This study was performed to investigate the effect of flonicamid and thiamethoxam treated at sublethal concentration (LC10, LC30) on development period, adult longevity and fecundity and the feeding behaviour of Myzus persicae adult. Developmental period of M. Persicae nymph took 5.9 days in LC10, and 6.1 days in LC30 in both insecticides, comparing with control (5.7 days), it showed longer than those of the control, but there was no significance. Adult longevity treated at LC10 and LC30 of flonicamid was showed 13.2 and 13.7 days, respectively, and LC10 of thiamethoxam was examined as 14.7 days, it showed longer than control of 11.6 days. Mean daily fecundity exhibited higher in LC10 (3.1) and LC30 (3.1) of flonicamid than that of control (2.5), but thiamethoxam are not. Total fecundity exhibited higher in LC10 (41.8) and LC30 (43.0) of flonicamid, in LC10 (42.1) of thiamethoxam than that of control (29.5). Feeding behavior was examined using EPG (electrical penetration graph). EPG data indicated that flonicamid and thiamethoxam increased the duration of non-probing periods and decreased the duration of phloem ingestion.

Aphids feed on host plants by penetrating the stems or leaves with stylets. The feeding behavior of aphids consists of probing, penetration, salivation, and sap ingestion. To assess the effects of sound on feeding behavior, we monitored the stylet activity of the green peach aphid, Myzus persicae (Sulzer), using electrical penetration graph (EPG). The use of EPG was critical for determining the stage, frequency, and duration of feeding in aphids. We played back three acoustic stimuli of sine waves with frequencies of 100, 1000, and 5000 Hz to adult aphids. In the sound treatment group, the frequencies of probing, penetration, and salivation increased, whereas the duration of sap ingestion decreased. The results of EPG revealed that the acoustic stimuli may restrict aphid feeding by inhibiting sap ingestion.

The green peach aphid, Myzus persicae (Sulzer), is one of the most important insect pests in the world. We have explored the possibility of using sounds as a control agent in this species. Sine waves with four different frequencies (100, 500, 1000, 5000 Hz) were used as acoustic stimuli in the playback experiments. As a behavioral bioassay, we recorded honey dew production (HDP), wagging, and walking. HDP occurs regularly at each stage of nymphal and adult periods, and the rate of HDP may indicate a degree of food consumption. Aphids conduct wagging to determine a host plant or to find a feeding site. Walking may be regarded as dispersal. All acoustic stimuli significantly reduced the rates of HDP and increased the rates of wagging. There were no significant effect of acoustic stimuli on walking. Based on the bioassay, the acoustic stimulus with 5000 Hz seemed to be the most effective on inhibiting feeding behavior of this aphid species at an individual level.

Three insect pests internally feed pome fruits in Korea. These include oriental fruit moth (Grapholita molesta), plum fruit moth (Grapholita dimorpha) and peach fruit moth (Carposina sasakii). Molecular markers discriminating these three species were developed using PCR-RFLP technique. Mitochondrial (mt) genomes were analyzed to locate polymorphic loci. Six mtDNA regions (CO-Ⅰ, CO-Ⅱ, CB, 16SrRNA-12SrRNA, ND3, ND4) of G. dimorpha were cloned and sequenced. These six sequences of G. dimorpha were aligned with those of C. sasakii and G. molesta to determine polymorphic restriction sites. Predicted PCR-RFLP markers were confirmed with known insect samples. With the validated PCR-RFLP markers, field male adults collected in traps baited with rubber sept lures impregnated with different ratios of major sex pheromone components of G. molesta were analyzed.

본 연구는 사일리지 사료를 급여하였을 때 돼지 품종과 사일리지 급여 유무에 따른 등심의 품질 특성에 미치 는 영향을 평가하기 위해 실시하였다. 돼지품종은 가고시마 와타나베 흑돼지 (B), 삼원교잡종[D (Landrace× Yorkshire)× Duroc], 미국산 버크셔종(A) 등에 대해 각각 시험구와 대조구 20두씩 총120두를 공시하였으며, 개시체중은 평균 69.3± 3 kg, 시험기간은 평균 66.7± 9.3일, 출하일령은 200〜210일이었다. 사일리지를 급여하지 않은 대조구 (C)에 비해 급여한 처리구 (T)가 조단백질 함량은 많았고, 육색은 밝았으며, 신선육 관 능평가 시 외관, 육색, 상강도 및 전체적 기호도에서 높은 점수를 보였고, stearic산과 oleic산은 낮고, linoleic산, arachidonic산, 필수지방산 및 필수지방산/불포화지방산 비율은 높게 나타났다. 사일리지 급여 시 단맛 관련 아미노산 함량은 높은 장점을 지닌 반면 육색의 적색도는 낮고, 가열감량과 육즙감량은 많아 다즙성이 낮았으며, 육의 탄력성이 낮고 지방의 밝기는 어둡고 황색도가 높은 단점을 보였다.

본 연구는 수경재배시 산호수 생육과 개화를 증진시키기 위한 적절한 질소농도 및 공급시기를 구명하기 위해 수행하였다. 질소농도가 120, 150, 180, 210mg·L-1로 조성된 양액을 1년생과 2년생 Ardisia pusilla에 휴면기(10월~3월) 제외한 기간 급액(ED; except dormancy), 생육 전기간 급액(TG; total growth)으로 나누어 처리하였다. 묘령 및 급액기간에 따른 생육을 비교한 결과 1년생 묘는 급액기간에 관계없이 질소농도 150mg·L-1 이상 처리구에서 우수하였다. 2년생묘는 TG기간 동안 급액한 처리구가 ED기간을 제외하고 급액한 처리구들보다 대체적으로의 생육이 훨씬 우수하였으며, 특히 TG-150mg·L-1의 생육이 가장 좋았다. 개화 후 엽의 무기성분 함량을 측정한 결과 묘령에 관계없이 전체적으로 양액내 질소농도가 증가할수록 T-N의 함량은 높아지고 K, Ca, Mg, P의 함량은 약간 낮아지거나 차이가 거의 없었다. 그러나, 1년생 ED-210 처리구는 모든 처리구 중 Ca와 Mg 함량은 가장 높은 반면 K와 P의 함량은 가장 낮았으며 개화수도 적었다. 즉, 질소농도가 210mg·L-1 이상으로 조성되면 오히려 생육이나 개화수가 감소되는 것으로 보아 1년생 산호수는 생장 및 개화를 위해 TG-150으로 처리하고, 2년생 묘는 TG-180이 수경재배시 적정한 급액시기 및 질소농도로 판단된다.

This study was carried out to elucidate changes in milk production, milk components and milk product properties as influenced by organic and conventional farming system. Milk production in transient organic feeding was lower by 7.6% than that in conventional feeding and also milk fat, milk protein content, solids not fat and somatic cell count were lower than conventional farming system. But, milk lactose content in transient organic feeding was higher than that in conventional feeding. Ca and Fe content in milk in organic feeding tended to be higher compared to conventional feeding. Linoleic acid and unsaturated fatty acids in milk in organic feeding was higher than those in conventional feeding and properties of milk products were improved in organic milk compared to conventional milk.

Feeding habits of Acentrogobius pellidebilis collected from in an eelgrass(Zostera marina) bed from January to December 2006 were studied. A. pellidebilis ranged form 1.6 to 6.9 cm in standard length were determined. A. pellidebilis was a carnivore which consumed mainly copepods, polychaetes, and amphipods. Its diets included small quantities of isopods, seaweeds, nematods, and gastropods. It showed ontogenetic changes in feeding habits. Smaller individuals (<2.0cm SL) fed mainly on copepods. While the consumption of copepods decreased with increasing fish size, the consumption of polychaetes and amphipods were increased. Dietary breadth index was varied with fish size.

본 연구는 왕피천의 섭식기능군을 이용해 군집분석을 실시하기 위한 것으로 2007년 10월부터 2008년 5월까지 총 4회에 걸쳐 조사를 수행하고 그 결과를 분석하였다. 조사시기동안 출현한 저서성 대형무척추동물은 총 4문 6강 16목 58과 138종이었으며, EPT index 값은 61.59%로 매우 높게 나타나 왕피천의 하천생태계는 매우 청정하고 건강한 것으로 분석되었다. 왕피천의 하천지점별 섭식기능군을 살펴본 결과 긁어먹는 무리와 주워먹는 무리가 지류에 비해 우점하고 있는 일반적인 하천 중류의 특징을 보이는 그룹과 썰어먹는 무리가 본류에 비해 높은 비율을 차지하고 있는 상류 특성을 보이는 지류의 그룹인 두 개의 그룹으로 나뉘어졌다. 유사도 지수를 근거로 각 지점별 클러스터 분석을 실시해 본 결과, 자연 상태의 비교란 지역(A그룹)과 제방 및 보 등에 의한 인위적 교란지역(B그룹; 지점 8, 11)으로 그룹화 되었다. 또한 비교란 지역은 본류(지점 1, 2, 3, 4, 7) 및 지류(지점 5, 6, 9, 10)의 특성을 갖는 두개의 그룹으로 분류됨을 확인 할 수 있었다.

evolution of multicellular organisms. Especially in insects, a large amount of bacterial symbionts are illustrated. Among the insect taxonomic group, sap feeding insects, mostly Heteroptera, that feeds on poor nutrient sources from plants, have developed special organs where symbiotic bacteria can reside. The bacteria mainly provide the host insects with some essential amino acids. This has built evolutionarily unbreakable tie between the symbionts and the their hosts. Here we present an obligate symbiont from Lycorma delicatula (Heteroptera: Fulgoridae) being thought to invade the Korean penninsula recently. Based on the partial 16s rRNA gene sequence, the symbiotic bacterium is identified as Cadidatus Sulcia mullleri. The Genbank data indicates that the bacterium is found in various heteropteran families. This may imply the acquisition of the bacterium precedes the hosts' divergence, though there is an alternative postulation. We discuss its distribution in the sap-feeding insects and potential role on survival of L. delicatula and paratransgenetic application of the bacterium in controlling L. delicatula.

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

We present the first comprehensive cladistic analysis of Miridae, the plant bugs, based on analysis of 3935 base pairs of mitochondrial (16S, COI) and nuclear (18S, 28SD3) DNA for 91 taxa in seven subfamilies. We analyzed the data using maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BI) phylogenetic frameworks. A Bayesian relaxed molecular clock was used to examine divergence times, and ancestral feeding habits were reconstructed using parsimony and a Bayesian approach. Clades recovered in all analyses are as follows: Cimicomorpha, Miroidea, and Miridae; Bryocorinae: Bryocorini; Stenodemini; Mirinae; Deraeocorinae (Clevinemini + Deraeocorini); Cylapinae; Isometopinae; Bryocorinae: Dicyphini; Orthotylini; Phylinae (Phylini + Pilophorini), and Phylinae is sister-group to all the remaining mirid taxa. These results are largely congruent with former hypotheses based on morphological data with respect to the monophyly of various subfamilies and tribes however, our results indicate that the subfamily Bryocorinae is not a monophyletic group as the two tribes, Dicypini and Bryocorini, were separated in our phylogeny. Ancestral feeding state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed phytophagy at the root of the Miridae.

본 연구는 답리작에서 보리를 재배하여 사일리지를 조제하고 거세 한우에게 청보리 사일리지를 급여하여 성장, 사료이용성, 도체특성 및 육질개선 효과를 구명하고자 전북 정읍 농가에서 4개월령 거세 한우 육성우 21두를 공시하여 27개령까지 사육하였다. 대조구(일반볏짚구), 청보리 사일리지구, 생볏짚 사일리지+청보리 사일리지구 등 3처리를 두었으며, 배합사료는 육성기는 체중의 1.5%, 비육전기는 1.8%, 비육후기는 자유 채식하도록 하였고, 조사료는 사육기간

This study was carried out between 2008 and 2009 in four dairy farms to investigate the effect of feeding of whole crop barley silage on the reproductive performance of Holstein heifers. Two diets, mixed hay or whole crop barley silage separately from concentrate were fed 6-month old Holstein heifers (=37). In control group (=CON), heifers (n=16) were fed 6 kg (/head) mixed hay and 4 kg (/head) commercial diet. In whole crop barley silage group (=WBS), heifers (n=21) were fed 10 kg (/head) whole crop barley silage, 4 kg (/head) mixed hay and 2 kg (/head) commercial diet. To manage body weight gain, the body condition score of heifers were measured every month. The results obtained were as follows: 1. Body weight in CON and WBS heifers at 13-, 14-, 15- and 17-month old were 340±17.9 and 342±13.6 kg, 356±15.7 and 366±14.7 kg, 382±13.1 and 387±14.4 kg, and 429±15.0 and 417±10.3 kg, respectively. 2. Body condition score in CON and WBS heifers at 9-, 12-, 15- and 17-month old were 2.88±0.04 and 2.80±0.04, 2.88±0.04 and 2.80±0.04, 2.89±0.08 and 3.00±0.07, and 2.89±0.08 and 3.00±0.07, respectively. 3. Average age of sexual maturity in CON and WBS heifers were 437.3±9.9days and 939.6±12.5days, WBS group heifers were significantly shorter (p<0.05) than CON group heifers. 4. First-service conception rates in CON or WBS group were 81.3% (13/16) and 66.7% (14/21), respectively, and cumulative conception rate to 2nd artificial insemination were 87.5% for CON and 85.7% for WBS group. Conception rate was not different between treatments

s study was carried out between November 2008 and October 2009 in the experimental farm of Chunnam National University to investigate the effect of feeding of Sudangrass silage on the reproductive performance of Hanwoo cows. Two diets, rice straw or Sudangrass silage separately from concentrate were fed 19 Hanwoo cows. In control group (=CON), cows (n=9) were fed 5 kg (/head) rice straw and 3 kg (/head) commercial diet. In Sudangrass silage group (=SGS), cows (n=10) were fed 12.5 kg (/head) Sudangrass silage and 1 kg (/head) commercial diet. 1. Days to post-partum insemination were 78.8±25.3 days for CON and 84.7±24.6 days for SGS group and days to post-partum conception in CON or SGS were 90.1±24.1 and 87.7±26.8 days, respectively. 2. Post-partum conception rates for first service in CON or SGS was 66.7% and 90.0%, respectively, and caving interval was 375.1±46.7 days for CON and 370.2±34.4 days for SGS group. 3. Body Condition Scores of Hanwoo cows at artificial insemination was 5.36 for CON group and 4.93 for SGS group.

본 연구는 부산물 혼합 silage 사료 급여가 한우 비육전기 거세우의 채식 및 반추행동에 미치는 영향을 규명하기 위하여 실시하였다. 시험에 사용한 공시한우는 21개월령 총 12두를 3처리로 배치하였으며, 이 때 처리구는 관행구 (C구: 농후사료+ 볏짚 자유채식)와 부산물 혼합 silage 구를 2처리 (T1: 농후사료+부산물 혼합 silage 제한급여 (1 kg) + 볏짚 자유채식, T2: 농후사료+부산물 혼합 silage 자유채식)로 하여 48시간

본 연구는 조사료원으로 청보리 사일리지 급여시 농후사료 급여수준이 육성기 흑염소의 가소화 영양소 섭취량, 영양소 이용율 및 질소축적율에 미치는 영향을 조사하였다. 시험구는 4처리구 (볏짚+농후사료 체중의 2.0%, 청보리사일리지 + 농후사료 체중의 2.0%, 체중의 1.5%, 체중의 1.0%)로 나누어 처리구당 3두씩 라틴방각법으로 시험을 실시하였다. 시험기간 동안의 1일 두당 가소화 조단백질 섭취량은 대조구, 청보리 사일리지에 농후사료를 각각 체중의

본 연구는 동계사료 작물과 혼파한 총체보리사일리지를 흑염소에 급여하였을 때, 사료섭취량, 일당증체량, 영양소 소화율 및 질소 축적율에 미치는 영향을 조사하여 흑염소에 대한 혼파 총체보리의 사료가치 평가 자료로 활용하는 데 목적을 두고 실시하였다. 공시가축은 흑염소 12두 (male)를 4처리구 {총체보리 단파 사일리지 첨가구 (T1), 헤어리베치 혼파 사일리지 첨가구 (T2), 사료용 완두 (forage pea) 혼파 사일리지 첨가구 (T3) 및 이탈리

The present study was carried out to investigate the effects of feeding dietary legislation sulfur on the lipid levels of chicken meat and growth performance in broilers. A total of 180 male broilers (Ross strain 308) were randomly assigned into three treated groups with triplicate of 20 birds each, T1 (control group), T2 (antibiotics 8 ppm), and T3 (0.3% sulfur premix containing 30% of sulfur) groups. The body weight gains over a 3-wk period were significantly higher in T2 and T3 groups than the T1 group, but not different between T2 and T3. The dressing percentage, weight of thigh meat, breast muscle and bursa of F were significantly high in T2 and T3 groups compared to the T1 group, but not different between T2 and T3. The weight of abdominal fat was significantly higher in T3 group than in T1 and T2 groups. The cholesterol content of the whole chicken meat was significantly lower in the T3 group as compared to the T1 and T2 groups. The oleic acid (18:1n-9) content of whole chicken meat from the T3 group was higher than that of the other groups. The results of this study suggests that feeding dietary sulfur as an antibiotic replacement may increase the oleic acid and reduce the cholesterol in chicken meat while improving the carcass characteristics and growth performance in broiler chickens