The house fly, Musca domestica L. is a serious cosmopolitan pest in livestock production systems. Poultry farmers employ various techniques to control them but rely heavily on the use of chemicals which has the serious drawback of the target pest becoming resistant. We have selected two native natural enemies in Gyeonggi area where selected for the biological control of the house fly. Carcinops pumilio (Erichson) (Coleoptera: Histeridae), commonly found in poultry manure, is an important predator of house fly eggs and larvae. The substitute food source was developed for the mass rearing of C. pumilio. Spalangia nigroaenea (Hymenoptera: Pteromalidae) not only parasitized the host to produce its progeny but also killed host pupae by feeding. The parasitoid induced mortality had an effect on the parasitoid-to-host density relationships.
A novel insect-infecting positive sense single-stranded RNA virus, Riptortus pedestris virus-1 (RiPV-1), was found in the Riptortus pedestris transcriptome data by de novo assembly and further confirmed by RACE method. The genome of RiPV-1 consists of 10,554 nucleotides (nt) excluding the poly(A) tail and contains a single large open reading frame (ORF) of 10,371 nt encoding a 3,456 aa polyprotein and flanked by 71 and 112 nt 5' and 3' noncoding regions, respectively. RiPV-1 genome contains the consensus genome organization of picorna-like RNA helicase, cysteine protease, and RNA-dependent RNA polymerase (RdRp) array in that order from the 5' to the 3' end. From the phylogenetic analysis, RiPV-1 was clustered with unassigned insect RNA viruses, APV and KFV, which suggests that these three insect picorna-like viruses might constitute a novel group of insect-infecting RNA viruses. Tissue tropism analysis revealed that RiPV-1 was relatively abundant in the thorax, abdomen, midgut and fat body. Interestingly, RiPV-1 replication was enhanced by Beauveria bassiana JEF-007 infection that was quantified using qRT-PCR. This study identified a novel insect-infecting virus and provided further insight into the relationship between virus, fungus and host.
A seasonal outlook for crop insect pests is most valuable when it provides accurate information for timely management decisions. In this study, we investigated the probable relationship between climatic phenomena and pest infestations in Korea using two statistical methods. Brown planthopper was selected because of its migration characteristics, which fits well with the concept of our statistical modelling – utilizing a long-term, multi-regional influence of selected climatic phenomena to predict a dominant biological event at certain time and place. The moving window regression (MWR) model showed high correlation between the national infestation trends of brown planthopper and some tempo-spatial climatic variables near its sequential migration path, while the climate index regression (CIR) model resulted in a relatively low correlation compared to the MWR model. Overall, the statistical models developed in this study showed a promising predictability for rice brown planthopper infestation in Korea, although the dynamical relationships between the infestation and selected climatic phenomena need to be further elucidated.
A subtropical insect species, Maruca vitrata, is invasive to temperate zones. Supercooling capacity of M. vitrata was confirmed in all developmental stages, in which egg exhibited the lowest supercooling point (SCP) at –22.5oC. However, low temperature lethality was observed at much higher temperatures than SCPs in all developmental stages. In addition, this nonfreezing injury increased with incubation time at rearing condition. Uncontrolled phenoloxidase activation appeared to cause the nonfreezing injury. Preexposure to a cool temperature before lethal low temperature significantly increased survival of M. vitrata. This cold hardening accompanied with increase of glycerol content in hemolymph. Transcriptome of M. vitrata under cond-hardening was assessed.
Seasonal abundance and distribution of mosquitoes and chigger mites are important for human populations. However, factors affecting the abundance and distribution of vector species are not well - understood. Furthermore, global climate change makes it difficult to predict the population dynamics of vector species. Therefore, it is important to monitor this vector species over a long period of time. In 2015, mosquitoes were collected from three potential mosquito habitats using black light trap and BG-Sentinel trap in Gyeongi-do in April through October. Also, we collected chigger mite using sherman live traps in Cheorwon, Gangwon-do in April, September and October. From the survey, we collected a total of 11,649 mosquitoes in three potential mosquito habitats, and a total of 3,656 chigger mites from 54 wild rodents. In the samples, dominant mosquito species were Aedes vexans nipponii (Species Ratio 70%), Culex pipens pallens (SR 9.8%) in Gyeonggi area and dominant chigger mite species was Leptotrombidium pallidium (SR 59%) in Cheorwon. The results of this study can serve as provide fundamental basis to develop effective management strategy under the global climate change environment.
채소류에 발생하는 주요 바이러스는 WMV, CMV, TSWV, TYLCV 등으로 위축, 얼룩무늬, 기형 등과 같은 증상을 일으켜 품질과 수량에 큰 피해를 초래하고 있다. 이 들 바이러스의 전염은 주로 곤충에 의해 매개되는데 WMV, CMV는 진딧물에 의해, TSWV는 총채벌레, TYLCV는 담배가루이가 매개하는 것으로 보고되어 있으며 방제를 위해서는 매개충의 밀도를 줄이는 것이 중요하다고 할 수 있다. 경북 칠곡군 등 3개 지역의 비닐하우스 주변에 자생하는 잡초를 초종별로 채취한 후 진딧물과 총채벌레의 월동밀도를 조사하였고 채집된 매개충과 잡초는 모두 핵산을 추출하여 바이러스 보독여부를 조사하였다. 하우스 주변의 주요 월동잡초는 뚝새풀, 엉겅퀴, 무, 고들빼기, 유채, 바랭이, 개쑥갓 등 이었고 이들 잡초에서 월동하는 해충은 복숭아혹진딧물, 꽃노랑총채벌레, 대만총채벌레 등 이었다. 이 중 진딧물과 총채벌레에서 바이러스 보독이 확인되었다. 월동해충 밀도는 고들빼기, 유채 등의 잡초에서 진딧물은 1~2마리/주 정도였으며, 무, 유채 등의 잡초에서 총채벌레는 2~3마리/주 정도였다. 이상의 결과로 보아 채소류 재배지의 주변 잡초 등에서 월동한 바이러스 보독충이 초기 바이러스 발생의 주요 매개원일 것으로 판단되며 작물 재배 전 월동 보독충의 밀도를 낮추는 것이 채소류 주요 바이러스의 방제를 위한 주요한 수단일 것이다.
Pheromone biosynthesis-activating neuropeptide (PBAN), produced in subesophageal ganglion, is known to stimulate pheromone biosynthesis in Plutella xylostella. The pheromone production is more active in the scotophase than in the photophase, which suggests that there may be changes of gene expression in the pheromone glands. To analyze gene expression involving in pheromone biosynthesis for 24 hrs, we performed transcriptomes of pheromone glands which were isolated at every 4 h. Eleven pheromone biosynthesis-related genes, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), acyl-CoA dehydrogenase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, Δ9 desaturase, Δ11 desaturase, fatty acid reductase (FAR), alcohol oxidase, aldehyde oxidase, and aldehyde reductase were identified. Among these genes, ACC, FAS and chain shortening enzymes involving in early stage of pheromone biosynthesis exhibited their highest expression between AM9 and PM5. Desaturases, Δ9 and Δ11, showed the peak of expression at PM1 and AM5 or PM5, respectively. Interestingly, FAR expression was the highest at AM1, active reproductive period. These results suggest that genes involving in pheromone biosynthesis can be sequentially regulated for their biological roles.
Pheromone biosynthesis activating neuropeptide (PBAN) produced in the subesophageal ganglion is known to stimulate pheromone production in the pheromone gland. A cDNA isolated from female adult heads of Maruca vitrata encodes 197 amino acids including PBAN, designated as Mvi-PBAN, and four other neuropeptides (NPs): diapause hormone (DH) homologue, α-NP, β-NP and γ-NP. All of the peptides are amidated in their C-termini and shared a conserved motif, FXPR(or K)L-NH2 structure. Mvi-PBAN consists of 35 amino acids as previously reported (Chang and Ramasamy, 2014). RT-PCR analysis revealed that Mvi-PBAN cDNA was expressed in all examined body parts. Nucleotide sequence analysis of RT-PCR products indicated the Mvi-PBAN sequence was identical in all examined body parts of both sexes. These results suggest that Mvi-PBAN expression is maintained in examined stages or tissues.
This taxonomic study on the genus Scutellathous is only recognized a species. S. porrecticollis (Lewis, 1894) in Korea. The previous record of Athous (=Medakathous) jactatus (Lewis, 1984) is revealed as the misientification of S. porrecticollis. We also describe and illustrate Subscutellathous koreanicus gen. and sp. nov. from Korea. This new genus si compared to the most closed genus Scutellathous by the basis of diagnostic characters in generic level.
국내에 있는 목조문화재를 가해하는 종은 구조적인 문제를 야기 시키는 흰개미 목에 흰개미, 외관에 천공을 내어 가해하는 벌목에 속한 나나니벌이나, 외관에 갱도를 내어 가해하는 딱정벌레 목에 속한 빗살수염벌레 및 넓적나무좀 등이 알려져 있다. 조사 방법은 충청 남·북도 내에 있는 24곳의 목조문화재를 대상으로 하였다. 흰개미 탐지견을 이용하여 흰개미 탐지를 한 후 탐지된 곳을 중점적으로 조사 하고 탐지가 되지 않은 곳도 육안 관찰을 하여 흰개미 가해흔이 있는지를 면밀히 조사하고 흰개미가 아닌 다른 충해도 있는지를 조사하여 기록하고, 건물 방위 및 GPS 위치, 온․습도, 함수율, 난방여부 등을 조사하여 해충이 가해하기 쉬운 환경 인자를 조사하여 기록하였다. 이 후 주변부 흰개미가 서식할 수 있는 그루터기나 고목 등을 조사하고 흰개미가 발견되면 즉시 샘플링하고 제거하였다.
Metam-sodium has been generally applied to control Pinus koraiensis, P. densiflora and P. thunbergii infected by pine wilt disease in the forest field of Korea. We determined the atmospheric concentration of nematocidal and insecticidal gaseous methyl isothiocyanate (MITC) and more volatile and toxic gaseous methyl isocyanate (MIC) in the metam-sodium fumigated fields depending on the distance from metam-sodium treated site, time and season by OSHA No. 52 method. Determined atmospheric MIC was below acute exposure limit (50 ㎍/㎥) and chronic exposure limit (1.0 ㎍/㎥) of EPA regulation in all detection point. In summer, the maximum of MITC and MIC was observed to 105.2 ㎍/㎥ in the 1st day and 0.41 ㎍/㎥ in the 4th day after metam-sodium treatment, respectively. On the other hand, in winter, the maximum of MITC and MIC was observed to 4.6 ㎍/㎥ in the 4th day and 0.52 ㎍/㎥ in the 5th day after metam-sodium treatment, respectively. Atmospheric concentrations of MITC and MIC were higher the closer to the metam-sodium treated site, but decreased below LOQ with distance (~ 50 m). This result showed that the reaction rate of metam-sodium to MITC is more affected by temperature and the translation rate of MITC to MIC is generally very low, regardless of season.
소나무재선충병 피해목을 제거하는 방법 중 매개충이 서식하는 벌채목 대상으로 하는 훈증방제법이 있다. 그러나 훈증방법 은 피복재가 훼손되면 재산란의 우려가 있다. 또한 숲가꾸기 산물을 임내 존치했을 때도 매개충(솔수염하늘소)의 서식처로 활용될 수 있다. 본 실험은 훈증목과 숲가꾸기 산물에 대한 매개충의 산란여부와 진주, 사천, 거제의 4개소 109개 훈증무더기에 대해 훈증 피복재의 훼손여부를 알아보았다. 2010년~2014년까지 동일공간에 배치한 훈증목에 매개충을 방사한 결과, 2010년~2013년 훈증목에서는 유충이 없었지만 2014년 훈증목에서는 솔수염하늘소의 유충이 발견되었다. 그러나 2013년과 2014년 훈증목을 각각 따로 사육상에 배치 후 산란을 유도했을 때 2013년 처리구에서 유충 1마리가 발견되었다. 소나무재선충 병 피해지 3지역 4개소에서 훈증무더기를 109개 대상으로 훈증 피복재의 찢어짐, 구멍 등을 조사한 결과 평균 14.7 %의 훼손율을 보였지만 2015년 훈증무더기 피복재는 전혀 훼손되지 않았다. 또한 2012년부터 2014년까지 리기다소나무(Pinus rigida)를 대상으로 숲가꾸기를 실시한 지역에서 존치된 벌채목에서 하늘소류의 침입공과 탈출공이 다수 발견되었다. 숲가꾸 기 산물을 수집 후 야외 사육상에 배치 후 매개충의 산란을 유도한 결과 2012~2014년 산물에서는 유충이나 유충 침입공을 발견할 수 없었다.
It is difficult to identification between Bursaphelenchus spp. and Pine Wood Nematode (PWN) by morphological characteristics without expertise about nematode taxonomy. Furthermore, Baermann funnel method, which is nematode extraction method from wood chips or soil, requires at least 24 hours to extract nematode that is unsuitable to rapid diagnose the Pine Wilt Disease (PWD). For these reasons, the aim of this experiment is not only to improve accuracy of a PCR based method but also to reduce total experiment time for detection Bursaphelenchus spp. and PWN in the wood chips of PWD infected pine tree. In this experiment, we had been employed two PCR primer sets, which were originated from PWN specific Internal Transcribed Spacer (ITS) sequence region and Bursaphenchus spp. universal mitochondrial Cytocrome Oxidase subunit I (mtCOI) sequence region in order to discrimination between Bursaphelenchus spp. and PWN at the same PCR reaction. This experimental procedure was able to reduce experiment time and cost as well as to improve accuracy of detection than previous PCR based detecting method by not using Baermann funnel method and commercial genomic DNA extraction kit but using direct pine wood chips lysis method.
Species identification, the process of finding the taxon to which a specimen belongs, is a difficult and frustrating task for most biologists but is absolutely fundamental to most biological research. However, proper species identification requires extensive knowledge on the particular taxon, thus can be problematic and confusing for non-professionals. To increase the accuracy of identification, computer-based interactive identification key has been widely used. In order to improve the identification process and promote effective utilization of Korean species, Korean Indigenous Interactive Key System (KIIS) was developed. Moreover, image-based online identification system was developed to broaden the range of users from non-experts to professionals. As the use of mobile device became significant in people’s lives, we further developed mobile web-based identification system.
Alfalfa weevil (Hypera postica Gyllenhal) belongs to the family Curculionidae, Order Coleoptera and it is one of invasive alien species in Korea. In Korea environment, this species was found in 2000 first time through the Korean quarantine inspections. Although various researches have been studied in the Europe, US, China and Japan, only few research has been performed in Korea. In this study, the life cycle, morphology and the host plants of Alfalfa weevil were investigated in the natural environment. Nine plants of total 28 host plant species known as the host plants were characterized as their wild host plants in Korea (6 and 3 of 9 were naturalized and native plants in Korea, respectively). The geographical distribution was very wide from south (Jeju) to north (Yeoncheon) area of the peninsula and the favorite habitats were park, cultivated and grass land. This species has been found easily anywhere because it takes common leguminous plants such as clover, chinese milk vetch and alfalfa. These results suggest that Alfalfa Weevil can be one of the most-damaging insect pests of leguminous crops when it adapts to Korean climate.
In every summer, cicadas emerge and become numerically and ecologically dominant in Korean penninsula. Especially, cicada emergence is affected by the environmental factors. In order to evaluate the effect of environmental factors in cicada species, we analyzed the temporal changes in cicada exuviae based on meteorological and non-meteorological factors such as artificial light intensity and habitat characters on urban park area surrounded by residential houses. Combined multivariate analyses with a cluster analysis and a principal component analysis (PCA) were conducted. Samples were classified into 3 different cluster based on differences of meteorological factors such as temperature and humidity. Moreover, Random Forest model (RF) showed a high predictability of daily mean temperature on species peak abundance. These results provide a strong evidence that meteorological factors have significant effects on cicada emergences. Regarding the non-meteorological factors, we found no relationship in cicada emergence.
A cDNA (814 bp) encoding ornithine decarboxylase (ODC) antizyme (Az) was cloned from the Tobacco cutworm, Spodoptera litura. The open reading frame (ORF) showed an internal stop codon (TGA) on bases 223-224-225, encoding 270 amino acids except for the 223-T. A modified ODC Az cDNA (Slit ODC AzM) by point mutation for removal of the 223-T was introduced into a yeast (Saccharomyces cerevisiae) strain expressing a cloned ODC from the Tobacco cutworm. The transformed strain with ODC AzM exhibited a remarkable inhibition on ODC action, showing 56 % lowered cellular proliferation rate and the decreased cellular polyamine levels by 40.1 % (putrescine), 64.1 % (spermidine) and 58.6 % (spermine) at 48 hrs of cultivation in comparison with the original ODC Az cDNA. These results suggest that the modified ODC Az is expressed regardless of the frame-shifting and the expressed functional protein effectively inhibits the ODC activity.
In the present study we report a newly recognized crambid species, Sinibotys butleri (South, 1901). Previously, two species, S. evenoralis and S. obliquilinealis, were known from Korea, but now they are belonged to a genus Torulisquama Zhang, 2010, that was newly erected in a recent study. Sinibotys differs from Torulisquama by having: forewing 36-47.5 mm in length and without unscaled areas; male with transtilla covered with setae and editum bearing dense setae; female with ductus bursae relatively broad and short; corpus bursae and signum much larger. We update taxonomic position of these three species accordingly and compare Sinibotys and Torulisquama morphologically. A Key to species, description of adult, male and female genitalia structures are provided with available information such as collection locality, host plant and distribution range for the species.
본 조사를 통하여 2015년 6월부터 8월까지 태안군에서 13개 지역을 선정하여 곤충상을 조사 하였다. 주요조사방법으로 임도와 등산로, 생태탐방로를 따라 포충망을 이용한 채어잡기와 쓸어잡기 방법으로 주간 채집을 실시하였고, UV light trap을 이용한 야간 채집을 수행하였다. 채집된 육상곤충은 총 12목 77과 491종 3657개체가 확인되었고, 이 중 고유종으로 줄우단풍뎅이 (Gastroserica herzi Heyden, 1887, 딱정벌레목 : 검정풍뎅이과), 우리벼밤나방 (Sesamia pseudoturpis Kononenko et Ahn, 1998, 나비목 : 밤나방과), 제일줄나비 (Limenitis helmanni Lederer, 1853, 나비목 : 네발나비과), 팔공산밑들이메뚜기 (Anapodisma beybienkoi Rentz et Miller, 1971, 메뚜기목 : 메뚜기과)와 기후변화생물지표종 먹그림나비 (Dichorragia nesimachus Boisduval, 1836, 나비목 : 네발나비과), 암끝검은표범나비 (Argyreus hyperbius Linnaeus, 1763 나비목 : 네발나비과), 제주등줄박 각시 (Marumba spectabilis Okamoto, 1924, 나비목 : 박각시과)가 확인되었다. Shannon’s diversity 지수(H)와 Simpon’s diversity 지수(D′)를 이용하여 각 지역별 다양성을 산출하였는데 안면도 휴양림 (H=4.486, D′=0.9804)이 가장 높게 나타났으며, 대소산 (H=4.175, D′=0.9622)이 다음으로 높은 다양성을 보였다. 주성분분석(Principal Component Analysis) 결과 월별 종 구성의 차이는 크게 나타나지 않았지만, 지역별 구성에서 백화산, 안면도 휴양림, 대소산에서 다른 군집과 매우 상이한 종 구성을 보였다.