Phosphine (PH3) fumigation has been widely used for controlling storedgrain insect pests, causing the development of resistance of stored-grain insect pests to phosphine. PH3 resistance in Sitophilus oryzae has been reported in Korea. However, PH3 resistance in Tribolium castaneum has not been reported yet. This study was conducted to determine susceptibilities of T. castaneum collected from five different domestic locations to PH3. The susceptibility to PH3 was investigated using the FAO fumigation method. All domestic T. castaneum individuals were controlled by PH3 at 0.04 g m-3. At 0.01 g m-3, T. castaneum collected from two domestic locations did not exhibit 100% mortality. A P45S point mutation in dihydrolipoamide dehydrogenase (dld) gene was found in a PH3-resistant strain of T. castaneum (Aus07), but not in five domestic stains or a PH3-susceptible strain (Aus10). No significant difference was found in dld or cyt-b5-r gene expression across all tested strains. However, the Gyeongju-collected strain of T. castaneum showed more than a 1.7-fold increase in cyt-b5-r expression compared to the Aus07 strain. cDNA sequence analysis revealed that P45S (C133T) in the dld gene was only present in Aus07. A characteristic single nucleotide polymorphism in the cyt-b5-r gene sequence was identified in the five domestic strains. This study suggests that it is necessary to continuously monitor PH3-susceptibility of T. castaneum in Korea to quickly identify resistant individuals and prevent the spread of PH3 resistance through rapid control.

Bacillus thuringiensis (Bt) is widely used as an environmentally friendly insecticide compared to chemical insecticides. However, challenges such as difficulty in direct practical application, limited efficacy duration, and stability have been identified. To solve these issues, formulation-based research is being extensively conducted. In this study, the high insecticidal activity strain Bt IMBL-B9, identified in previous research, was subjected to large-scale cultivation using a fermentor. Subsequently, various formulations were developed through formulation processes. and characteristics such as their wettability, suspensibility and particle size were assessed to select the optimized formulation.

한반도 남부지역에서 점토대토기문화는 청동기시대 후기에 중국 요령지역으로부터 유입된 문화로 유구와 유물에서 재지의 송국리문화와는 이질적이고 단절된 문화상을 보인다. 그러나 점토대토기문화 에서 확인되는 석기를 송국리문화의 석기군과 비교하면 유사점과 차이점을 모두 보이고 있어‘점토대 토기문화의 석기’에 대한 정체성은 명확하게 정의되지 않고 있다. 본고는 점토대토기문화 유적에서 출토된 석기 전반을 검토하고, 재지문화인 송국리문화와의 비교 분석을 통해 점토대토기문화 석기의 범주와 특징을 살펴보았다. 점토대토기문화의 석기는 송국리문화 에서 보이지 않던 새로운 기종의 출현은 확인되지 않지만, 새로운 형식의 출현은 확인되었다. 이러한 새로운 형식을‘점토대토기문화의 석기’로 설정하였다. 점토대토기문화의 석기상은 세 유형으로 분류하였다. Ⅰ유형은 자체적으로 고안한 형식으로, 무경 식석검, 삼각형석촉, 소형 편평양인석부, 단면 장방형의 석제 방추차 등이 있다. Ⅱ유형은 재지문화의 석기를 독창적으로 변형한 형식으로 구하부 직선의 유구석부, 편평유경촉 등이 있다. Ⅲ유형은 재지 문화의 석기를 그대로 수용한 것으로 합인석부, 편평편인석부, 구하부 사선의 유구석부 등이 있다. 이 세 유형은 점토대토기문화 집단이 새로운 환경에 적응하며 발현된 석기상으로 이를 통해 당시 환경에 적응ㆍ정착하는 과정을 유추할 수 있다. 석기상으로 볼 때, 점토대토기문화는 수렵에 비중을 둔 사회였음을 추정할 수 있다.

In this study, we introduce a novel TiN/Ag embedded TiO2/FTO resistive random-access memory (RRAM) device. This distinctive device was fabricated using an environmentally sustainable, solution-based thin film manufacturing process. Utilizing the peroxo titanium complex (PTC) method, we successfully incorporated Ag precursors into the device architecture, markedly enhancing its performance. This innovative approach effectively mitigates the random filament formation typically observed in RRAM devices, and leverages the seed effect to guide filament growth. As a result, the device demonstrates switching behavior at substantially reduced voltage and current levels, heralding a new era of low-power RRAM operation. The changes occurring within the insulator depending on Ag contents were confirmed by X-ray photoelectron spectroscopy (XPS) analysis. Additionally, we confirmed the correlation between Ag and oxygen vacancies (Vo). The current-voltage (I-V ) curves obtained suggest that as the Ag content increases there is a change in the operating mechanism, from the space charge limited conduction (SCLC) model to ionic conduction mechanism. We propose a new filament model based on changes in filament configuration and the change in conduction mechanisms. Further, we propose a novel filament model that encapsulates this shift in conduction behavior. This model illustrates how introducing Ag alters the filament configuration within the device, leading to a more efficient and controlled resistive switching process.

The towing distance, which is speed over the ground, and the water flow quantity, which is speed through the water, were used when estimating the amount of Metapenaeus joyneri resources that rose to the surface at night using the swept area method in order to compare and analyze the difference. It was conducted using a shrimp dredge, trial fishing gear for catching Metapenaeus joyneri. Catch during the entire survey period was 188.9 kg. Monthly catch ranged from 3.1 to 109.2 kg, highest in June and lowest in September. The swept volume calculated using the speed over the ground was about 13% higher than using the speed through the water. Metapenaeus joyneri resources estimated using the towing distance ranged from 320.1 to 14,649.8 kg. Resources estimated using the water flow quantity ranged from 278.5 to 12,886.3 kg. Therefore, the amount of Metapenaeus joyneri resources estimated using the speed over the ground was about 14% higher than the method using the speed through the water, indicating that the amount of resources was overestimated.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

본 연구는 특화된 문화관광지이자 주제관광의 대상이기도 한 한방식물원 탐방객의 인식 분석을 통해 한방식물원의 설계와 관리 방향을 제시하고자 한 것이다. 중국 하북성 안국시의 약박원(藥博園)을 사 례로 지각된 가치가 탐방객의 정서 및 편익에 미치는 영향을 밝히고자 AHP-엔트로피 가중치를 통해 한방식물원 탐방객의 지각된 가치 지표의 가중치를 계산하는 한편 요인분석을 통해 요인과 지표의 관 계를 탐색하였다. 본 연구에서는 경관평가 연구에서 일반적으로 적용하는 지표 가중치 분석방법을 탐 방객의 지각된 가치 척도 도구로 활용하였다. 그 결과, 자극 내부요인반응(S-O-R)이론의 관계를 바탕으 로 ‘탐방객의 지각된 가치’, ‘정서’, ‘지각된 편익’ 간의 관계를 설명하는 구조 방정식 모델에서는 다른 요인과는 달리 ‘관리 서비스 지각가치’는 탐방객의 지각된 편익에 유의한 정(+)의 영향을 미치지 않는 것으로 나타났다. 또한 AHP 계층분석법 및 엔트로피(Entropy) 가중치법을 통해 탐방객의 지각된 가치 지표에 관한 주·객관적 복합 가중치를 계산한 결과, ‘환경 지각 가치’의 가중치가 가장 높은데 반해 ‘관 리서비스 지각 가치’의 가중치는 가장 낮은 것으로 밝혀졌는데 이는 구조방정식의 상관관계 검정 결과 와 일치하는 것이다. 궁극적으로 한방식물원의 주변의 ‘환경’은 탐방객의 체험 향상에 가장 중요한 가 치임이 확인되었다.

This research investigated the immunoenhancing effect through the intracellular MAPKs and NF-B signaling pathways in macrophages activated by crude polysaccharides (YBP) of barley sprouts. YBP extracted from barley sprouts is composed of xylose (25.8%), arabinose (24.1%), galactose (23.4%), and galacturonic acid (11.7%). YBP did not affect the cytotoxicity and showed superior secretion of nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)- by RAW264.7 cells. Also, YBP dose dependently increased IL-6, TNF-, and inducible nitric oxide synthase (iNOS) mRNA gene expression. In the western blot, YBP strongly induced the phosphorylation of the p38, JNK, ERK, and IB pathways in RAW 264.7 cells. In the anti-pattern recognition receptor (anti-PRRs) assay, the effect of YBP on NO secretion strongly decreased toll-like receptor (TLR) 4 and Dectin1 antibodies, whereas IL-6 and TNF- secretion by YBP mainly decreased SR and CD14. Therefore, we concluded that YBPinduced NO, IL-6, and TNF- were secreted via the MAPKs, while NF-B pathways through TLR4, Dectin1, SR, and CD14 receptors existed in a macrophage surface and were involved in the immunoenhancing effect.

자생식물은 관상용, 약용, 식량자원으로 활용될 수 있는 잠재력을 지닌 고유 유전자원이다. 돌부추(Allium koreanum H.J. Choi & B.U. Oh)는 우리나라 해안 암반지대에 분포하는 자생식물 중 하나로, 기후변화와 서식지 감 소로 인해 보전 가치가 높은 식물이다. 이번 연구는 온도와 과산화수소가 돌부추의 발아에 미치는 영향을 조사하기 위해 수행되었다. 종자를 무처리(대조군) 또는 1% 과산화수소(H2O2)로 90분간 처리한 종자를 준비해 15, 20, 25°C 로 설정된 식물 생장 챔버에 배치하였다. 그 결과, 파종 23일 후 15°C에서 42%인 발아율이 20°C와 25°C에서 각각 18%와 0%인 발아율보다 2배 이상 높았으며, H2O2 처리 여부와 관계없이 15°C에서 발아율이 42%로 가장 높았다. H2O2 처리와 관계없이 최종 발아율 50%(T50)에 도달하는 일수는 20°C에서 가장 짧았지만, 일평균 발아율(MDG)은 15°C에서 가장 높았다. 따라서 1%의 H2O2 처리는 돌부추의 발아율에 큰 영향을 미치지 않았으며, 15°C의 온도가 돌부추의 발아율을 높이는 데 최적인 것으로 판단된다. 본 연구 결과는 돌부추의 발아를 위한 기초 연구 자료로 활 용될 수 있을 것으로 기대된다.

Stilbene-based fluorescent brighteners (FB) have been shown to enhance insecticidal activities of entomopathogenic viruses but little is known its effect on entomopathogenic bacteria. We investigated the effect of two FBs (FB 28 and FB 71) on the insecticidal activity of B. thuringiensis var. kurstaki (Btk) as well as the Lymantria dispar multiple nuclear polyhedrosis virus (LdMNPV) in Lymantria dispar asiatica. FB 28 increased the mortality at the combination with low concentration (1.6×102 IU/ml) of Btk, but FB 71 slightly reduced the mortality with middle and high concentrations (1.6×103 and 1.6×104 IU/ml) of Btk in comparison to Btk alone. Both FB 28 and FB 71 increased mortality in combination with LdMNPV at all concentrations (3×102, 3×104, and 3×106 POBs/ml) compared to LdMNPV alone. Our findings suggest that FBs enhanced pathogenic activities but depend on chemical nature of FBs.

1,2-Dichlorobenzene (1,2-DCB) is in various industries as solvents in herbicides, pesticides, and wax, and as degreasers or in the production of dyes. Studies on the hazards and risks of 1,2-DCB showed that this substance can cause skin corrosion, skin irritability, respiratory irritability, and certain target organ toxicity. Industrial workers are exposed to 1,2-DCB by inhalation or skin exposure and there is a lack of information on human hazards even though they can be exposed to organic compounds such as benzene or other DCB complexes rather than a single substance. In this study, we investigated the specific organ toxicity of 1,2- DCB and sex differences using whole-body inhalation in laboratory mice. Male and female mice were exposed to 0–120 ppm of the test substance for 13 weeks. After euthanization, the organs were collected, histopathological assessments and immunohistochemistry (IHC) were performed, and lipid peroxidation was analyzed. Macro and microscopic lesions were observed in the livers of male mice exposed to the test substance, and microscopic alterations were observed in the nasal cavities of male and female mice. IHC analysis of the liver confirmed a greater increase in cytochrome P450 induction in males than in female mice, and malondialdehyde and 4-hydroxynonenal were increased in both sexes by 1,2-DCB inhalation. Based on the relevant literature and experimental results, 1,2-DCB is believed to cause specific organ toxicity in the livers of male mice and the nasal cavities of both sexes of mice, which is supposed to be related to sex differences in cytochrome P450 induction and changes in lipid and oxidative products associated with the early metabolites of the test substance.

예이츠와 히니는 아일랜드의 호수와 바다에 서식하는 물고기와 어부의 이미지를 시적 글쓰기에 활용한다. 예이츠의 ｢물고기｣와 ｢어부｣는 삶의 희망과 불안을 썰물과 밀물로 탐구하며 삶의 지혜를 어부를 통해 낭만적으로 표현한다. 히니는 ｢송어｣, ｢농어｣, ｢연어잡이가 연어에게｣에서 바다와 호수의 생태적인 면과 이상 및 현실의 경계를 접목하여 해당 어류들이 사이를 치열하게 넘나든다. 히니의 물고기는 장어처럼 바다와 강의 경계를 상징하고 물과 흙 이미지의 결합인 진흙과 연결된다. 예이츠의 물고기와 어부는 경험과 지혜를 토대로 이상향을 향해 쌓아 올린 탑과 같다면, 히니의 물고기와 어부는 물과 흙의 경계에서 과거 기억과 현실, 그리고 시적 상상이 어우러져 균형을 유지하려는 수평기를 떠오르게 한다.

이 글은 예이츠와 디킨슨의 집을 프로이트의 언캐니의 관점에서 논의한다. 프로이트는 어떤 사건이나 개인의 심리적 경험인 언캐니의 개념을 단순히 신비한 것이 아니라 기묘하고 친숙하게 느껴지는 방식으로 구체화한다. 예이츠와 디킨슨에서 익숙한 집은 불안하고 섬뜩한 맥락에서 자주 마주쳐져 자아에 환원될 수 없는 불안감을 낳는다. 구체적으로 본고는 예이츠가 『연옥』에서 현실의 불안과 혼란, 절망의 상징으로서 집을 노인이 표상하는 방식, 그리고 디킨슨이 여성의 역할이 강요되고 억압되는 공간으로 제시하는 방식을 살펴본다. 그녀의 시에서 집을 매개로 자신의 과거를 은폐하고 억누르는 상황은 분열된 자아, 낯선 또 다른 나를 마주치게 한다. 예이츠와 디킨슨에게 언캐니 집은 억압되어 실현되지 못한 자아의 파편들이 잠복하여 출몰하고 의식의 경계가 모호해지는 공간이다.

Thermoelectric (TE) energy harvesting, which converts available thermal resources into electrical energy, is attracting significant attention, as it facilitates wireless and self-powered electronics. Recently, as demand for portable/wearable electronic devices and sensors increases, organic-inorganic TE films with polymeric matrix are being studied to realize flexible thermoelectric energy harvesters (f-TEHs). Here, we developed flexible organic-inorganic TE films with p-type Bi0.5Sb1.5Te3 powder and polymeric matrices such as poly(3,4-eethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and poly (vinylidene fluoride) (PVDF). The fabricated TE films with a PEDOT:PSS matrix and 1 wt% of multi-walled carbon nanotube (MWCNT) exhibited a power factor value of 3.96 μW ‧ m-1 ‧ K-2 which is about 2.8 times higher than that of PVDF-based TE film. We also fabricated f-TEHs using both types of TE films and investigated the TE output performance. The f-TEH made of PEDOT:PSS-based TE films harvested the maximum load voltage of 3.4 mV, with a load current of 17.4 μA, and output power of 15.7 nW at a temperature difference of 25 K, whereas the f-TEH with PVDF-based TE films generated values of 0.6 mV, 3.3 μA, and 0.54 nW. This study will broaden the fields of the research on methods to improve TE efficiency and the development of flexible organic-inorganic TE films and f-TEH.

The following is not a conversation with a bank clerk. " Instead, let me introduce you to customized credit loans," "Do you want me to connect you to the screen of using COVID-19 support funds and checking balance?" These are the contents of consultations with AI chatbots at financial institutions. Chatbot, which used to be an additional tool for adding convenience to life, is now at the center of our lives.