본 연구는 산업화 유망 식물인 회화나무의 이차대사산물 대량생산용 캘러스 확보에 필요한 기초조건을 확인하고자 수행하였다. 기내 배양한 회화나무 하배축을 2.0 mg·L-1 NAA + 0.1 mg·L-1 TDZ 배지에서 배양하였을 때 유백색의 부드러운 캘러스가 형성되었고, 캘러스 형성률 100±0.0%, 캘러스 형성에 걸리는 기간 7.1±0.03일, 캘러스 무게 249.8±12.73 mg으로 가장 효과적임을 확인하였다. 또한, 하배축에서 유도한 캘러스는 백색광 조건의 1.0 mg·L-1 IBA + 0.1 mg·L-1 TDZ 처리 배지에서 배양하였을 때 연노란색의 단단한 캘러스가 다량으로 형성되었고, 캘러스 생체중 186±5.4 mg, 면적 40.6±1.6 mm2으로 가장 무겁고, 크기가 우수한 것을 확인하였다. 기내 배양한 캘러스 소재 추출물의 추출수율은 50% 에탄올 추출 시 59%, 물 추출 시 54%로 확인하였고, 추출물 내 유효성분으로 마키아인 함량은 1.68 mg·g-1으로 검출되었다. 본 연구의 결과는 산업화 식물로써 유망있는 수종인 회화나무의 기내 대량증식과 이차대사산물 생산성을 향상할 수 있는 위한 기초자료로 활용할 수 있을 것이다.

우리나라 여러 해양환경 지역으로부터 확보한 370주의 해양세균, 균류, 미세조류로부터 기초생 리활성(항산화, 항염, 항균, 항암, 항바이러스)을 조사하여 채집지, 분리원, 종(種) 수준에서의 활성결과를 비교하였다. 해양세균의 경우, 일반적으로 유용성이 많이 알려진 Streptomyces 속 과 Bacillus 속에 속하는 균주들이 두드러진 강한 효능이 관찰되었고, 주로 해양퇴적물로부터 유용한 자원을 분리할 수 있었다. 해양균류와 미세조류의 경우에도 종 특이적으로 활성이 강 하게 나타나는 결과를 확인할 수 있었고, 효능 특이적으로 활성을 보이는 결과도 얻을 수 있었 다. 이러한 결과를 바탕으로 추후 특정질병에 선택적으로 효능을 보이는 화학물질 연구 또는 자원 기반 연구 수행 시 유용성을 전제로 한 자원 확보 전략 수립과 산업 활성화를 위한 전 략소재로 우선적 접근이 용이할 수 있는 연구결과라 생각된다. 또한, 이들 결과를 해양바이오 뱅크를 통한 분양소재로 활용함으로써 학계, 산업계에서 활용하여 해양바이오산업 활성화에 좀 더 빠른 접근을 도울 수 있다고 생각한다.

본고는 단국대학교 부설 한문교육연구소에서 개발 중인 특수 어휘 매칭 툴을 구동시켜 어휘 데이터의 문제점과 개선 방향을 논의하기 위해 작성되었다. 한문교육연구소 개발 어휘 매칭 툴은 최종적으로 한문 고전 텍스트를 토크나이징(Tokenizing) 하기 위한 것이며, 특수 어휘의 매칭은 전체 텍스트를 토크나이징을 하기 위한 첫 단계라고 할 수 있다. 이 어휘 매칭 툴 실행 결과를 MARKUS 자동 마크업과 비교함으로써 매칭 툴과 그 데이터의 장단점을 분석하고, 이 과정에 발견된 문제점에 대해 보완할 수 있는 방향을 제시하였다. 한문교육연구소 어휘 매칭 툴은 한문고전에 특화된 도구로서 중요한 역할을 할 수 있으며, 앞으로 한문 고전의 토크나이징 에도 기여할 것으로 기대된다. 하지만 현재 상태에서는 여러 가지 보완이 필요하다. 우선, 한국 고유의 지명과 인명 데이터를 추가할 필요가 있다. 현재 데이터는 주로 중국의 어휘에 집중되어 있어 한국 고유 어휘가 부족한 상황이다. 추가 어휘데이터를 구축함으로써 해결할 수 있을 것으 로 보인다. 또 별칭의 매칭 문제 등을 해결할 필요가 있다.

Changes in contents of free sugars, amino acids, and fatty acids of legumes were analyzed for each phase of in vitro digestion. In addition, contents of resistant starch in raw and digested pulses were compared. Soybeans, kidney beans, cowpeas, and chickpeas were analyzed. An in vitro digestion model was used to analyze contents of nutrients using LC-MS and GC-MS. Stachyose in kidneybean, cowpea, and chickpea increased as the digestion phase progressed. In four types of legumes, raffinose slightly decreased or showed no significant difference between the Oral phase and the BBMV phase. Content of glucose, a monosaccharide, increased during the BBMV phase. During the digestion phase, levels of free amino acids and free fatty acids also increased. Content of resistant starch was reduced compared to that in the raw material. It was 0.01g/100 g food in soybean, 1.06 g/100 g food in red kidney bean, 0.77g/ 100g food in cowpea, and 0.76 g/100 g food in chickpea. It was confirmed that nutrients in the in vitro digestion model were liberated at each digestion phase with changes in the content of resistant starch. These results are expected to be used as fundamental data for obtaining bioavailability of nutrients.

The purpose of this study was to analyse factors related to Home Meal Replacement (HMR) use among university students and to determine the dietary quality according to its consumption. A survey on the consumption of HMR and Nutrition Quotient (NQ) was conducted from September to November 2021. The study included 232 university students (88 males, 144 females) from Chungcheong. The proportion of consumption at least once a week was 71.55% for ready-to-eat foods, 55.60% for ready-to-cook foods, 40.95% for fresh-cut products, and 21.12% for meal-kits. The preference ratings were as follows: ready-to-eat foods, 3.77 out of 5 points, meal-kits, 3.53 points, fresh-cut products, 3.52 points, and ready-to-cook foods, 3.45 points. In terms of satisfaction, convenience (4.06 out of 5 points), taste (3.71 points), variety (3.67 points), and food hygiene (3.62 points) were rated the highest. The scores in the moderation NQ were significantly lower in the groups that consumed ready-to-eat foods (p=0.0002), ready-to-cook foods (p=0.0002), and meal-kits (p=0.0068) at least once a week compared to the groups that consumed them less than once a week. In conclusion, the results will serve as basic data for nutrition education for proper consumption of HMR among university students.

This study was conducted to obtain basic data on domestic wild Lanopila nipponica K specimens collected in Jeollanam-do from 2015 to 2017 to investigate the characteristics of fruit bodies and analyze their nutritional components. L. nipponica K. was mainly found near hills, such as in persimmon fields around hills, on mountain trails, and in bamboo fields. L. nipponica K. occurred from July to mid-October, especially after the rainy season or a typhoon, when the air humidity was increased. Rainfall occurred for 8–15 days during the 20 days before the collection date, and the average temperature was ≥ 23oC. The size of the fruit body was 12.3–28 cm, and the weight varied depending on the size; however, the fruit body was estimated to grow overnight, reaching the size observed at its discovery. As aging progressed, the surface of the fruit body cracked, and it turned dark after 6 days. Spores were lump-shaped when the surface was cut with a knife. When the soil on the surface of the site where the fruit body was located was scratched, a white thread-shaped mycelium expanded. At the time of collection, the flesh of the fruit body was white, but it turned brown and fluffy as maturity progressed. On the sixth day after the mushroom was generated, it produced a pungent odor and became friable, resembling a black cotton ball. The surface of the fruit body was white and had a circular or elliptical shape. Crude protein content tended to be high at 59% of the dry weight. The antioxidant activity, determined using the DPPH radical scavenging analysis method, was 167 vitamin C equivalents/100 g. The spores were brown, approximately 6 μm in size, with protrusions on their surface.

Tempeh is a traditional Indonesian food that is fermented soybeans with Rhizopus spp. and contains high-quality protein. In this study, tempeh and yeast were set as independent variables following the central composite design to optimize the nutritional quality and sensory characteristics of high-protein loaf bread added with tempeh. The optimal mixing ratio was calculated through the response surface methodology. The optimal mixing ratio of tempehadded loaf bread was 11.27 g of tempeh and 5.46 g of yeast. A comparative analysis of the quality characteristics between the tempeh-added loaf bread and the control group produced by the optimal recipe showed that tempehadded loaf bread contained higher protein content, lower carbohydrates and fats, and lower calories. Among amino acids, essential amino acids such as aspartic acid, threonine, glycine, alanine, lysine, and histidine were significantly higher in tempeh-added loaf bread. Among fatty acids, butyric acid, capric acid, myristic acid, palmitic acid, palmitoleic acid, and heptadecanoic acid were significantly higher in tempeh-added loaf bread, while lauric acid, linoleic acid, and linolenic acid were significantly lower. This study confirmed the suitability of tempeh as an ingredient for protein-enhanced loaf bread and the potential possibility of it being utilized in bakeries.

Melons, a rich source of vitamins and fibers, are commonly grown in the soil. Hydroponic cultivation could improve yield and quality of melon and selection of substrate volume and the kind of substrates is important for hydroponic cultivation of melons. This study investigated the effect on melon growth according to volume of granular rockwool and substrates of coir and rockwool slab. ‘Geumsegye’ melon (Cucumis melo L. cv. Geumsegye) was cultivated hydroponically according to volume of granular rockwool to 1.0, 1.5, 2.0, 3.0, and 4.0 L, and was also cultivated using coir and rockwool slabs. Logistic model was applied to estimate the growth characteristics of melons such as plant height, leaf length, leaf width, and the characteristics of fruit. The growth characteristics of melons were significantly increased at 4.0 L compared to those grown of 1.0 L volume of on granular rockwool, and the results were the highest in coir and rockwool slabs. Melons grown in rockwool slabs showed the largest fruit fresh weight, fruit length, and fruit width. During hydroponic cultivation, growth characteristics of melon appropriate at the 4.0 L volume of granular rockwool, and the highest at coir and rockwool slabs. This study provides a basis for understanding the effect of root zone environment to the growth characteristics and fruit quality of non-netted melon.

Porphyromonas gingivalis, a major pathogen of chronic periodontitis, colonizes in subgingival crevice and affects surrounding oral tissues, especially in periodontitis patients. Oral cancer mainly occurs in old-aged persons, and are exposed to the P. gingivalis, released from periodontitis, one of the most common inflammatory disease of oral cavity. Thus oral cancer cells may be infected with P. gingivalis, and its biologic behavior are autologously and/or heterogeneously modulated by altering gene expression. Exosomes which are derived from cells contain not only coding genes but also non-coding RNAs such as long non-coding RNAs, miRNA, and piRNAs. Here, to investigate the effect of P. gingivalis on oral cancer cells and to gain insight into the crosstalk between inflammatory signal from tumor microenvironment and oral cancer, we observed miRNA profiles of exosomes from P. gingivalis–infected oral cancer cells. Upregulation of 6 miRNAs, miR-203-3p, miR-6516-3p, miR-483-5p, miR-1275, miR-8485, and miR-19a-3p, were observed whereas 14 miRNAs including let-7a-3p, miR-106a-5p were downregulated. In addition, KEGG pathway analysis using the upregulated- and downregulated- miRNAs showed association with cell adhesion molecules pathway and ECM-receptor interaction pathway, respectively. These findings suggest that P. gingivalis could modulate biologic behavior of oral cancer cells through changes of exosomal miRNAs.