This research was conducted to compare the dry matter production and the yield productivity among nine soybean cultivars by measuring the photosynthetic ability of the middle and lower leaves at the flowering and the seed development stages. The leaf greenness(SPAD value) were ranged as 32-42 at the flowering stage. Also, They were ranged as 25-40 and 38-51 at the fifth leaf and the seventh leaf, respectively. The photosynthetic ability at the flowering and the seed development stage showed significant differences among soybean cultivars, and the photosynthetic ability at the seed development stage showed higher difference among cultivars than the flowering stage. The variation of the photosynthetic ability at the flowering and the seed development stage also was significant among cultivars. The light saturation point at the flowering stage was about 1500 ~mu mol m-2 s-1 PAR, and the seed development stage was about 1000 ~mu mol m-2 s-1 PAR. The photosynthesis showed the high negative correlation with the leaf area and the positive correlation with the leaf area ratio. Also, photosynthesis at seed development stage showed positive correlation with grain yields but there was not significant between photosynthesis and yields at flowering stage..

산초나무는 잎에서 총 52개, 열매에서 48개 향기 성분이 동정되었으며, 4개 지역에서 잎의 주요 공통 성분은 (E)-2-hexenal, α-pinene, (Z)-ocimene+limonene, estragole, germacrene-d 이였으며, 열매에서는 estragole이였다. Hexanal, (Z)-3-hexenol, (E)-2-hexenal, n-hexanol 성분은 잎에서만 검출되었고, undecanone 성분은 열매에서만 나타났다. 지역간 큰 차이를 보인 잎의 정유성분은 hexanal, azulene이었고, 열매에서는 (Z)-ocimene+limonene이였다. 초피나무 잎에서 총 30개, 열매에서 27개 향기 성분이 동정되었으며, 잎의 주요 공통 성분은 백양사와 내장사지역의 성분이 α-pinene, β-phellandrene, 1,8-cineole, citronellal이였고, 통도사 지역은 (Z)-3-hexenol, (E)-2-hexenal, α-pinene, myrcene이 주요성분 이였다. 백양사와 내장사에서 채취한 열매의 공통 성분은 myrcene, (Z)-ocimene+limonene, β-phellandrene이였고, 통도사에서는 β-phellandrene, citronellal, geranyl acetate이 주성분이였다.

This study was conducted to determine the contents of heavy metals in leaves of roadside trees according to different growth stages in Daegu city. The orders of heavy metal contents in leaves of roadside tree and soil were Fe＞Mn＞Zn＞Pb＞Cu＞Cr＞Cd and Fe＞Mn＞Zn＞Cu＞Pb＞Cr＞Cd, respectively. The contents of heavy metals in leaves of roadside trees and soil showed an increasing tendency as the levels of traffic volume increased. The contents of heavy metals in leaves on October were higher than those on May. Zelkova serrata and Ginkgo biloba showed high contents of Cr, Cd and Pb.

The effects on photosynthesis of NaCl(0, 0.2, 0.4, 0.6, 0.8 or 1.0 M) were examined in etiolated barley seedlings. Chlorophyll(Chl) a, Chl b and carotenoid contents, Chl a fluorescence and quenching coefficients of Chl fluorescence have been determined in the primary leaves of etiolated barley seedlings cultivated under low light(60 μ㏖ m-2s-1). Chl a, b, and carotenoid contents were decreased remarkably in comparison with the control at 0.4 M NaCl. However, the value of Fo and Fv were decreased at 0.6 M NaCl and the ratio of Fv/Fm were deceased at 1.0 M NaCl. Chlorophyll synthesis was seriously inhibited from 0.4 M NaCl, and the photosynthetic electron transport system was inhibited from 0.6 M NaCl. Quantum of photosystem II reaction center was inhibited at 1.0 M NaCl. The effects of NaCl on the Chl content were raised in a 6 hrs, but the effects of NaCl on the value of Fo, Fv and Fv/Fm were raised in 30 hrs. The value of qP was decreased in comparison with the control at all concentrations, but there was a small change in the value qE. These results provide evidence that NaCl inhibited effects of various concentration of NaCl were inhibited quinone redox, however, proton gradient between thylakoid membranes was little damaged.

The variation of alternative and cytochrome respiration during ripening in rice leaves (Oryza sativa L. cv. Takanari) was examined. The capacity of both respiration pathway was measured by inhibitor titration method using gas-phase oxygen electrodes. The alternative respiration rate decreased from 31.3% of the total respiration rate at around heading to 11.7% at 34 days after heading in the first fully expanded leaf from the top, and further to 6.4 % at 34 days after heading in the fourth leaf from the top. In contrast, the proportion of cytochrome respiration to total respiration increased with leaf senescence. The possible cause of alternative respiration as either an increase in inefficient respiration or a decrease in growth efficiency during ripening was discussed.

밤잎의 기능성 식품 및 의약품으로서의 활용도를 높이기 위한 일환으로 밤잎의 화학성분, 항산화 및 항균활성을 측정한 결과는 다음과 같다. 일반성분은 총당 11.95%, 조지방 11.50%, 조섬유 10.11%, 조단백질 7.50% 및 회분이 1.79%순으로 나타났으며, 주요 무기성분으로는 Ca과 K으로 그 함량은 각각 215.7 mg%와 196.6 mg%로 나타났다. 비타민 C의 함량은 12.5 mg%였으며, 유리당은 glucose 3.33%, f

황칠나무 잎의 추출물의 면역활성증진 실험에서 인간정상 간세포의 경우 모든 추출물이 1.0 Mg/ml의 농도에서 최고 26%이하의 세포독성을 나타내었다. 서로 다른 4가지의 암세포주(MCF7, A549, Hep3B, AGS)에서 50%이상의 저해율을 나타냈고, 정상 세포의 결과와 암세포의 저해율을 비로 나타낸 selectivity의 측정에서 모든 암세포주가 1.5이상의 사멸도를 나타내었고 전체적으로 에탄올 추출물의 효과가 가장 좋았다. 에탄올 추출물의 경우에서 인감 유방암 세포주(MCF7)와 인간 간암세포주(Hep3B)의 경우에서 1.0 mg/ml농도에서 각각 65%와 67%의 저해율을 기록했다. 면역세포 실험에서 에탄올 추출물이 1.0 mg/ml의 농도에서 B세포는 1.22배, T세포는 1.27배의 촉진 활성을 보였고, 6일 동안측정한 cytokines(IL-6, TNF-α)의 양도 에탄올 추출물의 경우 T cell의 경우 IL-6은 94pg/m1, TNF-α은 75pg/ml로 증가하는 것을 알 수 있었다. 이상의 결과로 볼 때 추출 열과 추출용매 등에 의해 황칠나무 잎에 존재하는 여러 가지 유용성분들이 풍부하게 존재하며, crude추출물 중에 에탄올 추출물이 면역활성에서 좋은 효과를 보였다는 것을 알 수가 있었고, 이를 통해서 황칠나무 잎을 이용한 기능성 식품으로의 연구 개발이 통해서 충분히 그 가치가 있다는 것을 알 수 있었다.

야콘(Polynmia sonchilolia)의 식품가공에의 적용 확대를 위해 그것들의 신선한 잎, 줄기, 괴근과 건조 잎과 줄기 및 후숙 후의 괴근의 화학적 성분들을 조사하였다. 수분함량은 신선한 잎의 경우 83.38%에서 건조 후 18.08%이었고, 줄기의 경우 92.30%에서 건조 후 27.97%로 크게 감소하였으며, 괴근의 경우 신선한 것은 89.52%이었으나 후숙 후 86.99%로 수분감소가 적게 나타났다. 잎의 지방, 단백질, 가용분,

품질관련 성분 중 총질소, 총아미노산, 엽록소 및 비타민 C는 CO2 gas 3시간 혐기처리가 타처리에 비해 함량이 약간 많았으며, 탄닌은 공기 처리가 카페인은 진공처리에서 함량이 약간 많은 경향이었다. 유리당은 CO2 gas 처리가 2,050mg/100g으로 타처리에 비해 31~449mg/100g정도 많았다. 혐기처리시 GABA와 alanine이 축적되는데, GABA함량은 N2 CO2 gas 처리가 무처리 (350mg/100g)에 비해 215mg/100g과 264mg/100g으로 6~8배 많았다. 제다품질은 무처리 80.4점에 비해 N2, CO2 gas 처리 각각 80.0, 80.3점으로 차이가 거의 없었으나, 공기 및 진공처리는 각각 78.3, 78.9점으로 약간 품질이 저하되는 경향이었다. N2나 CO2 gas처리 후 제다된 녹차는 기능성분인 GABA함량이 무처리에 비해 6~8배 많고, 제다 품질도 양호하였다.

질소비료를 3~7회 분시하여 옥로차의 수량 및 품질을 분석한 결과는 다음과 같다. 5회 분시가 타처리에 비해 생육과 수량이 많고 타처리에 비해 총질소, 총아미노산, 비타민C 함량이 약간 많았으며 탄닌, 카페인, 엽록소, 총유리당 함량은 3회분시를 제외하고 4~7회 분시에서 거의 차이가 없었다. 지방산 함량은 2,888~2,933mg/100g이었고, 특히 5회 분시에서는 oleic acid, linoleic acid, linolenic acild 함량이 가장 많았다. 옥로차 감칠맛 성분인 theanine을 포함해 16종이 아미노산을 분리 정량한 결과 5회 분시가 타처리에 비해 theanine 함량이 72~513mg/100g더 많았다. 관능평가에서 3회 분시가 80.2점으로서 다른 처리보다 낮았고, 5회 처리가 84.7점으로 가장 높았다. 결론적으로 5회 분시처리가 다른처리에 비해 수량도 양호하고 차 품질도 우수하여 옥로차의 적정 분시첫수라고 생각된다.

The induction of enzymes and the accumulation of their end products associated with self-defense mechanism in rice were investigated. When rice leaves were irradiated with UV light, activities of diterpene cyclase, phenylalanine ammonia-lyase (PAL), and cinnamic acid 4-hydroxylase (CA4H) were induced and rice phytoalexin, momilactone A was accumulated. The content of p-coumaric acid in rice leaves was closely correlated with self-defense or allelopathic potential against barnyardgrass. UV-challenged rice leaves gave rise to the inhibition of barnyardgrass growth

밤나무(Castanea crenat)의 지상부를 대상으로 DPPH free radical 소거법을 이용하여 2종의 항산화 활성물질을 분리하였다. 분리된 활성물질은 NMR과 mass 분석에 의하여 quercitrin, isoquercitrin으로 밝혀졌다. 두 화합물의 DPPH free radical 소거법에 의한 항산화 활성 (RC50 : 12μg)은 BHA (14 μg)와 α-tocopherol (12μg)과 비슷하였다.

We compared microstructural features of the ordered cell and disordered leaves in Citrus junos Sieb. by electron microscopy. In the cell of the ordered leaves, many chloroplasts and large vacuoles were particularly observed. Also a lot of vessel, companion cell and big nucleus were presented in vascular bundle regions. The mitochondria and the other organelles were interspersed among the chloroplasts in a thin, peripheral layer of cytoplasm. The chloroplast possessed typical grana and intergranal lamellae, numerous starch grains and a few small osmophilic globules. Besides, microbodies were closely associated with the mitochondria and the chloroplast. The process of the formation of the secondary cell wall from primary cell wall was observed the vessel elements, the tonoplast wall and the secondary cell wall. It was observed that the oil sac with the unique perfume distributed the adjacent cell wall. In the cell of disordered leaves, the all of the organelles were thrust toward the cell wall due to the fusion of vacuoles in the cells. It was observed that a lot of the very small particles spreaded in the cytoplasm. The loss of unique perfume of the leaves was resulted in the destruction of the oil sac. Also, there was not observed grana, lamellae, starch and osmophillic globules in the chloroplast. The small distributed organelles was not observed but the elongation of the cell wall was proceed no longer. Therefore, the plasma membrane diverged from the cell wall. All of organelles in the cell had poor function and deformation. A massive vacuole was fulfilled in single cell and the vacuole contains a lot of large and small particles. The organelles were presented on the side of the cell wall according to the enlargement of vacuole and they were observed to be breakdown.

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

To study the potential of the Zanthoxylum schinifolium and Z. piperitum leaves, as raw materials for functional food and medicine, apart from male and female, chemical components were carried out. Among general components of sancho and chopi leaves, moisture and crude protein were higher sancho leaf than chopi leaf, but total sugar and crude fat were higher chopi leaf than sancho leaf and the components of major minerals were K, Ca, Mg and Na. Among free sugars, glucose(0.24% and 0.21%) and sucrose(0.19% and 0.27%) were the highest contents in sancho and chopi leaves(male and female), respectively. The organic acid were isolation and identification as malic acid and citric acid, citric acid is higher than malic acid. The total amino acid of sancho and chopi leaves contained proline and glutamic acid in male and female sancho and female chopi leaves, glutamic acid and aspartic acid in male chopi leaf highly in order. The fatty acid contents of four samples were high 15.16%, 9.76%, 8.78% and 9.29% of linolenic acid, respectively. Among many volatile compounds, limonene(13.25% and 19.16%) and citronellal(34.37% and 29.66%) were predominant flavor compounds in sancho and chopi leaves(male and female), respectively.

Comparing and analysing the productivity and the main ingredients of persimmon leaves by cultivars were conducted to improve the quality and productivity of the per-simmon leaf tea. As a result of the survey, Dungsi was decidedly good in growth co-ndition of survival rate, stem diameter and the number of shoots etc. Also , DUngsi had the highest leaf productivity of 567 leaves and the leaf weight of 1.98 kg per one plant after three years of planting . On accounts of productivity, ingredient analysis and panel test etc. we collectively through that Dungsi was the best cutltivar for the persimmon leaf tea.

1. 콩잎의 세균밀도는 4.60×102~ 9.10×105CFU/ cm2으로, 생육단계가 진전됨에 따라 세균밀도가 증가하는 경향이었다. 2. 콩나물 부패 세균의 밀도는 콩잎에서 0~5.00×103CFU/ cm2으로, 부패세균의 밀도는 생육단계에 관련이 없었으나 재배지역과는 관련이 있었다. 3. 나물 콩 품종과 콩나물 부패세균의 밀도는 품종과 관련이 적었으며 생육단계와 작물의 부위에 따라 변이가 심하였다. 4. 콩잎에서 분리된 콩나물 부패세균은 Erwinia cypripedii, E. carotovora subsp. carotovora, Xanthomonas campestris pv. glycines, Staphylococcus sp., Micrococcus sp. 이며, E. carotovora subsp. carotovora, X. campestris pv. glycines가 밀도가 높았다.다.

Volatile components were extracted from leaves of two Boxthorn (Lycium chinense M.) cultivars by using simultaneous distillation and extraction, analyzed by gas chromatography-mass spectrometry. Seventy components were identified : 13 acids, 15 alcohols, 18 hydrocarbons, 13 carbonyls, three esters, three ionones, and five others. The principal volatile components (and their peak area percentage) were n-pentanol (11.2～30.2%), phytol (14.5～28.3%), hexadecanoic acid (13.5～17.1%) 2,3-dihydrobenzofuran (1.5～4.2%), benzyl alcohol (1.9-4.8%), phenylacetaldehyde (1.8～3.2%), and octadecadienoic acid (1.7～10.7%). Fresh leaves showed much higher peak area than that of dried leaf in n-pentanol, n-hexanol, cis-2-penten-l-ol, cis-3-hexen-l-ol, benzyl alcohol, and β -phenylethyl alcohol, while dried leaves showed much higher content than that of fresh leaves in 9-hydroxytheaspran A, octadecanoic acid and octadecadienic acid.