본 연구는 서울·경기 소재 65세 이상 노인 463명을 대상으로 신체활동 참여에 따른 체력요 인과 인지기능, 일상생활수행능력, 도구적 일상생활수행능력 및 심리적 변인에 대한 차이를 분석하였다. 수집 된 자료는 SPSS 25.0 통계프로그램을 이용하여 빈도분석, 신뢰도 분석, 독립 t-검증과 일원분산분 석 그리고 피어슨의 상관관계분석을 실시하였으며 모든 통계적 유의수준은 p<.05로 설정하였다. 그 결과 첫째, 노인의 신체활동 참여유무에 따라 체력, 인지기능, 일상생활수행능력, 도구적일상생활수행능력 및 심리적 변인인 우울과 삶의 질에 통계적으로 유의한 차이가 나타났으며, 신체활동에 참여하는 노인의 참여빈도에 따라 체력, 인지기능, ADL, IADL, 우울 및 삶의 질에 통계적 유의한 차이가 나타났다. 신체활동에 참여하는 노인의 참여시간에 따라 체력에서 통계적 유의한 차이가 나타났다. 둘째, 체력과 인지기능, ADL, IADL, 우울 및 삶의 질의 상관관계를 살펴본 결과 대부분의 요인에서 상관관계가 나타났다. 이러한 연구결과를 바탕으로 노인의 성공적인 노화와 자립적인 노후생활을 위해 신체활동을 포함한 체계적인 중재 전략의 개발이 필요하다.

The present study was designed to determine the effect of barn or cycle of grazing on changes of biochemical metabolites in prepartum and changes of milk composition in postpartum of dairy cows. For this purpose, a total of sixteen 25 months old Holstein primiparous dairy cows were allocated in two groups (n=8) with an average body weight of 571.61 ± 35.30 kg (Barn) and 578.10 ± 39.20 kg (Grazing). The study was conducted from June 2018 to October 2018. Results revealed that barn raised dairy cows had a higher increase in their serum albumin and calcium level on day 14 prepartum. However, the level of palmitic acid, saturated fatty acid increased significantly, and the level of fat, oleic acid, γ-linoleic acid, arachidonic acid and unsaturated fatty acids decreased significantly in barn raised dairy cow’s milk on day 14 postpartum. There were no significant differences observed with respect to all other biochemical metabolites, fatty acids and minerals between barn raised and cycle grazing dairy cows during prepartum and postpartum. Our study results could serve to a better understanding of barn raised cow with respect to changes of biochemical metabolites in prepartum and changes of milk composition, fatty acids and minerals content in grazing dairy cows in postpartum for estimating their physiological status.

An experiment was carried out to assess the effect of feed selenium-lysine (Se-Lys) supplementation on milk compositions and serum biochemical indices in Saanen dairy goats in Korea. A total of twelve 36 months old Saanen lactating dairy goats (47±6.21 kg) fed the similar dry matter intake twice a day at 2% of BW (DMI) (10.9% moisture of concentrate and 19% moisture of roughage), milk yield (2.5 kg/d) and parity (2) were randomly selected and subjected for the present study, divided into two groups with six goats in each group. The goats in the control group received rice hulls (10 g/ day) only, and did not receive Se-Lys; goats in the treatment group were fed 0.06 g of Se-Lys with 10 g of rice hulls every day before feeding roughage for six weeks. The milk sample was collected every week, and its compositions were analyzed. The results of the present study showed that there is no significantly increased milk production in Se-Lys treated group goats when compared with control group goats. But, Se-Lys treatment significantly increased the milk protein content (3.98±0.16%), fat (3.72±0.27%), lactose (4.07±0.14%), total solids (12.51±0.28%) and urea (14.42±1.45 mg/dl) content as compared to the control group goats (p<0.05). The somatic cell counts (207,740±28.81 cells/ml) were significantly lower in the Se-Lys treated group than in the control group (p<0.05). Also, the results of the current study showed that supplementation of Se-Lys were significantly decreased the blood biochemical indices of IL-6 (34.34±6.04 pg/ml), TNT-α (0.56±0.22 ng/ml), MDA (5.07±1.03 ng/ml), GPx-1 (9.07±5.17 ng/ml), sCD4 (2.64±1.02 ng/ml) and sCD8 (5.08±2.08 ng/ml) level when compared with without addition of Se-Lys group dairy goats (p<0.05). On the other hand, the selenoprotein P (1,580.18±127.62 ng/ml) level was significantly higher in Se-Lys supplemented group than in the control group (p<0.05). Based on the study results, it was concluded that feed Se-Lys supplementation may improve milk yield with positively improved protein, fat, lactose, total solids, urea content, and biochemical indices without negative effects on milk production traits.

The light treatments were composed of red, blue, white, far-red, red+far-red, red+blue, red+blue+white LEDs and duty ratio(%) of mixed light red+blue (100, 95, 90, 85, 80, 75), red+blue+white (100, 85, 70). The following results were obtained in different LED light sources treatment: Red leaf lettuce’s leaf number were the most under white LED. Leaf size was the highest under red+blue LEDs and shoot length was the longest under red+far-red LEDs. Shoot were the heaviest under red LED. Blue leaf lettuce’s leaf number and shoot length were high under all light treatment except far-red LED. Each vertical and width length of leaves were the longest under red+blue LEDs, white LED. Leaf number of red leaf lettuce were more in 85%-100% duty ratio than in 75, 80% duty ratio and leaf size was highest in 100% duty ratio under the mixed light red+blue LEDs. Shoot length was the highest in 90% duty ratio. Blue leaf lettuce’s leaf number and shoot length showed no difference in LED light treatment. Leaf size was the highest in each 100, 95%. Shoot and root biomass were highest in 95%. Shoot length of red leaf lettuce was the highest in 70% duty ratio nunder the mixed light of red+blue+white. The others showed no difference in duty ratio. Blue leaf lettuce’s leaf number, shoot length and biomass were the highest in 85% duty ratio. Thus, we can cultivate stably without reference to external factors, if we use appropriate light sources and light quality in closed-type plant factory.

Oxidative stress has been reported to be one of causes of neuritis. This study examined antioxidative activities of methanol extracts of six amphibian species known to be medicinal animals (Rana catesbeiana, R. coreana, R. rugosa, R. dybowskii, R. nigromaculata, and Hyla japonica) and investigated their effects of inhibiting nitric oxide (NO) production and cytotoxicity on the murine macrophage RAW264.7 cells. As inflammation is closely associated with reactive oxygen species, assays on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, xanthine oxidase inhibitory activity, superoxide anion radical scavenging activity and NO scavenging activity of the extracts of the six species were performed to investigate their antioxidative activity. The results obtained were as follows; All extracts showed antioxidative activity, and the activity of R. dybowskii was the highest in comparison among those. Anti-inflammatory effects of the extracts were also examined, the five extracts except that of R. rugosa did not show cytotoxicity for RAW264.7 cells at the maximal concentration (1,000 μg mL-1). Selectivity index, meaning NO scavenging activity compared to cytotoxicity, showed the highest level in the extract of R. dybowskii. These results will be very useful basic data for future studies on prevention and treatment of human diseases to understand the biological roles of amphibian extracts throughout the antioxidative or anti-inflammatory pathways.

This study was conducted to examine the effect of dietary n-3/n-6 fatty acid (FA) ratio on in vitro dry matter digestibility (IVDMD), fermentation indices and FA profile. Rice bran was mixed with oil sources (cotton seed oil and linseed oil) to make the diets at 0.02, 0.29 and 0.61 of dietary n-3/n-6 FA ratio. These diets (0.5g) were placed into the incubation bottles with 40 ml of anaerobic culture medium, which contained rumen fluid and Van Soest medium at 1:2 ratio. Five replicates of each diet and two blanks were incubated at 39℃ for 48 hours. After incubation, the incubated contents were centrifuged. The residues were freeze-dried for DMD and FA analyses. The supernatant was used for pH, NH3-N and volatile fatty acid analyses. The concentrations of lactate (p<0.001) and iso-valerate (p<0.001) decreased linearly with increasing dietary n-3/n-6 FA ratio, but acetate concentration (p=0.056) and the ratio of acetate to propionate (p=0.005) was increased linearly. The concentrations of n-3, n-6 FA and the ratio of n-3/n-6 FA in residues increased (p<0.001) linearly with increasing dietary n-3/n-6 FA ratio, but C18:1n-9 FA concentration was decreased (p<0.001) linearly. With these results, it could affect fermentation characteristics and FA profile of rumen content by dietary n-3/n-6 FA ratio.

본 시험은 포도부산물의 첨가가 젖소의 산유량, 유성분 및 혈액 성상에 미치는 영향을 조사하기 위하여 실시하였다. 비유중기 젖소 8두(평균 월령: 89.8, 평균 유기: 164.4, 평균 산차: 2.8산)를 공시하였으며, 포도부산물을 첨가하지 않은 대조구와 1일 1 kg을 첨가한 시험구로 두 처리구를 설정하여 3주간 급여하였다. 유지방, 유단백, 유당 및 총 고형물 함량은 처리간에 차이가 없었다. 그러나 산유량은 포도부산물 을 첨가한 처리구가 23.13 kg/d으로 대조구의 21.16 kg/d에 비해 높은 반면(p<0.05), 체세포 수는 대조구가 포도부산물 처리구에 비해 높았다(p<0.05). 혈중 glucose, alanine aminotransferase, total protein 및 blood urea nitrogen 함량은 처리구간 차이가 없었으나, AST와 총콜레스테롤 함량은 대조구 (123.75 IU/L, 200.82 mg/dl)가 포도부산물 처리구(95.34 IU/L, 180.63 mg/dl)에 비해 높았다(p<0.05). 이상의 결과에서, 다양한 생리활성을 가진 포도부산물은 젖소를 위한 기능성 사료로 이용 가능성이 높을 것으로 사료된다.

The objective of this study was to compare the accuracy analysis and the effect of field application of a newly developed automatic heat detector in dairy cows. From 2009 to 2010, we used 48 Holstein cows (mounting cows : 38 heads, standing cows : 10 heads) raised in experimental barn of National Livestock Research Institute (RDA) for the accuracy analysis of automatic heat detector, and 14 Holstein cows raised in three commercial dairy farms of Cheonan and Pochun area for comparison of the effect of field application. The accuracy of response in cows attached with automatic heat detector was 86.8% (33/38) displayed on board when mounting activity observed, and 100% (10/10) when standing activity observed, and on average, 90.0% (43/48) displayed on board. The accuracy of automatic heat detector in on-farm test was 85.7% (12/14), and conception rate was 75.0% (9/12).

The objective of this study was to investigate the relationship between estrous expression, body condition score (BCS), blood urea nitrogen (BUN) and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Sixty, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2α was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 μg GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.25 than for cows with BCS above 2.25. There was 50.0% of rate of mounting in cows with BCS below 2.25 whereas the rate of mounting was markedly increased in cows with BCS above 2.25 (94.1% and 89.5% for BCS 2.25～2.75 and BCS above 2.75 cows, respectively). Cows with BCS ＜2.25, 2.25～2.75 and ≥2.75 had number of transferable embryos of 4.5±0.7, 5.9±1.8 and 5.6±2.3 respectively.

The objective of this study was to investigate the relationship between concentration of urea nitrogen, glucose, cholesterol and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Fifty five, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2α was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100μg GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. Cows with BUN ＜10, 11～18 and ≥19 mg/dl had number of transferable embryos of 4.3±1.3, 5.8±1.8 and 4.7±2.1 respectively. The mean numbers of total ova from < 10 and 10≤ of corpora lutea(CL) was 8.9 and 14.3, respectively. The number of transferable embryos differed between < 10 and 10≤ CL was 4.8 and 5.6, respectively.

This study is conducted to screen compounds affecting ruminal fermentation under in vitro incubation. Saponin, chitosan, metformin, zinc acetate, zinc chloride, zinc oxide, zinc sulfate, ascorbic acid, oil-coated ascorbic acid, nicotinic acid, eastern herb cocktail, charcoal extract and garlic sources (lyophilized and extract) were added to rumen culture fluid at 1.25% of substrate (100% timothy) volume at 24 h incubation. pH, total gas, ammonia, VFAs were measured. Ascorbic acid increased total gas production indicating fermentation level. All zinc compounds significantly decreased (p<0.05) although zinc sulfate increased propionate of volatile fatty acid (p<0.05). In following experiment, ascorbic acid, oil-coated ascorbic acid, garlic lyophilized, herb cocktail and zinc sulfate were added to rumen culture fluid at 2.5% of substrate with 3, 6, 12 and 24 h incubation. Zinc sulfate decreased both ruminal fermentation and VFAs production but ascorbic acid enhanced total gas production. Ascorbic acid increased fermentation regardless of supplement concentration although excessive zinc sulfate decreased fermentation. These results suggest that optimal level of trace compounds might affect ruminal fermentation in ruminant.

본 연구는 홀스타인, 한국재래산양 및 한우유로부터 glycomacropeptide(GMP)를 분리하였으며, 각 GMP의 trypsin 가수분해물의 혈소판응집 억제 효과를 in vitro 상에서 알아보았다. 홀스타인, 한국재래산양 및 한우의 GMP는 분자량이 모두 약 20 KDa이었으며, sialic acid 함량은 각각 36.86±2.36, 37.98±1.27 및 31.19±1.87㎍/mg이었다. 또한 모든 개체의 GMP에서 tyrosine이 검출되었다. 홀스타인, 한국재래산양 및 한우 GMP의 trypsin 가수분해물에 의한 혈소판 응집 억제율은 반응 30초에 4.02, 5.51 및 12.77%로 각각 나타나 시간이 경과할수록 감소하는 경향을 보였다. 혈소판의 현미경 관찰 결과 가수분해물 첨가 후 혈소판 수가 증가하였으나, 첨가 후 30초가 경과한 시점부터 혈소판 수가 감소하기 시작하여 120초 후에는 관찰 할 수 없었다. 본 실험 결과 bovine 및 caprine GMP의 trypsin 가수분해물에서 혈소판 응집을 억제할 수 있는 small peptide가 있는 것으로 생각되며, 향후 이러한 연구는 심근경색증 및 뇌혈전증을 예방할 수 있는 생리활성 물질로 이용될 수 있을 것이라 생각된다.

목적: 본 연구는 타이밍, 리듬, 동기화 등의 기능들을 훈련시키고 향상시킬 수 있는 방법으로 Interactive Metronome(IM) 훈련을 설명하고 IM 훈련 효과에 대한 신경생리학적 기전을 근거로 제공하여 다양한 연구영역에 서의 접근과 새로운 연구 문제를 도출하고 탐색하는데 목적이 있다. 방법: 국내⋅외 학술지에서 발간된 논문들 중 IM 훈련을 적용한 논문을 검색하였다. 국내 학술지 web 검색엔진은 학술연구정보서비스, 한국학술정보서비스, 디비피아를 이용하였으며 국외 학술지는 SCOPUS, PubMed, Google scholar를 이용하였다. 검색 주제어는 ‘interactive metronome’, ‘metronome training’ 그리고 ‘synchronized metronome’을 검색하였다. 결과: IM 훈련 효 과에 대한 3가지 신경생리학적 기전 중 첫 번째는 뇌 시계와 시간적 정보 처리로서 IM 훈련을 통해 주요 뇌 영 역 일반 신경인지 메커니즘에 영향을 줄 수 있다. 둘째, 뇌 네트워크 커뮤니케이션과 동기화로 반복적으로 수행 되는 IM 훈련은 뇌신경 네트워크와 뇌 영역 간의 연결성 및 효율적인 커뮤니케이션이 촉진된다. 셋째는 주의 조 절 시스템 기능의 향상이다. IM 훈련은 내⋅외부로부터 발생되는 방해 요소에 대한 통제력을 높일 수 있으며 고 도의 사고와 주의집중을 할 수 있도록 한다. 결론: IM 훈련은 장애 및 질환 영역과 스포츠 및 운동행동 영역에 서 연구가 진행되어 왔으며, 타이밍, 리듬, 동기화 등의 기능들을 향상시킨다. 또한, IM 훈련은 선행연구들을 통 해 뇌신경 가소성이 일어나는 것을 입증하고 있다.

목적: 본 연구는 Interactive Metronome(IM)훈련이 농구 패스 타이밍 정확성과 일관성에 미치는 영향을 분석하고 농구 패스 타이밍 정확성과 일관성을 향상시킬 수 있는가를 검증하는데 그 목적이 있다. 방법: 연구 참여자는 비숙련자 20명을 대상으로 IM훈련을 적용한 실험집단 10명, 통제집단 10명으로 할당하였다. 실험 과제는 불빛 자극 3가지 이동속도 조건(2㎧, 4㎧, 8㎧)에 대해 불빛의 도착지점(표적)과 참여자가 패스한 볼이 시·공간적으로 일치되도록 하는 과제를 20회씩 총 60회를 시행하여 사전검사를 실시하였다. 실험집단에게는 6주간 12회 걸쳐 IM 훈련을 적용하였으며 훈련 종료 후, 사후검사를 실시하였다. 패스 타이밍 수행력은 시각 타이밍 과제의 절대오차와 가변오차를 통해 분석되었다. 산출된 자료는 시기에 반복이 있는 이원분산분석(Two-way ANOVA with repeated measures on time)을 통해 분석되었다. 훈련의 효과가 지속적으로 유지되는지를 확인하기 위해 파지검사를 실시하였다. 결과: IM훈련을 적용한 실험집단은 농구 패스 타이밍 정확성과 일관성이 향상된 것으로 나타났으며, 타이밍 수행능력이 지속적으로 유지되는 것으로 나타났다.

본 연구는 홍시라떼 분말화의 최적조건 설정을 위하여 반응표면분석법(RSM)을 이용하였으며, 독립변수는 inlet temperature(X1), air flow rate(X2), 시료의 공급속도인 feed flow rate(X3)로 설정하고 종속변수로는 회수량(Y1), 수분흡 수지수(Y2), total phenolic compounds(Y3)로 설정하였다. 각 종속변수에 따른 회귀식은 모두 p<0.001 수준에서 유의성이 인정되었다. 홍시라떼의 경우 과당이 풍부하여 분말의 부착성이 높기 때문에 유리전이 온도와 입자의 표면 온도 차이가 낮을수록 회수량은 높아졌으며, 분말의 결정성과 응집성은 증가하여 수분흡수지수는 낮아졌다. 또한 낮은 inlet temperature(X1)와 높은 feed flow rate(X3)는 입자의 표면 온도와 열의 접촉 시간을 모두 낮추어 total phenolic compounds의 손실을 최소화 하였다. 각 독립변수와 종속변수의 영향을 나타내는 반응표면그래프를 이용하여 최적 분말화 조건을 예측한 결과 inlet temperature(X1) 90℃, air flow rate(X2) 53 mL/min, feed flow rate(X3) 17.00 mL/min로 결정되었다.

This study aimed to estimate the effects of replacing Mushroom By-Product (MBP) with Tofu By-Product (TBP) on the chemical composition, microbes, and rumen fermentation indices of Fermented Diets (FDs). The basal diet was formulated using MBP, TBP, rice bran, molasses, and inoculants. The MBP in the basal diet was replaced with TBP at 0, 5, and 10% on Dry Matter (DM) basis for the experimental diets. The experimental diets were fermented at 39°C for 144 h. Chemical composition, pH, microbes, and rumen fermentation indices of the FDs were analyzed. With increasing TBP replacement, crude protein content of FDs increased (L, P < 0.001), whereas crude ash content decreased (L, P = 0.002). Lactic acid bacteria and Bacillus subtilis contents in the TBP-replaced FDs were higher than those in the control (P < 0.05), whereas pH level and mold count were lower (P < 0.05). With increasing TBP replacement, in vitro rumen digestibility of DM (L, P = 0.053) and neutral detergent fiber (L, P = 0.024) increased, wheres rumen pH changed (P = 0.026) quadratically. Rumen total volatile fatty acid (L, P = 0.001) and iso-butyrate contents (Q, P = 0.003) increased with increasing TBP replacement. In conclusion, this study indicates that the replacement of MBP with TBP could improve the quality of FD.