The objective of this study was to determine the ultrasonication-assisted extraction conditions that maximize the DPPH radical scavenging activity of extracts obtained from the stems of Lespedeza bicolor Turcz through the application of the Response Surface Methodology (RSM). Before delving into the analysis of extraction conditions using the RSM model, we conducted efficiency validation of ultrasonication-assisted extraction and executed single-factor experiments for ethanol concentration, extraction time, and extraction temperature. The data obtained from these single-factor experiments were employed to construct the Box-Behnken Design (BBD). In these results, in the single-factor experiments, it was evident that the parameters for ethanol concentration, extraction time, and extraction temperature exhibited quadratic trends. The single-factor experiments allowed us to discern the trends for each parameter leading to the maximum antioxidant capacity, and this data was subsequently applied to the BBD. Following the completion of initial experiments, a Response Surface Methodology (RSM) model was constructed based on Box-Behnken Design (BBD). According to the predictive model developed in this study, it was anticipated that performing ultrasonic-assisted extraction for 85.0412 minutes at an ethanol concentration of 32.573% and an extraction temperature of 51.5608°C will result in a DPPH radical scavenging activity of 79.7146%. The predictive results were statistically verified through a comparative analysis with actual measurements and ANOVA analysis, confirming the statistical significance of the model. The finding of this study underscore the significance of optimizing extraction conditions in the precise quantification of the antioxidant potential for economic advantages in both experimental and industrial contexts.

This study is concerned with the optimization of the manufacturing process of a hot water extract containing antioxidant activity from Lycium barbarum, traditionally known to have various physiological activities. For the establishment of the optimization process, the central composite design of response surface methodology(RSM) was used. Thirteen extraction processes were performed by encoding the independent variables, extraction temperature (65.9oC–94.1oC) and extraction time (2.59 hr–5.41 hr). As a result of the experiment, the optimal manufacturing conditions for the extract were 340.0 mg/100 g of GAE at an extraction temperature of 94.1oC and an extraction time of 5 hr. The maximum yield of flavonoids was 22.44 mg/100 g of HES at an extraction temperature of 94.1oC and an extraction time of 4 hr. The conditions for producing the extract with the maximum antioxidant capacity (DPPH 92.12%) were 90oC and 4.5 hr extraction time. Therefore, the optimal manufacturing process conditions for extracts containing total phenol content, flavonoid content, and DPPH radical scavenging activity, which are dependent variables, were extraction temperature of 90-95oC and extraction time of 4 hr, which were not significantly different from the actual values. Therefore, Lycium barbarum extract rich in total phenol and flavonoid content related to antioxidant function is expected to be used as a functional food and cosmetic material.

Background: The serratus anterior is one of the most important muscle for maintaining good scapular alignment in the shoulder joint. The pectoralis major and upper trapezius may also compensate for weak serratus anterior muscles. The push-up plus exercise has been identified as the optimal exercise for maximum activation of the serratus anterior. Objects: The purpose of this study was to examine differences in surface electromyography (EMG) activity of upper trapezius, pectoralis major, and serratus anterior muscles during push-up plus exercises on variously angled surfaces in subjects with winged scapula. Methods: Sixteen subjects with winged scapula (male=5, female=11) volunteered for this study. The subjects performed push-up plus exercise on four different tilt angles, namely 0˚, 30˚, 60˚, and 90˚. EMG activities in the serratus anterior, upper trapezius, and pectoralis major muscles during performance of push-up plus exercise were measured in all subjects. Data were processed from repeated measures one-way analysis of variance. Results: There was significant difference in the muscle activity of the serratus anterior on the different surface angles (p＜.05). The results of the post-hoc analysis showed significantly greater serratus anterior muscle activity on a surface at a 0˚ angle than at others tilt angles (p＜.05). There was also significant difference in the ratio of serratus anterior to upper trapezius and serratus anterior to pectoralis major across the four surfaces (p＜.05), and post-hoc analysis showed significantly greater values on the 0˚ surface than on other tilts (p＜.05). Conclusion: This study found that performing push-up plus exercises on a flat surface with 0˚ and 30˚ tilt angle achieves high activation of the serratus anterior muscle for selective strengthening. It can also take into account the sequential application, which is first performed at a 30˚ and at a 0˚ tilt angle for and effective but not excessive muscle activation.

목적 : 본 연구는 저항운동으로 6주간 적용된 혀-유지 메뉴버 프로그램이 Shaker 운동 프로그램과 비교하여 정상성인의 삼킴 근육 활성도에 미치는 영향을 알아보고자 하였다. 연구방법 : 2015년 10월부터 12월까지 10대에서 50대의 건강한 성인 26명을 대상으로 혀-유지 메뉴버 그룹과 Shaker 운동 그룹으로 무작위 할당하여 주 5일, 하루 1회, 30분씩 6주간 적용하였다. 표면근전도를 사용하여 설골상근 (suprahyoid muscle)과 설골하근(infrahyoid muscle)에 대한 근활성도를 수집하였다. 시각적 비율척도(Visual Analog Scale), 자각인지 척도(Borg-Rating of Perceived Exertion Scale) 척도를 사용하여 삼킴 시 노력과 피로를 측정하였다. 결과 : 혀-유지 메뉴버 그룹과 Shaker 그룹 모두 설골상근과 설골하근의 근활성도가 유의한 향상을 보였지만, 두 그룹 간의 유의한 차이는 없었다. 또한 두 그룹 모두 삼킴 시 노력 및 피로가 통계학적으로 유의한 차이를 보였으나, 두 그룹 간의 유의한 차이는 없었다. 결론 : 건강한 성인을 대상으로 6주간 혀-유지 메뉴버를 통해 삼킴 근육 근활성도에 대한 긍정적인 효과를 확인하였다. 향후에는 삼킴장애 환자를 대상으로 혀-유지 메뉴버에 대한 임상적 효용성을 검증하여 유용한 치료기법으로 사용되기를 기대한다.

The purpose of this study was to determine the optimum conditions of solvent extraction type and solvent concentration (60, 70, 80%, v/v), extraction time (30, 80, 130 mins) and extraction temperature (10, 15, 20℃) in order to increase the antioxidant activity of the chia seed. The total polyphenol content and DPPH radical scavenging activity was measured by using response surface methodology (RSM) to establish the optimal conditions. Using ethanol and methanol extractions at extraction concentrations of 60%, and time and temperature of 130 mins and 20℃, the maximum total polyphenol content was 871.00 mg% (R2=0.9507) and 557.70 mg% (R2=0.9784) for ethanol and methanol extraction respectively. Using the same extraction conditions, the maximum level of DPPH radical scavenging activity was 72.14% (R2=0.9675) and 52.79% (R2=0.9524) for ethanol and methanol extraction respectively. The results indicate that ethanol extracts showed a higher antioxidant activity than methanol extracts. The ethanol extraction conditions of response surface analysis (RSA) were affected more by ethanol concentration than by extraction time or temperature. In contrast, the methanol extraction conditions of response surface analysis (RSA) were affected more by extraction time. Based on the RSM, the optimum ethanol extraction conditions were the following: extraction concentration, 63%: time, 100 mins: and temperature, 18℃. The optimum methanol extraction conditions were the following: extraction concentration, 65%; time, 120 mins; and temperature, 16℃.

To synthesize a high-performance photocatalyst, N doped TiO2 nanotubes deposited with Ag nanoparticles were synthesized, and surface characteristics, electrochemical behaviors, and photocatalytic activity were investigated. The TiO2 nanotubular photocatalyst was fabricated by anodization; the Ag nanoparticles on the TiO2 nanotubes were synthesized by a reduction reaction in AgNO3 solution under UV irradiation. The XPS results of the N doped TiO2 nanotubes showed that the incorporated nitrogen ions were located in interstitial sites of the TiO2 crystal structure. The N doped titania nanotubes exhibited a high dye degradation rate, which is effectively attributable to the increase of visible light absorption due to interstitial nitrogen ions in the crystalline TiO2 structure. Moreover, the precipitated Ag particles on the titania nanotubes led to a decrease in the rate of electron-hole recombination; the photocurrent of this electrode was higher than that of the pure titania electrode. From electrochemical and dye degradation results, the photocurrent and photocatalytic efficiency were found to have been significantly affected by N doping and the deposition of Ag particles.

백두산에서는 2002년부터 화산성 지진활동의 증가, 정밀 수준측량 관측을 통한 지표면 팽창, 특정 화산가스 방출량의 증가, 온천수의 온도 증가 등 화산 전조활동이 활발해짐으로써 나타나는 전조현상들이 관측되었다. 이러한 관측결과 중 정밀 수준측량 데이터를 활용한 백두산 천지 칼데라 주변의 수직, 수평 지표변위 경향 분석을 통해 2002년 이후 2009년까지 지표가 팽창하는 경향을 나타냄으로써 백두산 지하 마그마 챔버의 부피가 증가하였음을 확인할 수 있었다. 이후 2010년을 기점으로 지표가 수축하는 경향을 나타내기도 하였으나 이후 다시 팽창하는 경향을 나타냄을 확인할 수 있었다. 북측 사면에서 2002년부터 2003년까지 칼데라 외륜산 일대의 지표면이 46.33 mm 융기 팽창한 것을 바탕으로 지하 마그마 챔버의 부피 변화량을 계산해 보면, 약 0.008 km3 (7.7-8.0×106m3)의 부피가 증가한 것으로 계산되었다. 이는 백두산 산정부로부터 지하 약 5 km 지점에 천처 마그마 챔버가 형성되고 0.008 km3의 마그마가 주입된 것으로 해석된다.

The purpose of this study was to determine the effect of the changes that occur in the leg muscle activity of unstable surface with different levels of air pressures. Three groups of college students have been placed randomly on unstable surfaces with different air pressures at group 1.0 psi (=36), group 1.4 psi (=40), and group 1.8 psi (=40). Using surface electromyography, the recruitment of the tibialis anterior, peroneus longus, and the gastrocnemius was measured. Maximal voluntary isometric contraction was measured at the different air pressures based on the manual muscle test, then normalizing the value to %maximal voluntary isometric contraction (%MVIC). The tibialis anterior muscle activity was significant change from the unstable surface with difference levels of air pressures between group 1.0 psi and 1.8 psi and between group 1.4 psi and 1.8 psi. peroneus longus muscle activity was significant changes in muscle activity occurred between 1.0 psi and 1.4 psi group and between 1.0 psi and 1.8 psi group. Gastrocnemius muscle activity was significant change in muscle activity occurred between 1.0 psi and 1.4 psi group and between 1.0 psi and 1.8 psi group. In conclusion it identify that 1.0 psi group is most effective on muscle activity than the other groups. These suggest that the rehabilitation or strengthening of patients with ankle injuries, balance exercise with low air pressure like 1.0 psi can be more effective.

개똥쑥 추출물의 항산화 활성 및 그 유효물질의 함량을 최적화할 수 있는 추출 조건을 확립코져 반응표면 분석법에 의한 중심합성계획에 따라 15가지의 추출 조건(온도: 50∼90℃, 시간: 3∼15 hr, 에탄올 농도: 0∼100%)에서 실험하였다. 개똥쑥 추출물의 DPPH, ABTS 라디칼 소거활성 및 환원력은 J 조건(90℃, 9 hr, 50%의 에탄올 농도)에서 가장 높았으며, NO 라디칼 소거활성은 H 조건(80℃, 12 hr, 75%의 에탄올 농도)에서 가장 높았다. 총 페놀 화합물 및 플라보노이드 함량은 J 조건에서 가장 많았으며, 다음으로 H 조건의 추출물이었다. 반응표면분석 결과 개똥쑥의 최적 추출조건은 평균 89.37℃, 8.55 hr 및 56.77% 의 에탄올 농도인 것으로 나타났으며, 실측값은 예상값의 89.88∼115.54%에 해당되었다.

The aim of this study was to determine the muscle activity of the abdominalis and erector spinae during bridging and unilateral bridging exercises on the firm surface, the sir-fit, and the foam roll. Eighteen healthy young subjects were recruited for this study. Surface electromyographic (EMG) activities were recorded from the both sides of the rectus abdominalis, external obliques, internal obliques, and erector spinae muscles during bridging and unilateral bridging-exercises. A one-way repeated analysis of variance was used to compare the EMG activity of each muscle according to the support surface condition. Differences in the EMG activities between the bridging and unilateral bridging exercises, and between the right and left side were assessed using a paired t-test. The study showed that the EMG activities of all of the muscles were significantly higher when the bridging exercise was performed using the foam roll or sit-fit than on the firm surface. The EMG activities of the right rectus abdominis, right external obliques, the right internal oblique, and both erector spinae were significantly higher during unilateral bridging ex exercise using the foam roll or the sit-fit than on the firm surface. The EMG activities of all of the muscles were significantly higher during the unilateral bridging exercise than during the bridging exercise. Based on these finding, performing the unilateral bridging exercise using the sit-fit or the foam roll is a useful method for facilitating trunk muscle strength and lumbar stability.

This study aimed to examine whether McConnell taping for deltoid inhibition affects the Electromyographic (EMG) activity of shoulder girdle muscles during arm elevation. Ten young healthy men were randomly assigned to an experimental and control groups of five men each. For the experimental group, we performed taping for deltoid inhibition on the skin over anterior and posterior deltoids with non-elastic specific tape, and sham-taping with non-elastic under-tape for the control group. Surface EMG measurements were performed three times (before, during and after the tapings) at upper and lower trapezius, mid-deltoid, and serratus anterior muscles while elevating dominant aim with loading and unloading conditions. In deltoid inhibition taping group, there were significant differences in EMG activity of mid-deltoid (p<.05) and serratus anterior (p<.05) muscles during arm elevation with loading. During arm elevation without loading, the EMG activity was significantly decreased for MD in the McConnell taping group (p<.05). The findings indicate that deltoid inhibition taping can modify the activation patterns in shoulder girdle muscles as well as in deltoid muscle. in clinical setting. it may be effectively used for the management of patients with shoulder dysfunction.

대기 대순환 모형인 GCPS를 이용하여 북서태평양에서의 태풍 활동의 계절 예측 가능성을 조사하였다. 1979년부터 2003년까지 각 해에 대해 해수면 온도 관측 자료를 사용하여 5개월간 초기 조건을 달리한 10개의 앙상블 멤버를 적분하였다. 모형은 발생 빈도의 평균적인 월변화 경향과 발생 분포를 관측과 유사하게 모의하였으나, 발생 빈도의 경년 변화는 신빙성 있게 예측하지 못하였다. 이는 관측과 모형간 태풍 발생 빈도와 ENSO의 상관성 차이에 인한 것으로 실제 태풍 발생 빈도와 ENSO가 뚜렷한 상관 관계를 갖지 않는 것과 달리, 모형에서는 엘니뇨 시기에 평년에 비해 많은 태풍이 발생하고 라니냐 시기에 평년에 비해 적은 태풍이 발생하는 경향을 보였기 때문이다. 반면에, 관측과 모형 모두 ENSO와의 상관 관계가 높게 나타난 태풍 발생 경도의 경우에는 모형이 발생 경도의 경년 변화를 관측과 유사하게 모의하였다.

To improve trunk stability, various exercise protocols were introduced into the clinical field. Trunk and lumbar stability exercises on unstable surfaces are especially recommended to improve lumbar stability. The purpose of this study was to compare abdominal oblique muscle activity during leg raising in hook-lying position among 3 different type of surface conditions (on floor (F), vestibular board (VB), and foam roll (FR)). Sixteen able-bodied volunteers, who had no medical history of lower extremity or lumbar spine disease, were recruited for this study. Surface electromyography (EMG) activity was recorded from the internal and external oblique muscles of both sides. The normalized EMG activity was compared using a one-way repeated ANOVA. The results showed that the EMG activities of the internal oblique and external oblique of the lifted leg side during straight leg raising significantly increased under the FR condition when compared to the F condition. There was no significant difference of the EMG activity in abdominal oblique muscles between the VB and the FR conditions. The EMG activity of the internal oblique of supported leg side during the straight leg raising was significantly greater under the FR condition than the VB and F conditions (p<.05). The composition ratio of EMG activity of internal oblique muscles during straight leg raising was significantly increased under the FR condition. Therefore, straight leg raising exercise on foam roll in hook lying position could be beneficial to improve trunk and lumbar stability.

강 알칼리 이온수인 GC-100X의 항균력을 6가지 주요 식품위해 미생물들에 대해 시험하되 4℃, 25℃ 및 36℃의 서로 다른 온도조건과 유기물 조건 하에서 실험을 수행하였다. 그 결과 GC-100X는 37℃, 3시간 반응 조건 하에서 모든 유해균에 대해 1.0X 10^4 CFU/ml감소 이상의 효과를 나타내었으며 멸균 증류수나 표준 경수로 2배 희석한 경우에도 동일한 결과를 나타내었다. 유기물이 존재할 경우는 항균력의 약화가 관찰되었고, 그람 양성균에 대한 항균력은 높지 않았으나 그람 음성균에 대해서는 강력하고 빠른 사멸능력을 나타내었다. 방울 토마토에 인위적으로 E. coli O157:H7을 부착시키고 그에 대한 GC-100X의 세척효과를 기타의 세척제들과 비교해 본 결과, GC-100X 원액, GC-100X 5% 용액은 100 ppm 염소용액과 유사한 세척효과를 나타내었으며 시판 주방용 합성세제에 비해 그 성능이 우수하였다. 또한 시판 주방용 합성세제와는 달리 GC-100X, GC-100X 5% 및 3% 100 ppm 염소용액은 세척후 토마토 내부로의 균 침투를 억제하는 기능이 있음을 확인하였다. 이 결과는 식품 위생과 주방 청결에 일조할 하나의 방안으로서 안전성, 항균력, 세척력을 갖춘 GC-100X의 식품분야 적용 가능성을 시사하고 있다.

Biosurfactant have a low toxicity, good biodegradable, various structure. It can be used for a broad range of purposes in a large variety of different applications. Sorbitol mono alkyl ester synthesized by the Porcine Pancreatic lipase catalyzes transesterification reaction between a sugar alcohol and plant oils. We are examined the surface tension, emusion stability and critical micelle concentration were evaluated. Consequently It has a good properties of biosurfactant.

본 연구에서는 다양한 식품유해미생물에 대한 길항작용 및 항산화활성이 우수한 B. subtilis SRCM102046 균주를 식품보존 소재로서 이용하기 위해 반응표면분석법을 이용하여 균체 증량을 통한 항균활성 및 항산화활성 증대를 도모하고자 하였다. SRCM102046의 산업적 활용을 위한 성장조건을 최적화하기 위해 배양시간에 따른 균체 성장을 조사하였으며, 통계학적 기법인 반응표면분석법을 사용하였다. SRCM102046의 최적 성장을 위한 배지 성분을 선별하기 위해 Plackett-Burman design을 이용하였으며, PBD 결과 선별된 배지 성분으로 molasses, sucrose, peptone으로 예측되었다. 각 배지 성분의 최적농도를 결정하기 위한 방법으로 central composite design을 이용하였으며, 최종적으로 예측된 각 배지 성분의 농도는 molasses 7 g/L, sucrose 7 g/L, peptone 2 g/L로 예측되었다. 이때의 균체량은 22.03±1.30 g/L로 예측되었으며, 통계분석을 통해 실험모델의 적합성을 확인하였다. 또한, 실험 모델을 수행하여 건조균체량을 측정한 결과 22.02±0.35 g/L로 측정되어 실험모델에 의해 예측된 값이 오차범위 내에 존재하여 모델의 신뢰성이 매우 높음을 확인하였으며, 이는 실험모델에 의해 예측된 최적배지 사용시 최적화 이전의 LB 배지에서의 균체량(2.47±0.03 g/L)대비 약 9배의 균체량이 증가함을 확인하였다. 또한 최적배지에서 B. subtilis SRCM102046 배양 시 항균활성은 대조구로 사용된 LB 배지에서의 항균활성 대비 최대 140% 향상되었으며, 항산화활성은 약 100.41% 증가함을 확인하였다. 본 연구를 통하여 식품보존제로서B. subtilis SRCM102046의 산업화를 위한 배지최적화를 수행하였으며, 추후 박테리오신의 정제 및 특성 등에 대한 세부적인 연구가 필요하지만 본 연구를 통해 확립된 배양조건을 기반으로 식품보존 소재의 측면에서 매우 유용한 자료로 활용될 수 있을 것으로 기대된다.

열적외선 위성영상은 저비용으로 안전하게 지표온도를 추정할 수 있으며, 따라서 열적외선 위성영상을 활용하는 것으로 화산활동의 전조현상 모니터링이 가능하다. 그러나, 지표온도를 추정하기 위해 필요한 요소인 지표의 복사율 결정과 대기효과 보정은 쉽지 않기 때문에, 높은 정밀도를 기대하기 어렵다는 단점이 존재해왔다. 이에 본 연구에서는 최근 개발된 LSTD(Land Surface Temperature Difference)기법을 활용하여, 높은 정밀도의 지표온도 추정방법을 제안하고자 한다. 이 방법은 임의의 기준점으로부터 상대적인 지표온도의 차이를 구하는 것으로 대기효과를 보정하며, 복사율이 잘 알려진 식생지역에 한정적으로 적용함으로써 0.5K 이하의 높은 정밀도의 지표온도 추정이 가능한 것으로 알려져있다. 이 방법은 1) 대기효과 보정, 2) 식생지역의 추출, 3) 기준점의 지표온도로부터 상대적인 지표온도차 계산 및 4) 온도감률로부터 지형효과 보정의 단계로 이루어진다. 제안된 방법의 실험을 위하여, 화산활동이 활발한 아프리카의 Nyiragongo화산과 최근 전조현상이 발생하고 있는 백두산을 연구지역으로 선정하였으며, 열적외선 밴드를 탑재한 Landsat TM/ETM+ 영상을 수집하였다. 제안된 방법은 식생지역에만 적용되는 것으로서 공간적인 한계가 있으나, 높은 정밀도로 지표온도의 추정이 가능하기 때문에 화산활동의 전조현상을 관측할 수 있을 것으로 기대된다.

This study was conducted to establish the extraction conditions for the optimization of the biological activities of black garlic using the response surface methodology (RSM). The extraction conditions were based on the central composite design, with 15 kinds of variations in the extraction temperature (50-90℃), extraction time (3-15 hrs), and ethanol concentration (0-100%). The total phenol, flavonoids, thiosulfinate contents, and anti-oxidant activity of black garlic extract were significantly higher at the J condition (90℃ for 9 hrs with a 50% ethanol concentration). In this condition, the biological activities such as DPPH radical scavenging (66.10%), ABTS radical scavenging activity (75.02%), and reducing power by of FRAP (375.4 μM/mL) were excellent. Overall, the extraction conditions for the optimized biological activities of black garlic via RSM were expected to be at 89.68℃ for 9.79 hrs with a 55.72% ethanol concentration. The actual values were 96.4-114.8% of the predicted values.