용한 방법으로 사용되어 질 수 있다고 판단된다.

The purpose of this study was to investigate the body composition, biochemical parameters, and consumption of convenience foods according to β-3 adrenergic receptor polymorphism in university students. A survey was conducted on a total of 486 students - 189 males and 297 females. Based on a self-reporting method, questionnaires were administered for over 20 minutes, and β-3 adrenergic receptor and blood samples were also analyzed. The genotype frequencies of β-3 adrenergic receptor polymorphism were Trp/Trp homozygote (73.0%) and Trp/Arg heterozygote (27.0%) in male students. For the female students, the distribution of genotypes was Trp/Trp (71.0%) and Trp/Arg (29.0%). There were no differences according to biochemical parameters (ALT, cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, and hemoglobin) or body composition. Males with TT genotype frequently ate Ramyon (2.40±0.52), Cup Ramyon (2.37±0.39), Kimchi (2.23±0.61), and frozen meat (2.00±0.44), whereas males with TA genotype ate Fries (frozen food) (1.90±0.79), Smoked meat (1.67±0.81), and Canned fruit (1.64±0.81). Females with TT genotype frequently ate Frozen fries (2.21±0.35), Kimbab (2.12±0.44), and Ramyon (1.85±0.40), whereas females with TA genotype frequently ate Kimchi (1.73±0.98), Fries (frozen food) (1.46±0.26), and Cup Ramyon (1.30±0.34). When questioned about satisfaction about body shape, 22.8 and 60.8% of those with TT genotype answered that they were 'satisfied' or needed to 'lose weight', respectively, whereas 18.0 and 63.9% of those with TA genotype answered that they were 'satisfied' or needed to 'lose weight', respectively. In conclusion, this study found no significant effects in terms of β-3 adrenergic receptor polymorphism, which suggests that health-promoting education needs to be developed so that university students appropriately recognize their bodies and control their weight in desirable ways. Therefore, it is necessary to educate individuals with TT genotype how to buy reasonable foods by understanding the interrelationship between convenience foods and health care and by checking the nutrition index labels on convenience foods. Thus, it is recommended that a health-promoting program be developed for the promotion of healthy lifestyles.

The purpose of this study was to investigate the effects that body composition, nutrient intake, and biochemical indices have on skin status, as well as their associations with skin health status, by assessing 44 female university students. The subjects were classified into 3 groups according to skin sensitivity: sensitive skin (n=11), slightly sensitive skin (n=24), and normal skin (n=9). The study included anthropometric measurements and body composition analyses, dietary intake evaluations, blood chemical analyses, and skin health status assessments. Based on % body fat, the results indicated the subjects were moderately obese (29.6%). Obesity indices were not different among the groups, but the sensitive skin group tended to have higher skinfold thickness. Dry skin was determined as the skin type of all subjects. In the sensitive skin groups, U-zone and T-zone sebum were significantly lower (p〈0.05), and spots were significantly higher (p〈0.05) than in the normal skin group. In terms of nutrient intakes, the sensitive skin groups consumed significantly lower amounts of P and vitamins A and E (p〈0.05). There were no significant differences in serum lipid fractions or total antioxidant status among the groups. In correlation coefficients analysis, skin elasticity had significant negative correlations with subscapular and midaxillary skinfold thickness, body protein, and body minerals (p〈0.05). Wrinkling had positive correlations with triceps and thigh skinfold thickness (p〈0.05). Skin evenness had negative correlations with energy intake, vitamins A, B1, B2, C, E, niacin, and zinc (p〈0.05), as well as folate and P (p〈0.01) and vitamin B6 (p〈0.001). A similar correlation tendency was observed for spots and nutrient intake, showing negative correlations with vitamins A, B6, and E, as well as folate, Fe, and P. U-zone sebum showed significant positive correlations with serum levels of total cholesterol and triglycerides. From these findings, skin health status appears to be affected by both nutrient intake and body composition. Therefore, having a proper balance between dietary intake and body composition may influence skin health status in females with sensitive skin.

질산염과 인산염의 농도가 다른 배지에서 Gymnodinium sanguineum과 Sketetonema costotum의 성장과 세포구성성분을 비교 분석하였다. 질산염은 0, 0.3, 0.6, 0.9, 1.2 mM, 인산염은 0, 15, 30, 45, 60μM을 각각 첨가한 배지에서 배양하였을 때, 저농도의 질산염 및 인산염 배지에서 G. sanguineum의 성장은 억제되었으나, S. costatum은 성장에 영향을 받지 않은 것으로 나타났다.