본 연구에서는 붕어를 이용하여 탁도 변화에 따른 여러 조직의 항산화효소 활성을 조사하였다. 탁도 50, 100, 150 NTU에서 사육기간에 따른 붕어 조직의 항산화효소 활성의 변화는 50과 100 NTU에서는 대조구에 비해 큰 변화가 없었으며, 고탁도인 150 NTU에서 비교적 두드러진 차이를 나타냈다. 붕어 조직의 항산화능을 DPPH 소거 활성도에서 보면 아가미, 간 조직에서 높은 활성을 보여 고탁도의 사육 조건에서 장기간 사육될 때 비효소적 항산화

본 연구는 우리나라 고유의 유전자원인 재래산양의 체내수정란 생산기술을 확립하고자 수행하였다. 흡입법(aspiration)과 세절법(slicing)에 의해 난소 한 개당 회수된 난자의 수는 3.9개와 4.1개를 나타내어 slicing방법이 aspiration방법보다는 많은 숫자의 난자를 회수하였으나 유의적인 차이는 나타내지 않았다. 회수된 난자의 등급별 분포는 aspiration방법에서 Grade I, Grade II, Grade III, Grade IV

This study was performed to investigate the effect of Lycii fructus beer on serum lipid profiles and antioxidant activity in rat Sprague-Dawley (SD) rats weighting about 190g were divided into the following 5 groups ; distillate water (Control), 5% ethanol in distillate water (Ethanol), commercial beer (CB), Lycii fructus beer (LFB) and 5% alcohol red wine diluted with distillate water (RW). Body weight, total food intake, FER and percent organ (liver, kidney) weight per body weight were not significantly changed by Lycii fructus beer drinking. After 6 weeks, serum total cholesterol, triglyceride and HDL cholesterol level were not significantly different. But, Lycii fructus beer intake tended to decrease serum triglyceride level and atherogenic index. Also, GOT and GPT levels were expressed lower than Ethanol group. There was not significantly different in hepatic glutatiione (GSH) content and glutathione-S-transferase (GST) activities among 5 groups. Lipid peroxidation in the hepatic was decreased by Lycii fructus beer intake. The results demonstrated that Lycii fructus beer was potential and effective antioxidant that can protect the decrease associated with alcohol.

식용대두유의 항온저장시 tryptophan과 arginine의 첨가농도에 따른 항산화 효과 및 기존 항산화제들의 항산화력에 대한 상승효과를 조사한 결과는 다음과 같다. 유지의 항온저장시 tryptophan과 arginine을 각 농도별로 첨가한 경우 모든 시료에서 항산화 효과를 나타냈으며 특히 tryptophan 1%를 첨가하였을 때 가장 효과가 좋았고 그 정도는 TBHQ를 첨가한 경우와 비슷하였다. 또한 tryptophan과 arginine첨가시료는 α-tocopherol보다 월등히 높은 항산화 효과를 보였다. tryptophan과 arginine을 α-tocopherol, ascorbic acid, citric acid와 혼합하여 대두유에 첨가했을 때 α-tocopherol과의 혼합물이 상승효과가 가장 높았다. 또한 citric acid나 ascorbic acid의 혼합물도 약하지만 상승효과를 보였다. 이상의 결과를 볼 때 tryptophan과 arginine은 유지에 대하여 항온저장시 모두 항산화 효과가 있었으며 특히 1%농도에서 가장 높게 나타났고 다른 항산화제와 병용했을 때 상승효과가 매우 우수하였음을 알 수 있었다.

Acornic powder was extracted with methanol and ethylacetate to obtain a crude acornic compounds. And they were examined concerning their antioxidant activities for linoleic acid. The results were as follows: 1. Hydrogen donating activity for DPPH was higher in 100ppm acornic compound than 100ppm BHT and 100ppm α-tocopherol. 2. When the 100ppm acornic compound was added to linoleic acid, which was heated at 50℃ for 48 hours, antioxidant activities by POV and TBA was higher than that of 100ppm α-tocopherol, but the effect was almost the same as the 100ppm BHT. 3. Antioxiodant activity of acornic compound showed synergistic effect along with malic acid, citric acid, tartaric acid, alanine, arginine, histidine, lysine-HCl, galactose, maltose, glucose and sucrose. 4. Acornic compound inhibited peroxidation of linoleic acid induced by heavy metals.

Methyl linoleate의 자동산화와 인지질에 작용하는 lipoxygenase의 활성에 미치는 탄닌산과 알파토코페롤의 영향을 HPLC로 조사하였다. 반응혼합물은 methyl linoleate 70mM, 라디칼 생성체 AMVN와 탄닌산 및 알파토코페롤 각각 0.7mM를 함유하였다. Hydroperxide 생성량을 측정하여 탄닌산과 알파토코페롤이 상당히 좋은 항상화제임을 알았고, 인지질 1μm에 탄닌산과 알파토코페롤을 각각1μm 함유한 혼합물에 효소를 첨가하여 활성 억제효과를 측정하여, 탄닌산과 알파토코페롤이 좋은 효소 활성 억제작용을 하고 있음을 알았다.

Background : The Kenaf (Hibiscus cannabinus L.) is an annual plant rich in phenolics compound and antioxidant activity. Particle size has an enormous effect on extraction of plant bioactive compound. Therefore, to evaluate the optimum particle size on extraction of bioactive compound from Kenaf was investigated. Methods and Results : Three kind of kenaf leaves; EF-1 and G-1 from Israel and Korea were collected in 2016 and the collected leaves were dried and pulverized are sorted by particle size. After adding 50 ㎖ of 100% ethanol per 0.5 ㎎ of the powder, the powder is extracted in an ultrasonic bath at 30℃ for 1 hour and then stirred for 1 hour at 250 rpm in the wise stirring. After extraction, the extract was filtered using filter paper. In the total polyphenol, flavonoid and antioxidant activity were higher in 100 to 200 ㎛ particle size of Gandae. On the other hand, phenolic compound, falvonoud and antioxidant were higher in 45 to 100 ㎛ particle size of EF-1 and G-1 Kenaf. Conclusion : Based on the results, the Israel varieties showed the best results compared to the Kenaf varieties of Korea. Among them, EF-1 had a particle size of 45 – 100 ㎛ Showed the higher antioxidant activity. It is believed that the smaller the particle size, the larger the specific surface area, which leads to an increase in the extraction efficiency of kenaf.

Background : The Kenaf (Hibiscus cannabinus L.) is an annual plant of African and Indian origin. and there are a lot of flavonoids in the leaves. To determine the most suitable method for extracting of Kenaf cosmetic ingredients, the data of changes in polyphenol, flavonoid contents and DPPH radical scavenging activities were analyzed, based on concentration of Extraction Methods. Methods and Results : 200 ㎖ of distilled water (100% ethanol in the case of ethanol extraction) was added to 20 g of the leaves of Kenaf; G-1 from Israel were collected in 2016 and the collected leaves were dried and pulverized, and five extraction methods were performed. The extraction method and conditions are as follows: pressurized high temperature extraction (AE: autoclave, 121 degrees, 15 minutes), ultrasonic extraction (UE: ultrasonic bath, 60 degree hot water, 1 hour), Hot water extraction (SE: spidulina mayina. 100 degrees 6 hours), Ethanol Extraction (EE: ethanol, spidulina mayina, 80 degrees 6 hours), room temperature stirring extraction (WSE: wise stirring, room temperature 250rpm 24 hours). After extraction, the mixture was filtered using a filter paper, and concentrated under reduced pressure at 40℃ for lyophilization. And diluted to 1,000 ppm with 80% ethanol. Conclusion : Based on the test results, EE method showed the highest results. In the case of using water as the solvent, the SE method showed the highest result. However, the results of the SE and AE methods are not significantly different from each other. Therefore, it is expected that if the extraction conditions of AE method are adjusted, the result of AE method will be higher than that of the SE method.

Background : The aloe has been reported to have significant therapeutic effects, such as inhibition of cancer cells' activation and proliferation as well as anti-inflammatory, anti-viral, and anti-oxidation properties. However, the phytochemicals analysis and antioxidant activity of the Aloe vera flower extracts have not been widely investigated. In this regard, we investigated the phytochemical profiles and antioxidant activity of ethanol extracts of the Aloe barbadensis flower in hydrogen peroxide-treated BALB/c mice. Methods and Results : This research highlights the phenolic constituents' profile and antioxidant activity of 70% ethanol extracts of Aloe vera flower for the first time. The ethanol-based extracts showed the inhibition for linoleic acid oxidation and free radical-induced DNA damage. Among about 11 phenolic constituents of the extract, identified by using high performance liquid chromatography (HPLC), the content of vanillic acid was highest, corresponding to strong antioxidant activities of the extract. The extracts elevated superoxide dismutase, catalase, and glutathione peroxidase enzymes activities in the liver tissue of hydrogen peroxide-treated BALB/c mice. The radical-scavenging activities of the extracts were well-correlated to the total phenolic content. Conclusion : In conclusion, Aloe bardadensis flower might be an effective source of natural antioxidant with high level of phenolic constituents.

Background : Collagen and elastin contribute to form a network under the epidermis and reduce the wrinkles. Collagen and elastin are degraded by collagenase and elastase. Therefore, inhibition of collagenase and elastase activity could be an excellent method for anti-wrinkle. Pinus Koraiensis leaves contain various flavonoid compounds such as quercetin and kaempferol. According to previous reports, kaempferol has increases procollagen synthesis and inhibitory effect on MMP-1 activity. This study was performed to investigate the anti-wrinkle effect of Pinus Koraiensis leaves extract and fractions. Methods and Results : The active components of Pinus Koraiensis leaves extracts and fractions were determined. Total phenol and total flavonoid contents measured using the Folin-Ciocalteu reagent and according with aluminum chloride colormetric method. The kaempferol content analysis using HPLC system. The total phenol, flavonoid and kaempferol contents of ethyl acetate fraction was the highest. The antioxidant activity measured in according with DPPH, ABTS and RP assay. Pinus Koraiensis leaves extract and factions were the highest ethyl acetate fraction in antioxidant activity. The inhibitory activity on collagenase and elastase of Pinus Koraiensis leaves extract and factions were determined. The results of inhibitory activity on enzymes associated with wrinkle formation was the highest of n-butanol fraction, the next higher was 80% ethanol extract. As a result of using MMP-1 content and procollagen type-I C-peptide content assay kit, the anti-wrinkle effect of n-hexane fraction was highest. Conclusion : These results indicate that Pinus Koraiensis extract may help anti-wrinkl

This study was performed in order to determine the effect of roasting (160 for 0, 30, 60, 90 min) on the physicochemical characteristics and antioxidant activity of hot water extracts of dried Liriope platyphylla. With respect to Hunter’s color value, lightness and yellowness decreased roasting time increased. The difference (ΔE value) in lightness, redness and yellowness was 15.88 and 22.94 for 30 and 60 min. The 30 min roasted sample contained more soluble solid content (12.7 °Brix) than other samples. The pH and acidity of the 60 min roasted sample were 5.06 and 0.14%, respectively. The highest contents in reducing sugar and total phenolic compounds contents was obserned in the 60 min roasted sample (17.68, and 35.01 g/100 g, respectively), and flavonoid content was the highest in the 90 min roasted sample (10.53 mg/100 g). The crude saponin content of the 90 min roasted sample (21.90 mg/100 g) was higher than that of others samples (8.0-15.36 mg/100 g). Moreover, ginsenosides such as Re, Rg3, and Rh2, were detected, among which the Rh2 content was the highest. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (31.64-53.72%), 2,2'-azinobis(3-ethylbenzothiazoline)- 6-sulfonic acid (ABTS) radical scavenging activity (61.59-96.71%), and the ferric reducing antioxidant power (72.38-183.11%) were higher in the roasted samples than in the control group. As a result, we confirmed that roasting increased the amount of active compounds, which increased the yield of useful extract.

본 연구에서는 영양학적 가치가 높은 쌀귀리의 식품산업 이용률을 높이고자 쌀귀리를 입도별로 제조하여 이들의 이화학적 특성 및 항산화 활성을 비교하였다. 쌀귀리의 조 단백질, 조지방 및 조회분 함량이 각각 15.7%, 10.0% 및 1.8%이었으며, 입도가 작아질수록 이들의 함량은 감소하였다. 주요 유리아미노산은 비필수아미노산 중에서는 cystine(58.3 mg%), β-aminoisobutyric acid(53.8 mg%) 등, 필수아미노산 중에서는 phenylalanine(8.6 mg%)과 histidine (12.2 mg%)이었으며, 입도가 작아질수록 함량은 감소하였다. 총 전분, 아밀로스, 손상전분, 총 식이섬유, β-glucan 및 총 유리당 함량은 각각 56.4%, 21.4%, 11.7%, 11.0%, 4.7% 및 2,555.3 mg%이었으며, 입도가 작아질수록 총 전분, 아밀로스 및 손상전분의 함량은 감소한 반면에 총 식이섬유 와 β-glucan의 함량은 증가하였다. 색도는 L 값이 75.3, a 값이 0.3 및 b 값이 13.1이었으며, 입도가 작아질수록 L 값은 증가하였으나 a와 b 값은 감소하였다. 호화특성은 최고점도가 263.8 RVU, 최저점도가 155.6 RVU 및 최종점도가 399.3 RVU이었으며, 입도가 작아질수록 최고점도와 최종점도는 증가하였다. TPC, TFC 및 항산화 활성(DPPH radical assay, ABTS radical cation assay 및 reducing power) 은 각각 237.8 μg/g, 90.9 μg/g, 57.3%, 53.4% 및 0.23이었으며, 입도가 작아질수록 항산화 성분 및 항산화 활성은 감소 하였다. 한편 TPC와 TFC에 관한 각 항산화 활성의 결정계수(correlation determination coefficient, R2) 값이 각각 0.87 이상과 0.81 이상으로 나타나 항산화 성분과 항산화 활성은 상관성을 보였다.

Hericium erinaceus is considered a functional food and potential medicinal source. The present study was conducted to examine the potential antioxidant and anti-inflammatory activities of carried out with water and ethanol extracts of Hericium erinaceus grown on germinated green rice (HEGR-W and HEGR-E, respectively) and the water and ethanol extracts of germinated green rice (GR-W and GR-E, respectively) as potential medicinal resources or antioxidant and anti-inflammatory agents. Methods and Results: The total phenolic and flavonoid contents, DPPH, and ABTS activity, reducing power, DNA protective activity, cell viability, and NO production were investigated. The total phenolic and flavonoid contents were highest in HEGR-E (66.53 ± 2.40 ㎎·GAE/100 g and 82.12 ± 7.10 ㎎·CE/100 g respectively). HEGR-E exhibited high DPPH (44.70 ± 1.28%) and, ABTS (44.70 ± 1.28%) activity and reducing power (0.219). HEGR and GR extracts showed protective activity against DNA damage. The cytotoxicity of HEGR and GR in RAW264.7 cells and LPS-induced RAW264.7 cells was low. HEGR-E and GR-W exhibited anti-inflammatory effects through a 28% inhibition of NO production in LPS-induced RAW264.7 cells. Conclusions: These results suggested that the extracts of Hericium erinaceus grown on germinated green rice could be a potential medicinal material with natural antioxidant and NO inhibitory properties.

Background: Senna tora is a flowering plant in the legume family Fabaceae. Its seeds are roasted and consumed as tea in Asia, to reduce inflammation in the liver and improve eyesight. Thus, it has been considered as an important medicinal crops in Asia. However, breeding trials to improve its genetic properties are rare. Mutation breeding by gamma ray is known to be an effective and highly successful approach for the generation of agronomically useful cultivars. Here we analyzed the effects of several dosages of gamma ray on the biological conditions of Senna tora seeds. Methods and Results: The germination rate and growth patterns of Senna tora were examined following irradiation with gamma ray at 100, 200, 300 and 400 Gy. The total phenolic compound contents and antioxidant activities of Senna tora were analyzed. Germination increased at 100 and 200 Gy in the M1 and M2 generations compared with that of the control (M0). The total phenolic compound contents and antioxidant activity of the seeds significantly decreased as the radiation dosage increased above 100 Gy in the M1 generation. Conclusions: Senna tora, irradiated with gamma ray at dosages 100, 200, 300, and 400 Gy, showed maximum germination rate at 200 Gy in the M2 generation. Plant height and leaf size gradually decreased with increasing gamma ray intensity in the M2 generation. The total phenolic compound contents decreased significantly at 400 Gy, and the related antioxidant activity was also decreased as the radiation dosage increased.

황금(Scutellariae baicalensis)은 항염 작용이 뛰어나 예로부터 사용되어온 약재로, 본 연구는 인삼에서 분리한 유산균 Leuconostoc mesenteroides (L. mesenteroides)을 이용해 황금 발효물을 제조하고 항산화와 미백 효과를 조사하였다. 황금 발효물은 황금을 70% 에탄올로 추출한 후에 L. mesenteroides를 접종하여 발효 제조하였다. 발효 전 황금과 황금 발효물에서 2가지 지표 성분 baicalin과 baicalein을 high-performance liquid chromatography (HPLC)을 이용하여 retention times (tR)과 UV spectra를 확인함으로써 정성 및 정량 분석하였다. 세포 생존율 실험 결과, 발효 전후 황금 모두 독성이 확인되지 않았고 DPPH 라디칼 소거능 실험은 발효물의 SC50 값이 34.43 μ g/mL로 발효 전보다 우수한 효능을 나타내었으며, 세포 독성을 나타내지 않는 농 도에서 흑색종 세포인 B16F10을 이용한 melanin 생성 억제 활성 실험 결과, 황금 발효물은 우수한 melanin 생성 억제 효과를 나타내었다(IC50 = 68.17 μ g/mL). 이상의 결과들로부터 황금 발효물이 항산화 효능뿐만 아 니라 미백 효능을 갖는 화장품 원료로서 개발 가능성이 있음이 시사되었다.