Physiological disorders such as symptoms in leaf colored with brown spots are so many occurred in ginseng garden cultivated with paddy soil. This study was carried out to inhibit the symptoms of brown-colored leaf in 3-year-old ginseng by fertilizing calcium hydroxide [Ca(OH)2] of 100 ~ 400 ㎏ per 10a on paddy soil before transplant of seedling. Soil pH was rapidly increased, while Fe was decreased in soil by the increase of application level of calcium hydroxide. Soil pH was increased from 4.53 to 6.18 when calcium hydroxide was fertilized at level of 100kg per 10a. The content of Fe in ginseng leaf was decreased more than the control by fertilizing calcium hydroxide in soil. Ratio of brown-colored leaf and plant height and leaf area were decreased by the increase of calcium hydroxide. Ratio of survived root and yield of root showed the peak at the application level of 100 ㎏ per 10a, and both of them were gradually decreased by the increase of calcium hydroxide. The decrease of missing plant rate above the application level of 200 ㎏ per 10a had a negative effect on the decrease of yield of root.

This study was carried out to evaluate the preventive effect of three forms of Korean ginseng roots (fresh, white and red) against bisphenol A (BPA) toxicity in mouse male germ cells (GC-2spd, TM3, TM4). ROS (reactive oxygen species) generation were measured by DCF-DA (2’,7’-dichlorohydrofluorescein diacetate) assay. Also, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was performed to quantify the mRNA expression levels of apoptosis- related genes, Bax (pro-apoptotic gene) and Bcl2 (anti-apoptotic gene). ROS generation was increased by 50 μM BPA, but definitely decreased by treatment with Korean ginseng extracts (fresh, white and red) in mouse male germ cells. In especial, Korean fresh ginseng extract reduced significantly ROS production to normal control. In addition, Korean fresh and white ginseng extracts suppressed the apoptosis of mouse male germ cells by fine-tuning mRNA levels of apoptotic genes changed by BPA. In general, Korean fresh ginseng extract was more effective than white ginseng extract for reducing BPAinduced oxidative stress and apoptosis in mouse male germ cells. Therefore, Korean fresh and white ginseng may help to alleviate biphenol A toxicity in mouse male germ cells.

Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and 100 μg/ml of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was 50 μg/ml of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.

An LED plant factory farm is an alternative way to grow crops regardless of weather, season, and blight in such times of climate change. In recent years, it is a currently active and vibrant research field. The industry, which ranges from leaf vegetables to high value products, is expanding. This study was conducted to test tthe response of LED (Lightemitting diode) irradiation on the growth characteristics and ginsenoside levels indoors, in order to find out suitable light conditions. Ginseng seedling was transplanted from a styrofoam pot (L×W×D:495×315×215 mm, inside diameter) into a closed plant production system in four blue LED (BL) and red LED (RL) different ratios of 1:1, 1;2, 1:3, 1:4 in a temperature range of 20~25℃, relative humidity of between 55 and 65%, and a 12-hour photoperiod. The LED irradiation shows the highest levels were found at 1:1 of BL and RL ratio at 61.21 μmol s-lm-2, 1:2 ratio 68.55 μmol s-lm-2, 1:3 ratio 63.85 μmol s-lm-2 and 1:4 ratio 62.41 μmol s-lm-2 from highest to lowest respectively. After analyzing from shoot and root 2 yers old ginseng plant which were cultivated under 1:3 irradiation of BL and RL ratio, it generally showed a positive effect under a 1:3 ratio of BL and RL.

Shading and soil environment are the main factors of growth and yield in ginseng (Panax ginseng C. A.Meyer). Ginseng yield is directly related to survival rate because of increased missing plant for their growing period. Underfield conditions, diseases and pests significantly affect plant survival rate. We evaluated the seedling establishment, growthand ginsenoside of the ginseng plants, under controlled management conditions in a plastic greenhouse, when their treatedwith different types of organic matter. Ginseng seeds were sown at a rate of three seeds per hole, and the seeding space mea-sured 10㎝×15㎝. Compared to the control, treatment of cattle manure vermicompost (CMV) was shown to increaseseedling establishment and decrease ginsenoside content. Root weights of plants treated with CMV were higher than those ofplants treated with other types of organic matter. In addition, seedling establishment of 2-year-old ginseng plants wasdecreased when it was compared to that of 1-year-old ginseng plants. Our results indicated that organic matter type and ratewere associated with seedling establishment, growth characteristic and ginsenoside content in greenhouse of ginseng direct-sowing culture.

고품질 우량인삼 생산을 위한 해가림자재별 인삼의 생육 상황과 해가림 아래의 미기상 변화를 조사하였고, 각각의 해가림자재에서 생산된 수삼의 유효성분들을 조사 분석한 결과, 6~8월까지의 해가림자재별로 온도는 차광지>차광판> 차광지 순이었고, 투광량도 차광지가 8월에 381.7 μmol/s/m2 로 인삼포내 온도가 가장 높은 원인이었다. 2~3년 인삼의 지상부 생육은 차광망>차광판>차광지 순이었으며, 4년근은 차광판=차광지>차광망 순으로 좋았다. 4년근 인삼의 진세 노사이드 총함량은 차광판>차광지>차광망 순으로 많았으 며, 수삼의 색도는 차광망>차광판>차광지 순으로 높았다

인삼 개갑종자의 초저온보존 효과 및 최적조건을 구명하기 위하여 건조 조건과 수분함량 및 액체질소 보존 전 동결처리와 보존 후 해동처리에 따른 초저온보존 효과를 조사하였다. 건조처리는 25℃ air flow 챔버(RH 10～12%)와 15℃ 건조실(RH 22～25%)을 이용하여 실시하였고 보존 전 동결은 –24℃에서 30일간 처리하였으며 보존 후 해동은 4℃에서 24시간, 40℃에서 2분 처리구를 각각 비교하였다. 그 결과 15℃에서 종자수분 8-12%로 건조된 종자가 보존 전 처리 없이 액체질소에 보존된 후 40℃에서 해동되었을 때 발아율이 가장 높은 것으로 나타났다. 그러나 종자수분이 2.2% 이하일 때 건조가 지속되면 발아율은 감소하는 것으로 나타났으며, 4% 이하에 이르면 보존 전후 처리효과는 나타나지 않는 것으로 조사되었다. 반면 25℃에서 건조된 종자는 상대적으로 높은 발아율을 유지하였다. 건조 및 초저온보존에 의한 인삼 개갑 종자의 유근 및 자엽 생장율을 조사한 결과, GA배지 치상 10일 후 무처리한 대조구에서 첫 유근이 출현하였으며 3주 후에는 자엽발달이 시작되었다. 30일째에는 자엽생장율이 유근발달과 함께 유의하게 증가하는 것으로 나타났다. 치상 30일까지 초저온보존에 의한 생장율은 같은 양상을 보이면서 대조구보다 현저히 낮게 나타났으나 40일째에는 양상은 같았으나 회복율이 대조구보다 증가세를 보였다. 또한 보존 전 동결처리 없었던 종자는 동결처리한 종자보다 모든 처리구에서 높은 유근 및 자엽 생장율을 보였다.

본 연구는 인삼점무늬병과 탄저병에 대한 친환경자재로 만들어진 친환경 살균제의 억제효과를 알아보기 위해서 수행을 했다. 인삼 점무늬병과 인삼 탄저병들은 인삼뿌리가 비대하기 시작하기 전에 낙엽을 시켜서 수확량을 크게 감소하는 주요한 병들이다. 천연칼슘, 녹차추출물, 홍삼박 술등은 A. panax에 의해서 유발된 인삼 점무늬병에 대해서 상당한 억제효과를 보였고 게 껍질 발효액과 익모초자연즙액은 C. gloeosporioides에 의해서 유발된 인삼탄저병에 대해서 큰 억제효과를 나타내었다. 이들 친환경 살균제는 치료효능보다 대체적으로 예방효능이 더 우수 하였다. 그러므로 이들 친환경 자재들은 인삼 유기재배포장에서 사용할 수 없는 화학농약을 대체하는 살균제로써 이용할 수 있고 그들의 억제효과를 극대화하기 위해 발병 전에 처리를 해야 한다.

Lime-bordeaux mixture (LBM) has been used instead of pesticides in ginseng field and orchard since the 1960's in Korea. In this experiment, LBM was made with different concentrations and sprayed in the field of ginseng for eco-friendly cultivation. Growth characteristics and disease such as alternaria blight, anthracnose, and gray mold were investigated in 4-5 year old ginseng after spraying LBM. LBM caused a little damage on leaf when it was sprayed at the time of leafing stage, late April and early May. Root weights of five-year-old ginseng were 43.1~51.5 g and 41.2~46.6 g in the plot of mid-April and mid-May treatments, respectively. These growth levels were further reduced as compared with that of the chemicals treatment plot. The rate of diseases in the plot of 6-6 and 8-8 ratio were 0.0~4.8% and 0.0~4.4%, respectively, which was similar with that in the plot of chemical control for alternaria blight and anthracnose. However, LBM had little effect on controling gray mold. It showed lower control effect in the plot of 4-4 ratio than that of chemical control. This result will be expected to be a useful guide that can be used in the field to the farmers of the ginseng.

This study was performed to investigate the effects of sowing density and number of seeds sown on the emergence rate and growth characters of Panax ginseng C. A. Meyer under direct sowing cultivation in a blue plastic greenhouse. Ginseng seedlings, derived from seeds sown directly at different densities (90, 108, 135, and 162 seeds per 162m2), were cultivated in sandy loam soil within a blue plastic greenhouse. In contrast to the emergence rate, which decreased with an increase of sowing density, number of survival plant showed an increasing trend. Interestingly, the emergence and number of survival plant were significantly enhanced when 2 or 3 seeds were sown per hole compared with when one seed was sown per hole. Growth of the aerial parts of ginseng were not markedly influenced by sowing density or the number of seeds sown. However, chlorophyll content (SPAD values) increased with an increase in sowing density. Root parameters, such as root length, diameter, and weight, and the number of lateral roots decreased with an increase in sowing density, but were not noticeably influenced by the number of seeds sown. Total saponin content was the highest in the treatment plot containing 135 seeds. Similarly, the content of each ginsenoside was also tended to be higher in this treatment than in other treatment plots. On the basis of the results obtained in this study, it was possible to determine the optimal sowing density and seed number for the direct sowing cultivation of ginseng in blue plastic greenhouse.

Anti-inflammatory activity of the extracts of ginseng berry (GBE) was investigated through the evaluation of its inhibitory effect on the production of inflammatory meditator, nitric oxide(NO), tumor necrocis factor-alpha (TNF-α), interleukin-6 (IL-6) in LPS-induced RAW264.7 macrophage cells. GBE was fractionated using n-hexane, chloroform, ethylacetate, buthanol and H2O, sequentially. RAW264.7 cells were induced 100ng/mℓ of lipopolysaccharide (LPS) and treated with 0, 1.6, 8, 40 and 200μg/mℓ of GBE fractions. LPS-induced NO production on all of GBE fractions was inhibited with increasing added concentration of GBE fractions. Chloroform fraction of GBE was the most effective in inhibiting LPS-induced TNF-α production. Hexane, chloroform and H2O fractions of GBE exhibit strong inhibition LPS-induced IL-6 production. Especially, H2O fractions of GBE was the most effective in inhibiting LPD-induced IL-6 production without significant cytotoxicity in RAW264.7 cells, and reduced the activation of mitogen-activated protein kinases (MAPK) and IkB phosphorylation. These results indicate that H2O fractions of GBE exhibits strong anti-inflammatory effects by inhibition of NF-kB by inhibition of p-38 on MAPK and IkB phosphorylation.

This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from a large number of samples.

This study was performed to identify optimal harvesting time of ginseng seeds and to examine the effect of GA3 treatment for improvement of seed stratification rate. Ginseng seeds harvested from Land race, Chunpoong and Yunpoong cultivar in July 20 were tested for stratification rate. It was shown that stratification rates of land race, Yunpoong and Chunpoong cultivar were 94.1%, 93.1%, and 82.6%, respectively. Seeds of Chunpoong cultivar harvested 10-15 days later showed a comparable stratification rate to that of Land race, indicating that late harvest of Chunpoong seeds is beneficial for the increase of stratification rate. The higher stratification rate was found in mature seeds (92.3%) than immature seeds (37.8%), both of which were harvested in July 20. Stratification rate of mature seeds harvested in July 15 was 87.5%, demonstrating optimal harvesting time of ginseng seeds with higher stratification rate is after mid-July. An exponential growth of endosperms of ginseng seeds was observed from early June to mid-June and then slow growth was observed. There was no obvious growth of embryos from fertilization to mid-August. After the this time, embryos quickly grew until late October. Thus, appropriate stratification control is essential during the period (from early September to late October) in order to optimize embryo growth and development. While no increase of stratification rate was observed in seeds treated with 50 ppm of GA3, significant increases were observed in seeds treated with 100 ppm of GA3. At this concentration of GA3, the stratification rate of Land race, Chunpoong and Yunpoong cultivar was 95.0%, 95.3%, and 96.5%, respectively.