Background : Miscanthus sinensis is a diploid hybrid and a temperate, perennial, cross-pollinating grass used as bioenergy plant, biomass production and high quality cellulose and ethanol production. This study was to carried out to investigate the expression of MsCOMT gene and the variation of lignocellulosic component and phenolic compounds contents in transgenic plants. Methods and Results : Multiple bands were detected from the homologous region of the COMT gene in PCR analysis. In order to obtain more detailed results, putative transgenic lines were estimated by RT-PCR analysis to confirm the expression of mRNA. Also, analysis of the lignin, cellulose, hemicellulose, and phenolic compound contents of transgenic Miscanthus plants were performed. Total lignin content of transgenic plants was lower than that of the control plant due to reduced caffeic acid O-methyltransferase (COMT) gene expression related to lignin production. Cellulose and hemicellulose contents in transgenic plants were not increased. Variation in cellulose and hemicellulose contents had no correlation with variation in lignin content of transgenic plants. Conclusion : In conclusion, transgenic M. sinensis was obtained with down-regulated COMT gene. Lignin synthesis was decreased what offers possibility of crop modification for facilitated biofuel production.

A total of 15 different corn hybrids, Kwangpyeongok, Gangdaok, Yanganok, Singwangok, Jangdaok, Cheonganok, Cheongdaok, Andaok, Dapyeongok, Pyeongkangok, Pyeonganok, Daanok, Sunwon 184, Gangilok, and P3394 was used to investigate the growth and yield depending on the sowing date. The sowing dates were April 5, June 25, and July 5 and each experiments was performed in triplicste. The growth of Gangdaok was the highest. However, although the growth of Kwangpyeongok, was lower thanthar of Gangdaok, its stem height to ear height ratio was lower than that of Gangdaok, thus , Kwangpyeongok may be more suitable for stable cultivation. Both growth and yield of Daanok were low, regardless of planting date, but yield and ear shape of Pyeongkangok and Dapyeongok were for fresh corn. Growth and yield of the 15 different corn hybrids varied depending on the planting date, However, the growth degree days (GDD) was the most important factor governing the maturity of corn. More than 1500°C of GDD was sufficient to harvest mature corn hybrids in the central region of Korea. Besides yield and growth, other characteristics, such as sweetness and taste of the hybrids, should be investigated further the selection of the best corn hybrid.

Nonlinear dynamic analysis of Non-seismic Designed subway station was verified with Channel Beam Damper system which was developed in former research. As a result of nonlinear dynamic analysis, column and beam are on LS(Life Safety) level by retrofitting with Channel Beam Damper system. The effect of Channel Beam Damper System was confirmed.

To investigate skin-whitening effect of Adenophora triphylla var. japonica sprout extract, antioxidant activity, inhibitory effect on tyrosinase and melanin synthesis in B16/F10 melanoma cell were examined. Total phenolic content (246.25 ㎎ GAE/g) and total flavonoid content (303.94 ㎎ RE/g) of ethyl acetate fraction from Adenophora triphylla sprout (EFAT) showed the highest contents than other fractions (n-hexane, chloroform and distilled water). Antioxidant activities of EFAT has been evaluated using ABTS, DPPH radical scavenging activities, FRAP and inhibitory effect of lipid peroxidation. EFAT showed excellent radical scavenging activity and inhibitory effect on MDA production. Inhibitory effect of tyrosinase as a major enzyme of melanin synthesis was also measured. In these results, EFAT showed higher inhibitory effect against L-DOPA (51.27%) than L-tyrosine. IC50 value on α-glucosidase was 41.93 ㎍/㎖. In B16/F10 melanoma cells, EFAT inhibited melanin synthesis at 200 ㎍/㎖ concentration (about 42% decrease). Finally, main physiological compounds of EFAT were identified as a rutin and a chlorogenic acid using high performance liquid chromatography.

Background : Sorghum is a major cereal food crop used in many parts of the world. It has been grown on a subsistence level by farmers, under various conditions of environmental stresses in the semi-arid tropics of Africa and Asia. This crop has received significant attention because of its ability to lower cholesterol in the blood, and its anti-dementia, antioxidant, and antimicrobial properties. It is possible to develop a functional and commercially viable sorghum variety by using superior cultivars of sorghum. The objective of this study was to construct a database of superior sorghum accession. Methods and Results : We used 250 sorghum accessions collected from different geographical bioregion in Korea. We determined various growth characters including germination rate and ear length of these accessions. To determine the antioxidant capacity, we measured the DPPH radical scavenging activity, ABTS scavenging activity, total phenolic contents, and total flavonoid contents. 189 accession showed higher germination (> 80%) than other accessions. Higher DPPH radical scavenging activity was observed in 11-SB-078 (RC50: 1.89 ± 2.88 ㎍/ ㎖), and higher ABTS scavenging activity was recorded in 11-SB-116 (RC50: 35.48 ± 2.42 ㎍/㎖) than other accessions. The ear length ranged from 15 ㎝ to 48 ㎝, the total phenolic contents ranged from 3 ㎎·GAE/g to 77 ㎎·GAE/g, and total flavonoid contents ranged from 0.09 ㎎·QE/g to 1.07 ㎎·QE/g. Conclusion : Among 250 sorghum accessions, we selected 10 with both superior agronomic characters and higher functional food quality.

Background: The light emitting plasma (LEP) has recently attracted attention as a novel artificial light source for plant growth and functional component enhancement. We investigated the effects of LEP on whitening and antioxidant activities of the plant parts of perilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and tyrosinase inhibition activity as indicators of biological activity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total flavonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power values of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of biological activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.

Blast resistance of 29 rice cultivars confirmed as a durable resistance in the evaluation of sequential planting from 2004 to 2006 was evaluated to nursery screening in 14 test sites during 11 years in Korea. The average disease severity (ADS) of 29 rice varieties against rice blast showed 3.5 degree; however, the difference of disease severity among the varieties was from 1.9 to 4.8. The 29 varieties were grouped into resistance less than 3.0 ADS degree including 12 varieties and moderate resistance over 3.1 ADS degree including 17 varieties. Among the 12 rice cultivars presented low ADS, 4 rice cultivars, Ungwang, Pungmi 1, Sinunbong 1, and Dasan 1 were constantly appeared high resistant reaction during 11 years in all test sites and the others were showed various diseases severity across the test years and the test sites. Twenty-one rice cultivars including Gopum were more variable among the test sites while the others were higher variable among the test years. These results indicated that durable resistance test against rice blast using sequential planting is a very efficient screening method to predict durability and nursery test for long periods and also useful method to predict indirectly durable resistance of rice cultivars.

浩亭 河崙은 고려 말기부터 조선 초기에 걸쳐 仕宦한 고위관료이다. 조선 개국, 이방원의 등극, 조선의 제도와 典章의 확립 등 중요한 국가 대사를 맡아 주도하였다. 그의 고위관료로서의 자취 때문에 문학적인 면모는 소홀히 여겨져 지금까지는 연구한 바가 없어 밝혀지지 않았지 만, 그는 실로 최고의 문학가였다. 그는 조선 초기 최고의 성리학자이고 문장가였다. 慶會樓의 記文을 지었고, 圃隱 鄭夢周, 惕若齋 金九容 등 당시 최상급 문인들의 시집 서문을 썼다. 그리고 고려 말 대문호로 그의 스승인 牧隱 李穡의 神道碑 銘을 지었다. 과거를 통해 출사한 文臣들이 직책에 맞는 능력을 갖추지 못 한 것을 보고, 重試를 실시하여 능력을 보강하려는 제도를 만들었다. 호정은 4번 明나라를 다녀옴으로써 그 당시 문화 교류에 있어 가장 큰 공헌을 했다. 鄭道傳이 경솔히 지은 表文이 明 太祖의 비위를 거슬렀 을 때, 직접 명 태조를 만나 상세히 분명하게 전후사정을 간곡하게 진술하여 문제를 잘 해결하고 왔다. 또 建文帝와 永樂帝의 즉위식에 참석하 였다. 돌아와서 지어 太宗에게 바친 ｢覲天庭｣, ｢受明命｣ 두 악장은 나중 에 명나라 영락제에게도 알려져 외교에 많은 도움을 주었다. 그는 많은 시를 지었으나 대부분 없어지고 문집에 실려 있는 시는 조선후기에 와서 수집한 시이다. 그 가운데 ｢漢江詩｣는 漢陽 천도를 송축하는 시인데, 그 속에 임금님을 諷諫하는 교훈이 들어 있다. ｢嶺南樓｣시는 절경 속의 嶺南樓가 名勝임을 부각시키고, 영남루에 대한 애정을 담았다. 곧 성리학자들의 天人合一 사상이 배어 있다. ｢慶會樓記｣는 경회루의 중복과정을 서술하고 중건과정에서 君民一 體가 되는 조선 초기 건전한 국가적 氣運을 느낄 수 있고, 또 경회루의 건물 구조 하나하나에 治道의 의미를 부여한 독특한 문장이다. ｢矗石樓記｣도 촉석루에 올라 풍경을 보면서 牧民官이 백성을 위해 해야 할 일을 연상하도록 하여 단순한 물리적 건물로서의 촉석루가 아니고, 목민관이 백성을 다스릴 방안을 창출해 내는 정신적 생산 공간 으로서 의미를 부여했다. 浩亭의 시나 문장은 단순한 문예적인 시문에 그친 것이 아니고, 經世 濟民의 의미를 담은 전통 유학자의 충실한 문학이다.

This study is aimed at identifying the distribution of vascular plants growing at Byeollipsan(Mt.), Bongchunsan (Mt.) and Gucksoosan(Mt.) in Ganghwa isand. Field surveys were conducted for each season from March 2015 to October 2015. The flora of study area were found to be of 455 taxa, 93 families, 280 genus, 392 species, 4 subspecies, 53 varieties and 6 forma. Rare plants were found to be of 3 taxa. Among them, rare plant species consisted of endangered species (EN degree):1 (Thalictrum simlex var. brevipes Hara), vulnerable species (VU degree): 2 (Wikstroemia trichotoma (Thunb.) Makino, Scorzonera albicaulis Bunge), respectively. In all the surveyed areas, a total of 6 taxa (Clematis brachyura Maxim. etc.) were found to be endemic to Korea. The floristic special plants found in the surveyed areas were 1 taxa of grade Ⅴ, 4 taxa of grade Ⅳ and 8 taxa of grade Ⅲ, respectively. The naturalized plants were identified as 29 taxa and included Fallopia dumetorum (L.) Holub, Ambrosia artemisiifolia L., Aster pilosus Willd. etc..

This study was conducted to investigate the antibacterial activities and sensory properties of a sauce containing tea extract and garlic vineger against raw fish. The sauce was evaluated antibacterial activity against Vibrio parahaemolyticus and Vibrio vulnificus which is considered as one of the major food borne pathogens. As a result, the sauce inhibited the growth of V. parahaemolyticus and V. vulnificus distinctly. In addition, the sauce with soy sauce and Kochujang showed the restricted growth of Vibrio parahaemolyticus and Vibrio vulnificus in sliced raw flatfish. Sensory evaluations were measured by 7-point hedonic scale using sliced raw flatfish with the sauce mixed with Kochujang and soy sauce. The score of texture in the sauce with Kochujang was significantly higher than that of regular Chokochujang (p<0.05). Moreover, total preference including appearance, texture, aroma and taste were significantly higher in the sauce with soy sauce than those of regular soy sauce (p<0.05). Consequently, the soy sauce with tea extract and garlic vinegar may enhance the satisfaction of consumer and antimicrobial activity that against V. parahaemolyticus and V. vulnificus, resulting in potential reduction the outbreaks of food borne pathogens.

晋陽姜氏는 우리나라의 대표적인 名門大族으로 역사상 많은 인물을 배출해 각 방면에서 크게 활약해 왔다. 진양강씨는 모두 高句麗 말기의 장군 姜以式을 시조로 삼는다. 강이식의 후손 가운데 고려 후기 姜啓庸은 벼슬이 國子博士였으므로 그 후손들을 博士公派라 일컫는다. 강계용의 손자 姜師瞻은 벼슬이 監察御史였으므로 그 후손들을 御史 公派라고도 한다. 어사공은 공목공의 증조부이다. 恭穆公 姜蓍는 1339년 晋州에서 태어났다. 1357년 成均試에 합격하여, 1362년 출사한 이후 내외의 여러 관직 을 거쳐 門下贊成事에 이르고 晉山君에 봉해졌다. 恭穆公이 가문의 위상을 크게 격상시켰고, 공목공에 이어 그의 형제 와 아들들이 모두 정승의 반열에 들어 일국의 명문이 되었다. 당시 불교가 성행하던 시대에 이미 儒敎思想을 철저히 실천했고, 실 용학문인 農學에도 관심을 가져 農書를 간행해서 보급했다. 두 아들 通亭 姜淮伯, 通溪 姜淮仲이 朝鮮 왕조에 다시 출사하여 가문의 기반을 닦았다. 이후 姜碩德, 姜孟卿, 姜希顔, 姜希孟 등 학문에 있어서나 仕宦에 있 어서 걸출한 인물들이 많이 나와 朝鮮王朝 내내 名門家로서의 위상을 유지했다. 이 가문이 이렇게 장기간 동안 명문가로서의 위상을 유지할 수 있었 던 것은, 好學과 興學의 정신, 孝友와 忠節을 숭상하는 정신, 겸양과 節儉을 중시하는 정신, 實學的 분위기, 禮讓崇尙의 家風, 행정능력의 출중함, 中國文物의 수용정신 등이 原動力이 되었던 것이다.

Sensory evaluation, in vitro antioxidant activities and main compounds of coffee water-extract, coffee liqueur (CL) and coffee-ground liqueur (CGL) were investigated to consider their industrialization. Sensory evaluation showed that all groups of CGL without 25% CGL (3 month) were relatively higher than CL groups. Total phenolic compounds and in vitro antioxidant activities such as 1,1-diphenyl-2picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) were also performed. The group of 35% CGL had higher total phenolic compounds than others, and the result of DPPH radical scavenging activity was similar to that of total phenolic compounds. In addition, 35% CGL is comparable to the FRAP of coffee water extract (CE). Qualitative and quantitative analysis using high-performance liquid chromatography (HPLC) were performed, and chlorogenic acid as a ployphenolic compound and caffeine as a nonpolyphenolic compound were detected in all samples. Moreover, the HPLC analysis showed that CGLs contain a larger amounts of chlorogenic acid (difference of 0.3~10.5%) and also greater amounts of caffeine (difference of 10.0~18.2%) more then CE. Consequently, these results suggest that coffee-ground as coffee by-products could be used as commercially available food substances because of its physiological molecules remained.

This survey was conducted in order to investigate the vascular plants of Mt. Goryeong and Gaemyeong in the DMZ area of Korea. The vascular plants were collected eight times from April 2014 to October 2014 and consisted a total of 450 taxa; 91 families, 279 genera, 391 species, 4 subspecies, 49 varieties and 6 forms. The observed rare plant species as designated by Korea Forest Service were 5 taxa including Tylophora floribunda Miq., Aristolochia contorta Bunge, Viola albida Palib., etc. Endemic plant species were 6 taxa containing Aconitum pseudolaeve Nakai, Clematis brachyura Maxim., Clematis trichotoma Nakai, etc. 33 taxa were listed as specific plant species based on phytogeographical in the investigated area. 35 taxa were recorded as naturalized plants, and their naturalized rate and urban index were noted as 7.7%, and 10.9%, respectively. Of the 450 taxa listed; 186 were edible plants, 173 pasture plants, 149 medicinal plants, 51 omamental plants, 20 timber plants, 13 stain plants, 4 fiber plants, 4 industrial plants and 92 unknown resource plants.

본 연구에서는 우리나라 강수 및 기온과 다양한 기상인자와의 교차상관관계 분석을 통해 대규모 기후변동이 우리나라에 어떠한 영향을 주는지를 분석하였다. 강수 및 기온자료의 경우 앞선 연구인 “경험적 모드분해법을 이용한 기상인자와 우리나라 강수 및 기온의 상관관계 분석 : I. 자료의 분 해 및 특성분석”의 연구결과를 통해 주기성, 경향성에 따라 분해한 강수 및 기온자료의 내재모드함수를 사용하여, 자료의 변동이 심하고 잡음이 포 함된 원 자료를 통한 상관관계 분석보다 좀 더 명확한 상관계수를 계산하였다. 이렇게 분해된 기상자료와 기상인자간의 교차상관관계 분석을 통해 그 시간차와 상관계수를 계산하여, 주기성과 경향성 측면에서 어떠한 상관관계가 있는지를 분석하였다. 그 결과 주기성 측면에서 엘니뇨현상에 의 한 기후변동이 우리나라 기상현상과 밀접한 관련이 있으며, 경향성 측면에서 기후변화로 인한 해수면 온도 증가추세가 우리나라 기상현상과 밀접 한 연관이 있음을 알 수 있었다.

최근 기후변화로 인한 자연재해가 증가하면서 강수 및 기온자료의 시계열에 대한 변동성과 추세를 분석하여 그 변화를 예측하는 연구의 필요성 이 점점 커지고 있다. 하지만 강수나 기온의 경우 복합적인 요소에 의해 변동이 일어나 자료의 변동성이 매우 심하고 너무 많은 요소를 포함하게 되 어 그 특성을 정확히 판단하기가 쉽지 않다. 따라서 자료의 시계열을 분해하게 되면 각 특성을 가진 요소를 추출할 수 있으므로, 정확한 변동 특성을 파악할 수 있다. 본 연구에서는 우리나라 강수 및 기온자료를 경험적 모드분해법(Empirical Mode Decomposition, EMD)을 통해 주기별로 분해 하여 각각의 내재모드함수(Intrinsic Mode Function, IMF)를 추출하였다. 또한, 추출된 내재모드함수의 에너지 밀도를 이용한 유의성 검정을 통 해 원자료로부터 유의미한 자료를 포함하고 있는 내재모드함수를 선별하고, 이들의 주기성, 경향성을 분석하였다.

Bakanae (foolish seedling) disease caused by Gibberella fujikuroi creates serious problems in the foremost rice growing countries. This study was conducted to identify new resistance genetic sources to Bakanae disease. Bioassay showed that 11 varieties including Gwangmyeongbyeo, Hawn, Wonseadaesoo, Erguailai etc. were resistant to bakanae disease among 254 rice germplasm. Mismatch ratio between phenotype on bakanae disease bioassay and allele type of RM9, a SSR marker closely linked the bakanae disease resistant QTL, qBK1, were 38.3%. These results suggest that RM9 might be used for selecting qBK1, but it cannot be used for wide range of rice germplasm. Resistant germplasm in this study might be have resistant genes different from qBK1. The eleven varieties resistant to selected in this study will be used to identify new resistant alleles or genes to improve bakanae disease resistance in rice.

Drought caused by global climate change is one of serious problems for rice cultivation. However, it was little reported the impact of drought on rice cultivation in Korea. In here, to assess impact of drought on rice varieties in Korean climate condition, growth characteristics and yield components of rice were compared on irrigated and partially irrigated rice paddy field. First, we have chosen 11 rice varieties including ‘Saeilmi’ and ‘Shindongjin’ which are widely cultivated in Korea. For partially irrigated rice paddy treatment, we have withheld irrigation from 25 days after transplanting and water supply was totally dependent on rainfall for rice cultivation. When we examined early plant height and tiller number of these varieties on partially irrigated rice paddy were reduced 1.6% to 18.4% and 10.4% to 33.1%, respectively, and these reduction rate were highly correlated with yield loss in our experimental conditions. Among rice yield components, panicle number was decreased 10.5% to 30.1% according to rice varieties and reduced panicle number was highly correlated with yield loss. Grain number per panicle, grain filling rate and 1,000 seeds weight did not have correlation with yield loss of rice varieties. These result means that growth stage, especially the tillering stage, is seriously affected by drought on rice cultivation in Korea. And we suggest that ‘Saeilmi’, ‘Ilmi’ and ‘Ilpum’ are good for rice cultivation on drought prone rice field in Korea.

Seed dormancy is an important adaptive mechanism to protect seeds under the unfavorable environments. Unlike to wild type species, the seed dormancy trait of cultivated crops has been weakened by breeding programs during the domestication period. Weak seed dormancy often causes preharvest sprouting (PHS) problem in many cereal crops that result in significant economic loss. The seed dormancy is a quantitative trait loci (QTL) controlled by multiple genetic and environmental factors. So far, many QTLs for seed dormancy have been identified from rice and wheat as well as in the model plant Arabidopsis. Unveiling of QTL genes and complex mechanisms underlying seed dormancy is accelerated by the rapid progress of crop genomics. In the present study, we reviewed current status of research progress on the seed dormancy QTLs and correlated genes in Arabidopsis and cereal crops.