Comparative effect of oyster mushrooms on plasma, fecal lipid profiles, liver and kidney functions were evaluated in hyper and normocholesterolemic rats. The feeding of hypercholesterolemic rats with 5% powder of fruiting bodies of oyster mushrooms i.e., Pleurotus ostreatus, P. sajor-caju and P. florida reduced the plasma total cholesterol level by 37%, 21% and 16%, respectively and triglyceride level by 45%, 24% and 14%, respectively. LDL/HDL ratio decreased by 64%, 45% and 41% for P. sajor-caju, P. ostreatus and P. florida fed rats, respectively. Mushroom feeding also reduced body weight in hypercholesterolemic rats. However, it had no adverse effect on plasma bilirubin, creatinin and urea nitrogen level. Mushroom feeding also increased the total lipid and cholesterol excretion through the feces. The present study reveals that feeding of 5% oyster mushroom powder do not have detrimental effects on the liver and kidneys rather may provide health benefits for the cardiovascular-related complication by decreasing the atherogenic lipid profiles.

The bioactive effects of ethanol extracts from fly maggot (ME) on reduction of plasma lipids levels in rats fed high-fat diets (Expt. Ⅰ), and on liver function recovery of hepatotoxicity rats by intraperitoneal injection of carbon tetrachloride (CCl4) or by orally administration of alcohol (Expt. II) were investigated. In expt. I, twenty seven, male rat SDS(sprague dawley strain) were randomly assigned to three treated groups, including normal control group, HF (group with high fat diets which have no extracts) and HFE (HF plus orally administered doses of ME extract at 5.0 mg/100g of body weight). In expt. II, forty five, male rats (SDS) were randomly assigned to each of the five groups: T1 (control), T2 (intraperitoneal injection of CCl4), T3 (intraperitoneal injection of CCl4 after orally administered with ME), T4 (orally administered with combination of ME and alcohol), T5 (orally administration of ME after orally administered with alcohol). There were significant decreases in plasma (TAG), (TC), (LDL-C) in the HFE group with orally administered doses of ME at 5.0 mg/100g of body weight, respectively, however, the (HDL-C) were significantly increased in HFE group as compared to HF group with high fat diets which have no extracts (p〈0.05). The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transferse(γ-GTP) and bilirubin were highest in T2 or T3, and high in order T4 or T5, and lowest in T1 except for bilirubin which has same with T4, T5 (p〈0.05). The high recovery of liver damage by CCl4 from the light microscopic appearance was observed in rats (T3) with extracts, and also high in T4 than T5 by orally administrated with alcohol. In conclusion, the ethanol extracts from fly maggot may have a bioactive effects to prevent for human lipids disorder and alcoholic disease.

The biological effects of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) including benzo[a]pyrene (BaP), dibenzo[a,h]anthracene (DBA), benzo[a]anthracene (BaA), benzo[b] fluoranthene (BbF), benzo[k]fluoranthene (BkF), and indeno[1,2,3-c, d]pyrene (

Objective : Liver cancer is common cancer generating 11.3% incidence in Korea. But Oriental medical doctor (OMD) often can't measure therapy on liver cancer positively because that the prognosis on it is not good. It is impossible the results on all of diseases not only liver cancer always to have to be good. Methods : It is studied the literature referencing liver cancer, experimental articles to insist the effects of Oriental medicine planet to liver cancer and the clinical cases on liver cancer to be treated as Oriental medicine therapy to have effects. Results : it is required to find out the causes of diseases and therapy method to conquest the diseases for the medical therapy. But because the results on medical therapy are not always good, it is necessary for the doctor who want to treat the diseases like liver cancer having the prognosis to be death to protect himself, like as the legal system, a medical policy. And consequently, this protecting legal system lead the medical therapy principle or method on some special diseases to therapy completion on it. As a results of policy, medical therapy have to be developed. Recently, there have been many other development in oriental medical therapy. This development can be grouped as two type. One type is clinical part and the other is experimental research part. Specially the experimental research and clinical research on live cancer in oriental medical therapy have been developed. So the present day is the time to make medical policy on liver cancer for OMD to measure oriental medical therapy and develop oriental medical theory. Conclusions : As a above results, we propose that the name of liver cancer have to use in Korean medicine security clinical name (한방의료보험상병명) to make oriental medical policy.

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

In this study, 3,301 domestic and foreign patents, established from 1980 to June, 2007, were reviewed in order to examine the technological trends in treating or preventing liver disease. Primary focus was placed on countries such as Korea, Japan, and the United States - the leading nations with regard to liver function research. This paper explores the systematic search that utilized a research-focused database and research analysts. Since the year 2000, Korea has demonstrated increased developments in the area of diet and liver disease, as shown by an increase in relevant patent caseloads and submissions especially for medicinal preparations containing compounds or reaction products with undetermined constitutions. In addition, there was up to a 29% increase in themarket share and the frequency of patent submissionshad increased. Japan has shown a similar trend to Korea with an increase in research, but has focused more on medical preparations containing active organic ingredients. In the United States, an increase in the number of patents was shown after the year 2000.

본 연구에서는 내분비계 장애물질으로 알려져 있고, 플라스틱 제품의 가소제로 사용되고 있는 di-(2-ethylhexyl) phthalate (DEHP)가 흰쥐의 간세포 미세구조와 간조직내 metallothionein (MT)의 발현 양상에 미치는 영향을 조사하였다. DEHP는 흰쥐 간세포의 미세구조와 MT 발현에 영향을 주었다. 실험군의 경우 조면소포체가 발달하고, 미토콘드리아가 증가하며 리소솜 혹은 퍼옥시좀들이 증가하는 경향을 나타냈다. 한편, MT

본 연구에서는 SV40 Tag을 마우스 albumin 유전자의 promoter/enhancer 조절 하에 발현하도록 설계된 재조합 유전자를 마우스 1세포기 수정란에 미세 주입하여 형질 전환 마우스를 제작하고 이들의 인체 간암 모델로써의 적합성을 조사하였다. 형질 전환이 확인된 총 11개체의 founder 생쥐들 중 4개체가 간암을 일으켰고, 두 개체는 신장암을, 한 개체는 피부 및 뇌에서 종양을 각각 일으켰다. 이들로부터 외래 유전자를 계대 유전하는 3가계를 얻었다(#1-2, #1-6, #1-11). 이들 가계의 자손들에서 8주령(#1-2, #1-6) 혹은 10주령(#1-11) 시부터 간암이 반복적으로 발생되었으며, #1-11 founder 개체에서 폐로 암세포가 전이된 것 외에는 다른 조직에서의 형태학적 변이가 발견되지 않았다. 간암 발생은 조직학적 변화에 따라 3단계로 나눌 수 있었다. 즉, 출생에서 3주령까지는 간세포의 과량증식을 보이나 세포핵의 이상은 관찰되지 않았으며, 4주령부터 7주령(#1-2, #1-6) 혹은 9주령(#1-11)까지는 diffuse liver cell dysplasia를 나타내지만 tumor nodule은 발견되지 않았고, 그 이후에는 liver dysplasia를 배경으로 간암이 발생하였다. 본 연구에서 작출한 간암 모델 마우스는 인체 간암과 일부 유사한 소견을 보였는 바 인체 간암 기전 연구를 위한 유용한 동물 모델로 이용할 수 있을 것으로 생각된다.

The effect of treatment with Citrus junos fractions (citron 3W, citron 3H, citron 4W and citron 4H) upon rat hepatocytes exposed to alcohol was investigated. We compared the serum biochemistry of rats administered both alcohol and Citrus junos fractions t

In order to investigate whether or not the alcohol-treated rat liver cells can be protected by Korean Citrus junos and medicinal herbs, We compared the serum biochemistry of rats administered both alcohol and the complex of Korean Citrus junos and medicin