A 12-year-old intact female Schnauzer was referred for the evaluation of poorly controlled diabetes mellitus: despite insulin therapy, blood glucose concentration was consistently high, indicating a decreased insulin sensitivity. Laboratory analyses revealed persistent hyperglycemia, glucosuria, and ketonuria. Diagnostic approaches were performed to identify concurrent disorders that can cause insulin resistance. The dog was found to have concurrent hyperadrenocorticism, hyperlipidemia, pancreatitis, and vaginal cytology indicating diestrus in the estrus cycle. Trilostane administration for hyperadrenocorticism improved the insulin response; however, the dog remained hyperglycemic. Eventually, the dog showed complete remission without insulin administration 1 week after the ovariohysterectomy. The dog remained in remission for approximately 4 months, but eventually relapsed and the condition was permanent. Diestrus in intact females and hyperadrenocorticism are known to be the two main causes of insulin resistance in dogs. After the management of these conditions, the dog achieved diabetes remission, which rarely achieves in dogs. In cases of insulin resistance, such as hormonal imbalances or inflammatory conditions, remission can be achieved by addressing the underlying cause. Hence, it is important to assess the presence of comorbidities associated with insulin resistance in dogs with poorly controlled diabetes mellitus and to treat each condition as soon as possible.

A one-year-old, intact male Maltese was referred with dehydration, anorexia, and marked hyperglycemia. The dog had been managed due to meningoencephalitis of unknown etiology (MUE) for three months. The dog had been treated with long-term prednisolone administration. Diabetic ketoacidosis (DKA) was identified based on the blood chemistry and venous gas analyses, and intensive treatments including insulin administration were initiated. On further examinations, there was no any other disease that contributed to the occurrence of DKA. Insulin resistance resulted from the administration of prednisolone was highly suspected, but the agent could not be tapered due to managing MUE. Following resolution of DKA, the dog was discharged with life-long insulin and prednisolone therapy. Over the next two years, the dog continued to be routinely re-evaluated and was managed with permanent insulin therapy (0.8–1.4 units/kg SC 12 hourly) and medications including prednisolone (0.4–1.1 mg/kg PO 12 hourly). Because MUE severely progressed, the dog was euthanized by owner’s request. Histopathologic examination of pancreas obtained by post-mortem revealed that both endo- and exocrine pancreas was within normal limit. The case described herein showed the risk of ketoacidosis as well as hyperglycemia after long-term prednisolone administration in a dog without pancreatic islet pathology.

본 연구는 스트렙토조토신에 의해 유도한 당뇨병, 알코올성 간 손상 실험용 랫드에서 혈액 지질 감소, 당뇨병 및 알코올성 간 손상 예방에 미치는 지장김치 추출물의 경구투여 효과를 조사하였다. 당뇨병 모델동물에서, 혈액 지질 프로파일, ALT, AST 수준은 DC (당뇨병 대조군)와 비교할 때 김치추출물 투여군에서 낮아졌으며 혈당은 DCJK (DC+지장김치 추출물 경구투여군)가 DCCK (DC+일반김치 추출물 경구투여군)에 비해 낮았다. 인슐린 수준은 NC (정상대조군), DCJK > DCCK > DC 순서로 높게 나타났다. 알코올성 간 손상 모델동물에서, 혈액 ALT, AST, 빌리루빈 농도는 AC (알코올 대조군, 일일 소주 1병 섭취군) > ACCK (AC+일반김치 추출물 경구투여군) > ACJK (AC+지장김치 추출물 경 구투여군) > NC 순서로 낮았다. 간 조직의 임상병리학적 소견은 AC에서 가장 심각한 손상을 보였으나 소주1병과 김치 추출물 특히, 지장김치 추출물 경구투여군은 일반김치 추출물 경구투여군에 비해서 회복되는 속도가 개선되었다. 결과는 당뇨병 및 알코올성 간 손상 모델동물에서 지장김치의 섭취가 혈액 지질 프로파일, 혈당, ALT, AST 수준을 낮추고 인슐린 분비능력을 개선하여 줌으로써 항당뇨 및 알코올성 간 손상 보호효과를 갖는다는 사실을 보여준다.

본 연구는 만 65세 이상 85세 이하의 제2형 당뇨병 비만 여성 노인을 대상으로 12주간 복합운동 후 irisin, 신체조성 및 당 대사에 미치는 영향을 구명하기 위하여 복합운동군(n=20), 대조군(n=16)으로 구분하여 실시하였다. 복합운동 프로그램은 야외 걷기 운동과 탄성 밴드 운동을 12주간 주 3회, 1회 60분 간 실시하였으며, 야외 걷기 운동의 운동강도는 중강도(RPE 5~6)로 수행되었으며, 탄성 밴드 운동은 1~4주는 저강도(OMNI-RES 3~4), 5~8주는 중강도(OMNI-RES 5~6), 9~12주는 고강도(OMNI-RES 7~8)로 점진적으로 운동강도를 증가시켰다. 그 결과 복합운동군의 irisin이 유의하게 증가하였고(p<.001), 체지방률은 유의하게 감소하였으며(p<.001), 골격근량은 유의하게 증가하였다(p<.001). 당 대사 관련 인자 중 HbA1c(p=.020), 혈당(p<.001)은 유의하게 감소하였고, HOMA-β는 유의하게 증가하였다(p<.001). Irisin의 변화는 체지방률의 변화와 부적 상관이 있는 것으로 나타났고(r=-.423, p=.010), 유산소성 지구력 변화와 정적 상관이 있었다(r=.355, p=.034). 또한, HbA1c(r=-.351, p=.036)와 혈당(r=-.424, p=.010)의 변화는 부적 상관이 있었으며, HOMA-β는 정적 상관이 있는 것으로 나타났다(r=.411, p=.013). 결론적 으로 규칙적인 복합운동의 실천은 제2형 당뇨병 비만 여성노인의 혈중 irisin의 수준을 증가하고, 신체조성 변화에 긍정적인 영향을 주는 것으로 나타났다. 그리고 당화혈색소, 공복혈당 및 인슐린 분비능을 개선하여 당 대사 조절 능력에 도움을 주어 당뇨 예방 및 관리를 위해 효과적인 운동으로 권장될 것으로 사료된다.

This study includes the clinical features of diabetic patients with fascial space infections of head and neck and those of non-diabetic patients. We retrospectively studied 62 patients who were treated for fascial space infections of the head and neck, and compared the patients’ background, the aetiology of the infection, clinical variables, laboratory values, and treatment process. Statistical analyses of the differences between groups were made with Student's t test, the chi square test, and analysis of variance, as appropriate. Diabetic patients had more spaces involved, had longer hospital stays, and developed more complications. The main microorganism of diabetic group was Klebsiella pneumonia and otherwise, Streptococcus spp. was for non-diabetic group. We conclude that diabetic patients are more likely to develop complications and it is necessary to consider about dominant microorganism when using medication for diabetes patients

Superovulation is a technique to acquire rats to produce many eggs than normal rats. Superovulated eggs were used to make cloned animals through somatic cell nuclear transfer technology (SCNT). Healthy and valuable oocyte retrieval is essential for successful somatic cell nuclear transfer. Superovulation is also essential to maximize to the yield of IVF-derived rat eggs. Osmotic pumps (Alzet®) are miniature in order to provide research with a convenient, and reliable alternative to chronic injections. Acquiring superovulated oocytes through osmotic pump in minimizing irritation to the uterus and ovaries are competitive. We investigated the effects of pregnant mare serum gonadotropin (PMSG) and human Chorionic Gonadotropin (hCG) using osmotic pump in sDM rat. Adult female rats at 11 wks of age were used for superovulation. The response to PMSG and hCG were examined by osmotic pump of 150 IU/kg PMSG + 75 IU/kg hCG or 150 IU/kg PMSG + 150 IU/kg hCG or 300 IU/kg PMSG + 150 IU/kg hCG or 300 IU/kg PMSG + 300 IU/kg hCG. HCG was administered 48 hrs later after administration of PMSG. Oocytes were collected from the oviducts 16–18 hrs after hCG administration. Superovulation was significantly higher in rats administrated 150 IU/kg PMSG + 75 IU/kg hCG. This study demonstrated that healthy oocytes were produced in DM rat by PMSG and hCG that flowed through the osmotic pump ameliorate on uterus and ovaries.

Several species show low sensitivity to IVM, and the development of optimized medium possible oocyte quality and stable growth. Furthermore, adding additive to the medium can effectively reducing development cost and leads to easy handling of oocytes. Isoliquritigenin and formononetin are extracts found in licorice. Previous studies reported that isoliquritigenin and formononetin affected the activity of sperm, but the oocytes are unknown. This study adds isoliquritigenin or formononetin to αMEM to mature oocytes under simple IVC conditions. Recovered oocytes are cultured in αMEM, isoliquritigenincontaining medium and formononetin-containing medium. In study we proved that in addition to the medium, above the quality of oocytes cultured when specific additives were added, more stable growth is possible. collection and IVM of oocyte. SD rats at 6 to 8 wks of age are injected is intraperitoneal with 30 IU/mL of PMSG and 48 hrs later, HCG 50 IU/mL is intraperitoneal injected. Oocytes are collected ovary after 17 hrs. Collected oocytes are cultured for 16 hrs with 200 μL αMEM and 200 μL αMEM containing isoliquritigenin or formononetin at 0, 0.01, 0.02, 0.04, 0.1 mg/mL. Also, isoliquritigenin and formononetin were mixed with 200 μL αMEM at a ratio of 0.25: 0.75, 0.50: 0.50, and 0.75: 0.25 mg/mL respectively. Oocytes supplemented with isoliquritigenin and Formononetin had high quality than oocytes cultured with αMEM and showed an increase in the IVF fertility rate. Our experimental results indicate that using isoliquritigenin, formononetin when cell culture, rather than used only in medium, more effective oocyte quality and stable growth.

Insulin detemir is a long-acting basal insulin analogue recently introduced in veterinary medicine for treatment of canine diabetes mellitus. As there are only limited studies in dogs, long-term evaluation of insulin detemir in veterinary medicine is required. In this study, we investigated trends in12-hour blood glucose concentration during hospitalization and evaluated initial and following doses of insulin detemir for several months in six diabetic dogs. The mean levels of blood glucose over 12-hour periods were between 113.5 to 327.2 mg/dL, and the average glucose nadir was 103 mg/dL in the six dogs. The dogs were treated with a mean dosage of 0.24 U/kg of insulin detemir, but hypoglycemia was observed in four of the dogs at the first monthly follow-up. Thus, insulin doses were adjusted according to the nadir levels of glucose observed during the follow-up periods (range, 1 to 16 months). The total range of insulin doses throughout the study period was between 0.1 and 0.4 U/kg. Changes in insulin doses in each dog during the follow-up period were not variable. We suggest that insulin detemir might be not only an alternative choice against traditional insulin for patients with insulin resistance or concurrent disease but also an effective home therapy medication in canine patients with DM. This study could help inform veterinary practitioners regarding the use of insulin detemir for canine insulin-dependent DM.

A 10-year-old castrated male Korean shorthair cat weighing 4 kg was referred with signs of insulin-resistant diabetes mellitus based on clinical signs of polyuria, polydipsia, and polyphagia. Diagnosis of pituitary-dependent hyperadrenocorticism (PDH) was made based on results of an adrenocorticotropic hormone stimulation test and a dexamethasone screening test. In addition, plasma concentrations of insulin-like growth factor 1 (IGF-1) increased. Radiography, ultrasonography, and computed tomography (CT) revealed hepatomegaly, renomegaly, and adrenomegaly affecting both adrenal glands as well as multiple cysts in a generally enlarged pancreas. Magnetic resonance imaging (MRI) showed that the cat’s pituitary gland was enlarged. The pituitary gland had a predominantly unilateral extension to the left. The signal intensity of the pituitary gland on precontrast T1 weighted images was hypointense compared to that of soft tissue and hyperintense compared to that of cerebrospinal fluid. On T2 weighted images, the pituitary gland was predominantly hypointense with a hyperintense rim. Contrast enhancement of the pituitary gland was not evident, and a mild degree of ring-like enhancement was seen. In addition, mild peritumoral edema was present. This is the first report of a cat with suspected double adenoma of the pituitary gland on the basis of compatible clinical signs, increased serum IGF-1 concentration, PDH, CT images, and MRI findings in diabetic cats with insulin resistance.

Century-old nutrition and health concepts can be revived and applied in the modern age in the forms of newly developed menus, recipes, and lifestyle education. Current medical nutrition therapy concepts were first described in the Chosun Dynasty (1392-1897) in Korea based on the philosophy that food and medicine originate from the same source, which is known as ‘food as medicine’. Recognizing the importance of culture, tradition, local diet, and lifestyle on health and medical nutrition therapy, we tried to rediscover traditional Korean approaches towards food consumption and nutrition through systematic review of the literature and developed contemporary menus accordingly. The medical nutrition therapy prescriptions described in ‘Shikryochanyo’ (1460) by the Chosun Dynasty’s royal physician Soonyi Jeao cover 45 different diseases. In this project, we developed contemporary menus for those disease models that are most prevalent in modern society. Menus developed with foods that are readily available today were evaluated for their nutritional content and adequacy using a computer-aided nutritional analysis program (CAN pro 3.0, developed by the Korean Nutrition Society for comparison with RDA for Koreans). Therefore, century-old nutrition and health concepts can be revived and applied in modern society as newly developed menus recipes and lifestyle education.

This study compared the nutrient intake of obese versus non-obese non-insulin dependent diabetes mellitus (NIDDM) patients for Diabetes Medical Nutrition Therapy. The study was conducted at medical hospitals in Gyeonggi and Seoul from April 2009 to November 2009. Fifty-six adult male NIDDM patients were enrolled and divided into two groups: 36 into an obese group (BMI ≥25) and 20 into a non-obese group (BMI〈25). To conduct this study, anthropometric measurements, and daily nutrient intake of obese and non-obese NIDDM patients were measured. Daily nutrient intake was estimated by 24hr-recall and analyzed by the CAN program. In the results, anthropometric measurements of the two groups showed significant differences in weight and BMI (p〈0.001). Daily nutrient intake of the two groups showed no significant differences, except for vitamin E intake (p〈0.05). The total energy intake of the non-obese and obese groups were 2,669.9±964 kcal and 2,555.4±803 kcal, respectively, which were both above 113% of the recommended Dietary Reference Intakes for Korean (KDRIs). Cholesterol and sodium intake were 378.1±215.6 mg and 6,478.9±2755.1 mg, respectively for the non-obese group. Cholesterol and sodium intake were 308.1±155.6 mg and 6,306.8±2788.9 mg, respectively, for the obese group. Both groups were above 150% of the recommended levels set by the Korean Diabetes Association (KDA). However, their antioxidant nutrient intake was appropriate. Meanwhile, their fiber intake was 10.7±5.1 g and 9.8±5.2 g, respectively, which was lower than 40% of the recommended intake set by the KDA. The results show that the nutritional education for obese and non-obese NIDDM male patients must aim to reduce total energy, cholesterol, and sodium intake, while increasing fiber intake. In addition, the factors related to a patient's glycosylated hemoglobin, serum lipids, blood pressure, and weight change must be calibrated for the appropriate energy, fat, cholesterol, sodium, and dietary fiber intake.

Anti-diabetic activities of cultured mycelia from Ganoderma applanatum are being evaluated in this study. The OGTT and 4-weeks of repeated oral efficacy tests are conducted in mice at the doses of 0 (vehicle treatment), 25, 75 and 225 ㎎/㎏/day, respectively. In human study, the test article was administered orally every day for 8-week at a dose of 1,500 ㎎/㎏, tid and placebo group. The blood glucose levels (BGL) at 0.5 hour after treatment are significant decreased in all treatment groups of OGTT test. In the 4-week test, BGL of 75 and 225 ㎎/㎏/day group is continuously decreased during all treatment periods and the BGL of 25 ㎎/㎏/day group show decreasing trends at the final week, the pancreas weight of all treatment groups are being increased, and the Langerhans-islet numbers were increased at all treatment groups with a dose-response manner. There are no test article-related abnormal signs and the fasted blood glucose (FBG), postprandial blood glucose (PPG) and HbA1c are decreased significantly after 8-week treatments. These results that the cultured mycelia from Ganoderma applanatum could decrease BGL by protecting the degeneration of Langerhans islets.

본 연구는 동충하초(Isaria tenuipes)를 유전적 당뇨 질환 동물모델인 OLETF 랫드에서 4주간 경구 투여하여 항당뇨 효과에 대한 연구결과로써, OLETF 랫드 동충하초(Isaria tenuipes) 투여군은 당뇨대조군과 비교하여 투여 4주에는 사료 섭취량과 체중이 증가하였다. 이는 동충하초(Isaria tenuipes)를 섭취한 OLETF 랫드는 고지방식 사료만 섭취 한 OLETF 랫드에 비해 당뇨 유발이 개선된 결과로 판단 된다. 혈중 포도당 농도, 인슐린 농도 및 인슐린 저항성 지표인 HOMA-IR 수치가 용량 의존적으로 감소하였다. 또한 GLUT4 유전자 발현을 측정한 결과 대조군에 비해 동충 하초(Isaria tenuipes) 투여군의 mRNA 및 단백질이 용량의존적으로 증가하는 경향을 보였다. 따라서, 동충하초 (Isaria tenuipes)의 섭취가 제2형 당뇨에서 인슐린의 감수성이나 저항성에 영향을 미치고, 혈당을 강화시키는 것으로 사료되므로 당뇨병환자의 혈당저하에 기여할 것이라 기대한다. 이에 본 연구는 천연물질을 이용한 당뇨병의 예방 및 치료에 동충하초(Isaria tenuipes)를 사용할 수 있는 이론적 토대를 제공할 것으로 생각된다.

A 10-year-old intact female Cocker Spaniel weighing 15.3 kg presented with dysbasia. The dog had hyperglycemia and glucosuria at presentation. Neurological examination and magnetic resonance imaging on the following day indicated the onset of cervical disk disease. Four days later, the patient returned due to sudden vomiting and anorexia with severe dehydration. Abnormal laboratory findings included severe hyperglycemia with ketonuria. Plasma progesterone concentration was very high at presentation. She was hospitalized for critical care, and insulin administration with fluid therapy was initiated. Mean blood glucose concentration showed a gradual decline, and hyperglycemia with glucosuria disappeared four days after initiation of insulin therapy. This case demonstrated that high plasma progesterone concentration during diestrus can contribute to development of diabetes mellitus in intact female dogs.

Phosphoprotein Enriched in Astrocytes (PEA15) is a 15kD-sized intracellular signaling protein, highly expressed in astrocytes and constitutively expressed in peripheral tissues. Recently it was found that PEA15 expression was elevated in patients suffering type 2 diabetes and suggested to be involved in the syndrome of insulin resistance. To investigate the functional role of PEA15 for the control of blood glucose level, we produced a transgenic pig over-expressing mouse PEA15 (mPEA15). As a model animal, pig has many advantages. They have a higher fecundity and a short generation time and are physiologically similar to human. Using the transgenic pig, we carried out a series of experiments to establish a link between PEA15 expression and the insulin resistance. Our results suggested that, compared with control pig, mPEA15 pig has, (1) a higher blood resistin level, (2) a lower cell membrane-embeded GLUT4 level, and (3) a lower glucose clearing ability based on oral glucose tolerance test (OGTT). When our results combined, it can be concluded that mPEA15 over-expressing pig has many symptoms of insulin resistance and these pigs will become a useful disease model to investigate diabetes mellitus in the near future.

The purpose of this study is to compare the correlation of clinical characteristics and patterns of disease. Subjects of the study were the adults(207) living in Geoje City, the diabetes mellitus and the hypertension patients(166) and the normal people(41). In the diabetes mellitus group and the hypertension group, blood pressure, blood glucose, total cholestero LDL-cholesterol and atherogenic index(AI) were significantly high. As the obesity index was getting higher, the blood pressure of the diabetes mellitus group was high, and the HDL-cholesterol of the hypertension group was low, but AI of it was significantly high. The AI was significantly high as serum lipid index were getting higher in both groups. The rate of the prevalence was very high in the diabetes mellitus group(74.3%) and the hypertension group(73.7%). The pattern in the diabetes mellitus group was in order of the hypertension, the hyperlipidemia, and the obesity but, in the hypertension group was the hyperlipidemia, and the obesity. The obesity index and serum lipid index of complex patient group were higher than single patient group.

Oxidative stress is believed to play an important role in the development of vascular complications associated with diabetes mellitus. We examined the antioxidative effect of sesamin and sesamolin on the preventing the development of insulin dependent diabetes mellitus using streptozotocin-induced Sprague Dawley diabetic rats. From 48 hours after injection of streptozotocin (60 mg/kg body weight), a portion of diabetic rats were fed with 0.2% sesamin and sesamolin containing diet for 3 weeks. There were significant differences of blood glucose and kidney weight between diabetic ports and control. Sesamin and sesamolin increased glutathione-S-transferase activity in kidney. The concentration of the thiobarbituric acid-reactive substances in the serum, liver, and kidney of diabetic rats administered sesamin and sesamolin decreased significantly as compared with that of the non-treated diabetic group. Dietary sesamin and sesamolin suppressed the oxidative stress in the diabetic rats. These results demonstrated that sesamin and sesamolin are potential and effective antioxidants that can protect the complications associated with diabetes.

A dietary treatment of Chinese medicine for diabetes mellitus was extensively studied and compared with that of western medicine. The main results are as follows: Chinese medicine is based on the following empirical dietetics. First, likeness helps likeness. When an internal organ of mankind is not functioning properly, the food or drug from the same organ of an animal will be helpful to cure it. Second, take good foods for health properly and never take any foods against body. Third, building up one's body by taking tonics is not quite the same as building up one's body through proper eating. On the other hand, western medicine is based on the experimental and scientific methods which are modernized in accordance with the development of science and technology. It emphasizes upon finding the cause of a disease. Then this disease can be cured by doing a necessary medical treatment which sometime uses a surgical operation or chemical and radiological method or both. Although there are many ways in treating a diabetic in Western Medicine, here the diabetics is supposed to be the best. The same is true for Chinese Medicine. Therefore one can easily conclude that the dietetics is the most important and effective of all irrespective of Western and Chinese Medicine as far as diabetesis concerned. In Western Medicine, a diabetic is recommened to have the minimum calories necessary for life and not to have goods containing glucide beyond a certain quantity, while in Chinese medicine a diabetic is not allowed to have foods containing more than 10% of glucide. These two facts suggest that a diabetic should pay careful attention to foods containing lots of glucide. Finally a systematic cooperation between western and Chinese medicine will cure not only diabetes but also other disease more effectively than a traditionally unilateral method.

목적: 스포츠 건강 심리학 관점에서 건강에 영향을 미치는 심리적 요인과 건강 행동을 증진하는 방법을 탐구하기 위한 연구가 필요하다. 이 연구는 당뇨병 질환으로 인한 건강 관리와 유지를 위해 규칙적인 운동 참여가 필수적 인 당뇨병 환자들의 동기 유형을 파악하고, 동기 유형과 불안과의 관계를 규명했다. 방법: 당뇨병으로 진단받은 255명의 환자를 대상으로 동기 유형과 불안을 측정하고, 이들 변인 간의 관계를 분석했다. 부가적으로 불안 수준 과 성별에 따라 동기 유형의 차이를 확인했다. 결과: 연구결과, 확인규제는 불안에 부적 영향을 미쳤으며, 외적규 제는 불안에 정적 영향을 미쳤다. 차이검증에서도 통합규제와 확인규제가 높은 당뇨병 환자들은 불안 수준이 낮 은 것으로 밝혀졌으며, 외적규제가 높은 당뇨병 환자들은 불안 수준이 높은 것으로 나타났다. 이는 운동의 중요 성을 인식하는 동기를 갖고 규칙적인 운동에 참여할 때 불안이 낮아진다는 것을 의미한다. 반면에 외적 보상, 죄 책감이나 처벌을 피하려는 동기를 갖고 운동에 참여할 때는 불안이 높아진다는 것을 보여준다. 결론: 이러한 연 구결과는 당뇨병 환자들이 불안 수준을 낮추기 위해서는 개인이 지닌 동기 유형이 중요한 역할을 한다는 것을 보여준다. 특히, 통합규제와 확인규제를 지니고 운동에 참여하는 것이 중요하다는 것을 보여준다. 즉, 자신이 원 하고 운동의 중요성을 인식하고 자발적으로 운동에 참여하는 것이 심리적 건강에 이롭다는 것을 시사한다. 반대 로 가족이나 의사의 지시나 질책으로 운동에 참여하는 것은 심리적 건강에 해롭게 할 수 있다는 것을 예측할 수 있다. 결론적으로 이 연구는 당뇨병 환자들의 운동 상담 및 운동 프로그램을 구성하는 데 있어서 환자들의 동기 특성을 반영하고, 이를 계획하는데 유용한 기초 자료가 될 것이다.