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        검색결과 102,933

        541.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study examines public perceptions and language ideologies regarding English use in Korea‘s linguistic landscape by analyzing 2,191 online news comments concerning the English-only menu controversy in 2023. The analysis reveals that negative comments (89%) significantly outnumbered accepting ones (11%). Critical responses view English use as displaying pretentiousness and cultural subservience while posing a threat to Korean linguistic and cultural identity. Critics raise concerns regarding language use, such as incorrect English usage, inconsistent bilingual practices, and discrepancies between displayed and actual proficiency. They also point to issues of information accessibility and the broader trend of excessive English use in Korean society. By contrast, accepting perspectives justify English menu use based on business owners’ autonomy, strategic marketing, the basic comprehensibility of the English used, and the naturalness of English use in the global era. The findings highlight that monolingualism prevails, with English perceived as distinctly “foreign,” while also revealing contradictions in public attitudes, including overreliance on foreign validation of Korean, conflicting views on linguistic hierarchy, and ambivalent attitudes toward English proficiency. This study contributes to a better understanding of public perceptions of English in Korea‘s linguistic landscape and the ideological dynamics underlying language choice in public spaces.
        7,000원
        542.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The 2020 revision of Japan's Wholesale Market Act aimed to improve the financial performance of wholesale companies. Before the revision, the wholesale market saw a continuous decline in transaction volume and value, and the operating profit margin of wholesale companies was 0.18% in 2018, with 38.2% of wholesale companies experiencing operating losses. After the revision of the Wholesale Market Act, the operating profit margin of wholesale companies significantly improved to 0.41% by 2022. The reasons for this improvement are as follows: The transaction amount and the rate of change between 2022 and 2018 showed little difference. The number of markets increased by one central wholesale market, and while the number of buying and selling participants decreased, individual purchase volumes increased. The proportion of auctions further declined, and the number of direct transactions rose. As consignment collection decreased, purchased collection increased, leading to an improvement in financial performance. Ultimately, despite the short time frame, the revision of Japan's Wholesale Market Act led to positive changes that improved the financial performance of wholesale companies.
        4,500원
        543.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study conducted to investigate potential differences in the activity of genes involved in volatile fatty acid (VFA) absorption, pH regulation, and energy metabolism in epithelial cells of forestomach administered either endophyte-infected (E+; 4.45 mg ergovaline/kg) or endophyte-free (E−) tall fescue seed. Twelve steers [body weight (BW) = 547 ± 9 kg] were fed alfalfa cubes at 1.5 × NEm and dosed 1 kg of ground tall fescue seed daily via rumen cannula for 21 days. On day 22, steers were slaughtered, and tissue samples were collected from the rumen, reticulum, omasum, and abomasum. Gene expression analysis revealed that monocarboxylate transporters (MCT), isoform 1 and MCT4 expression levels were significantly lower (p<0.05) in the rumen epithelium of steers dosed with E+ seed, while MCT2 expression remained unchanged. Similarly, sodium hydrogen exchanger (NHE), isoform 2 expression was significantly reduced (p<0.05) in the E+ seed, whereas NHE1 and NHE3 were unaffected by the seed treatment. Additionally, expression levels of down regulated in adenoma (DRA) and anion exchanger (AE), isoform 2 were lower (p<0.05) in the rumen epithelium of E+ steers, while putative anion transporter 1, sodium bicarbonate cotransporter, isoform 1, 3-hydroxy 3-methylglutaryl coenzyme A synthase, isoform 2, and sodium potassium ATPase pump, isoform 1 expression levels were not influenced by the seed treatment. Notably, gene expression in the reticulum, omasum, and abomasum epithelia was unaffected (p>0.05) by seed exposure. These findings suggest that endophyte-infected tall fescue seed may impair ruminal VFA absorption in its dissociated state (pH > 5.8) by downregulating MCT1 and MCT4, along with suppressing NHE2, DRA, and AE2. Therefore, this mechanism may partially explain the reduced weight gain associated with fescue toxicosis in cattle.
        4,000원
        544.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study developed an integrated performance evaluation framework for rural development Official Development Assistance (ODA) projects in Korea and validated its effectiveness through practical application. Based on the FAO’s SAFA (Sustainability Assessment of Food and Agriculture Systems) Tool, the framework enables a balanced assessment across economic, social, environmental, and governance dimensions. The methodology incorporates the Analytic Hierarchy Process (AHP) and Importance-Performance Analysis (IPA) to measure the importance and performance of objectives and indicators. The developed framework serves as a tool for Project Design Matrix (PDM) development, monitoring, and evaluation throughout the project’s planning, implementation, and completion phases. During planning, it systematically incorporates stakeholder input in setting objectives and indicators. During implementation, it facilitates real-time monitoring for immediate decision-making and resource reallocation. At completion, it supports comprehensive performance evaluation. Application of the framework to the “Rural Development Programme in Tuyen Quang Province, Vietnam” demonstrated its effectiveness in systematizing excessive indicators and clarifying the hierarchical and logical connections between objectives. This performance evaluation framework can enhance project transparency and accountability by overcoming the limitations of current PDM approaches and providing systematic methods for incorporating stakeholder feedback. It is particularly applicable to multi-sectoral rural development programs and is expected to contribute to integrated development in target areas. However, validation through a single case study presents limitations, necessitating future application across diverse regions and project types to verify generalizability.
        4,800원
        545.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        A 13-year-old female lion underwent a simple mastectomy for a rapidly growing abdominal mass, confirmed as mammary adenocarcinoma after histopathological examination. Mammary tumors are common in domestic cats but less frequently reported in African lions. This report presents the first documented case of mammary adenocarcinoma in an African lion in South Korea. The mass was successfully excised through stable anesthesia and surgery. This report adds to the limited literature on mammary tumors in large felids and discusses the need for tailored management strategies.
        3,000원
        546.
        2025.03 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        This research, grounded in the extended technology acceptance model, aimed to explore the relationships among factors influencing Korean EFL learners’ acceptance of ChatGPT for English learning in a voluntary usage context. To this end, a questionnaire was distributed to college students who had used ChatGPT for language learning, utilizing a convenience sampling method. A total of 400 responses were analyzed to test hypotheses using structural equation modeling (SEM). Findings revealed that learners’ perceived usefulness significantly predicted their intention to continue using ChatGPT, while perceived ease of use did not. Moreover, learners’ result demonstrability was found to be a predictor of perceived usefulness, whereas subjective norm was not. Both playfulness and output quality significantly influenced learners’ perceived ease of use. This study identified key factors that could enhance EFL learners’ acceptance of ChatGPT by improving perceptions of usefulness and ease of use, offering valuable insights for integrating ChatGPT into English education.
        6,300원
        547.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        목적 : 본 연구는 세륨(IV)-지르코늄(IV) 산화물 나노입자를 사용하여 콘택트렌즈를 제조한 후, 안의료용 기능성 렌즈로의 사용 가능을 확인 위해 제조된 렌즈의 물성을 비교 분석하였다. 방법 : 2-Hydroxyethyl methacrylate에 나노 세륨-지르코늄 산화물(cerium(IV)-zirconium(IV) oxide)을 첨가하여 공중합 한 후 물성을 측정하고, 친수성 단량체인 methacrylic acid(MA)를 추가로 첨가하여 물성을 측정, 비교하였다. 결과 : 다양한 비율의 세륨(IV)-지르코늄(IV) 산화물 나노입자와 MA를 첨가한 렌즈의 물성을 평가한 결과, UV-B 투과율은 40.95~66.26%, 굴절률 1.4163~1.4357, 함수율 37.44~47.18%, 접촉각 36.87~56.36°, 인장 강도 0.0612~0.561 kgf/mm², 표면거칠기 7.70~8.72 nm로 각각 측정되었다. 나노입자 및 MA 첨가는 습윤성, 인장강도 및 중합안정성을 향상시키고, UV-B 투과율과 표면거칠기를 감소시켰으며, 황색포도상구균에 대한 항균 성이 확인되었다. 결론 : 세륨(IV)-지르코늄(IV) 산화물 나노입자에 MA를 첨가하여 제조한 렌즈가 중합 안정성, 내구성, 습윤성 을 향상시키는 것을 확인하였으며, 따라서 안의료용 기능성 콘택트렌즈 소재로 활용할 수 있을 것으로 판단된다.
        4,200원
        548.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aimed to evaluate the effects of different feeding levels of domesticated barnyard millet and imported Bermuda hay on the growth performance and structural development of female and male growing goats. A 4×4 Latin square design was used, involving 8 goats with an average age of 3 months: 4 females and males with an initial body weight (BW) of 10.6 kg and 16.0 kg, respectively. Goats were randomly assigned to 4 dietary treatments: T1 (1.5% BW barnyard millet), T2 (1.5% BW Bermuda hay), T3 (2.0% BW barnyard millet), and T4 (2.0% BW Bermuda hay) over a 22-week period. Results indicated that the highest final body weight (FBW) was significantly observed at the highest feeding level (T4), with females reaching 14.9 kg and males 20.9 kg, while the highest average daily gain (ADG) values were recorded for females in T3 at 75.7 g/d and males in T4 at 81.0 g/d (p<0.05). Dry matter intake (DMI) was highest in the T4 group for both females (437.4 g/d) and males (635.9 g/d), with significant differences observed across treatments (p<0.05), whereas the feed conversion ratio (FCR) showed an improving trend, particularly with a value of 6.0 for females in T4. For structural development, the highest feeding levels led to significant increases in body length, body depth, chest width, and chest girth of both sex. Female and male in T4 achieved body lengths of 53.5 cm and 61.8 cm, and body depths of 45.2 cm and 54.8 cm, respectively. Chest width and girth reached 15.9 cm and 66.5 cm in males, and 13.5 cm and 56.5 cm in females. In conclusion, higher feeding levels, especially with Bermuda hay, may positively influence the growth performance and structural development of goats.
        4,200원
        549.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study examines the price elasticity of demand for mackerel in the Busan Cooperative Fish Market, the largest wholesale fish market in South Korea. Using a two-stage least squares (2SLS) approach, the analysis addresses endogeneity in pricing by incorporating exogenous environmental variables, such as sea surface temperature (SST) and wind speed. The study estimates demand elasticity for three size categories of mackerel-large, medium, and small-and reveals significant differences across these categories. Large-size mackerel exhibits inelastic demand (-0.875), reflecting its status as a staple product with relatively stable consumer demand. Medium-size mackerel shows highly elastic demand (-2.450), likely due to its role as a substitute for both large and small mackerel. Small-size mackerel also demonstrates high elasticity (-3.444), attributed to its primary use in feed and processing, where demand is particularly sensitive to price changes. Diagnostic tests confirm the validity and relevance of the instrumental variables, with SST and wind speed strongly correlated with price but uncorrelated with consumer demand. These findings highlight the critical role of size-specific market characteristics in shaping price elasticity and provide valuable insights for policymakers and industry stakeholders to better manage mackerel supply and ensure pricing stability.
        4,600원
        550.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, the safety and efficacy of an spot on formulation composed of Fluralaner (40mg/kg)and Moxidectin (2mg/kg) against internal and external parasites was evaluated in Korean cats. The product was developed as a topical use in cats to prevent or control internal and external parasites including ticks, fleas, gastrointestinal parasites, ear mite and heartworm. As a result of the study, targeted for total 30 cats from 8 Korean animal hospitals, this combination of Fluralaner and Moxidectin was found to be very safe in cats, and its effectiveness against external parasites (ticks, fleas, and ear mites) was fully verified. In addition, As the animals exposed in the infective circumstances of heartworm and gastrointestinal parasites were remained negative during the study period of 12 weeks after treatment of test drug, it was determined that this product had a potential preventive effect against the heartworms and gastrointestinal parasites.
        4,000원
        551.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        이 논문은 대종교(大倧敎)의 􋺷단군교오대종지포명서􋺸에 수록된 창세신화를 중심으로, 여기에 드러난 하늘과 인간에 대한 인식을 천인 관계의 전통 속에서 파악한 것이다. 􋺷단군교오대종지포명서􋺸의 창세신화에서 우주의 창조는 정(精), 기(氣),신(神)을 바탕으로 한 도교의 우주론과 유사하다. 또한 하늘과 신의 세 차례에 걸친 인류 창조와 신의 문화 창조는 기존의 천인 관계 사이에 신을 배치 하여, 신과 인간의 직접적인 연결을 강조하였다. 창세신화에서 하늘과 신의 인 류 창조는 불완전하며, 이 불완전한 창조를 대황조성신이 완성한다. 이는 대황 조성신의 위상을 명확하게 하고, 대황조성신의 직계 자손인 대종교도들이 ‘오대 종지’를 지켜야 할 근거가 된다. 나아가 대종교 교리의 전제가 된다. 이렇듯 기 존에 전승되던 단군 신화에 더해 인류의 기원과 우주의 기원을 구체화한 􋺷단군 교오대종지포명서􋺸에 수록된 창세신화는 나라를 잃어버린 대종교도들에게 모 순된 현실 아래에서 하늘과 신, 단군으로부터 자신에게까지 이어지는 우주의 통 합을 이루도록 만들어준다. 따라서 대종교도들이 이 창세신화를 기억하고, 이를 기반으로 의례를 수행하는 것은 20세기 대종교도들에게 무너져버린 현실을 대 황조성신의 도로 새롭게 갱신하는 순간이 된다.
        6,000원
        552.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study evaluates the determinants of Cambodia’s agricultural product exports from 2004 to 2023 across 35 major export destinations using the gravity model approach. During this period, China accounted for the largest share of export value, followed by Vietnam, France, Malaysia, and Thailand. Cambodia’s GDP and the GDP of its trading partners positively influenced the country’s agricultural exports. Moreover, the historical status of export destinations as former French colonies facilitated Cambodia’s export flows. In contrast, exchange rates and geographical distance negatively affected Cambodia’s agricultural trade. This study confirms the existence of significant untapped export potential between Cambodia and its trading partners. To optimize its agricultural exports, Cambodia can benefit from processing agricultural products before export. Therefore, the Royal Government of Cambodia (RGC) should actively support and promote investment in the agricultural processing industry to enhance the country’s international competitiveness. Additionally, the RGC and policymakers should strategically select export destinations through favorable trade negotiations and focus on the effective implementation of existing export policies.
        4,900원
        553.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Cool-season grasses are currently widely used in South Korea, but their productivity and quality may decline due to global warming associated with climate change. Warm-season grasses have shown greater adaptability to heat stress and drought conditions, making them potential alternatives for summer pasture establishment in central and southern Korea. This study aimed to compare the growth characteristics, productivity, and forage value of warm-season grasses bermudagrass and crabgrass for pasture establishment in the central region of South Korea. The experiment was conducted from May 2023 to October 2024 at the National Institute of Animal Science, Cheonan, South Korea. Crabgrass exhibited superior initial establishment and faster growth rates, whereas bermudagrass had poor initial growth, placing it at a competitive disadvantage against weeds. However, after the first cutting, bermudagrass successfully established itself and maintained a stable botanical composition. The annual dry matter productivity of crabgrass (8,586 kg/ha in 2023 and 12,013 kg/ha in 2024) was 32.62% and 40.18% higher, respectively, than that of bermudagrass (5,785 kg/ha in 2023 and 8,570 kg/ha in 2024). In forage value, crabgrass tended to show higher feed value at the initial harvest, while bermudagrass showed higher quality at the later harvests. In conclusion, these results suggest that both crabgrass and bermudagrass have potential for pasture establishment, with carbgrass being more suitable for short-term due to its rapid growth and high productivity, and bermudagrass being more appropriate for long-term due to its stable productivity in regions where overwintering in possible.
        4,000원
        554.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        국내 마약사범은 2022년 18,395명, 2023년 27,611명 등 폭발적으로 증가하고 있고, 정부는 권역별 마약범죄 특별수사본부 설치, 공항 마약 검색 강화 등 단 속과 수사를 강조하고 있다. 현재 해상에서 발생하는 마약 사건 비율이 높지는 않으나, 대한민국은 삼면이 바다로 둘러싸여 있는 점, 해양이 대체 마약 운송 루트로 활용될 가능성 등을 고려할 때 리딩 판례를 토대로 향후 수사 과정에서 유의할 점을 점검하고 제도 개선 방향을 검토할 필요성이 있다. “2019년 태안 해상에 정박해 있는 외국 국적 선박에서 코카인 약 100kg을 압수하고 일등항해사를 기소한 사례”에서 법원은 1) 통역 내용을 신뢰하기 어 려운 점, 2) 소변, 모발, 압수한 휴대폰 등에서 마약 관련 증거가 발견되지 않는 점 등을 이유로 무죄를 선고하였다. 해상 마약 사건에서 유사한 문제가 반복되 는 것을 방지하기 위하여 수사기관과 법원에 산재되어 있는 통역인의 통합관 리, 통역 인증제 확대 등을 통해 통역 자원의 양과 질을 늘리고, 이온스캐너 등 현장에서 신속히 마약을 검출할 수 있는 도구 보급에 힘써야 한다. 또한, 선주 에 마약 반입 예방을 위한 보안 의무 부과, 선박 압수 규정 신설, 국제공조 활 성화 등 전방위적인 노력이 수반되어야 한다.
        8,100원
        555.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The role of the gut microbiota in colorectal cancer (CRC) development has garnered attention, highlighting probiotics as potential adjuncts in CRC prevention and treatment. In recent years, probiotics and their derivatives have demonstrated mechanisms that may contribute to anticancer properties. This study investigates the cytotoxic effects of Bifidobacterium bifidum KCTC 3357, Lacticaseibacillus rhamnosus KCTC 5033, Limosilactobacillus reuteri VA 103, Bacillus galactosidilyticus VA 107, and Lactococcus taiwanensis VE101 on CT-26 mouse colon carcinoma cells using live cells, heat-killed cells (paraprobiotics), and cell-free supernatants (CFS, postbiotics) through an MTT assay. The results indicate that live bacterial strains, such as KCTC 3357, VA 103, and VA 107, promoted CT-26 cell viability, while heat-killed cells and CFS exhibited dose-dependent cytotoxicity. Inactivated forms of KCTC 3357 and VE 101, as well as CFS at 10 mg/mL concentration of KCTC 5033, VA 103, and VE 101, showed the strongest antiproliferative effects. These findings suggest that non-viable probiotic derivatives, such as paraprobiotics and postbiotics, offer promising therapeutic potential for CRC, providing a safer and more stable alternative to live probiotics. However, further research is required to explore their mechanisms of action, in vivo efficacy, and potential clinical applications.
        4,000원
        556.
        2025.03 KCI 등재후보 구독 인증기관 무료, 개인회원 유료
        Sympathetic innervation stimulates β-adrenergic receptors, triggering cyclic adenosine monophosphate (cAMP) production and enhancing protein secretion in salivary gland cells. While cAMP signaling, in conjunction with Ca2+ signaling, is essential for salivary gland function, the identified cAMP-producing G-protein-coupled receptors (GPCRs) remains limited. Here, we report the presence of cAMP-producing adenosine receptors in salivary gland cells. By reanalyzing publicly available single-cell transcriptome datasets of human and mouse submandibular glands, we identified mRNA expression of adenosine A1, A2A, A2B, and A3 receptors. Additionally, we confirmed that 5’-N-ethylcarboxamidoadenosine (NECA), an adenosine A2B receptor agonist, increases cAMP levels in human salivary gland cells, suggesting a physiological role for adenosine A2B receptors. Our findings enhance understanding of adenosine’s regulatory function in salivary glands and highlight new avenues for research on cAMPproducing adenosine receptors.
        4,000원
        557.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        목적 : 본 연구는 근시에서 굴스트란드 모형안에 편심 렌즈를 적용했을 때의 광학적 효과를 분석하고자 하였다. 방법 : 굴스트란드 모형안은 3D 시뮬레이션 프로그램을 사용하여 정밀하게 설계하였으며, 근축 근사 없이 정확 한 분석을 제공하는 광선 추적 기술을 적용하여 편심 렌즈로 인한 초점거리와 굴절력의 변화를 정밀하게 조사하 였다. 결과 : 렌즈 중심축의 편심이 초점거리, 굴절력, 광 경로 차이에 미치는 영향을 다양한 편심 조건에서 분석하였 으며, 특히 중심축으로부터 일정 높이에서 평행광선이 입사하는 경우를 집중적으로 조사하였다. 광선의 입사 높이 가 극도로 커짐에 따라 편심의 효과는 감소하고, 출사 광선의 초점은 서로 가까운 지점으로 위치하는 것이 확인되 었다. 렌즈와 눈의 굴절력과 편심에 따른 프리즘 굴절력 효과를 포함하는 전체 굴절력은 광선의 입사 높이가 감소 함에 따라 크게 변화하는 것으로 나타났다. 결론 : 본 연구 결과는 사시 및 사위와 같은 시각적 이상을 교정하기 위해서, 의도적으로 편심된 렌즈를 설계하 고 처방하는 데 유용한 정보를 제공할 수 있을 것으로 기대된다. 또한, 편심 렌즈 설계 시 발생할 수 있는 비선형적 광학 효과를 최소화하여 사용자에게 최적의 시각적 성능 제공을 보장할 수 있을 것으로 기대된다.
        4,300원
        558.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Maize (Zea mays. L) is one of the major sources of green fodder for livestock in Pakistan. Crop management plays a key role in obtaining high yields for green fodder. Fertilizer application, seed rate, and row spacing are critical components of crop management, which can significantly affect crop biomass. To determine the best production technology, a two-year (2021-2023) study was conducted at the research area of National Agricultural Research Center, Islamabad. Plant height, number of leaves, leaf area, green fodder yield per acre, and green fodder yield per hectare were recorded. Various row spacing (15 cm, 30 cm, 45 cm, and 60 cm), fertilizer ratio (N: P = 55:30, 65:40, 75:50, and 85:60), and seed rates (30 kg/ac, 35 kg/ac, 40 kg/ac, and 45 kg/ac) were applied. Results obtained experiments revealed that in both growing seasons, the maximum green fodder yield was obtained when fertilizer N: P ratio was 75:50 (green fodder biomass: 74.61 t/ha and 72.56 t/ha). Similarly, the optimal seed rate was found to be 40 kg/ac, which resulted in the highest green fodder yield (73.41 t/ha and 72.88 t/ha in two seasons). Furthermore, the plant of maize at row spacing of 30 cm was found to generate the maximum green fodder yield (72.39 t/ha and 72.40 t/ha, respectively). Green fodder yield per hectare was found to be positively correlated with plant height, number of leaves, and leaf area. These findings underscore the significance of applying a fertilizer ratio of N: P = 75:50, a seed rate 40 kg/ac, and a row spacing of 45 cm for higher yields of green fodder in maize crop.
        4,000원
        559.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Gentamicin is an aminoglycoside antibiotic effective against aerobic gram-negative bacteria and is also used in veterinary medicine, particularly in the swine and bovine industries. However, no gentamicin product is currently approved for treating equine diseases in Korea. The present study aims to examine the time-dependent residue of gentamicin in horses after intravenous injection (IV) via jugular vein. The test product was injected at 6.6 mg/kg BW via jugular vein in nine horses. Blood was collected from the horse's jugular vein at 15 minutes, 30 minutes, 1, 4, 8, 12, 24 and 48 hours after injection. To purify the gentamicin in serum, 100μL of 20 mM HFBA in DW, 100 μL of 30% trichloroacetic acid and 300 μL of 20 mM heptafluorobutyric acid (HFBA) in acetonitrile (ACN) were added to 500 μL of serum and supernatant was applied to LC-MS/MS after centrifugation. LC-MS/MS-8050 analyzed the level of gentamicin in serum with Electrospray ionization (ESI) and multiple reaction monitoring (MRM) positive mode. Gentamicin C1 was 478 m/z and product ions were 322, 157 m/z. Precursor ion of Gentamicin C1a was 450 m/z and product ions were 322, 160 m/z. Precursor ion of Gentamicin C2 and C2a was 464 m/z and product ions were 322, 160 m/z. The LC column was a C18 and mobile phase composed of 20 mM HFBA in 5% ACN and 20 mM HFBA in 50% ACN. The amount of gentamicin was calculated by adding four components of gentamicin (C1, C1a, C2 and C2a). The pharmacokinetic parameters of gentamicin were calculated by the WinNonlin program. The Cmax of gentamicin in horse serum was 93 ± 17 μg/kg and the Tmax was 0.25 ± 0 hours. The T1/2 was 6.41 ± 2.32 hours and the CLt was 0.05 ± 0.01L/hr/kg. The Vd was shown as 0.44 ± 0.13 L/kg and the MRT was 1.98 ± 0.55 hours. In conclusion, our data provides useful pharmacokinetic parameters for gentamicin in horses following IV injection.
        4,000원
        560.
        2025.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This experiment was carried out to study the effect of elapsed time after air flow cutoff on the germination rate of Italian ryegrass seed with different moisture contents during natural drying on reclaimed land, Jangheung and Kimje of Korea from 2023 to 2024, respectively. Seeds with moisture contents of 15.3, 22.3 and 28.0% were placed in vinyl bag (30 × 40 cm) at storage thicknesses of 10, 15, and 20 cm, and air flow was cutoff for 48 h. Seed moisture content, seed temperature (℃) and germination rate were investigated at 12-h intervals. After 48 h of airflow cutoff during natural drying, seed moisture content did not significantly differ among storage thickness treatment (p>0.05). When Italian ryegrass seeds with moisture contents of 27~28% were stored under conditions with air flow cutoff at 15~20cm thickness for 48 h, the seed temperature reached up to 30℃ and the germination rate was excellent at around 70~80%.
        4,000원