This study evaluated the virucidal efficacy of a chlorine dioxide (ClO2) gas-generating fumigation disinfectant consisting of sodium chlorate solution (25% sodium chlorate) and reaction solution against avian influenza virus (AIV). After AIV suspensions had been deposited on stainless steel carriers, the 9 dried carriers were exposed to the fumigant (sodium chlorate solution: 8.5, 17, 34, 50, and 100 mL) in a 25-m³ test room for 2, 3, and 4 h, respectively. Thereafter, all carriers were submerged in a neutralizing solution (20% fetal bovine serum) to scrape off the surviving viruses, and the respective suspensions were diluted. Each diluent was inoculated into the allantoic membrane of five 10-day-old embryos. After incubation for 5 days at 37℃, AIV viability in the collected allantoic fluids was examined, and the egg infectious dose 50 (EID50) was calculated. When the carrier was exposed to ClO2 gas generated from reacting 34 mL of the fumigant for 3 h, the AIV titer reduced by more than 104.0 EID50/carrier compared to the control, which was exposed to the fumigant without inoculation of AIV suspension. In addition, the control was non-toxic to the embryos.

This study was conducted to reset the maximum residue limit (MRL) for didecyldimethylammonium chloride (DDAC) in broiler chickens. The disinfectant containing DDAC (10%, w/w) was diluted 160 times and evenly sprayed on the bodies of twenty-four broiler chickens at a rate of 15 mL per day per bird for 7 days. After the disinfectant treatment, tissue samples were collected from six broiler chickens at 0.25, 1, 3 and 5 days, respectively. Residual DDAC concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 2.0~10.0 μg/kg), recoveries (86.9~118.6%), and coefficient of variations (<19.98%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In all tissues except for liver, DDAC was detected more than LOQ at 5 days after the disinfectant treatment. In liver tissues, DDAC was detected more than LOQ at 3 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal period of DDAC in poultry tissues was established to 26 days. In conclusion, the developed analytical method is sensitive and reliable for detecting DDAC in poultry tissues. When DDAC disinfectant is sprayed on a poultry house in the presence of broiler chickens, it is necessary to keep the disinfectant from contacting the body of the livestock.

Despite the consumption of disinfectants have been increased by COVID-19 pandemic, the fate of the chemicals in aquatic food webs are still unclear. In order to understand the trophic transfer of the chemicals, the concentration of disinfectants including six benzalkonium chloride (BACs) and five didecyldimethylammonium chlorides (DDACs) were measured at the Geum (2020), Han (2021), and Yeongsan River (2021), before and after rainfall. The highest concentration of ΣBACs (mainly C12 and C14) and ΣDDACs (mainly C10 and C14) were observed in the Han River, followed by Yeongsan River, Geum River Estuary, and Gapcheon. After rainfalls, both concentration and detection frequency were decreased in all sites. Although the BAC and DDAC seems to be accumulated in organisms, they were bio-diluted rather than magnified in the aquatic food web with the biomagnification factor (BMF) of less than 1, trophic magnification slope (TMS) from - 0.236 to 0.001, and trophic magnification factor (TMF) from 0.85 to 1.01.

Aspergillus flavus (A. flavus) and Aspergillus fumigatus (A. fumigatus) are the main fungi that cause stonebrood in honey bees. Additionally, these fungi cause the declines of honey bee population and the economic loss in the beekeeping industry. In this study, the efficacy of a disinfectant, composed to chlorine dioxide (10%, w/v) and quaternary ammonium compound (12.5%, w/v), was evaluated against A. flavus and A. fumigatus. A fungicidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test fungi for 30 min at 4°C. The disinfectant and test fungi were diluted with low and high organic matter (OM) suspension according to treatment condition. On low OM condition, the fungicidal activity of the disinfectant against A. flavus and A. fumigatus was all 2.0 fold dilutions. On high OM condition, the fungicidal activity of the disinfectant against A. flavus and A. fumigatus was all 1.25 fold dilutions. The recommended dilution ratio of the disinfectant in low and high OM was 1.6 and 1.0 fold dilution, respectively. As the disinfectant possesses fungicidal efficacy against A. flavus and A. fumigatus, the disinfectant can be used to prevent the stonebrood in honey bees.

To check the linguistic features of the interviews from the humidifier disinfectant victims, the research compared the interviews with the modern spoken corpus and analyzed them in respect of vocabulary and grammar. The results are as follows. First, a common point was deduced by the lexical metaphor that the relationship between the informants and their companion animals, the victims of the case, is as close as a family member. The result is very meaningful, in that it is very rare that the materials of the lexical metaphor provide and predict the relationship between humans and companion animals. Second, the actuality of the semi-formal talk was well revealed by the grammatical feature, in terms of many fragmentary sentences and frequent uses of the ‘-haeyo’ style. However, certain types of conclusive endings such as ‘-keodeunyo’, ‘-jyo’, or ‘-jahnayo’, which are used by the speaker's assumption on the state of hearer's knowledge, were frequently used compared to the modern spoken corpus. The features of these conclusive endings assuming hearer's knowledge and ignorance show that the linguistic materials of informants implicate their intent to inform the society of the case and to get recognition from the society.

Highly pathogenic avian influenza virus (HPAIV) damages vital organs and tissues, frequently leading to death in birds, and causes serious economic losses in the poultry industry. In addition, HPAIV can infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Clean-Zone®, which contains citric acid, malic acid and phosphoric acid, against avian influenza virus (AIV, H9N2) was investigated. Virucidal efficacy was determined by examining the viability of AIV after contact with the disinfectant in the allantoic membrane of chicken embryos. The disinfectant and AIV were reacted under hard water (HW) and organic matter suspension (OM) condition. AIV was inactivated with 200- and 50-fold dilutions of the disinfectant under HW and OM conditions, respectively. As the disinfectant, Clean-Zone®, has a virucidal efficacy against AIV, it can be used to prevent the spread of animal viral diseases.

The objective of this study was to determine the efficacy of ozone in sanitizing water experimentally inoculated with the gram-positive food-poisoning bacterium Staphylococcus aureus. The bactericidal effect was measured after experimentally inoculated solutions were exposed to 0, 0.5, and 1.0 ppm ozone at several time points and different temperatures, in the presence of varying concentrations of different organic matter, namely, fetal bovine serum (FBS) or cattle liver. Results revealed inhibition of the bactericidal effect in the presence of the lowest percentage of FBS, but a lower extent of the inhibition occurred when liver was used as the organic matter. It was also apparent that a higher temperature and shorter ozone exposure time had led to a more reduced bactericidal efficacy than that under a lower temperature and longer ozone exposure. This study provides insight into the potential use of ozonated water as an effective and safe disinfectant in an abattoir setting.

살모넬라증은 가축에 심각한 피해를 유발하는 질병으로, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 복합 인산염을 주성분으로 하는 분말 소독제의 Salmonella Typhimuriums에 대한 살균효과 시험을 수행하였다. 배지희석법을 이용한 살균효력시험은 4℃에서 30분 동안 시험 세균을 희석 소독제에 노출시켜 소독제의 가장 효과적인 낮은 희석배수를 결정하는 시험이다. 본 분말 소독제와 시험 세균을 처리조건에 따라 경수와 유기물로 희석하여 반응을 시켰다. 유기물 조건에서, Salmonella Typhimurium에 대한 소독제의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 사료된다. 분말 소독제는 Salmonella Typhimurium과 같은 병원체에 대해 살균효과를 가지므로, 살모넬라증과 같은 세균성 질병의 확산을 제어하는데 효과적으로 이용될 수 있을 것으로 사료된다.

Disinfectant-filled footbaths are the most commonly used infection control measures on livestock farms. The purpose of this study was to evaluate the efficacy of six commercial disinfectants, as used in footbaths on farms, for reducing Salmonella Typhimurium contamination of footwear. When used at the recommended concentrations, most disinfectants showed over 91% reduction efficacy in absence of organic matter. The greatest reductions were seen with oxidizing agents including potassium peroxymonosulfate-based products and sodium dichloroisocyanurate. These results suggest that disinfectant footbaths could be useful for reducing bacteria; however, footbaths should not be relied upon as the only method of controlling infectious pathogens. General good hygiene practice, for example cleanliness of boots, should be emphasized.

The coronavirus porcine epidemic diarrhea virus (PEDV) infects the cells lining the small intestine of a pig and, causes porcine epidemic diarrhea (PED). Owing to its highly infectious nature, PEDV has a substantial economic burden, which results in significant morbidity and mortality in piglets. In this study, the virucidal efficacy of a powder disinfectant containing a phosphate compound against PEDV was investigated. Virucidal efficacy was assessed as the infectivity of PEDV toward Vero cells after exposure of the virus to the disinfectant. PEDV was exposed to the disinfectant in the presence of either hard water (HW) or an organic matter suspension (OM). In the HW condition, PEDV was inactivated by 4-fold dilution of the disinfectant. In the presence of OM, the disinfectant showed virucidal activity with a 2-fold dilution. As the disinfectant possessed virucidal activity against PEDV, it should be an effective reagent for limiting the spread of animal viral diseases.

살모넬라증과 부루셀라증은 가축 및 사람에 심각한 피해를 유발하는 질병으로, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 자몽종자추출물, 구연산, 사과산 그리고 염화벤잘코늄을 주성분으로 하는 스프레이형 소독제의 Salmonella Typhimurium과 Brucella ovis에 대한 효력시험을 수행하였다. 살균효력시험은 배지희석법에 따라 수행하였으며, 스프레이형 소독제와 시험 세균들을 처리조건에 따라 경수와 유기물로 희석하여 반응을 시켰다. 유기물 조건에서, Salmonella Typhimurium과 Brucella ovis에 대한 스프레이형 소독제의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 사료된다. 스프레이형 소독제는 Salmonella Typhimurium과 Brucella ovis와 같은 인수공통전염병 유발 병원균들에 대해 살균효과를 나타내어, 살모넬라증과 브루셀라증과 같은 세균성 질병의 확산을 제어하는데 효과적으로 이용될 수 있을 것으로 사료된다.

본 연구는 S. Typhimurium을 대상으로 paraformaldehyde 35%를 함유한 훈증소독제의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, S. Typhimurium의 현탁액 균수는 모두 3.9 × 108CFU/mL이었으며, 훈증소독제에 노출시킨 모든 담체의 균수는 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조담체로부터 회복된 S. Typhimurium 균수는 모두 3.3 × 106 CFU/mL이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 S. Typhimurium의 감소 균수는 5.22 log CFU/mL로 나타났다. 이상의 결과로부터, paraformaldehyde를 주성분으로 하는 훈증소독제는 S. Typhimurium에 대해 효과적인 살균력을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

Disinfectant/oxidation process is a crucial process in water treatment for supplying safe drinking water. Chlorination is still widely used for water treatment area due to its effectiveness on microbial inactivation and economic feasibility. Recently, disinfection concern in marine environment is increasing, for example, movement of hazardous marine organism due to ballast water, marine environmental degradation due to power plant cooling water discharge, and increase of the amount of disinfectant in the offshore plant. It is needed to conduct the assessment of disinfectant behavior and the development of disinfectant prediction model in seawater. The appropriate prediction model for disinfectant behavior is not yet provided. The objective of the study is to develop chlorine decay model in seawater. Various model types were applied to develop the seawater chlorine decay model, such as first order decay model, EPA model, and two-phase model. The model simulation indicated that chlorine decay in seawater is influenced by both organic and inorganic matter in seawater. While inorganic matter has a negative correlation with the chlorine decay, organic matter has a positive correlation with the chlorine decay.

본 연구는 겨울철에 상대적으로 낮은 기온으로 인하여 소독효과가 저하되는 기존의 차량소독시스템을 개선함으로써 우리나라의 겨울철에도 차량소독장치의 분무노즐로부터 분사되는 소독약액이 동결되지 않 고 분무 시 소독약액의 온도가 유효온도인 15∼20℃ 범위 내에서 유지되도록 하여 농장출입차량에 대 한 소독효과를 높여 가축전염병에 대한 차단방역이 확실히 이뤄지도록 하기 위해 수행되었다. 이를 위 해 분사 후 소독약액의 온도가 유효온도 범위 내에서 유지되도록 하기 위한 소독약액탱크 내 소독약액 의 적정온도 및 펌프와 분무노즐 사이에 있는 호스와 분무노즐의 동결을 방지하기 위한 열선의 용량을 결정했다. 기온이 –25℃인 상황을 가정하여 실험한 결과, 분사되는 소독약액의 온도를 유효온도의 하한 치인 15℃ 이상으로 유지하기 위한 소독약액 탱크 내 소독약액의 온도는 약 30℃이었으며, 호스와 분무 노즐의 동결을 방지하기 위한 열선의 용량은 26W m-1 이상이었다.

Porcine epidemic diarrhea virus (PEDV) causes porcine epidemic diarrhea (PED) and a considerable economic loss in the swine industry. In this study, the virucidal efficacy of a disinfectant composed of citric acid, benzalkonium chloride and phosphoric acid against PEDV was investigated. Virucidal efficacy was assessed as the infectivity of PEDV toward Vero cells after exposure of the virus to the disinfectant. PEDV was exposed to the disinfectant in the presence of either hard water (HW) or an organic matter suspension (OM). In HW condition, PEDV was inactivated by 600-fold dilutions of the disinfectant. In the presence of OM, the disinfectant showed virucidal activity after a 200-fold dilution. As the disinfectant possesses virucidal activity against PEDV, it should be an effective reagent to use to limit the spread of animal viral diseases.

Clostridium perfringens (C. perfringens) 와 Mycobacterium fortuitum (M. fortuitum)은 동물과 사람에서 심각한 질병과 관련이 있는 세균들로 알려져 있다. 본 연구에서는, povidoneiodine을 주성분으로 하는 소독제의 살균효과를 C. perfringes와 M. fortuitum을 대상으로 평가하였다. 소독제의 살균효과는 배지희석법을 이용하여, 대상 세균들을 4℃에서 소독제에 30분 동안 노출시킨 다음, 가장 낮은 소독제의 살균 희석배수를 결정하였다. 소독제는 경수와 유기물로 희석하였으며, 경수 조건에서, C. perfringes와 M. fortuitum에 대해 효과적인 소독제 희석배수는 각각 50과 80배이었다. 유기물 조건에서는, C. perfringes와 M. fortuitum에 대해 효과적인 소독제 희석배수는 모두 15배로 나타났다. 이상의 결과로부터, povidone-iodine을 주성분으로 하는 소독제는 C. perfringes와 M. fortuitum에 대해 살균효과를 갖는 것으로 확인되었으며, C. perfringes와 M. fortuitum에 의한 질병의 확산을 방지하기 위해 사용될 수 있을 것으로 사료된다.

본 연구는 P. aeruginosa와 E. hirae을 대상으로 orthophenylphenol 20%를 함유한 훈증소독제, Fumagari OPP®의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, P. aeruginosa와 E. hirae의 현탁액 균수는 각각 2.8 × 108 와 3.6 × 108CFU/mL이었으며, 모든 훈증소독제에 노출시킨 담체의 균수는 모두 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조 담체로부터 회복된 P. aeruginosa와 E. hirae 균수는 각각 2.9 × 106와 2.7 × 106CFU/mL이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 P. aeruginosa 와 E. hirae의 감소 균수는 각각 6.46와 5.19 logCFU/mL 로 나타났다. 이상의 결과로부터, 훈증소독제, Fumagari OPP®는 P. aeruginosa와 E. hirae에 대해 효과적인 살균력 을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

This test was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 20% ortho-phenylphenol against Pseudomonas aeruginosa (P. aeruginosa) and Enterococcus hirae (E. hirae). In preliminary tests, P. aeruginosa and E. hirae working culture suspension number (N value) were 2.8 × 108 and 4.0 × 108CFU/mL, respectively. And all the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of P. aeruginosa and E. hirae recovered on the control-carriers (T value) was 2.8 × 108 and 3.4 × 106 CFU/mL, respectively. In the bactericidal effect of the fumigant, the reduction number of 2.8 × 108 (d value) was 6.46 and 5.19 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results from this study, the fumigation disinfectant containing 20% ortho-phenylphenol has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with the pathogenic bacteria.

Vibrio spp. and Streptococcus spp. have caused a considerable disease of farmed fish and economic loss in fish farming and seafood industry. In this study, the efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite was evaluated against V. anguillarum and S. iniae. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4oC. An aquatic disinfectant tablet and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. V. anguillarum on the DW, HW and OM condition was completely inactivated with 16,000 15,000 and 13,000 fold dilutions of the disinfectant, respectively. On the DW, HW and OM condition, S. iniae was absolutely inactivated with 17,000 16,000 and 14,000 fold dilutions of the disinfectant, respectively. As an aquatic disinfectant tablet possesses bactericidal efficacy against fish pathogenic bacteria such as V. anguillarum and S. iniae this disinfectant solution can be used to control the spread of fish infective bacterial diseases.

In this study, the veridical efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite against red sea bream iridovirus (RBIV). A veridical efficacy was determined with the viability of RBIV contacted with the disinfectant in viral stock cultured in fat head minnow cell line. An aquatic disinfectant tablet and RBIV were reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW and HW condition, RBIV was inactivated with 25,000 fold dilutions of an aquatic disinfectant tablet. With the investigation of the antiviral effect of the disinfectant on OM condition, RBIV was inactivated on 22,000 fold dilutions of an aquatic disinfectant tablet. As an aquatic disinfectant tablet possesses veridical efficacy against RBIV, the disinfectant solution can be used to limit the spread of cultured marine fish viral disease.